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The map label for this gene is cynX [H]

Identifier: 77457613

GI number: 77457613

Start: 1558705

End: 1559889

Strand: Reverse

Name: cynX [H]

Synonym: Pfl01_1386

Alternate gene names: 77457613

Gene position: 1559889-1558705 (Counterclockwise)

Preceding gene: 77457618

Following gene: 77457612

Centisome position: 24.23

GC content: 63.46

Gene sequence:

>1185_bases
ATGGAAAACGTACGCGCCACTGCCCGCCCCGCTGTCTGGCTGATACTCGGCATCATCCTGGTCGCCCTCAACCTGCGCCC
GTCGATGGCGGCGGTCGGGCCCTTGCTCTCGGCCATTCGCGGCGATATGGCGCTGAGTTTCAGTCTCGCGGCATTGCTGA
CCCTGCTGCCGGTGATGGCGATGGGGCTGGCGATGTTTTTCGGCATGGAGGTCAGTCGGCGCCTCGGTGAACAGCGCACG
GTACTGCTGTCGTTGCTGATCATTGGCTTGGCGACGCTGTCACGGTTGTTTCTCGACTCGGCGGGCGGATTGATCGCCAG
CGCCGTGCTGGCCGGGATCGGCATTGCCCTGATCCAGGCCTTGATGCCCGCGCTGATCAAATCGCGGTTTCCCGACAACG
TGGCGCTGTGCATGGGGCTTTACGTCACTTCGATCATGGGCGGCGCGGCACTGGCCGCATCGTTCGCACCGTGGGTGTTG
ATGCAAACCGGTAACTGGCGGGCAGGGCTTGCCGTCTGGGCGGTTTTGGCACTGCTCGCCATTCCTGTGTGGTGGGCAAC
CCGTGCCACACCACCGGACACCACCCGGGCCGTCGTCAGAAAAGAATCCTTCATCACGAATTCCCGCGCCTGGTTGCTCG
CGATCTTCTTCGGTCTGGGCACCGCGTCCTACACCTGCGTGCTCGCCTGGCTGGCGCCGTACTACGTGGAAAAGGGCTGG
AACGAACAAAACGCCGGCCTGATGCTGGGTTTTCTGACGGCCATGGAGGTGATTTCCGGGCTGGTGGTGCCGGCCATCGC
CAATCGCAGCCGTGACCGACGTCTGGTGCTTGGGGCACTGCTCGGCCTGATCATCACCGGGTTCCTGGGGCTGATCCTCT
GCCCGCAGCACCTGAGCCTGTTGTGGCCCTGCCTGCTCGGACTCGGCATCGGCGGGTTGTTCCCGATGAGCCTGATCGTG
TCCCTCGATCACCTCGACGAACCACAACGTGCCGGCGGGCTGACCGCGTTCGTACAAGGTATCGGTTACCTGATTGCCGG
GCTGTCGCCGCTGATGGCCGGGGTGGTTCGCGATCGACTGGGCAGCTTCGAGTGGGCCTGGTGGTCACTGACGGCAGTGA
TGGTGCTTATGCTGCTGATGGTGCTGCGCTTCAACCCACGGCATTACGCGCAACACATGGACTAA

Upstream 100 bases:

>100_bases
GGGACCGTATCGAATTTCTCGATACCGCTCCCGACTTTCTGATTTGCCACCCCGCTCGCCTGCCCGCCTAATCGACCGCC
CCTGGTTCAAAGGTCTGACG

Downstream 100 bases:

>100_bases
TCTGGCCGACCCGTCTGGATAAAGGCTTCTGGCCATCTTTACCGGCTTTAAGTTGGCACGTCGTGTTACTAAAACTTTCT
GTCCCACAACTGACCGTGAA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; Cyanate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MENVRATARPAVWLILGIILVALNLRPSMAAVGPLLSAIRGDMALSFSLAALLTLLPVMAMGLAMFFGMEVSRRLGEQRT
VLLSLLIIGLATLSRLFLDSAGGLIASAVLAGIGIALIQALMPALIKSRFPDNVALCMGLYVTSIMGGAALAASFAPWVL
MQTGNWRAGLAVWAVLALLAIPVWWATRATPPDTTRAVVRKESFITNSRAWLLAIFFGLGTASYTCVLAWLAPYYVEKGW
NEQNAGLMLGFLTAMEVISGLVVPAIANRSRDRRLVLGALLGLIITGFLGLILCPQHLSLLWPCLLGLGIGGLFPMSLIV
SLDHLDEPQRAGGLTAFVQGIGYLIAGLSPLMAGVVRDRLGSFEWAWWSLTAVMVLMLLMVLRFNPRHYAQHMD

Sequences:

>Translated_394_residues
MENVRATARPAVWLILGIILVALNLRPSMAAVGPLLSAIRGDMALSFSLAALLTLLPVMAMGLAMFFGMEVSRRLGEQRT
VLLSLLIIGLATLSRLFLDSAGGLIASAVLAGIGIALIQALMPALIKSRFPDNVALCMGLYVTSIMGGAALAASFAPWVL
MQTGNWRAGLAVWAVLALLAIPVWWATRATPPDTTRAVVRKESFITNSRAWLLAIFFGLGTASYTCVLAWLAPYYVEKGW
NEQNAGLMLGFLTAMEVISGLVVPAIANRSRDRRLVLGALLGLIITGFLGLILCPQHLSLLWPCLLGLGIGGLFPMSLIV
SLDHLDEPQRAGGLTAFVQGIGYLIAGLSPLMAGVVRDRLGSFEWAWWSLTAVMVLMLLMVLRFNPRHYAQHMD
>Mature_394_residues
MENVRATARPAVWLILGIILVALNLRPSMAAVGPLLSAIRGDMALSFSLAALLTLLPVMAMGLAMFFGMEVSRRLGEQRT
VLLSLLIIGLATLSRLFLDSAGGLIASAVLAGIGIALIQALMPALIKSRFPDNVALCMGLYVTSIMGGAALAASFAPWVL
MQTGNWRAGLAVWAVLALLAIPVWWATRATPPDTTRAVVRKESFITNSRAWLLAIFFGLGTASYTCVLAWLAPYYVEKGW
NEQNAGLMLGFLTAMEVISGLVVPAIANRSRDRRLVLGALLGLIITGFLGLILCPQHLSLLWPCLLGLGIGGLFPMSLIV
SLDHLDEPQRAGGLTAFVQGIGYLIAGLSPLMAGVVRDRLGSFEWAWWSLTAVMVLMLLMVLRFNPRHYAQHMD

Specific function: This protein is part of an active transport system that transports exogenous cyanate into E.coli cells [H]

COG id: COG2807

COG function: function code P; Cyanate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Cyanate porter (TC 2.A.1.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786536, Length=384, Percent_Identity=39.5833333333333, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1788092, Length=383, Percent_Identity=28.4595300261097, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004747
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42364; Mature: 42364

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENVRATARPAVWLILGIILVALNLRPSMAAVGPLLSAIRGDMALSFSLAALLTLLPVMA
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGLAMFFGMEVSRRLGEQRTVLLSLLIIGLATLSRLFLDSAGGLIASAVLAGIGIALIQA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LMPALIKSRFPDNVALCMGLYVTSIMGGAALAASFAPWVLMQTGNWRAGLAVWAVLALLA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHH
IPVWWATRATPPDTTRAVVRKESFITNSRAWLLAIFFGLGTASYTCVLAWLAPYYVEKGW
HHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCC
NEQNAGLMLGFLTAMEVISGLVVPAIANRSRDRRLVLGALLGLIITGFLGLILCPQHLSL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWPCLLGLGIGGLFPMSLIVSLDHLDEPQRAGGLTAFVQGIGYLIAGLSPLMAGVVRDRL
HHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSFEWAWWSLTAVMVLMLLMVLRFNPRHYAQHMD
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MENVRATARPAVWLILGIILVALNLRPSMAAVGPLLSAIRGDMALSFSLAALLTLLPVMA
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGLAMFFGMEVSRRLGEQRTVLLSLLIIGLATLSRLFLDSAGGLIASAVLAGIGIALIQA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LMPALIKSRFPDNVALCMGLYVTSIMGGAALAASFAPWVLMQTGNWRAGLAVWAVLALLA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHH
IPVWWATRATPPDTTRAVVRKESFITNSRAWLLAIFFGLGTASYTCVLAWLAPYYVEKGW
HHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCC
NEQNAGLMLGFLTAMEVISGLVVPAIANRSRDRRLVLGALLGLIITGFLGLILCPQHLSL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWPCLLGLGIGGLFPMSLIVSLDHLDEPQRAGGLTAFVQGIGYLIAGLSPLMAGVVRDRL
HHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSFEWAWWSLTAVMVLMLLMVLRFNPRHYAQHMD
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; Cyanate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + Cyanate [Periplasm] = Proton [Cytoplasm] + Cyanate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3049588; 9278503 [H]