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The map label for this gene is pncB

Identifier: 77456776

GI number: 77456776

Start: 640040

End: 641263

Strand: Reverse

Name: pncB

Synonym: Pfl01_0548

Alternate gene names: 77456776

Gene position: 641263-640040 (Counterclockwise)

Preceding gene: 77456777

Following gene: 77456775

Centisome position: 9.96

GC content: 59.89

Gene sequence:

>1224_bases
ATGAGCGAGAGTGTGTTTGCCGATCGCATCGTGCAGAACCTGCTCGACACCGACTTCTACAAACTGACGATGATGCAGGC
GGTGCTGCACAACTACCCCAACGTCGAAGTCGAATGGGAGTTTCGTTGCCGTAACAGTGAAGATCTGCGCCCGTATCTGG
CGGAGATCCGCTTCCAGGTCGAGCGTCTGGCCGAGTTGAGCCTGAGCGCCGATCAGTTGAGTTTCCTGGAGCGCATCAGC
TTCCTCAAACCTGACTTCCTGCGTTTCCTCGGCCTGTTCCGCTTCAACCTGCGCTACCTGCACACCGGTATCGAGAACGG
CGAGTTGTTCATCCGCCTGCGCGGGCCGTGGCTGCATGTGATTCTGTTCGAAGTGCCGCTGCTGGCGATCGTCAGCGAAG
TGCGCAACCGCTATCGCTACCGTGAAACGGTGCTGGAACAGGCCCGCGAACAGCTGTATCGCAAGTTCGACTGGTTGACC
GCCAATGCCTCGGCCGAAGAGTTGTCGCAGTTGCAGGTCGCCGATTTCGGCACACGCCGGCGGTTTTCCTACCGCGTGCA
GGAAGAAGTGGTGAACGTGCTCAAGCACGATTTCCCCGGACGCTTCGTCGGCACCAGCAACGTGCACCTGTCCCGCGAAC
TGGACATGAAACCGCTGGGCACCATGGCCCACGAGTGGATCATGGCCCACCAGCAACTCGGCCCGCGACTGATCGACAGC
CAGATCGCCGCCCTCGATTGCTGGGTTCGCGAGTACCGCGGCCTGCTGGGGATCGCCCTCACAGACTGCATCACTACCGA
TGCCTTCCTCGGCGATTTCGATCTGTTCTTCGCCAAGCTGTTCGACGGTCTGCGCCACGATTCCGGGGACCCGGTGCTGT
GGGGCGAAAAATGCATCGCCCACTATCACAAGCTCGGCATCGACCCGATGAGCAAGACCCTGGTGTTCTCCGACAGCCTG
ACCCTGCCCAAGTCGCTGGAGATCTTCCGTGCGTTGCGCGGCCGGATCAATGTCAGCTTCGGCATCGGCACCAACCTGAC
CTGTGACATTCCGGGTGTCGAACCGATGAGCATCGTGCTTAAAATGACCGCCTGCAACGGCCAACCGGTGGCGAAGATCT
CCGACGAGCCGGGCAAGACCCACTGCAAAGACCCGAATTTCGTCGCCTATTTGCGACACGTTTTCCAGGTTCCTGCCGTT
TCCAGCCTTTCCAGCAAGGAGTGA

Upstream 100 bases:

>100_bases
TGCCGGGATAAAAATCGCTGGCAATCCCCACCCCGGATATGCGCCACTAGCGCCATTGATTGATACCGCGCCAGAGCGCG
GCCCGCCTGGAGACCCCGTT

Downstream 100 bases:

>100_bases
ATTCATGCAAGCCGTACAGCGTGAGATTGCTGAACAGCTCAACGTTCAGCCGCCGTTCGCCGATGACCAGGCCCTCGAAG
CGGAAGTCGCCCGCCGCATC

Product: nicotinate phosphoribosyltransferase

Products: NA

Alternate protein names: NAPRTase

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSESVFADRIVQNLLDTDFYKLTMMQAVLHNYPNVEVEWEFRCRNSEDLRPYLAEIRFQVERLAELSLSADQLSFLERIS
FLKPDFLRFLGLFRFNLRYLHTGIENGELFIRLRGPWLHVILFEVPLLAIVSEVRNRYRYRETVLEQAREQLYRKFDWLT
ANASAEELSQLQVADFGTRRRFSYRVQEEVVNVLKHDFPGRFVGTSNVHLSRELDMKPLGTMAHEWIMAHQQLGPRLIDS
QIAALDCWVREYRGLLGIALTDCITTDAFLGDFDLFFAKLFDGLRHDSGDPVLWGEKCIAHYHKLGIDPMSKTLVFSDSL
TLPKSLEIFRALRGRINVSFGIGTNLTCDIPGVEPMSIVLKMTACNGQPVAKISDEPGKTHCKDPNFVAYLRHVFQVPAV
SSLSSKE

Sequences:

>Translated_407_residues
MSESVFADRIVQNLLDTDFYKLTMMQAVLHNYPNVEVEWEFRCRNSEDLRPYLAEIRFQVERLAELSLSADQLSFLERIS
FLKPDFLRFLGLFRFNLRYLHTGIENGELFIRLRGPWLHVILFEVPLLAIVSEVRNRYRYRETVLEQAREQLYRKFDWLT
ANASAEELSQLQVADFGTRRRFSYRVQEEVVNVLKHDFPGRFVGTSNVHLSRELDMKPLGTMAHEWIMAHQQLGPRLIDS
QIAALDCWVREYRGLLGIALTDCITTDAFLGDFDLFFAKLFDGLRHDSGDPVLWGEKCIAHYHKLGIDPMSKTLVFSDSL
TLPKSLEIFRALRGRINVSFGIGTNLTCDIPGVEPMSIVLKMTACNGQPVAKISDEPGKTHCKDPNFVAYLRHVFQVPAV
SSLSSKE
>Mature_406_residues
SESVFADRIVQNLLDTDFYKLTMMQAVLHNYPNVEVEWEFRCRNSEDLRPYLAEIRFQVERLAELSLSADQLSFLERISF
LKPDFLRFLGLFRFNLRYLHTGIENGELFIRLRGPWLHVILFEVPLLAIVSEVRNRYRYRETVLEQAREQLYRKFDWLTA
NASAEELSQLQVADFGTRRRFSYRVQEEVVNVLKHDFPGRFVGTSNVHLSRELDMKPLGTMAHEWIMAHQQLGPRLIDSQ
IAALDCWVREYRGLLGIALTDCITTDAFLGDFDLFFAKLFDGLRHDSGDPVLWGEKCIAHYHKLGIDPMSKTLVFSDSLT
LPKSLEIFRALRGRINVSFGIGTNLTCDIPGVEPMSIVLKMTACNGQPVAKISDEPGKTHCKDPNFVAYLRHVFQVPAVS
SLSSKE

Specific function: NAD biosynthesis; nicotinamide to NaMN; second step. [C]

COG id: COG1488

COG function: function code H; Nicotinic acid phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAPRTase family

Homologues:

Organism=Escherichia coli, GI1787162, Length=399, Percent_Identity=52.6315789473684, Blast_Score=414, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6324783, Length=416, Percent_Identity=30.2884615384615, Blast_Score=195, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PNCB_PSEPF (Q3KIW4)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_346281.1
- HSSP:   P39683
- ProteinModelPortal:   Q3KIW4
- SMR:   Q3KIW4
- STRING:   Q3KIW4
- GeneID:   3712943
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_0548
- eggNOG:   COG1488
- HOGENOM:   HBG325503
- OMA:   HYRANRI
- ProtClustDB:   PRK05321
- BioCyc:   PFLU205922:PFL_0548-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00570
- InterPro:   IPR006406
- InterPro:   IPR015977
- InterPro:   IPR007229
- InterPro:   IPR002638
- PANTHER:   PTHR11098:SF1
- PIRSF:   PIRSF000484
- TIGRFAMs:   TIGR01514

Pfam domain/function: PF04095 NAPRTase; SSF51690 Q_phspho_trans

EC number: =2.4.2.11

Molecular weight: Translated: 46873; Mature: 46741

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESVFADRIVQNLLDTDFYKLTMMQAVLHNYPNVEVEWEFRCRNSEDLRPYLAEIRFQV
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
ERLAELSLSADQLSFLERISFLKPDFLRFLGLFRFNLRYLHTGIENGELFIRLRGPWLHV
HHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHH
ILFEVPLLAIVSEVRNRYRYRETVLEQAREQLYRKFDWLTANASAEELSQLQVADFGTRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCH
RFSYRVQEEVVNVLKHDFPGRFVGTSNVHLSRELDMKPLGTMAHEWIMAHQQLGPRLIDS
HHHHHHHHHHHHHHHHCCCCCEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHH
QIAALDCWVREYRGLLGIALTDCITTDAFLGDFDLFFAKLFDGLRHDSGDPVLWGEKCIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHH
HYHKLGIDPMSKTLVFSDSLTLPKSLEIFRALRGRINVSFGIGTNLTCDIPGVEPMSIVL
HHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCEECCCCCCHHEEEE
KMTACNGQPVAKISDEPGKTHCKDPNFVAYLRHVFQVPAVSSLSSKE
EEECCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SESVFADRIVQNLLDTDFYKLTMMQAVLHNYPNVEVEWEFRCRNSEDLRPYLAEIRFQV
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
ERLAELSLSADQLSFLERISFLKPDFLRFLGLFRFNLRYLHTGIENGELFIRLRGPWLHV
HHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHH
ILFEVPLLAIVSEVRNRYRYRETVLEQAREQLYRKFDWLTANASAEELSQLQVADFGTRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCH
RFSYRVQEEVVNVLKHDFPGRFVGTSNVHLSRELDMKPLGTMAHEWIMAHQQLGPRLIDS
HHHHHHHHHHHHHHHHCCCCCEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHH
QIAALDCWVREYRGLLGIALTDCITTDAFLGDFDLFFAKLFDGLRHDSGDPVLWGEKCIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHH
HYHKLGIDPMSKTLVFSDSLTLPKSLEIFRALRGRINVSFGIGTNLTCDIPGVEPMSIVL
HHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCEECCCCCCHHEEEE
KMTACNGQPVAKISDEPGKTHCKDPNFVAYLRHVFQVPAVSSLSSKE
EEECCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA