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The map label for this gene is gcvA [H]

Identifier: 77456777

GI number: 77456777

Start: 641371

End: 642276

Strand: Reverse

Name: gcvA [H]

Synonym: Pfl01_0549

Alternate gene names: 77456777

Gene position: 642276-641371 (Counterclockwise)

Preceding gene: 77456787

Following gene: 77456776

Centisome position: 9.98

GC content: 61.59

Gene sequence:

>906_bases
ATGCTGAACAAACGCCACTTGCCGTCGATCACCGCGTTGCAGTGCTTCGAGGCCGTGACCCGGCACCTGAGTTTCACCCG
GGCTGCCGAGGAACTGAACCTGACCCAGAGCGCTGTCAGCAAGCAGGTGGCGCAGCTCGAAGAATTGCTGCAGCACTTGT
TGTTCCGTCGGGTTCGCCGTCGTTTGCAGATGACCCCGGCCGGCGATCTCTACTTGGTGGAAGTCAGAAAAATCCTGACC
CAGGTCGAGATGTCGACCCATTACCTGCGTTCCTACGGCGGCGAAACCGAAGTCCTGCGCGTCTCCACGCCCTCGACCTT
CGGCGCCCGCTGGCTGGTGCCGCGCCTCAAGGGCTGGCGTCTGCGTCATCCATCGATTCATCTGGACCTGTGCAACGAGC
AGGAGGCCGATGACTTGCTGCAAGGGCGCAGCGACCTGGCGTTCTATTTCGGCCAGGGTTCTCGCCCCGGCACCGAATGC
CTGAAGCTGTTCGGCGAAGAATTGGTGCCAGTCTGCGCGCCGGGCAGCCTGCCGGACACTCCGTTGACTGATCCAACGCA
ACTCACCGATCTGGTCCTGCTGCAGAATGCTTCGCGCCCGCAAGCGTGGCACGACTGGTTCGACAGCCAGGGTTACCAGA
CCGAACACAGCTACCACGGCCCGCGCTTCGAAACCTTTTATATGTGCATCCGCGCCGCCCAGGTCGGCTGTGGCGTCGCC
CTGCTGCCGAGGTTTCTGGTGGAAGAGGAATTGGCCGACGGCAAACTGGTCATTCCCTGGCAGCATGCAATGCCCAGCAC
CGATGCGTATTACCTGGCTTATCCGGAACACGCGGCGGAAGTGCCCAAGGTGCGGGATTTCGTGAAGTGGATGCTGGAGC
AGATCGACAGTCCTGATGCGGTCTGA

Upstream 100 bases:

>100_bases
TTTAGTTGTAGCGTCAGGAAGGCTGTGCAACAAACGACCTTTACGCGAGATATCATTCCGTTTATTCATGCTGCGCAGAA
AGACAAGAAAAGGATCGCCC

Downstream 100 bases:

>100_bases
GGCGCTGTGCCGGGATAAAAATCGCTGGCAATCCCCACCCCGGATATGCGCCACTAGCGCCATTGATTGATACCGCGCCA
GAGCGCGGCCCGCCTGGAGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MLNKRHLPSITALQCFEAVTRHLSFTRAAEELNLTQSAVSKQVAQLEELLQHLLFRRVRRRLQMTPAGDLYLVEVRKILT
QVEMSTHYLRSYGGETEVLRVSTPSTFGARWLVPRLKGWRLRHPSIHLDLCNEQEADDLLQGRSDLAFYFGQGSRPGTEC
LKLFGEELVPVCAPGSLPDTPLTDPTQLTDLVLLQNASRPQAWHDWFDSQGYQTEHSYHGPRFETFYMCIRAAQVGCGVA
LLPRFLVEEELADGKLVIPWQHAMPSTDAYYLAYPEHAAEVPKVRDFVKWMLEQIDSPDAV

Sequences:

>Translated_301_residues
MLNKRHLPSITALQCFEAVTRHLSFTRAAEELNLTQSAVSKQVAQLEELLQHLLFRRVRRRLQMTPAGDLYLVEVRKILT
QVEMSTHYLRSYGGETEVLRVSTPSTFGARWLVPRLKGWRLRHPSIHLDLCNEQEADDLLQGRSDLAFYFGQGSRPGTEC
LKLFGEELVPVCAPGSLPDTPLTDPTQLTDLVLLQNASRPQAWHDWFDSQGYQTEHSYHGPRFETFYMCIRAAQVGCGVA
LLPRFLVEEELADGKLVIPWQHAMPSTDAYYLAYPEHAAEVPKVRDFVKWMLEQIDSPDAV
>Mature_301_residues
MLNKRHLPSITALQCFEAVTRHLSFTRAAEELNLTQSAVSKQVAQLEELLQHLLFRRVRRRLQMTPAGDLYLVEVRKILT
QVEMSTHYLRSYGGETEVLRVSTPSTFGARWLVPRLKGWRLRHPSIHLDLCNEQEADDLLQGRSDLAFYFGQGSRPGTEC
LKLFGEELVPVCAPGSLPDTPLTDPTQLTDLVLLQNASRPQAWHDWFDSQGYQTEHSYHGPRFETFYMCIRAAQVGCGVA
LLPRFLVEEELADGKLVIPWQHAMPSTDAYYLAYPEHAAEVPKVRDFVKWMLEQIDSPDAV

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=36.426116838488, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=31.4878892733564, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=29.7297297297297, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=23.8095238095238, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693193, Length=297, Percent_Identity=26.2626262626263, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788887, Length=303, Percent_Identity=26.0726072607261, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787806, Length=255, Percent_Identity=27.4509803921569, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI157672245, Length=165, Percent_Identity=30.9090909090909, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082132, Length=290, Percent_Identity=25.1724137931034, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787589, Length=277, Percent_Identity=25.2707581227437, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789639, Length=255, Percent_Identity=25.8823529411765, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787879, Length=291, Percent_Identity=25.4295532646048, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790783, Length=261, Percent_Identity=25.2873563218391, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34333; Mature: 34333

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNKRHLPSITALQCFEAVTRHLSFTRAAEELNLTQSAVSKQVAQLEELLQHLLFRRVRR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLQMTPAGDLYLVEVRKILTQVEMSTHYLRSYGGETEVLRVSTPSTFGARWLVPRLKGWR
HHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCCCCCE
LRHPSIHLDLCNEQEADDLLQGRSDLAFYFGQGSRPGTECLKLFGEELVPVCAPGSLPDT
ECCCEEEEEECCCCCHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEECCCCCCCC
PLTDPTQLTDLVLLQNASRPQAWHDWFDSQGYQTEHSYHGPRFETFYMCIRAAQVGCGVA
CCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHH
LLPRFLVEEELADGKLVIPWQHAMPSTDAYYLAYPEHAAEVPKVRDFVKWMLEQIDSPDA
HHHHHHHHHHHCCCEEEEEHHHCCCCCCEEEEECCHHHHCCHHHHHHHHHHHHHCCCCCC
V
C
>Mature Secondary Structure
MLNKRHLPSITALQCFEAVTRHLSFTRAAEELNLTQSAVSKQVAQLEELLQHLLFRRVRR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLQMTPAGDLYLVEVRKILTQVEMSTHYLRSYGGETEVLRVSTPSTFGARWLVPRLKGWR
HHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCCCCCE
LRHPSIHLDLCNEQEADDLLQGRSDLAFYFGQGSRPGTECLKLFGEELVPVCAPGSLPDT
ECCCEEEEEECCCCCHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEECCCCCCCC
PLTDPTQLTDLVLLQNASRPQAWHDWFDSQGYQTEHSYHGPRFETFYMCIRAAQVGCGVA
CCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHH
LLPRFLVEEELADGKLVIPWQHAMPSTDAYYLAYPEHAAEVPKVRDFVKWMLEQIDSPDA
HHHHHHHHHHHCCCEEEEEHHHCCCCCCEEEEECCHHHHCCHHHHHHHHHHHHHCCCCCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]