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The map label for this gene is nagE [H]

Identifier: 77457230

GI number: 77457230

Start: 1166937

End: 1168655

Strand: Reverse

Name: nagE [H]

Synonym: Pfl01_1003

Alternate gene names: 77457230

Gene position: 1168655-1166937 (Counterclockwise)

Preceding gene: 77457231

Following gene: 77457225

Centisome position: 18.15

GC content: 62.94

Gene sequence:

>1719_bases
ATGTACCAACTCTTCATCGAAGGCCTGCAACGCCTCGGCCGTGCGCTGATGCTGCCGATCGCGATCTTGCCGATCGCCGG
CCTGCTGCTGCGCCTGGGCGACACCGACCTGCTGAACATCGCGATCATCCACGATGCCGGTCAGGTGATCTTCGCCAACC
TGGCAATGATCTTCGCCATCGGCATCGCCGTCGGTTTCGCCCGGGACAACAACGGCACCGCCGGCCTCGCCGGAGTGATC
GGCTACCTGGTGATGATTTCCACCCTCAAGGTGCTCGACGCCAGCATCAACATGGGCATGCTCGCCGGGATCGTCAGCGG
CCTGATGGCCGGCGCGCTGTACAACCGCTTCAAGGACATCAAGCTGCCGGAGTACCTGGCATTCTTCGGCGGCCGGCGTT
TCGTGCCCATCGTCACCGGGTTCAGCGCAGTCGGCCTGGGCGTGCTGTTCGGCTACATCTGGCCGCCAATCCAGCACGGC
ATCAACAGCTTCGGCACCCTGCTGATGGAAAGCGGCAGCTTCGGCGCGTTCGTGTTCGGCGTGTTCAACCGCCTGCTGAT
CGTCACCGGCCTGCACCACATCCTCAACAACATGGCGTGGTTCGTGTTCGGCAACTTCACCGACCCGACCACTGGCGCGC
TGGTGACCGGTGACCTGTCCCGCTACTTCGCCGGCGATCCGAAGGGCGGGCAGTTCATGACCGGCATGTTCCCGGTCATG
ATCTTCGGCCTCCCCGCCGCCTGCCTGGCGATGTACCGCAACGCCCTGCCGGAGCGGCGCAAAGTCATGGGCGGGATCTT
CCTGTCGATGGCCCTGACCGCATTCCTCACCGGCGTCACCGAACCGATCGAATTCGCCTTCATGTTCCTCGCACCGCTGC
TGTTTTTGCTGCACGCGCTGCTGACCGGGTTGTCGATGGCAATCACCAACGCACTGAACATCCACTTGGGCTTCACCTTC
TCCGGCGGCTTCATCGACATGGTGCTGGGCTGGGGACGCTCGACCAACGGCTGGCTGGTGGTGCCGGTAGGCCTTGCATA
TGCGGTCATCTACTACGCGGTGTTCGATTTCTGCATCCGCCGCTTCAACCTGAAAACCCCGGGACGTGAAGACGTCGCCA
CTGCCGAGAAATCCGTGCTGACAGAAAACGAGCGCGCCGCTGCGTACATCAAAGCCTTGGGAGGTGCCGAGAATCTGCTC
ACCGTCGGCGCCTGCACCACGCGTCTGCGACTGGAAATGGTCGATCGCAACAAAGCCTCCGATGCGGACTTGAAAGCACT
GGGCGCAATGGCCGTGGTACGTCCGGGCAAGGGCGGCAGTCTGCAGGTAGTCGTCGGGCCGCTGGCCGACAGCATTGCCG
ACGAAATCCGCCTGGCGATGCCCGCATTGGGTCGAGCCGCAGCGACTACTGCTCCTGTCGCCGTCGTCGAAACCAGCGAG
CCCGTTGCTATAGCCGCACCGCAGGCCCAGCAATGGCTGAACGCCCTGGGCGGCAGCGACAACGTGCTGCAACTCGACTG
CATCGCCACGAGCCGGATACGCCTGCAACTGGCCGATGGCAAAGCGCTGTCGGAAAGCCGGTTGAAAGAACTTGGATGTT
TGGGTGTGAGCGCGCTGGAGGATGGCGTGTGGCACTTGCTGGTAGGTGAGCGGGCGCAGAGTTTGAGTGTGGCGCTGGAA
GGGTTGGTTAATCGGAGTGAGGTGAGTGCGAAGGTCTGA

Upstream 100 bases:

>100_bases
TCGCCCGGGACCTGCTCAAGCTGAGCAGCGCCGCCGCGGTGCGTCATGCCTGTCATCAACATTGGCCTCTGCGCTAACAA
AAACAACAAGGACAAACGCC

Downstream 100 bases:

>100_bases
TGGTCGCCATCCAGTTTTGACGTGAAGCGGTTACAACCAGGGAACCCGGCCTAGGCCGGGTTTCCTGTTTATGCACATTA
ACCTCAAGACAGCTTTCCTC

Product: PTS system, N-acetylglucosamine-specific IIBC component

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MYQLFIEGLQRLGRALMLPIAILPIAGLLLRLGDTDLLNIAIIHDAGQVIFANLAMIFAIGIAVGFARDNNGTAGLAGVI
GYLVMISTLKVLDASINMGMLAGIVSGLMAGALYNRFKDIKLPEYLAFFGGRRFVPIVTGFSAVGLGVLFGYIWPPIQHG
INSFGTLLMESGSFGAFVFGVFNRLLIVTGLHHILNNMAWFVFGNFTDPTTGALVTGDLSRYFAGDPKGGQFMTGMFPVM
IFGLPAACLAMYRNALPERRKVMGGIFLSMALTAFLTGVTEPIEFAFMFLAPLLFLLHALLTGLSMAITNALNIHLGFTF
SGGFIDMVLGWGRSTNGWLVVPVGLAYAVIYYAVFDFCIRRFNLKTPGREDVATAEKSVLTENERAAAYIKALGGAENLL
TVGACTTRLRLEMVDRNKASDADLKALGAMAVVRPGKGGSLQVVVGPLADSIADEIRLAMPALGRAAATTAPVAVVETSE
PVAIAAPQAQQWLNALGGSDNVLQLDCIATSRIRLQLADGKALSESRLKELGCLGVSALEDGVWHLLVGERAQSLSVALE
GLVNRSEVSAKV

Sequences:

>Translated_572_residues
MYQLFIEGLQRLGRALMLPIAILPIAGLLLRLGDTDLLNIAIIHDAGQVIFANLAMIFAIGIAVGFARDNNGTAGLAGVI
GYLVMISTLKVLDASINMGMLAGIVSGLMAGALYNRFKDIKLPEYLAFFGGRRFVPIVTGFSAVGLGVLFGYIWPPIQHG
INSFGTLLMESGSFGAFVFGVFNRLLIVTGLHHILNNMAWFVFGNFTDPTTGALVTGDLSRYFAGDPKGGQFMTGMFPVM
IFGLPAACLAMYRNALPERRKVMGGIFLSMALTAFLTGVTEPIEFAFMFLAPLLFLLHALLTGLSMAITNALNIHLGFTF
SGGFIDMVLGWGRSTNGWLVVPVGLAYAVIYYAVFDFCIRRFNLKTPGREDVATAEKSVLTENERAAAYIKALGGAENLL
TVGACTTRLRLEMVDRNKASDADLKALGAMAVVRPGKGGSLQVVVGPLADSIADEIRLAMPALGRAAATTAPVAVVETSE
PVAIAAPQAQQWLNALGGSDNVLQLDCIATSRIRLQLADGKALSESRLKELGCLGVSALEDGVWHLLVGERAQSLSVALE
GLVNRSEVSAKV
>Mature_572_residues
MYQLFIEGLQRLGRALMLPIAILPIAGLLLRLGDTDLLNIAIIHDAGQVIFANLAMIFAIGIAVGFARDNNGTAGLAGVI
GYLVMISTLKVLDASINMGMLAGIVSGLMAGALYNRFKDIKLPEYLAFFGGRRFVPIVTGFSAVGLGVLFGYIWPPIQHG
INSFGTLLMESGSFGAFVFGVFNRLLIVTGLHHILNNMAWFVFGNFTDPTTGALVTGDLSRYFAGDPKGGQFMTGMFPVM
IFGLPAACLAMYRNALPERRKVMGGIFLSMALTAFLTGVTEPIEFAFMFLAPLLFLLHALLTGLSMAITNALNIHLGFTF
SGGFIDMVLGWGRSTNGWLVVPVGLAYAVIYYAVFDFCIRRFNLKTPGREDVATAEKSVLTENERAAAYIKALGGAENLL
TVGACTTRLRLEMVDRNKASDADLKALGAMAVVRPGKGGSLQVVVGPLADSIADEIRLAMPALGRAAATTAPVAVVETSE
PVAIAAPQAQQWLNALGGSDNVLQLDCIATSRIRLQLADGKALSESRLKELGCLGVSALEDGVWHLLVGERAQSLSVALE
GLVNRSEVSAKV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=459, Percent_Identity=52.2875816993464, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1787343, Length=476, Percent_Identity=41.8067226890756, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1787908, Length=512, Percent_Identity=35.546875, Blast_Score=267, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 60858; Mature: 60858

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQLFIEGLQRLGRALMLPIAILPIAGLLLRLGDTDLLNIAIIHDAGQVIFANLAMIFAI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHH
GIAVGFARDNNGTAGLAGVIGYLVMISTLKVLDASINMGMLAGIVSGLMAGALYNRFKDI
HHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
KLPEYLAFFGGRRFVPIVTGFSAVGLGVLFGYIWPPIQHGINSFGTLLMESGSFGAFVFG
CCHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHH
VFNRLLIVTGLHHILNNMAWFVFGNFTDPTTGALVTGDLSRYFAGDPKGGQFMTGMFPVM
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEECCHHHHHCCCCCCCCCHHHHHHHH
IFGLPAACLAMYRNALPERRKVMGGIFLSMALTAFLTGVTEPIEFAFMFLAPLLFLLHAL
HHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LTGLSMAITNALNIHLGFTFSGGFIDMVLGWGRSTNGWLVVPVGLAYAVIYYAVFDFCIR
HHHHHHHHHHHEEEEEEEEECCCHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
RFNLKTPGREDVATAEKSVLTENERAAAYIKALGGAENLLTVGACTTRLRLEMVDRNKAS
HCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEHHHHHHHHEEEECCCCCCC
DADLKALGAMAVVRPGKGGSLQVVVGPLADSIADEIRLAMPALGRAAATTAPVAVVETSE
CCHHHHHCCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCC
PVAIAAPQAQQWLNALGGSDNVLQLDCIATSRIRLQLADGKALSESRLKELGCLGVSALE
CEEEECCHHHHHHHHHCCCCCEEEEEEEEECEEEEEECCCCCCHHHHHHHHHCCCHHHHH
DGVWHLLVGERAQSLSVALEGLVNRSEVSAKV
CCCEEEEECCCHHHHHHHHHHHHCHHHHCCCC
>Mature Secondary Structure
MYQLFIEGLQRLGRALMLPIAILPIAGLLLRLGDTDLLNIAIIHDAGQVIFANLAMIFAI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHH
GIAVGFARDNNGTAGLAGVIGYLVMISTLKVLDASINMGMLAGIVSGLMAGALYNRFKDI
HHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
KLPEYLAFFGGRRFVPIVTGFSAVGLGVLFGYIWPPIQHGINSFGTLLMESGSFGAFVFG
CCHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHH
VFNRLLIVTGLHHILNNMAWFVFGNFTDPTTGALVTGDLSRYFAGDPKGGQFMTGMFPVM
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEECCHHHHHCCCCCCCCCHHHHHHHH
IFGLPAACLAMYRNALPERRKVMGGIFLSMALTAFLTGVTEPIEFAFMFLAPLLFLLHAL
HHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LTGLSMAITNALNIHLGFTFSGGFIDMVLGWGRSTNGWLVVPVGLAYAVIYYAVFDFCIR
HHHHHHHHHHHEEEEEEEEECCCHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
RFNLKTPGREDVATAEKSVLTENERAAAYIKALGGAENLLTVGACTTRLRLEMVDRNKAS
HCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEHHHHHHHHEEEECCCCCCC
DADLKALGAMAVVRPGKGGSLQVVVGPLADSIADEIRLAMPALGRAAATTAPVAVVETSE
CCHHHHHCCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCC
PVAIAAPQAQQWLNALGGSDNVLQLDCIATSRIRLQLADGKALSESRLKELGCLGVSALE
CEEEECCHHHHHHHHHCCCCCEEEEEEEEECEEEEEECCCCCCHHHHHHHHHCCCHHHHH
DGVWHLLVGERAQSLSVALEGLVNRSEVSAKV
CCCEEEEECCCHHHHHHHHHHHHCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1745234 [H]