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The map label for this gene is prfC [H]

Identifier: 77457034

GI number: 77457034

Start: 951785

End: 953368

Strand: Direct

Name: prfC [H]

Synonym: Pfl01_0807

Alternate gene names: 77457034

Gene position: 951785-953368 (Clockwise)

Preceding gene: 77457029

Following gene: 77457035

Centisome position: 14.78

GC content: 60.61

Gene sequence:

>1584_bases
ATGACCAGACAGGCCGCCGAAGTCGCGAAACGCCGCACTTTCGCCATTATTTCCCACCCCGATGCCGGTAAAACCACCAT
CACCGAAAAGCTCTTGCTGATGGGCAAGGCGATTGCCGTGGCCGGTACGGTGAAATCCCGTAAATCCGACCGCCACGCGA
CATCCGACTGGATGGAGATGGAAAAGCAGCGGGGCATCTCGATTACTACGTCGGTCATGCAGTTCCCGTATCGCGAACAC
ATGATCAACCTGCTCGACACCCCGGGCCACGAAGACTTCTCCGAAGACACCTACCGCACTCTGACTGCGGTGGACTCGGC
ATTGATGGTCCTCGACGGCGGTAAAGGTGTAGAGCCACGGACCATCGCCCTGATGGACGTCTGCCGTCTGCGTGACACGC
CGATCGTCAGCTTCATCAACAAACTCGACCGCGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTCCTG
AAGATCAAGGCTGCGCCGATCACCTGGCCGATCGGTTGCTACCGCGACTTCAAGGGCGTGTACCACCTGGCCGACGACTA
CATCATTGTCTACACCGCCGGTCACGGTCATGAACGCACCGAAACCAAGATCATTGAGAAGCTCGATTCCGACGAAGCCC
GCGCGCATCTGGGCGACGAGTACGAGCGTTTCCTCGAACAGCTGGAACTGGTGCAGGGCGCCTGCCACGAGTTCAATCAG
CAGGAATTCCTCGACGGCCAACTGACCCCGGTGTTCTTCGGTACTGCATTGGGCAACTTCGGTGTCGATCACGTACTCGA
CGCCGTGGTCGACTGGGCCCCGCGTCCGCTGCCACGCGTGGCCAACGAGCGCACCGTGGAGCCGGTGGAAGAGAAGTTCT
CGGGCTTCATCTTCAAGATCCAGGCGAACATGGACCCGAAACACCGCGACCGCATCGCCTTCATGCGTATCTGCTCCGGC
AAGTACGAAAAAGGCATGAAGATGCGCCACGTGCGTACCGGCAAGGACGTGCGCATCGGCGACGCCCTGACATTCTTCTC
CTCCGAGCGTGAACAGCTGGAAGAAGCGTACGCCGGCGACATCATCGGTCTGCACAACCACGGCACGATCCAGATCGGCG
ACACCTTCAGCGAAGGCGAAACCCTGGGCTTCACCGGTATCCCGCACTTCGCCCCGGAACTGTTCCGTCGCGTACGCCTG
CGCGACCCGCTGAAATCCAAGCAACTGCGCCAGGGCCTGCAACAGCTCGCCGAAGAAGGCGCGACCCAGGTGTTCTTCCC
GGAGCGCAGCAACGACATCATCCTCGGCGCCGTCGGTGTGCTGCAGTTCGATGTGGTCGCCAGCCGCCTGAAAGAGGAAT
ACAAGGTCGAGTGCTCCTACGAGCCGATCACCGTTTACTCCGCGCGCTGGATCGAATGCAGCGACAAGAAGAAGCTCGAG
GAATTCTCCAACAAGGCGGTGGAAAACCTGGCACTGGACGGTGGCGGTCACCTGACCTACCTCGCGCCGACCCGGGTCAA
CCTGGCCCTGATGGAAGAGCGCTGGCCGGACGTGAAATTCCGTGCGACGCGCGAGCATCACTAA

Upstream 100 bases:

>100_bases
GCAGCATTTGGCCACCCTTAATGTGCTCAACGGTTCGGTTATTGAATCGAGTTGGGCTATAATCGCCGCCCTTTTTTGAA
TCACCGCCAGGCGATTTCCC

Downstream 100 bases:

>100_bases
GCGCTGAGTTGCTCTATCCAAAGCCCCCGATGCGCAAGCCTCGGGGGCTTTTTGTTGGCTGCAGAAACGGTTGCTCACCA
GGCGTCCGAGAGTGGCGACC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MTRQAAEVAKRRTFAIISHPDAGKTTITEKLLLMGKAIAVAGTVKSRKSDRHATSDWMEMEKQRGISITTSVMQFPYREH
MINLLDTPGHEDFSEDTYRTLTAVDSALMVLDGGKGVEPRTIALMDVCRLRDTPIVSFINKLDRDIRDPIELLDEIEAVL
KIKAAPITWPIGCYRDFKGVYHLADDYIIVYTAGHGHERTETKIIEKLDSDEARAHLGDEYERFLEQLELVQGACHEFNQ
QEFLDGQLTPVFFGTALGNFGVDHVLDAVVDWAPRPLPRVANERTVEPVEEKFSGFIFKIQANMDPKHRDRIAFMRICSG
KYEKGMKMRHVRTGKDVRIGDALTFFSSEREQLEEAYAGDIIGLHNHGTIQIGDTFSEGETLGFTGIPHFAPELFRRVRL
RDPLKSKQLRQGLQQLAEEGATQVFFPERSNDIILGAVGVLQFDVVASRLKEEYKVECSYEPITVYSARWIECSDKKKLE
EFSNKAVENLALDGGGHLTYLAPTRVNLALMEERWPDVKFRATREHH

Sequences:

>Translated_527_residues
MTRQAAEVAKRRTFAIISHPDAGKTTITEKLLLMGKAIAVAGTVKSRKSDRHATSDWMEMEKQRGISITTSVMQFPYREH
MINLLDTPGHEDFSEDTYRTLTAVDSALMVLDGGKGVEPRTIALMDVCRLRDTPIVSFINKLDRDIRDPIELLDEIEAVL
KIKAAPITWPIGCYRDFKGVYHLADDYIIVYTAGHGHERTETKIIEKLDSDEARAHLGDEYERFLEQLELVQGACHEFNQ
QEFLDGQLTPVFFGTALGNFGVDHVLDAVVDWAPRPLPRVANERTVEPVEEKFSGFIFKIQANMDPKHRDRIAFMRICSG
KYEKGMKMRHVRTGKDVRIGDALTFFSSEREQLEEAYAGDIIGLHNHGTIQIGDTFSEGETLGFTGIPHFAPELFRRVRL
RDPLKSKQLRQGLQQLAEEGATQVFFPERSNDIILGAVGVLQFDVVASRLKEEYKVECSYEPITVYSARWIECSDKKKLE
EFSNKAVENLALDGGGHLTYLAPTRVNLALMEERWPDVKFRATREHH
>Mature_526_residues
TRQAAEVAKRRTFAIISHPDAGKTTITEKLLLMGKAIAVAGTVKSRKSDRHATSDWMEMEKQRGISITTSVMQFPYREHM
INLLDTPGHEDFSEDTYRTLTAVDSALMVLDGGKGVEPRTIALMDVCRLRDTPIVSFINKLDRDIRDPIELLDEIEAVLK
IKAAPITWPIGCYRDFKGVYHLADDYIIVYTAGHGHERTETKIIEKLDSDEARAHLGDEYERFLEQLELVQGACHEFNQQ
EFLDGQLTPVFFGTALGNFGVDHVLDAVVDWAPRPLPRVANERTVEPVEEKFSGFIFKIQANMDPKHRDRIAFMRICSGK
YEKGMKMRHVRTGKDVRIGDALTFFSSEREQLEEAYAGDIIGLHNHGTIQIGDTFSEGETLGFTGIPHFAPELFRRVRLR
DPLKSKQLRQGLQQLAEEGATQVFFPERSNDIILGAVGVLQFDVVASRLKEEYKVECSYEPITVYSARWIECSDKKKLEE
FSNKAVENLALDGGGHLTYLAPTRVNLALMEERWPDVKFRATREHH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=485, Percent_Identity=28.4536082474227, Blast_Score=172, Evalue=7e-43,
Organism=Homo sapiens, GI19923640, Length=514, Percent_Identity=27.0428015564202, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI25306283, Length=399, Percent_Identity=29.0726817042606, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI25306287, Length=486, Percent_Identity=25.9259259259259, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI157426893, Length=155, Percent_Identity=30.9677419354839, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI94966754, Length=150, Percent_Identity=32.6666666666667, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1790835, Length=521, Percent_Identity=67.7543186180422, Blast_Score=748, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=493, Percent_Identity=29.0060851926978, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1788922, Length=158, Percent_Identity=34.1772151898734, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=34.2281879194631, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=496, Percent_Identity=28.0241935483871, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17556745, Length=178, Percent_Identity=33.7078651685393, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17557151, Length=134, Percent_Identity=32.089552238806, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=30.0751879699248, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=30.0751879699248, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=476, Percent_Identity=29.4117647058824, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6322359, Length=526, Percent_Identity=25.2851711026616, Blast_Score=128, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=27.1523178807947, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324707, Length=156, Percent_Identity=31.4102564102564, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320593, Length=156, Percent_Identity=31.4102564102564, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24582462, Length=476, Percent_Identity=29.8319327731092, Blast_Score=185, Evalue=8e-47,
Organism=Drosophila melanogaster, GI221458488, Length=515, Percent_Identity=24.8543689320388, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=30.8176100628931, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28574573, Length=169, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59828; Mature: 59697

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRQAAEVAKRRTFAIISHPDAGKTTITEKLLLMGKAIAVAGTVKSRKSDRHATSDWMEM
CCCHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
EKQRGISITTSVMQFPYREHMINLLDTPGHEDFSEDTYRTLTAVDSALMVLDGGKGVEPR
HHHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCH
TIALMDVCRLRDTPIVSFINKLDRDIRDPIELLDEIEAVLKIKAAPITWPIGCYRDFKGV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHH
YHLADDYIIVYTAGHGHERTETKIIEKLDSDEARAHLGDEYERFLEQLELVQGACHEFNQ
HEECCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCH
QEFLDGQLTPVFFGTALGNFGVDHVLDAVVDWAPRPLPRVANERTVEPVEEKFSGFIFKI
HHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHCCEEEEE
QANMDPKHRDRIAFMRICSGKYEKGMKMRHVRTGKDVRIGDALTFFSSEREQLEEAYAGD
ECCCCCCHHHHHHHHHHHCCHHHCCCEEEEECCCCCEEECHHHHHHHHHHHHHHHHHCCC
IIGLHNHGTIQIGDTFSEGETLGFTGIPHFAPELFRRVRLRDPLKSKQLRQGLQQLAEEG
EEEECCCCEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
ATQVFFPERSNDIILGAVGVLQFDVVASRLKEEYKVECSYEPITVYSARWIECSDKKKLE
CCEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEEEECCCHHHHH
EFSNKAVENLALDGGGHLTYLAPTRVNLALMEERWPDVKFRATREHH
HHHHHHHHHHEECCCCEEEEECCCCEEEEEHHHCCCCCEEECCCCCC
>Mature Secondary Structure 
TRQAAEVAKRRTFAIISHPDAGKTTITEKLLLMGKAIAVAGTVKSRKSDRHATSDWMEM
CCHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
EKQRGISITTSVMQFPYREHMINLLDTPGHEDFSEDTYRTLTAVDSALMVLDGGKGVEPR
HHHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCH
TIALMDVCRLRDTPIVSFINKLDRDIRDPIELLDEIEAVLKIKAAPITWPIGCYRDFKGV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHH
YHLADDYIIVYTAGHGHERTETKIIEKLDSDEARAHLGDEYERFLEQLELVQGACHEFNQ
HEECCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCH
QEFLDGQLTPVFFGTALGNFGVDHVLDAVVDWAPRPLPRVANERTVEPVEEKFSGFIFKI
HHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHCCEEEEE
QANMDPKHRDRIAFMRICSGKYEKGMKMRHVRTGKDVRIGDALTFFSSEREQLEEAYAGD
ECCCCCCHHHHHHHHHHHCCHHHCCCEEEEECCCCCEEECHHHHHHHHHHHHHHHHHCCC
IIGLHNHGTIQIGDTFSEGETLGFTGIPHFAPELFRRVRLRDPLKSKQLRQGLQQLAEEG
EEEECCCCEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
ATQVFFPERSNDIILGAVGVLQFDVVASRLKEEYKVECSYEPITVYSARWIECSDKKKLE
CCEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEEEECCCHHHHH
EFSNKAVENLALDGGGHLTYLAPTRVNLALMEERWPDVKFRATREHH
HHHHHHHHHHEECCCCEEEEECCCCEEEEEHHHCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA