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The map label for this gene is ygfZ [H]

Identifier: 77457584

GI number: 77457584

Start: 1532217

End: 1533158

Strand: Reverse

Name: ygfZ [H]

Synonym: Pfl01_1357

Alternate gene names: 77457584

Gene position: 1533158-1532217 (Counterclockwise)

Preceding gene: 77457587

Following gene: 77457583

Centisome position: 23.81

GC content: 61.89

Gene sequence:

>942_bases
ATGGCCGATTCTGCTTTTTTCTGCACCCTGTCTCATGAAGGCGTTCTCGCGGTTCGCGGCGCGGATGCCGGCAAATTCCT
GCAAGGCCAGCTGACCTGCAACATCAATTACCTGAGTGAAACCCAGGCCAGCCTTGGTGCCCGTTGCACACAGAAAGGCC
GGATGCAGTCGAGTTTCCGCATCGTGCTTGAAGGCGACGGCGTGCTGCTGGCGATGGCCGGCGCGCTGCTGGAACCGCAA
CTGGCGGATCTGAAGAAGTACGCGGTGTTCTCCAAATCGAAACTGACCGACGAAAGCGCCTCCTGGGTGCGCTTCGGTCT
GGATCATGGCGATGCGGCCCTGAGCAGCCTGGGTCTGGAACTGCCGGCAGAAACAGACAGCGTGGCCCGCCACGAAGGGC
TGATCGCGATTCGCGTCTCCCCTAATCGTGCCGAACTCTGGGTGCCTGCCGATCAGGCCGACACCGTCAAAGGCAAGCTG
TGCGCGCAATTGACCGAAGCCGAACTCAATCAATGGCTGCTGGGCCAGATCCGCGCCGGTATCGGCCAGGTCATGCCGAG
CACCCGTGAGCTGTTCATCCCGCAGATGCTCAATCTGCAGGCCGTCGGCGGTGTGAGCTTCAAGAAAGGCTGCTACACCG
GCCAGGAAATCGTCGCGCGCATGCAGTACCTGGGCAAACTCAAACGTCGCCTGTATCGCGTGAAACTGGATGCCGCTGAG
TTGCCGGAGCCCGGCACCGCGCTGTTCGCCCCGAGCCACGGCAGTTCGATCGGCGAAGTGGTGCTGGCCGCCCGTACCGA
AAAGAATATTGAACTGCTGGCGGTGTTGCAGGCCGAAGCTGCCGAAGCGGGCGACTTGCATCTGGGCTCGGCCGAAGGTC
CGGCGCTGCACTTGCTCGACCTGCCATACGAACTGGATCGCGACCGCGAAATCCAGCGTTGA

Upstream 100 bases:

>100_bases
ACAGTCGGCTTTGGTCGGGCGCCATCGATCCTTTATCAACTACCCATTTCAACACCACCCCCTTATGATGTGCCCCAGTC
TATTTACCCTGCGATGACCC

Downstream 100 bases:

>100_bases
TCGCAGCATTTGTTGCAACACCCTAGAGAAACGACATGAGTGAGCTGGCGGATAAGGTCCAACAGGATTTGGTTGAGGCC
ATCGATAACGATGACCTGGT

Product: glycine cleavage T protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MADSAFFCTLSHEGVLAVRGADAGKFLQGQLTCNINYLSETQASLGARCTQKGRMQSSFRIVLEGDGVLLAMAGALLEPQ
LADLKKYAVFSKSKLTDESASWVRFGLDHGDAALSSLGLELPAETDSVARHEGLIAIRVSPNRAELWVPADQADTVKGKL
CAQLTEAELNQWLLGQIRAGIGQVMPSTRELFIPQMLNLQAVGGVSFKKGCYTGQEIVARMQYLGKLKRRLYRVKLDAAE
LPEPGTALFAPSHGSSIGEVVLAARTEKNIELLAVLQAEAAEAGDLHLGSAEGPALHLLDLPYELDRDREIQR

Sequences:

>Translated_313_residues
MADSAFFCTLSHEGVLAVRGADAGKFLQGQLTCNINYLSETQASLGARCTQKGRMQSSFRIVLEGDGVLLAMAGALLEPQ
LADLKKYAVFSKSKLTDESASWVRFGLDHGDAALSSLGLELPAETDSVARHEGLIAIRVSPNRAELWVPADQADTVKGKL
CAQLTEAELNQWLLGQIRAGIGQVMPSTRELFIPQMLNLQAVGGVSFKKGCYTGQEIVARMQYLGKLKRRLYRVKLDAAE
LPEPGTALFAPSHGSSIGEVVLAARTEKNIELLAVLQAEAAEAGDLHLGSAEGPALHLLDLPYELDRDREIQR
>Mature_312_residues
ADSAFFCTLSHEGVLAVRGADAGKFLQGQLTCNINYLSETQASLGARCTQKGRMQSSFRIVLEGDGVLLAMAGALLEPQL
ADLKKYAVFSKSKLTDESASWVRFGLDHGDAALSSLGLELPAETDSVARHEGLIAIRVSPNRAELWVPADQADTVKGKLC
AQLTEAELNQWLLGQIRAGIGQVMPSTRELFIPQMLNLQAVGGVSFKKGCYTGQEIVARMQYLGKLKRRLYRVKLDAAEL
PEPGTALFAPSHGSSIGEVVLAARTEKNIELLAVLQAEAAEAGDLHLGSAEGPALHLLDLPYELDRDREIQR

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=313, Percent_Identity=35.4632587859425, Blast_Score=155, Evalue=4e-39,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 33798; Mature: 33667

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSAFFCTLSHEGVLAVRGADAGKFLQGQLTCNINYLSETQASLGARCTQKGRMQSSFR
CCCCEEEEEECCCCEEEEECCCCCCEEEEEEEEEEEEHHHHHHHHHHHHHHCCCCCCCEE
IVLEGDGVLLAMAGALLEPQLADLKKYAVFSKSKLTDESASWVRFGLDHGDAALSSLGLE
EEEECCCEEEEEHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHCCCC
LPAETDSVARHEGLIAIRVSPNRAELWVPADQADTVKGKLCAQLTEAELNQWLLGQIRAG
CCCCCCCHHHHCCEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IGQVMPSTRELFIPQMLNLQAVGGVSFKKGCYTGQEIVARMQYLGKLKRRLYRVKLDAAE
HHHHCCCHHHHHCHHHHCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECHHH
LPEPGTALFAPSHGSSIGEVVLAARTEKNIELLAVLQAEAAEAGDLHLGSAEGPALHLLD
CCCCCCEEEECCCCCCHHHEEEEECCCCCEEEEEEEEHHCCCCCCEEECCCCCCEEEEEE
LPYELDRDREIQR
CCCCCCCCCCCCC
>Mature Secondary Structure 
ADSAFFCTLSHEGVLAVRGADAGKFLQGQLTCNINYLSETQASLGARCTQKGRMQSSFR
CCCEEEEEECCCCEEEEECCCCCCEEEEEEEEEEEEHHHHHHHHHHHHHHCCCCCCCEE
IVLEGDGVLLAMAGALLEPQLADLKKYAVFSKSKLTDESASWVRFGLDHGDAALSSLGLE
EEEECCCEEEEEHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHCCCC
LPAETDSVARHEGLIAIRVSPNRAELWVPADQADTVKGKLCAQLTEAELNQWLLGQIRAG
CCCCCCCHHHHCCEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IGQVMPSTRELFIPQMLNLQAVGGVSFKKGCYTGQEIVARMQYLGKLKRRLYRVKLDAAE
HHHHCCCHHHHHCHHHHCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECHHH
LPEPGTALFAPSHGSSIGEVVLAARTEKNIELLAVLQAEAAEAGDLHLGSAEGPALHLLD
CCCCCCEEEECCCCCCHHHEEEEECCCCCEEEEEEEEHHCCCCCCEEECCCCCCEEEEEE
LPYELDRDREIQR
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA