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The map label for this gene is puuP [H]

Identifier: 77457270

GI number: 77457270

Start: 1208100

End: 1209584

Strand: Reverse

Name: puuP [H]

Synonym: Pfl01_1043

Alternate gene names: 77457270

Gene position: 1209584-1208100 (Counterclockwise)

Preceding gene: 77457271

Following gene: 77457267

Centisome position: 18.79

GC content: 61.48

Gene sequence:

>1485_bases
ATGAGTGCATTACCCGATCTCTCAGCCGCCGTTGCCGACAGCGATGCCGAACAACTGCGCCAACTGGGTTACACCTCCAA
CTTCAACCGCAGCATGAGCCTGTGGGAAAACTTCGCGTTGGGCTTCACTTATCTGTCGCCGGTCGTAGGGGTTTATACCC
TGTTCGGCCTGTGCCTGGCCGCTGGCGGGCCGCCGATGTTCTGGGCCTATTTGCTGGTCGGTTGCGGTCAATTGCTGGTG
TGCCTGATCTTCGGCGAAGTGGTGTCGCAGTTCCCGATTTCCGGCGGTGTCTATCCTTGGGCGCGTCGCTTGGTGGGCAA
GAAATGGGCATGGATGGTCGGCTGGATCTACTCCATAGCCCTGTGCGTGACCATCGCCGCCGTGGCAGTCGGTGCCGGCC
CGTACCTCGCCGCCATGCTGGGTTTTGAGCCAAGCAACAACACCAACATCGTCATCGCCCTGGTACTGACCCTGTTCGCC
ACACTGGTCAACCTCAGCGGCACCAAAGTGCTGGCGCGGATCGCCATGTTCGGCTTCCTCTGCGAACTGGTGGGCGCGGT
GATCGTCGGCGTGTACCTGCTGATTTTCGAGCGTCACCAGCCGATCAGCGTGCTGTTCAACACCTTCGATATCAGCGTCG
ACGGCTCCTACCTGCCGGCCTTCCTCACCGCTTCGCTGGCCGGGATGTTCCTCTACTACGGCTTCGAGGCCTGCGGCGAC
GTCGCCGAAGAAACCCCGAACCCGAGCGCAAAAATCCCGGTGGCCATGCGCATGACCATCTACATCGGCGGCATCGCGGC
AATGTTCGCTTGCCTGGCGCTGATCCTCGCTGTGCCGGACATGCAGGCCGTGATCAACGGCACCGACAAGGATCCGGTCA
CCACCATCCTCAACAACGCCTTCGGCCCGGTGGGTTCGAAAGTGGTGATGGGCGTGGTAATGATTTCCTTCATCTCCTGC
GTCATCAGCCTGCAAGCGGCGGCGAGTCGTCTGCTGTACTCCTACGCTCGCGATGAGATGGTCATCGGCAGCCGACTGCT
GAAAAAGATTTCTCCTACGACCCAGGTTCCAGTTGCCGCCCTGTTCGTGTCCGGCGTCCTGCCCGGCCTGATCATCGCCC
TCGGATTTTTCCTGCAAGACGCCGTGGCGACCATCGTCAGCTTCGCCGCCATCGGCATCTACCTCGCGTTCCAGATGATC
GTCCTCGCCGCCCTGTACGCCCGCAGCAAAGGCTGGAAACCCAGCGGCAAATTCACCCTCGGCGCCTGGGGCTGGCCGGT
GAACATCGGCGCGCTGGTATACGGCGTCGGCGCAATCATCAACATGGCCTGGCCACGCACCCCGGATGCGGCGTGGTACA
TCAACTACGCGATGGTGCTGAGCACGGCGATCGTGATTGGGCTGGGGCTGGTTTATATGTGGATTGGCAAGCCGTATGAC
CATGGGAAGGCGCCGGCTGGGGATGCGTGGAAGGTGGGTCGGTAA

Upstream 100 bases:

>100_bases
AGGCATTCAAAACAATAACTACCGATCCGGGCGGCGCTCACCAAGCCCCGCCACGGCTTCGGCCATCCGAAATCTGCCGA
TTTCACGGAGCAAAACACAC

Downstream 100 bases:

>100_bases
CTCTCCTCCACCAAAATAAAGTCCCGGTTCTCAAATCACGGATCGGGACTTTGTTTGACTGCTATAGCAACTGAGGTTTA
GAAAGGATTTCGAATTCATT

Product: amino acid permease-associated protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSALPDLSAAVADSDAEQLRQLGYTSNFNRSMSLWENFALGFTYLSPVVGVYTLFGLCLAAGGPPMFWAYLLVGCGQLLV
CLIFGEVVSQFPISGGVYPWARRLVGKKWAWMVGWIYSIALCVTIAAVAVGAGPYLAAMLGFEPSNNTNIVIALVLTLFA
TLVNLSGTKVLARIAMFGFLCELVGAVIVGVYLLIFERHQPISVLFNTFDISVDGSYLPAFLTASLAGMFLYYGFEACGD
VAEETPNPSAKIPVAMRMTIYIGGIAAMFACLALILAVPDMQAVINGTDKDPVTTILNNAFGPVGSKVVMGVVMISFISC
VISLQAAASRLLYSYARDEMVIGSRLLKKISPTTQVPVAALFVSGVLPGLIIALGFFLQDAVATIVSFAAIGIYLAFQMI
VLAALYARSKGWKPSGKFTLGAWGWPVNIGALVYGVGAIINMAWPRTPDAAWYINYAMVLSTAIVIGLGLVYMWIGKPYD
HGKAPAGDAWKVGR

Sequences:

>Translated_494_residues
MSALPDLSAAVADSDAEQLRQLGYTSNFNRSMSLWENFALGFTYLSPVVGVYTLFGLCLAAGGPPMFWAYLLVGCGQLLV
CLIFGEVVSQFPISGGVYPWARRLVGKKWAWMVGWIYSIALCVTIAAVAVGAGPYLAAMLGFEPSNNTNIVIALVLTLFA
TLVNLSGTKVLARIAMFGFLCELVGAVIVGVYLLIFERHQPISVLFNTFDISVDGSYLPAFLTASLAGMFLYYGFEACGD
VAEETPNPSAKIPVAMRMTIYIGGIAAMFACLALILAVPDMQAVINGTDKDPVTTILNNAFGPVGSKVVMGVVMISFISC
VISLQAAASRLLYSYARDEMVIGSRLLKKISPTTQVPVAALFVSGVLPGLIIALGFFLQDAVATIVSFAAIGIYLAFQMI
VLAALYARSKGWKPSGKFTLGAWGWPVNIGALVYGVGAIINMAWPRTPDAAWYINYAMVLSTAIVIGLGLVYMWIGKPYD
HGKAPAGDAWKVGR
>Mature_493_residues
SALPDLSAAVADSDAEQLRQLGYTSNFNRSMSLWENFALGFTYLSPVVGVYTLFGLCLAAGGPPMFWAYLLVGCGQLLVC
LIFGEVVSQFPISGGVYPWARRLVGKKWAWMVGWIYSIALCVTIAAVAVGAGPYLAAMLGFEPSNNTNIVIALVLTLFAT
LVNLSGTKVLARIAMFGFLCELVGAVIVGVYLLIFERHQPISVLFNTFDISVDGSYLPAFLTASLAGMFLYYGFEACGDV
AEETPNPSAKIPVAMRMTIYIGGIAAMFACLALILAVPDMQAVINGTDKDPVTTILNNAFGPVGSKVVMGVVMISFISCV
ISLQAAASRLLYSYARDEMVIGSRLLKKISPTTQVPVAALFVSGVLPGLIIALGFFLQDAVATIVSFAAIGIYLAFQMIV
LAALYARSKGWKPSGKFTLGAWGWPVNIGALVYGVGAIINMAWPRTPDAAWYINYAMVLSTAIVIGLGLVYMWIGKPYDH
GKAPAGDAWKVGR

Specific function: Imports putrescine [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI87081869, Length=421, Percent_Identity=23.7529691211401, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082023, Length=398, Percent_Identity=24.8743718592965, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17532491, Length=378, Percent_Identity=26.4550264550265, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17531343, Length=390, Percent_Identity=25.1282051282051, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17533459, Length=398, Percent_Identity=23.3668341708543, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321361, Length=495, Percent_Identity=24.040404040404, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6322674, Length=504, Percent_Identity=23.015873015873, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319991, Length=508, Percent_Identity=21.0629921259843, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24667468, Length=412, Percent_Identity=25.4854368932039, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 52734; Mature: 52603

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCC
>Mature Secondary Structure 
SALPDLSAAVADSDAEQLRQLGYTSNFNRSMSLWENFALGFTYLSPVVGVYTLFGLCLA
CCCCCCHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]