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The map label for this gene is mltF

Identifier: 77457225

GI number: 77457225

Start: 1159565

End: 1161025

Strand: Reverse

Name: mltF

Synonym: Pfl01_0998

Alternate gene names: 77457225

Gene position: 1161025-1159565 (Counterclockwise)

Preceding gene: 77457230

Following gene: 77457216

Centisome position: 18.03

GC content: 60.44

Gene sequence:

>1461_bases
ATGTTTTCCCCAACGGCTTTGCGTCCGCGATACGCCAAATGGCTGATCGCTACCGGACTCTTCCTGATGCTCAGTGGCTG
TGTTGATAAACCCAACACACTCGAGCGCGTAAAGGAGGATGGCGTGCTGCGGGTGGTCACCCGAAACAGCCCCGCCACCT
ACTTTCAGGATCGCAGCGGTGAAACCGGCTTCGAATACGAGCTGGTGAAGCGCTTCGCCGACGATTTGGGGGTCGAGCTC
AAGATTGAAACCGCCGACAACCTCGACGACCTGTTCAACCAGGTCGGCAAGCCGAACGGCCCGGTACTGGCGGCTGCCGG
TCTGGTCAGCAGCGAACAGCGCAAGAAGCAGGTGCGTTTCTCCCGCTCCTACCTCGAAGTCACCCCGCAGATCATCTATC
GCAACGGTCAGTCACGCCCTACCGATGCGGGCGATCTGGTGGGCAAGAAGATCATGGTGCTCAAGGGCAGCACCCACGCC
GAACAACTGGCGGAGCTGAAACAGAAATACCCTGGCATCCAGTACGAAGAGTCCGACGCCGTTGAAGTGGTCGACCTGCT
GCGCATGGTCGATGAAGGCCAGATCGACCTGACCCTGGTCGATTCCAACGAAGTGGCGATGAACCAGGTGTACTTCACCA
ACATCCGCGTGGCCTTTGACCTCGGCGACGCGCGCAGCCAGAGCTGGGCCGTGGCCGCCGGCGAAGACAACAGCCTGCTC
AACGAGATCAACAGTTATCTGGACAAGGTGCAGAAGAACGGCACCCTGCAACGCCTGAAAGACCGTTATTACGGCCATGT
CGACGTCCTCGGCTACATGGGCGCCACCACCTTCGCCCAGCATTTGCAGCAGCGGTTGCCCAAGTACGAACAGCATTTCA
AGGCCTACGCCAAGAAAGAGAAAGTCGACTGGCGCCTGCTGGCGGCGATCGGTTATCAGGAATCGCTGTGGCAACCGGCG
GTCACGTCGAAGACCGGCGTGCGCGGGCTGATGATGCTGACCCAGAACACCGCCCAGGCCATGGGCGTGTCCAACCGCCT
CGATCCGAAGCAAAGCATCATGGGCGGTGCCAAGTACCTGGCCTACATGAAGGATCAGCTCGACGAGTCGATCCAGGAGC
CGGATCGCACATGGTTTGCCCTGGCGGCCTACAACGTCGGCAGCGGTCATCTGGATGACGCACGCAAACTGGCTGCCAAG
GAAGGGTTGAACCCGGACAAGTGGCTGGATGTGAAAAAGATCCTGCCGCGCCTGTCGCAGAAGCAGTGGTACAGCAAGAC
CCGCTACGGCTACGCCCGGGGCGGCGAACCGGTGCATTTCGTGGCGAACATCCGTCGCTACTACGACATCCTCACCTGGG
TGACCCAGCCGCAGCTTGAAGGCGATCAGGTGGCCGAGGGCAACCTGCATGTGCCGGGGATCGACAAGTCGAAACCCGCT
CAAGAGCCCGCCCCGCTTTAA

Upstream 100 bases:

>100_bases
GCCCGTTTCTGCGGGGTTTCGCGCGGTTTTGTGATGCCTAGCAGACAACCCCGCCACTGTCGAGATATGGCGCCCGTTGT
CCTTTGCGTATACTGCGCAC

Downstream 100 bases:

>100_bases
CCCATTCCAAAACACATGTGGGAGCAAACTCGCTCCCACAAGATGTTGAGACTTAGTGCCTGGCAGCCGCCAGAATCAGC
GTCTTCATCTCCGACACCGC

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MFSPTALRPRYAKWLIATGLFLMLSGCVDKPNTLERVKEDGVLRVVTRNSPATYFQDRSGETGFEYELVKRFADDLGVEL
KIETADNLDDLFNQVGKPNGPVLAAAGLVSSEQRKKQVRFSRSYLEVTPQIIYRNGQSRPTDAGDLVGKKIMVLKGSTHA
EQLAELKQKYPGIQYEESDAVEVVDLLRMVDEGQIDLTLVDSNEVAMNQVYFTNIRVAFDLGDARSQSWAVAAGEDNSLL
NEINSYLDKVQKNGTLQRLKDRYYGHVDVLGYMGATTFAQHLQQRLPKYEQHFKAYAKKEKVDWRLLAAIGYQESLWQPA
VTSKTGVRGLMMLTQNTAQAMGVSNRLDPKQSIMGGAKYLAYMKDQLDESIQEPDRTWFALAAYNVGSGHLDDARKLAAK
EGLNPDKWLDVKKILPRLSQKQWYSKTRYGYARGGEPVHFVANIRRYYDILTWVTQPQLEGDQVAEGNLHVPGIDKSKPA
QEPAPL

Sequences:

>Translated_486_residues
MFSPTALRPRYAKWLIATGLFLMLSGCVDKPNTLERVKEDGVLRVVTRNSPATYFQDRSGETGFEYELVKRFADDLGVEL
KIETADNLDDLFNQVGKPNGPVLAAAGLVSSEQRKKQVRFSRSYLEVTPQIIYRNGQSRPTDAGDLVGKKIMVLKGSTHA
EQLAELKQKYPGIQYEESDAVEVVDLLRMVDEGQIDLTLVDSNEVAMNQVYFTNIRVAFDLGDARSQSWAVAAGEDNSLL
NEINSYLDKVQKNGTLQRLKDRYYGHVDVLGYMGATTFAQHLQQRLPKYEQHFKAYAKKEKVDWRLLAAIGYQESLWQPA
VTSKTGVRGLMMLTQNTAQAMGVSNRLDPKQSIMGGAKYLAYMKDQLDESIQEPDRTWFALAAYNVGSGHLDDARKLAAK
EGLNPDKWLDVKKILPRLSQKQWYSKTRYGYARGGEPVHFVANIRRYYDILTWVTQPQLEGDQVAEGNLHVPGIDKSKPA
QEPAPL
>Mature_486_residues
MFSPTALRPRYAKWLIATGLFLMLSGCVDKPNTLERVKEDGVLRVVTRNSPATYFQDRSGETGFEYELVKRFADDLGVEL
KIETADNLDDLFNQVGKPNGPVLAAAGLVSSEQRKKQVRFSRSYLEVTPQIIYRNGQSRPTDAGDLVGKKIMVLKGSTHA
EQLAELKQKYPGIQYEESDAVEVVDLLRMVDEGQIDLTLVDSNEVAMNQVYFTNIRVAFDLGDARSQSWAVAAGEDNSLL
NEINSYLDKVQKNGTLQRLKDRYYGHVDVLGYMGATTFAQHLQQRLPKYEQHFKAYAKKEKVDWRLLAAIGYQESLWQPA
VTSKTGVRGLMMLTQNTAQAMGVSNRLDPKQSIMGGAKYLAYMKDQLDESIQEPDRTWFALAAYNVGSGHLDDARKLAAK
EGLNPDKWLDVKKILPRLSQKQWYSKTRYGYARGGEPVHFVANIRRYYDILTWVTQPQLEGDQVAEGNLHVPGIDKSKPA
QEPAPL

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family

Homologues:

Organism=Escherichia coli, GI171474010, Length=417, Percent_Identity=41.4868105515587, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1788228, Length=250, Percent_Identity=31.6, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MLTF_PSEPF (Q3KHL5)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_346730.1
- ProteinModelPortal:   Q3KHL5
- SMR:   Q3KHL5
- STRING:   Q3KHL5
- GeneID:   3716000
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_0998
- eggNOG:   COG4623
- HOGENOM:   HBG644469
- OMA:   QYVENIR
- ProtClustDB:   PRK10859
- BioCyc:   PFLU205922:PFL_0998-MONOMER
- HAMAP:   MF_02016
- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189
- SMART:   SM00062

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT

EC number: NA

Molecular weight: Translated: 54725; Mature: 54725

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: ACT_SITE 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSPTALRPRYAKWLIATGLFLMLSGCVDKPNTLERVKEDGVLRVVTRNSPATYFQDRSG
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCCCCCEEECCCC
ETGFEYELVKRFADDLGVELKIETADNLDDLFNQVGKPNGPVLAAAGLVSSEQRKKQVRF
CCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHH
SRSYLEVTPQIIYRNGQSRPTDAGDLVGKKIMVLKGSTHAEQLAELKQKYPGIQYEESDA
HHHHHHHCHHHEEECCCCCCCCHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCH
VEVVDLLRMVDEGQIDLTLVDSNEVAMNQVYFTNIRVAFDLGDARSQSWAVAAGEDNSLL
HHHHHHHHHHCCCCEEEEEECCCCEEEEEEEEEEEEEEEECCCCCCCCEEEECCCCHHHH
NEINSYLDKVQKNGTLQRLKDRYYGHVDVLGYMGATTFAQHLQQRLPKYEQHFKAYAKKE
HHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
KVDWRLLAAIGYQESLWQPAVTSKTGVRGLMMLTQNTAQAMGVSNRLDPKQSIMGGAKYL
CCCEEEEEHHCCHHHHCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHH
AYMKDQLDESIQEPDRTWFALAAYNVGSGHLDDARKLAAKEGLNPDKWLDVKKILPRLSQ
HHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
KQWYSKTRYGYARGGEPVHFVANIRRYYDILTWVTQPQLEGDQVAEGNLHVPGIDKSKPA
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCC
QEPAPL
CCCCCC
>Mature Secondary Structure
MFSPTALRPRYAKWLIATGLFLMLSGCVDKPNTLERVKEDGVLRVVTRNSPATYFQDRSG
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCCCCCEEECCCC
ETGFEYELVKRFADDLGVELKIETADNLDDLFNQVGKPNGPVLAAAGLVSSEQRKKQVRF
CCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHH
SRSYLEVTPQIIYRNGQSRPTDAGDLVGKKIMVLKGSTHAEQLAELKQKYPGIQYEESDA
HHHHHHHCHHHEEECCCCCCCCHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCH
VEVVDLLRMVDEGQIDLTLVDSNEVAMNQVYFTNIRVAFDLGDARSQSWAVAAGEDNSLL
HHHHHHHHHHCCCCEEEEEECCCCEEEEEEEEEEEEEEEECCCCCCCCEEEECCCCHHHH
NEINSYLDKVQKNGTLQRLKDRYYGHVDVLGYMGATTFAQHLQQRLPKYEQHFKAYAKKE
HHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
KVDWRLLAAIGYQESLWQPAVTSKTGVRGLMMLTQNTAQAMGVSNRLDPKQSIMGGAKYL
CCCEEEEEHHCCHHHHCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHH
AYMKDQLDESIQEPDRTWFALAAYNVGSGHLDDARKLAAKEGLNPDKWLDVKKILPRLSQ
HHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
KQWYSKTRYGYARGGEPVHFVANIRRYYDILTWVTQPQLEGDQVAEGNLHVPGIDKSKPA
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCC
QEPAPL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA