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The map label for this gene is rfbF [H]

Identifier: 77457737

GI number: 77457737

Start: 1697643

End: 1698416

Strand: Direct

Name: rfbF [H]

Synonym: Pfl01_1510

Alternate gene names: 77457737

Gene position: 1697643-1698416 (Clockwise)

Preceding gene: 77457736

Following gene: 77457738

Centisome position: 26.37

GC content: 59.3

Gene sequence:

>774_bases
ATGAAGGCAGTTATTTTGGCGGGTGGCCTGGGCACGCGCATCAGTGAAGAGTCGCACCTCAAGCCGAAACCGATGATCGA
GATCGGTGGCAAGCCAATTCTCTGGCACATCATGAAACAGTATTCCGCCCACGGGATTCATGACTTCGTGATTTGCCTTG
GCTACAAGGGCTACGCGATCAAGGACTTCTTCGCCAACTACTTCCTGCACACCTCCGACGTGACGTTCGACATGCGTGAA
AATCGCATGGACGTTCACCAGAACTACAGCGAACCGTGGCGCGTGACGCTGATCGACACCGGCGAGGAAACCATGACCGG
TGGTCGTCTGCGCCGCGCCGCACGCTATCTGGAAAACGAACAAGCCTTCTGCTTCACCTATGGCGACGGCGTCTCCGACC
TGAATATCAGCTCGCTGGTGGATTTCCACCTGACTCACGGCAAGTTGGCGACAGTCACCGCCGTACAACCGCCGGGCCGC
TATGGCGCGCTGGATCGTGACGGCGACCGGGTTCTCGGTTTCACGGAAAAACCGCGCGGTGACGGTGGCTGGATCAACGG
TGGTTTCTTTGTGCTCTCGCCCAAGGTTTTGCCGCTGATCGAAGGCGACGAAACCTCGTGGGAGTCCGGCCCGCTCGACG
GTCTGGCCGAGCGCGGCGAGTTGATGGCGTACCAGCACGAAGGCTTCTGGCAGCCAATGGACACCTTGCGTGACAAGAAC
CACCTCGAAGCCTTGTGGCAGAGCGGGGAGGCCCCATGGAAGCAATGGGACTGA

Upstream 100 bases:

>100_bases
GGTCATTGCCCCTGCGCCCGTACCGGCAGAAAGGTTTAGCCAGAAGGCCGCAATGCGCAAAAAGCAGCGCGCAGCCCTGT
GACGAACGAGAGGGGAGACC

Downstream 100 bases:

>100_bases
GTCCGGAATTCTGGCGCGGCAAGCGGGTTCTGGTCACCGGCCACACCGGTTTCAAGGGCAGCTGGCTGACCCTGTGGCTG
CAAAGCCTCGGCGCGCAAGT

Product: nucleotidyl transferase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKAVILAGGLGTRISEESHLKPKPMIEIGGKPILWHIMKQYSAHGIHDFVICLGYKGYAIKDFFANYFLHTSDVTFDMRE
NRMDVHQNYSEPWRVTLIDTGEETMTGGRLRRAARYLENEQAFCFTYGDGVSDLNISSLVDFHLTHGKLATVTAVQPPGR
YGALDRDGDRVLGFTEKPRGDGGWINGGFFVLSPKVLPLIEGDETSWESGPLDGLAERGELMAYQHEGFWQPMDTLRDKN
HLEALWQSGEAPWKQWD

Sequences:

>Translated_257_residues
MKAVILAGGLGTRISEESHLKPKPMIEIGGKPILWHIMKQYSAHGIHDFVICLGYKGYAIKDFFANYFLHTSDVTFDMRE
NRMDVHQNYSEPWRVTLIDTGEETMTGGRLRRAARYLENEQAFCFTYGDGVSDLNISSLVDFHLTHGKLATVTAVQPPGR
YGALDRDGDRVLGFTEKPRGDGGWINGGFFVLSPKVLPLIEGDETSWESGPLDGLAERGELMAYQHEGFWQPMDTLRDKN
HLEALWQSGEAPWKQWD
>Mature_257_residues
MKAVILAGGLGTRISEESHLKPKPMIEIGGKPILWHIMKQYSAHGIHDFVICLGYKGYAIKDFFANYFLHTSDVTFDMRE
NRMDVHQNYSEPWRVTLIDTGEETMTGGRLRRAARYLENEQAFCFTYGDGVSDLNISSLVDFHLTHGKLATVTAVQPPGR
YGALDRDGDRVLGFTEKPRGDGGWINGGFFVLSPKVLPLIEGDETSWESGPLDGLAERGELMAYQHEGFWQPMDTLRDKN
HLEALWQSGEAPWKQWD

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=246, Percent_Identity=30.0813008130081, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI11761619, Length=252, Percent_Identity=29.3650793650794, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI133931050, Length=248, Percent_Identity=32.258064516129, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6320148, Length=247, Percent_Identity=29.9595141700405, Blast_Score=102, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21355443, Length=250, Percent_Identity=28, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24644084, Length=250, Percent_Identity=28, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 29037; Mature: 29037

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVILAGGLGTRISEESHLKPKPMIEIGGKPILWHIMKQYSAHGIHDFVICLGYKGYAI
CCEEEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCCCH
KDFFANYFLHTSDVTFDMRENRMDVHQNYSEPWRVTLIDTGEETMTGGRLRRAARYLENE
HHHHHHHEEECCCEEEEHHCCCHHHHCCCCCCEEEEEEECCCHHHCCHHHHHHHHHHCCC
QAFCFTYGDGVSDLNISSLVDFHLTHGKLATVTAVQPPGRYGALDRDGDRVLGFTEKPRG
CEEEEEECCCCCCCCHHHHEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCCC
DGGWINGGFFVLSPKVLPLIEGDETSWESGPLDGLAERGELMAYQHEGFWQPMDTLRDKN
CCCEECCCEEEECCCEEEEEECCCCCCCCCCCCCHHCCCCEEEEECCCCCCCHHHHCCCH
HLEALWQSGEAPWKQWD
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKAVILAGGLGTRISEESHLKPKPMIEIGGKPILWHIMKQYSAHGIHDFVICLGYKGYAI
CCEEEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCCCH
KDFFANYFLHTSDVTFDMRENRMDVHQNYSEPWRVTLIDTGEETMTGGRLRRAARYLENE
HHHHHHHEEECCCEEEEHHCCCHHHHCCCCCCEEEEEEECCCHHHCCHHHHHHHHHHCCC
QAFCFTYGDGVSDLNISSLVDFHLTHGKLATVTAVQPPGRYGALDRDGDRVLGFTEKPRG
CEEEEEECCCCCCCCHHHHEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCCC
DGGWINGGFFVLSPKVLPLIEGDETSWESGPLDGLAERGELMAYQHEGFWQPMDTLRDKN
CCCEECCCEEEECCCEEEEEECCCCCCCCCCCCCHHCCCCEEEEECCCCCCCHHHHCCCH
HLEALWQSGEAPWKQWD
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]