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The map label for this gene is fruB(HI) [H]

Identifier: 77457231

GI number: 77457231

Start: 1168679

End: 1171192

Strand: Reverse

Name: fruB(HI) [H]

Synonym: Pfl01_1004

Alternate gene names: 77457231

Gene position: 1171192-1168679 (Counterclockwise)

Preceding gene: 77457232

Following gene: 77457230

Centisome position: 18.19

GC content: 65.39

Gene sequence:

>2514_bases
ATGCACAACAACAATAAAGAGCTGACTTTAAGCGCCCCGCTCAGCGGCCCGGTGCTCACGCTCGCCAAAGTCCCGGACGC
GGTGTTCGCCAGCGGCGCGATGGGCGACGGCATTGCCATTGATCCGCTGAACGACACCCTGTATTCGCCCTGCGCCGGGG
TGGTGATCCACGTCGCCCGCACCGGCCATGCGCTGACCGTGCGCGCCGACAACGGTGCCGAGCTGTTGCTGCACCTCGGT
CTGGACACGGTGGAGTTGCAGGGCGAAGGCTTCTCGATGCTGGTCAAGGAAGGCGCGCGGGTCAGCAATGGCCAGCCGCT
GCTGCGCTATGACCTGGACAAGGTCGGCCGCCAGTGCAAAAGCCTGGTCAGCCTGCTGATCCTGACCAACAGCCAGGATT
TCCAGGCGCGCCCCATCACCCTGAAAACGGTGAAGGTCGGCGAACCGCTGCTGCACATCGTCGCTCGTCACAGCGCAGCG
GCGAATACAGAAGAACTCGGCGGCCCGCAAGTTCACGGTCACGTAAAGGTCGCCCATCGCGGCGGTCTGCATGCGCGCCC
GGCGGCGTTGATCCGCCAGACCGCGCAAGGGTTCAAGAGCCAGTCGCAGCTGCATTTCGCCGGCAAATCGGCACCGTGCA
ACAGCCTCATCGGTTTGATGGGCCTGGCGATTGGCGAACAGGACGAAGTGCAGGTCAGTTGCCAGGGTCCGGACGCACAG
GCTGCGTTGCAAGCCTTGCTCGTCGCGCTGGCAACAGCCCTGCCGGAGGATCATCACGCCGCTGCACCGCTTGCCGCCAC
TCCACGCAATCGCCCGGCCGAGGCCGGCGTGTTGCACGGCGTGTGCGCCGCACCGGGTCTGGTCGGCGGGCCGCTGTTTC
GCCTGAACGCGATCAGCCTGCCAGCCGACAGCGGCAATCATCAGCCCGAGCAACAACTACAGATTCTCGACACGGCGCTG
AATCAGGTTCGCAGCGAAATCGACGGCACCCTCGCCCAGGCGAAAAAACAGCGCAATGCCGACGAAGAAGCCATCTTCGC
TGCGCACCTGGCCTTGCTGGAAGACCCGGCCCTGCTCGACGCCGCCCGGCAATCGATTGAAACCGGCACGGCGGCGACTC
ACGCCTGGAGCCAGTCCATCGACGCGCAATGCCAAGTGCTGCAAAACACCGGCAGCCCGCTGCTGGCCGAACGCGCCAAC
GATCTGCGCGACCTCAAGCAACGGGTGCTGCGCGCCCTGCTCGGCGAAGCCTGGCATTACGACGTGCCGGCCGGCGCCAT
CGTCGCCGCTCACGAACTGACGCCTTCGGATTTGCTGCAACTGAGCGCGCAAGGCGTCGCCGGTTTGTGCATGGCCGAAG
GTGGCGCGACTTCCCACGTGGCGATTCTGGCCCGGGGCAAAGGCCTGCCGTGCATGGTCGCACTGGGCTCGGTTTTGCTG
GATCAAACTCAAGGTCAATCAGTGGTTCTCGATGCCGACGGCGGTCGCCTCGAACTGACGCCGAATGCCGAGCGTCTGGC
CGAAGTCCAACAAGCACAGATCGACCGCCAACAACGGCGCGACGCCCAGCAAGCCCAGGCCCATCTGCCAGCCGAAACCC
GCAACGGCGTGGCCATCGAAGTAGTCGCCAACGTCGCCTCCAGCCATGAAGCGGCGGATGCATTTGCCAACGGCGCCGAC
GGCGTCGGCCTGTTGCGCACCGAGTTCCTGTTCGTCGATCGCCACACTGCGCCGGACGTCGAAGAACAACGCGCCGCCTA
TCAAGCTGTGATCGATGCCATGGGCGACAAGTCGGTGATCATCCGCACCATCGACGTCGGCGGCGACAAACAACTCGACT
ATCTGCCGCTGCCCGTGGAAGCCAACCCGGTACTCGGCCTGCGCGGCATTCGCCTGGCCCAGGCCCGCCCGGAAATCCTC
GACCAGCAACTGCGCGCACTGCTGCAAGTCAGCCCGTTGCAGCGCTGCCGGATCCTGCTGCCGATGGTCACCGAAGTCGA
CGAACTGCTGCACATCCGCCAGCGGGTCGATGCGCTGTGTCTGGAACTGGGCATCAATCAACGTCCGGAAATCGGCGTGA
TGATCGAAGTCCCGGCCGCTGCATTGCAGGCTGAACAACTGGCCGAGCACGCCGACTTCCTGTCCATCGGCACCAACGAC
CTGTCGCAATACACCCTGGCCATGGACCGCGATCACGCCGGCCTCGCCGCACGGGTCGATGCCTTGCACCCGGCGCTGCT
GCGCCTGATCGCCATGACCTGCGAAGGCGCGGCGGTGCATAAACGCTGGGTCGGCGTGTGCGGCGCCCTCGCCTCGGACC
CGCTGGCGACGCCGGTGTTGATCGGCCTGGGCGTGAGCGAACTGTCGGTGAGCCCGGTACAGATCGGCGAAATCAAGGAC
CGCGTGCGCCAGCTGCACGAAGCCGAATGCCAACGCCTCGCCCGGGACCTGCTCAAGCTGAGCAGCGCCGCCGCGGTGCG
TCATGCCTGTCATCAACATTGGCCTCTGCGCTAA

Upstream 100 bases:

>100_bases
CCGTGGCCCGTGGCATGGACCCGGATCAGCCGCGACACCTGAGCAAAGTCACCCGTACGCACTGAGTCCGAACCGAACGT
TTTCCTGATGAGACCGAGCC

Downstream 100 bases:

>100_bases
CAAAAACAACAAGGACAAACGCCATGTACCAACTCTTCATCGAAGGCCTGCAACGCCTCGGCCGTGCGCTGATGCTGCCG
ATCGCGATCTTGCCGATCGC

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: MTP; Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system enzyme I; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; EIII-Fru; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 837; Mature: 837

Protein sequence:

>837_residues
MHNNNKELTLSAPLSGPVLTLAKVPDAVFASGAMGDGIAIDPLNDTLYSPCAGVVIHVARTGHALTVRADNGAELLLHLG
LDTVELQGEGFSMLVKEGARVSNGQPLLRYDLDKVGRQCKSLVSLLILTNSQDFQARPITLKTVKVGEPLLHIVARHSAA
ANTEELGGPQVHGHVKVAHRGGLHARPAALIRQTAQGFKSQSQLHFAGKSAPCNSLIGLMGLAIGEQDEVQVSCQGPDAQ
AALQALLVALATALPEDHHAAAPLAATPRNRPAEAGVLHGVCAAPGLVGGPLFRLNAISLPADSGNHQPEQQLQILDTAL
NQVRSEIDGTLAQAKKQRNADEEAIFAAHLALLEDPALLDAARQSIETGTAATHAWSQSIDAQCQVLQNTGSPLLAERAN
DLRDLKQRVLRALLGEAWHYDVPAGAIVAAHELTPSDLLQLSAQGVAGLCMAEGGATSHVAILARGKGLPCMVALGSVLL
DQTQGQSVVLDADGGRLELTPNAERLAEVQQAQIDRQQRRDAQQAQAHLPAETRNGVAIEVVANVASSHEAADAFANGAD
GVGLLRTEFLFVDRHTAPDVEEQRAAYQAVIDAMGDKSVIIRTIDVGGDKQLDYLPLPVEANPVLGLRGIRLAQARPEIL
DQQLRALLQVSPLQRCRILLPMVTEVDELLHIRQRVDALCLELGINQRPEIGVMIEVPAAALQAEQLAEHADFLSIGTND
LSQYTLAMDRDHAGLAARVDALHPALLRLIAMTCEGAAVHKRWVGVCGALASDPLATPVLIGLGVSELSVSPVQIGEIKD
RVRQLHEAECQRLARDLLKLSSAAAVRHACHQHWPLR

Sequences:

>Translated_837_residues
MHNNNKELTLSAPLSGPVLTLAKVPDAVFASGAMGDGIAIDPLNDTLYSPCAGVVIHVARTGHALTVRADNGAELLLHLG
LDTVELQGEGFSMLVKEGARVSNGQPLLRYDLDKVGRQCKSLVSLLILTNSQDFQARPITLKTVKVGEPLLHIVARHSAA
ANTEELGGPQVHGHVKVAHRGGLHARPAALIRQTAQGFKSQSQLHFAGKSAPCNSLIGLMGLAIGEQDEVQVSCQGPDAQ
AALQALLVALATALPEDHHAAAPLAATPRNRPAEAGVLHGVCAAPGLVGGPLFRLNAISLPADSGNHQPEQQLQILDTAL
NQVRSEIDGTLAQAKKQRNADEEAIFAAHLALLEDPALLDAARQSIETGTAATHAWSQSIDAQCQVLQNTGSPLLAERAN
DLRDLKQRVLRALLGEAWHYDVPAGAIVAAHELTPSDLLQLSAQGVAGLCMAEGGATSHVAILARGKGLPCMVALGSVLL
DQTQGQSVVLDADGGRLELTPNAERLAEVQQAQIDRQQRRDAQQAQAHLPAETRNGVAIEVVANVASSHEAADAFANGAD
GVGLLRTEFLFVDRHTAPDVEEQRAAYQAVIDAMGDKSVIIRTIDVGGDKQLDYLPLPVEANPVLGLRGIRLAQARPEIL
DQQLRALLQVSPLQRCRILLPMVTEVDELLHIRQRVDALCLELGINQRPEIGVMIEVPAAALQAEQLAEHADFLSIGTND
LSQYTLAMDRDHAGLAARVDALHPALLRLIAMTCEGAAVHKRWVGVCGALASDPLATPVLIGLGVSELSVSPVQIGEIKD
RVRQLHEAECQRLARDLLKLSSAAAVRHACHQHWPLR
>Mature_837_residues
MHNNNKELTLSAPLSGPVLTLAKVPDAVFASGAMGDGIAIDPLNDTLYSPCAGVVIHVARTGHALTVRADNGAELLLHLG
LDTVELQGEGFSMLVKEGARVSNGQPLLRYDLDKVGRQCKSLVSLLILTNSQDFQARPITLKTVKVGEPLLHIVARHSAA
ANTEELGGPQVHGHVKVAHRGGLHARPAALIRQTAQGFKSQSQLHFAGKSAPCNSLIGLMGLAIGEQDEVQVSCQGPDAQ
AALQALLVALATALPEDHHAAAPLAATPRNRPAEAGVLHGVCAAPGLVGGPLFRLNAISLPADSGNHQPEQQLQILDTAL
NQVRSEIDGTLAQAKKQRNADEEAIFAAHLALLEDPALLDAARQSIETGTAATHAWSQSIDAQCQVLQNTGSPLLAERAN
DLRDLKQRVLRALLGEAWHYDVPAGAIVAAHELTPSDLLQLSAQGVAGLCMAEGGATSHVAILARGKGLPCMVALGSVLL
DQTQGQSVVLDADGGRLELTPNAERLAEVQQAQIDRQQRRDAQQAQAHLPAETRNGVAIEVVANVASSHEAADAFANGAD
GVGLLRTEFLFVDRHTAPDVEEQRAAYQAVIDAMGDKSVIIRTIDVGGDKQLDYLPLPVEANPVLGLRGIRLAQARPEIL
DQQLRALLQVSPLQRCRILLPMVTEVDELLHIRQRVDALCLELGINQRPEIGVMIEVPAAALQAEQLAEHADFLSIGTND
LSQYTLAMDRDHAGLAARVDALHPALLRLIAMTCEGAAVHKRWVGVCGALASDPLATPVLIGLGVSELSVSPVQIGEIKD
RVRQLHEAECQRLARDLLKLSSAAAVRHACHQHWPLR

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=566, Percent_Identity=35.3356890459364, Blast_Score=325, Evalue=8e-90,
Organism=Escherichia coli, GI48994992, Length=676, Percent_Identity=32.1005917159763, Blast_Score=308, Evalue=7e-85,
Organism=Escherichia coli, GI1788726, Length=663, Percent_Identity=31.8250377073906, Blast_Score=298, Evalue=7e-82,
Organism=Escherichia coli, GI1789193, Length=527, Percent_Identity=34.9146110056926, Blast_Score=265, Evalue=1e-71,
Organism=Escherichia coli, GI1786894, Length=126, Percent_Identity=43.6507936507937, Blast_Score=124, Evalue=3e-29,
Organism=Escherichia coli, GI1788757, Length=143, Percent_Identity=37.7622377622378, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI1790159, Length=146, Percent_Identity=36.3013698630137, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787994, Length=440, Percent_Identity=25, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI226510935, Length=372, Percent_Identity=26.8817204301075, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 88882; Mature: 88882

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00099 THIOLASE_3 ; PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER ; PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1 ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNNNKELTLSAPLSGPVLTLAKVPDAVFASGAMGDGIAIDPLNDTLYSPCAGVVIHVAR
CCCCCCEEEEECCCCCCEEEHHHCCHHHHHCCCCCCCEEECCCCHHHHCCHHHHEEEEEE
TGHALTVRADNGAELLLHLGLDTVELQGEGFSMLVKEGARVSNGQPLLRYDLDKVGRQCK
CCCEEEEEECCCCEEEEEECCCEEEECCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
SLVSLLILTNSQDFQARPITLKTVKVGEPLLHIVARHSAAANTEELGGPQVHGHVKVAHR
HHHHHHHEECCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCHHHCCCCEEECEEEEEEC
GGLHARPAALIRQTAQGFKSQSQLHFAGKSAPCNSLIGLMGLAIGEQDEVQVSCQGPDAQ
CCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHH
AALQALLVALATALPEDHHAAAPLAATPRNRPAEAGVLHGVCAAPGLVGGPLFRLNAISL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEC
PADSGNHQPEQQLQILDTALNQVRSEIDGTLAQAKKQRNADEEAIFAAHLALLEDPALLD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHH
AARQSIETGTAATHAWSQSIDAQCQVLQNTGSPLLAERANDLRDLKQRVLRALLGEAWHY
HHHHHHHCCCHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
DVPAGAIVAAHELTPSDLLQLSAQGVAGLCMAEGGATSHVAILARGKGLPCMVALGSVLL
CCCCCCEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHH
DQTQGQSVVLDADGGRLELTPNAERLAEVQQAQIDRQQRRDAQQAQAHLPAETRNGVAIE
HCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
VVANVASSHEAADAFANGADGVGLLRTEFLFVDRHTAPDVEEQRAAYQAVIDAMGDKSVI
EEHHHHHHHHHHHHHCCCCCCCCHHEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEE
IRTIDVGGDKQLDYLPLPVEANPVLGLRGIRLAQARPEILDQQLRALLQVSPLQRCRILL
EEEEECCCCCCCCEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCHHHHHHHHH
PMVTEVDELLHIRQRVDALCLELGINQRPEIGVMIEVPAAALQAEQLAEHADFLSIGTND
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHCEEECCCCC
LSQYTLAMDRDHAGLAARVDALHPALLRLIAMTCEGAAVHKRWVGVCGALASDPLATPVL
HHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHE
IGLGVSELSVSPVQIGEIKDRVRQLHEAECQRLARDLLKLSSAAAVRHACHQHWPLR
EECCHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHNNNKELTLSAPLSGPVLTLAKVPDAVFASGAMGDGIAIDPLNDTLYSPCAGVVIHVAR
CCCCCCEEEEECCCCCCEEEHHHCCHHHHHCCCCCCCEEECCCCHHHHCCHHHHEEEEEE
TGHALTVRADNGAELLLHLGLDTVELQGEGFSMLVKEGARVSNGQPLLRYDLDKVGRQCK
CCCEEEEEECCCCEEEEEECCCEEEECCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
SLVSLLILTNSQDFQARPITLKTVKVGEPLLHIVARHSAAANTEELGGPQVHGHVKVAHR
HHHHHHHEECCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCHHHCCCCEEECEEEEEEC
GGLHARPAALIRQTAQGFKSQSQLHFAGKSAPCNSLIGLMGLAIGEQDEVQVSCQGPDAQ
CCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHH
AALQALLVALATALPEDHHAAAPLAATPRNRPAEAGVLHGVCAAPGLVGGPLFRLNAISL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEC
PADSGNHQPEQQLQILDTALNQVRSEIDGTLAQAKKQRNADEEAIFAAHLALLEDPALLD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHH
AARQSIETGTAATHAWSQSIDAQCQVLQNTGSPLLAERANDLRDLKQRVLRALLGEAWHY
HHHHHHHCCCHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
DVPAGAIVAAHELTPSDLLQLSAQGVAGLCMAEGGATSHVAILARGKGLPCMVALGSVLL
CCCCCCEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHH
DQTQGQSVVLDADGGRLELTPNAERLAEVQQAQIDRQQRRDAQQAQAHLPAETRNGVAIE
HCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
VVANVASSHEAADAFANGADGVGLLRTEFLFVDRHTAPDVEEQRAAYQAVIDAMGDKSVI
EEHHHHHHHHHHHHHCCCCCCCCHHEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEE
IRTIDVGGDKQLDYLPLPVEANPVLGLRGIRLAQARPEILDQQLRALLQVSPLQRCRILL
EEEEECCCCCCCCEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCHHHHHHHHH
PMVTEVDELLHIRQRVDALCLELGINQRPEIGVMIEVPAAALQAEQLAEHADFLSIGTND
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHCEEECCCCC
LSQYTLAMDRDHAGLAARVDALHPALLRLIAMTCEGAAVHKRWVGVCGALASDPLATPVL
HHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHE
IGLGVSELSVSPVQIGEIKDRVRQLHEAECQRLARDLLKLSSAAAVRHACHQHWPLR
EECCHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2193161 [H]