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The map label for this gene is oprF [H]

Identifier: 77457293

GI number: 77457293

Start: 1234871

End: 1235665

Strand: Reverse

Name: oprF [H]

Synonym: Pfl01_1066

Alternate gene names: 77457293

Gene position: 1235665-1234871 (Counterclockwise)

Preceding gene: 77457294

Following gene: 77457277

Centisome position: 19.19

GC content: 60.75

Gene sequence:

>795_bases
ATGCGTAAACAACTGATGATCCCCGCTCTCCTGGCCGCAAGCGTTGCCCTGGCTGCCTGCTCCACGCCGCCCAACCCGAA
TCTGGAACAGGCACGCACCAACTACGCCGGCCTGCAGGCCAACCCGCAGGCAAGCAAGGTCGCAGCGCTGGAAACCAAGG
ACGCCAGCGACTATCTGGACAAGGCCGACAAGGCTTATCTGGATAAAGAGGACGCCGCCAAGGTCGATCAACTGGCTTAT
CTGACCAACCAGCGCGTGGAAGTGGCCAAGCAGACCATCGCCCTGCGTACCGCTGAAAACAACCTGAAGAACGCCGCCGC
CCAACGTGCCCAGGCCCGTCTGGACGCTCGCGATCAGCAGATCAAGCAGCTGCAGGACAGCCTCAACGCCAAGCAGACCG
ATCGCGGTACGCTGGTGACTTTCGGTGATGTGCTGTTCGCCACCAACAAGGCCGACCTGAAATCCAGCGGTCTGGTGAAC
ATCAACAAACTGGCACAATTCCTCCAGGAAAACCCGGATCGCAAAGTGATCGTCGAAGGCTATACCGACAGCACCGGTTC
GGCGAGCTACAACCAGTCGCTGTCCGAGCGTCGCGCGACCTCGGTGCAGGTTGCGCTGATCAAGATGGGTGTAGACCCTT
CGCGCATCGTGGTGCAGGGCTATGGCAAGGAATACCCGGTTGCGGATAACGGCAGCGTGTCGGGCCGTGCCATGAACCGT
CGGGTGGAAGTGACCATTTCCAACGACAACCAGCCAGTGATGCCGCGTTCGGCCGTGAGCTCGACCGCGCACTGA

Upstream 100 bases:

>100_bases
GGTGTTCAGGAACTGCGTCAGGAAAGTCAGCGCCCAGTGCAGTAAGCGCGCATCCCGACCGCAAGGCTGAAACTCTTATC
GATATAAAGGACGAACGATT

Downstream 100 bases:

>100_bases
TTCGCTGAGCAGTAAATGAAAAGCCCCGCCTGAGTGATCAGGCGGGGCTTTTTGTTTGGATGCTCGGCTCGGAATGTGTG
GGGGATCAGAGATCGCAGCC

Product: OmpA/MotB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRKQLMIPALLAASVALAACSTPPNPNLEQARTNYAGLQANPQASKVAALETKDASDYLDKADKAYLDKEDAAKVDQLAY
LTNQRVEVAKQTIALRTAENNLKNAAAQRAQARLDARDQQIKQLQDSLNAKQTDRGTLVTFGDVLFATNKADLKSSGLVN
INKLAQFLQENPDRKVIVEGYTDSTGSASYNQSLSERRATSVQVALIKMGVDPSRIVVQGYGKEYPVADNGSVSGRAMNR
RVEVTISNDNQPVMPRSAVSSTAH

Sequences:

>Translated_264_residues
MRKQLMIPALLAASVALAACSTPPNPNLEQARTNYAGLQANPQASKVAALETKDASDYLDKADKAYLDKEDAAKVDQLAY
LTNQRVEVAKQTIALRTAENNLKNAAAQRAQARLDARDQQIKQLQDSLNAKQTDRGTLVTFGDVLFATNKADLKSSGLVN
INKLAQFLQENPDRKVIVEGYTDSTGSASYNQSLSERRATSVQVALIKMGVDPSRIVVQGYGKEYPVADNGSVSGRAMNR
RVEVTISNDNQPVMPRSAVSSTAH
>Mature_264_residues
MRKQLMIPALLAASVALAACSTPPNPNLEQARTNYAGLQANPQASKVAALETKDASDYLDKADKAYLDKEDAAKVDQLAY
LTNQRVEVAKQTIALRTAENNLKNAAAQRAQARLDARDQQIKQLQDSLNAKQTDRGTLVTFGDVLFATNKADLKSSGLVN
INKLAQFLQENPDRKVIVEGYTDSTGSASYNQSLSERRATSVQVALIKMGVDPSRIVVQGYGKEYPVADNGSVSGRAMNR
RVEVTISNDNQPVMPRSAVSSTAH

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=105, Percent_Identity=47.6190476190476, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787191, Length=126, Percent_Identity=40.4761904761905, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1786962, Length=98, Percent_Identity=38.7755102040816, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788957, Length=105, Percent_Identity=39.0476190476191, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 28579; Mature: 28579

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKQLMIPALLAASVALAACSTPPNPNLEQARTNYAGLQANPQASKVAALETKDASDYLD
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHH
KADKAYLDKEDAAKVDQLAYLTNQRVEVAKQTIALRTAENNLKNAAAQRAQARLDARDQQ
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
IKQLQDSLNAKQTDRGTLVTFGDVLFATNKADLKSSGLVNINKLAQFLQENPDRKVIVEG
HHHHHHHHCCCCCCCCCEEEECCEEEECCCCCCCCCCCEEHHHHHHHHHCCCCCEEEEEC
YTDSTGSASYNQSLSERRATSVQVALIKMGVDPSRIVVQGYGKEYPVADNGSVSGRAMNR
CCCCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEECE
RVEVTISNDNQPVMPRSAVSSTAH
EEEEEECCCCCCCCCHHHHHCCCC
>Mature Secondary Structure
MRKQLMIPALLAASVALAACSTPPNPNLEQARTNYAGLQANPQASKVAALETKDASDYLD
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHH
KADKAYLDKEDAAKVDQLAYLTNQRVEVAKQTIALRTAENNLKNAAAQRAQARLDARDQQ
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
IKQLQDSLNAKQTDRGTLVTFGDVLFATNKADLKSSGLVNINKLAQFLQENPDRKVIVEG
HHHHHHHHCCCCCCCCCEEEECCEEEECCCCCCCCCCCEEHHHHHHHHHCCCCCEEEEEC
YTDSTGSASYNQSLSERRATSVQVALIKMGVDPSRIVVQGYGKEYPVADNGSVSGRAMNR
CCCCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEECE
RVEVTISNDNQPVMPRSAVSSTAH
EEEEEECCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]