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The map label for this gene is gcl [H]

Identifier: 77457825

GI number: 77457825

Start: 1784943

End: 1786718

Strand: Reverse

Name: gcl [H]

Synonym: Pfl01_1598

Alternate gene names: 77457825

Gene position: 1786718-1784943 (Counterclockwise)

Preceding gene: 77457827

Following gene: 77457824

Centisome position: 27.75

GC content: 62.27

Gene sequence:

>1776_bases
ATGAGCAAAATGAGAGCAATCGAAGCCGCCGTTCTGGTGATGCGCCGTGAAGGGGTTGATACCGCTTTTGGCATTCCAGG
CGCTGCAATCAACCCGCTGTACTCCGCCCTGCAGAAAGTCGGTGGCATCGATCACGTCCTCGCTCGCCACGTTGAAGGCG
CCTCGCACATGGCCGAGGGCTACACCCGCACCAAGGCCGGCAACATCGGCGTGTGCATCGGTACTTCCGGCCCGGCCGGT
ACCGACATGGTCACCGGTCTGTACAGCGCCTCCGCGGACTCGATCCCGATCCTCTGCATCACCGGGCAAGCCCCCCGCGC
CCGTATGCACAAGGAAGACTTCCAGGCCGTCGACATCACCAGCATCGTCAAGCCGGTGACCAAGTGGGCGACCACCGTTC
TGGAGCCGGGCCAGGTGCCTTACGCTTTCCAGAAAGCCTTCTACGAAATGCGCTCCGGCCGTCCGGGCCCGGTGCTGATC
GACCTGCCGTTCGACGTGCAGATGGCTGAAATCGAATTCGACATCGACGCCTACCAGCCACTGCCGCTGGCCAAGCCGAG
CGCCACCCGCGTGCAAGTCGAAAAAGCCCTGGCGATGCTGGATCAGGCCGAGCGTCCATTGCTGGTGGCCGGCGGCGGCA
TCATCAACGCCGACGCCAGCGAGCTGCTGGTCGAGTTCGCCGAGCTGACCGGCATTCCAGTGATCCCGACCCTGATGGGC
TGGGGCACCATTCCGGACGATCACCCGCTGATGGTCGGCATGGTCGGTCTGCAGACTTCGCACCGCTATGGCAACGCCAC
CCTGCTCAAGTCCGACGTGGTACTGGGTGTCGGCAACCGCTGGGCCAACCGTCACACCGGTTCGGTCGACGTTTACACCG
AAGGCCGCAAGTTCATTCACGTCGACATCGAACCGACCCAGATCGGCCGCGTGTTCACCCCGGACCTGGGCATCGTTTCC
GACGCCGCTGCCGCGCTGACCGTGTTCATCGAAGTCGCCCGTGAATGGCAAGCCGCCGGCAAACTGAAAAACCGCAGTGC
CTGGCTGCAGGATTGCCAGCAGCGCAAAGCCAGCCTGCAACGCAAGACCCACTTCGACAATGTGCCGGTCAAGCCGCAGC
GCGTTTACGAAGAAATGAACCAGGTGTTCGGCAAGGACACCTGCTACGTCAGCACGATTGGTCTGTCGCAGATTGCCGGC
GCGCAGTTCCTGCACGTCTACAAGCCGCGTCACTGGATCAACTGCGGCCAGGCCGGCCCGTTGGGCTGGACCATTCCGGC
AGCGCTGGGCGTGGTGAAGGCCGATCCGAACCGTAAAGTCGTGGCCCTGTCGGGGGACTATGACTTCCAGTTCATGATCG
AAGAGCTGGCGGTCGGCGCTCAGTTCAAACTGCCGTACATCCACGTCGTGGTGAACAACTCGTACCTGGGGCTGATCCGT
CAGGCCCAGCGCGGGTTCGATATGGACTACTGCGTGCAGCTGTCCTTCGATAACCTGAACGCGCCGGAACTCAACGGTTA
TGGTGTCGACCACGTCGCAGTCGCCGAAGGCCTGGGCTGTAAAGCGCTGCGTGTGTTCGAACCGGCTGACATCGCCCCTG
CCCTGCGCAAGGCCGAAGAGCTGATCGAAGAGTTCAAGGTGCCGGTGATCGTCGAGATCATTCTGGAGCGTGTGACCAAC
ATCTCCATGGGGACCGAGATCAACGCCGTCAACGAATTCGAAGACCTGGCGCTGGTCGGCAACGATGCGCCTACCGCGAT
TTCGTTGCTCGACTGA

Upstream 100 bases:

>100_bases
AGGCGCTTGAGAAAATGGATTGACCTGCGCCGTCCGCCGTGAATACACTCTGCGCAAAGCCACTTGTATACAATTACAAA
ACGTAAAGAGGCACAAAACC

Downstream 100 bases:

>100_bases
ACGTAACCGGCTCCCTCTCCCTCCGGGAGAGGGGACTTGATCGCGTTCAGTCTGACCAACCGTGTCAAAACTGTTTTAGG
AGACCACCATGCCGCGTTTC

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MSKMRAIEAAVLVMRREGVDTAFGIPGAAINPLYSALQKVGGIDHVLARHVEGASHMAEGYTRTKAGNIGVCIGTSGPAG
TDMVTGLYSASADSIPILCITGQAPRARMHKEDFQAVDITSIVKPVTKWATTVLEPGQVPYAFQKAFYEMRSGRPGPVLI
DLPFDVQMAEIEFDIDAYQPLPLAKPSATRVQVEKALAMLDQAERPLLVAGGGIINADASELLVEFAELTGIPVIPTLMG
WGTIPDDHPLMVGMVGLQTSHRYGNATLLKSDVVLGVGNRWANRHTGSVDVYTEGRKFIHVDIEPTQIGRVFTPDLGIVS
DAAAALTVFIEVAREWQAAGKLKNRSAWLQDCQQRKASLQRKTHFDNVPVKPQRVYEEMNQVFGKDTCYVSTIGLSQIAG
AQFLHVYKPRHWINCGQAGPLGWTIPAALGVVKADPNRKVVALSGDYDFQFMIEELAVGAQFKLPYIHVVVNNSYLGLIR
QAQRGFDMDYCVQLSFDNLNAPELNGYGVDHVAVAEGLGCKALRVFEPADIAPALRKAEELIEEFKVPVIVEIILERVTN
ISMGTEINAVNEFEDLALVGNDAPTAISLLD

Sequences:

>Translated_591_residues
MSKMRAIEAAVLVMRREGVDTAFGIPGAAINPLYSALQKVGGIDHVLARHVEGASHMAEGYTRTKAGNIGVCIGTSGPAG
TDMVTGLYSASADSIPILCITGQAPRARMHKEDFQAVDITSIVKPVTKWATTVLEPGQVPYAFQKAFYEMRSGRPGPVLI
DLPFDVQMAEIEFDIDAYQPLPLAKPSATRVQVEKALAMLDQAERPLLVAGGGIINADASELLVEFAELTGIPVIPTLMG
WGTIPDDHPLMVGMVGLQTSHRYGNATLLKSDVVLGVGNRWANRHTGSVDVYTEGRKFIHVDIEPTQIGRVFTPDLGIVS
DAAAALTVFIEVAREWQAAGKLKNRSAWLQDCQQRKASLQRKTHFDNVPVKPQRVYEEMNQVFGKDTCYVSTIGLSQIAG
AQFLHVYKPRHWINCGQAGPLGWTIPAALGVVKADPNRKVVALSGDYDFQFMIEELAVGAQFKLPYIHVVVNNSYLGLIR
QAQRGFDMDYCVQLSFDNLNAPELNGYGVDHVAVAEGLGCKALRVFEPADIAPALRKAEELIEEFKVPVIVEIILERVTN
ISMGTEINAVNEFEDLALVGNDAPTAISLLD
>Mature_590_residues
SKMRAIEAAVLVMRREGVDTAFGIPGAAINPLYSALQKVGGIDHVLARHVEGASHMAEGYTRTKAGNIGVCIGTSGPAGT
DMVTGLYSASADSIPILCITGQAPRARMHKEDFQAVDITSIVKPVTKWATTVLEPGQVPYAFQKAFYEMRSGRPGPVLID
LPFDVQMAEIEFDIDAYQPLPLAKPSATRVQVEKALAMLDQAERPLLVAGGGIINADASELLVEFAELTGIPVIPTLMGW
GTIPDDHPLMVGMVGLQTSHRYGNATLLKSDVVLGVGNRWANRHTGSVDVYTEGRKFIHVDIEPTQIGRVFTPDLGIVSD
AAAALTVFIEVAREWQAAGKLKNRSAWLQDCQQRKASLQRKTHFDNVPVKPQRVYEEMNQVFGKDTCYVSTIGLSQIAGA
QFLHVYKPRHWINCGQAGPLGWTIPAALGVVKADPNRKVVALSGDYDFQFMIEELAVGAQFKLPYIHVVVNNSYLGLIRQ
AQRGFDMDYCVQLSFDNLNAPELNGYGVDHVAVAEGLGCKALRVFEPADIAPALRKAEELIEEFKVPVIVEIILERVTNI
SMGTEINAVNEFEDLALVGNDAPTAISLLD

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=564, Percent_Identity=25.531914893617, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI21361361, Length=602, Percent_Identity=23.5880398671096, Blast_Score=119, Evalue=8e-27,
Organism=Escherichia coli, GI1786717, Length=590, Percent_Identity=73.8983050847458, Blast_Score=947, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=570, Percent_Identity=33.6842105263158, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1790104, Length=484, Percent_Identity=35.1239669421488, Blast_Score=271, Evalue=6e-74,
Organism=Escherichia coli, GI1787096, Length=552, Percent_Identity=24.6376811594203, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788716, Length=477, Percent_Identity=27.0440251572327, Blast_Score=142, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=512, Percent_Identity=22.8515625, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17531301, Length=512, Percent_Identity=22.8515625, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17542570, Length=559, Percent_Identity=23.4347048300537, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323755, Length=576, Percent_Identity=31.0763888888889, Blast_Score=253, Evalue=7e-68,
Organism=Saccharomyces cerevisiae, GI6320816, Length=564, Percent_Identity=24.468085106383, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321524, Length=517, Percent_Identity=21.2765957446809, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320123, Length=491, Percent_Identity=20.3665987780041, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI19922626, Length=577, Percent_Identity=26.3431542461005, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 64229; Mature: 64097

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKMRAIEAAVLVMRREGVDTAFGIPGAAINPLYSALQKVGGIDHVLARHVEGASHMAEG
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHC
YTRTKAGNIGVCIGTSGPAGTDMVTGLYSASADSIPILCITGQAPRARMHKEDFQAVDIT
CCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHCHHHCCEECHH
SIVKPVTKWATTVLEPGQVPYAFQKAFYEMRSGRPGPVLIDLPFDVQMAEIEFDIDAYQP
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEECCCCCC
LPLAKPSATRVQVEKALAMLDQAERPLLVAGGGIINADASELLVEFAELTGIPVIPTLMG
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEECCCHHHHHHHHHHHCCCCCHHHHHC
WGTIPDDHPLMVGMVGLQTSHRYGNATLLKSDVVLGVGNRWANRHTGSVDVYTEGRKFIH
CCCCCCCCCEEEEEEEECCCCCCCCEEEEECCEEEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFTPDLGIVSDAAAALTVFIEVAREWQAAGKLKNRSAWLQDCQQRKASLQ
EECCHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RKTHFDNVPVKPQRVYEEMNQVFGKDTCYVSTIGLSQIAGAQFLHVYKPRHWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCEEEEECCCCEECCCCCCC
LGWTIPAALGVVKADPNRKVVALSGDYDFQFMIEELAVGAQFKLPYIHVVVNNSYLGLIR
CCEEHHHHHEEEECCCCCEEEEEECCCCHHHHHHHHHCCCEECCCEEEEEECCCHHHHHH
QAQRGFDMDYCVQLSFDNLNAPELNGYGVDHVAVAEGLGCKALRVFEPADIAPALRKAEE
HHHHCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCCCHHHHHHHHHH
LIEEFKVPVIVEIILERVTNISMGTEINAVNEFEDLALVGNDAPTAISLLD
HHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCEEEECC
>Mature Secondary Structure 
SKMRAIEAAVLVMRREGVDTAFGIPGAAINPLYSALQKVGGIDHVLARHVEGASHMAEG
CHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHC
YTRTKAGNIGVCIGTSGPAGTDMVTGLYSASADSIPILCITGQAPRARMHKEDFQAVDIT
CCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHCHHHCCEECHH
SIVKPVTKWATTVLEPGQVPYAFQKAFYEMRSGRPGPVLIDLPFDVQMAEIEFDIDAYQP
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEECCCCCC
LPLAKPSATRVQVEKALAMLDQAERPLLVAGGGIINADASELLVEFAELTGIPVIPTLMG
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEECCCHHHHHHHHHHHCCCCCHHHHHC
WGTIPDDHPLMVGMVGLQTSHRYGNATLLKSDVVLGVGNRWANRHTGSVDVYTEGRKFIH
CCCCCCCCCEEEEEEEECCCCCCCCEEEEECCEEEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFTPDLGIVSDAAAALTVFIEVAREWQAAGKLKNRSAWLQDCQQRKASLQ
EECCHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RKTHFDNVPVKPQRVYEEMNQVFGKDTCYVSTIGLSQIAGAQFLHVYKPRHWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCEEEEECCCCEECCCCCCC
LGWTIPAALGVVKADPNRKVVALSGDYDFQFMIEELAVGAQFKLPYIHVVVNNSYLGLIR
CCEEHHHHHEEEECCCCCEEEEEECCCCHHHHHHHHHCCCEECCCEEEEEECCCHHHHHH
QAQRGFDMDYCVQLSFDNLNAPELNGYGVDHVAVAEGLGCKALRVFEPADIAPALRKAEE
HHHHCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCCCHHHHHHHHHH
LIEEFKVPVIVEIILERVTNISMGTEINAVNEFEDLALVGNDAPTAISLLD
HHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]