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The map label for this gene is rutR [H]

Identifier: 77457827

GI number: 77457827

Start: 1787518

End: 1788123

Strand: Reverse

Name: rutR [H]

Synonym: Pfl01_1600

Alternate gene names: 77457827

Gene position: 1788123-1787518 (Counterclockwise)

Preceding gene: 77457829

Following gene: 77457825

Centisome position: 27.77

GC content: 60.89

Gene sequence:

>606_bases
ATGAGCACAATCCGCGAGCGCAACAAAGAACTGATCCTGCGTGCAGCCAGTGAAGAGTTTGCCGACAAGGGCTTCGCTGC
GACCAAAACCAGCGACATCGCCGCCAAGGCAGGACTGCCCAAGCCCAACGTCTATTACTACTTCAAGTCCAAGGAAAACC
TCTACCGCGAGGTGCTGGAAAGTATCATCGTGCCGATCCTGCAGGCCTCGACCCCGTTCAACCCGGACGGCGTACCGAGC
GAAGTGCTGAGCGGTTACATCCGCTCGAAGATCCGCATCTCTCGCGACCTGCCCTTCGCCTCCAAGGTGTTCGCCAGCGA
AATCATGCACGGCGCCCCGCACCTGAGCGCCGACCTGGTCGAGCAGCTCAACGGCCAGGCCAAGCACAACATCGACTGCA
TCCAGACCTGGATCGACCGCGGCCAGATCGCCCCGATCGACCCCAACCACCTGATGTTCAGCATCTGGGCCGCAACCCAG
ACCTACGCCGACTTCGACTGGCAGATCTCCGCCGTCACCGGCAAGGACAAGCTCGACGAAGCCGACTATGAAGCGGCAGC
ACAAACCATCATCCGGTTGGTGCTCAAGGGCTGCGAGCCGGACTGA

Upstream 100 bases:

>100_bases
GCATACCGCGACTGCAGGCCACACGGGGCATTGTTGAGCGCCGCGCGAGGCCGGTGCTACCATGCGCAACCGCCAGAACA
GGCAAGGAACCACCGGGTTT

Downstream 100 bases:

>100_bases
AGACCGCCCTCCAGACACAAACAAGCCCGCCCTCACATCATCCTGTGAGGGCGGGCTTGTGCGTTTCAGCGACTATCCGG
TTTTAGCCACTCAGGCCGCC

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MSTIRERNKELILRAASEEFADKGFAATKTSDIAAKAGLPKPNVYYYFKSKENLYREVLESIIVPILQASTPFNPDGVPS
EVLSGYIRSKIRISRDLPFASKVFASEIMHGAPHLSADLVEQLNGQAKHNIDCIQTWIDRGQIAPIDPNHLMFSIWAATQ
TYADFDWQISAVTGKDKLDEADYEAAAQTIIRLVLKGCEPD

Sequences:

>Translated_201_residues
MSTIRERNKELILRAASEEFADKGFAATKTSDIAAKAGLPKPNVYYYFKSKENLYREVLESIIVPILQASTPFNPDGVPS
EVLSGYIRSKIRISRDLPFASKVFASEIMHGAPHLSADLVEQLNGQAKHNIDCIQTWIDRGQIAPIDPNHLMFSIWAATQ
TYADFDWQISAVTGKDKLDEADYEAAAQTIIRLVLKGCEPD
>Mature_200_residues
STIRERNKELILRAASEEFADKGFAATKTSDIAAKAGLPKPNVYYYFKSKENLYREVLESIIVPILQASTPFNPDGVPSE
VLSGYIRSKIRISRDLPFASKVFASEIMHGAPHLSADLVEQLNGQAKHNIDCIQTWIDRGQIAPIDPNHLMFSIWAATQT
YADFDWQISAVTGKDKLDEADYEAAAQTIIRLVLKGCEPD

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=193, Percent_Identity=40.4145077720207, Blast_Score=147, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 22403; Mature: 22271

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIRERNKELILRAASEEFADKGFAATKTSDIAAKAGLPKPNVYYYFKSKENLYREVLE
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEECCHHHHHHHHHH
SIIVPILQASTPFNPDGVPSEVLSGYIRSKIRISRDLPFASKVFASEIMHGAPHLSADLV
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHH
EQLNGQAKHNIDCIQTWIDRGQIAPIDPNHLMFSIWAATQTYADFDWQISAVTGKDKLDE
HHHCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHCCCCCEEEEEECCCCCCCH
ADYEAAAQTIIRLVLKGCEPD
HHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STIRERNKELILRAASEEFADKGFAATKTSDIAAKAGLPKPNVYYYFKSKENLYREVLE
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEECCHHHHHHHHHH
SIIVPILQASTPFNPDGVPSEVLSGYIRSKIRISRDLPFASKVFASEIMHGAPHLSADLV
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHH
EQLNGQAKHNIDCIQTWIDRGQIAPIDPNHLMFSIWAATQTYADFDWQISAVTGKDKLDE
HHHCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHCCCCCEEEEEECCCCCCCH
ADYEAAAQTIIRLVLKGCEPD
HHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]