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The map label for this gene is phaB [H]

Identifier: 77456621

GI number: 77456621

Start: 445947

End: 446801

Strand: Reverse

Name: phaB [H]

Synonym: Pfl01_0393

Alternate gene names: 77456621

Gene position: 446801-445947 (Counterclockwise)

Preceding gene: 77456622

Following gene: 77456620

Centisome position: 6.94

GC content: 64.33

Gene sequence:

>855_bases
ATGCCGCACCCGTTCATCTTTCGAACTGTCGAGCTGGATGGCCAGACCATCCGCACGGCGGTCCGCCCCGGCAAGCCTCA
CTTGACGCCCTTGCTGATATTCAACGGCATCGGCGCCAACCTGGAGCTGGTGTTTCCTTTTGTCGCGGCGCTGGATCCGG
ATCTGGAAGTGATCGCCTTCGACGTGCCCGGTGTCGGCGGTTCCTCGACGCCGAGCCGGCCGTATCGCTTTCCGGGGCTG
GCGAAGCTCACCGCGCGGATGCTCGATTACCTCGACTACGGACAGGTCAACGTGATCGGCGTGTCCTGGGGTGGCGCGCT
GGCGCAACAGTTCGCCTACGACTATCCCGAGCGCTGCAAGAAGCTGGTGCTGGCGGCGACCGCTGCCGGTGCGGTGATGG
TGCCTGGCAAGCCGAAAGTGCTGTGGATGATGGCCAGCCCACGGCGCTACATCCAGCCGTCACATGTGATCCGCATTGCC
CCGATGATCTACGGCGGCTCGTTCCGCCGCGACCCGACACTCGCCGCCAGCCATGCGGCCAAGGTGCGCTCGGCGGGCAA
GCTCGGTTACTACTGGCAACTGTTCGCGGGCCTGGGCTGGACCAGCATTCACTGGCTGCACAAGATCCACCAGCCGACCC
TGGTGCTGGCCGGTGATGACGATCCGCTGATCCCGCTGATCAACATGCGCATGCTCGCCTGGCGAATCCCCAACGCGCAG
CTGCACATCATCGACGACGGGCATCTGTTCCTGATTACCAGGGCCGAGGCGGTGGCGCCGATCATCATGAAATTCCTCCA
GGAGGAACGTCAGCGTGCGGTGATGCATCCGCACCCGACACCGCTGGGCGGATAA

Upstream 100 bases:

>100_bases
CTTCACCGGGCACTTACGTTCACGAGCGGTAACCGACACCCCTCACTCCCTGCGGGGAGTGAGGTGATCACTGAGATTGC
GACCCACAGGGCCTTGAAGC

Downstream 100 bases:

>100_bases
CCGTCCCGGCACGGATTTCTGGACAGCGTTTTGCAGCGCGTCTGGACAGGTGCGTGGCAGGAGGCCGCGCAAGCAGCAAC
CCGCAACAGCGTCTATGGTG

Product: Poly(3-hydroxyalkanoate) depolymerase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MPHPFIFRTVELDGQTIRTAVRPGKPHLTPLLIFNGIGANLELVFPFVAALDPDLEVIAFDVPGVGGSSTPSRPYRFPGL
AKLTARMLDYLDYGQVNVIGVSWGGALAQQFAYDYPERCKKLVLAATAAGAVMVPGKPKVLWMMASPRRYIQPSHVIRIA
PMIYGGSFRRDPTLAASHAAKVRSAGKLGYYWQLFAGLGWTSIHWLHKIHQPTLVLAGDDDPLIPLINMRMLAWRIPNAQ
LHIIDDGHLFLITRAEAVAPIIMKFLQEERQRAVMHPHPTPLGG

Sequences:

>Translated_284_residues
MPHPFIFRTVELDGQTIRTAVRPGKPHLTPLLIFNGIGANLELVFPFVAALDPDLEVIAFDVPGVGGSSTPSRPYRFPGL
AKLTARMLDYLDYGQVNVIGVSWGGALAQQFAYDYPERCKKLVLAATAAGAVMVPGKPKVLWMMASPRRYIQPSHVIRIA
PMIYGGSFRRDPTLAASHAAKVRSAGKLGYYWQLFAGLGWTSIHWLHKIHQPTLVLAGDDDPLIPLINMRMLAWRIPNAQ
LHIIDDGHLFLITRAEAVAPIIMKFLQEERQRAVMHPHPTPLGG
>Mature_283_residues
PHPFIFRTVELDGQTIRTAVRPGKPHLTPLLIFNGIGANLELVFPFVAALDPDLEVIAFDVPGVGGSSTPSRPYRFPGLA
KLTARMLDYLDYGQVNVIGVSWGGALAQQFAYDYPERCKKLVLAATAAGAVMVPGKPKVLWMMASPRRYIQPSHVIRIAP
MIYGGSFRRDPTLAASHAAKVRSAGKLGYYWQLFAGLGWTSIHWLHKIHQPTLVLAGDDDPLIPLINMRMLAWRIPNAQL
HIIDDGHLFLITRAEAVAPIIMKFLQEERQRAVMHPHPTPLGG

Specific function: PHA depolymerase is in fact a lipase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR011942 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 31404; Mature: 31273

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHPFIFRTVELDGQTIRTAVRPGKPHLTPLLIFNGIGANLELVFPFVAALDPDLEVIAF
CCCCEEEEEEEECCCEEEEECCCCCCCCEEEEEEECCCCCEEEEEEHHHHCCCCCEEEEE
DVPGVGGSSTPSRPYRFPGLAKLTARMLDYLDYGQVNVIGVSWGGALAQQFAYDYPERCK
ECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHCCCCHHHHH
KLVLAATAAGAVMVPGKPKVLWMMASPRRYIQPSHVIRIAPMIYGGSFRRDPTLAASHAA
HHHHHEECCCEEEECCCCCEEEEEECCHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHHH
KVRSAGKLGYYWQLFAGLGWTSIHWLHKIHQPTLVLAGDDDPLIPLINMRMLAWRIPNAQ
HHHCCCCCCHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHCCEEEEEECCCCE
LHIIDDGHLFLITRAEAVAPIIMKFLQEERQRAVMHPHPTPLGG
EEEEECCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PHPFIFRTVELDGQTIRTAVRPGKPHLTPLLIFNGIGANLELVFPFVAALDPDLEVIAF
CCCEEEEEEEECCCEEEEECCCCCCCCEEEEEEECCCCCEEEEEEHHHHCCCCCEEEEE
DVPGVGGSSTPSRPYRFPGLAKLTARMLDYLDYGQVNVIGVSWGGALAQQFAYDYPERCK
ECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHCCCCHHHHH
KLVLAATAAGAVMVPGKPKVLWMMASPRRYIQPSHVIRIAPMIYGGSFRRDPTLAASHAA
HHHHHEECCCEEEECCCCCEEEEEECCHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHHH
KVRSAGKLGYYWQLFAGLGWTSIHWLHKIHQPTLVLAGDDDPLIPLINMRMLAWRIPNAQ
HHHCCCCCCHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHCCEEEEEECCCCE
LHIIDDGHLFLITRAEAVAPIIMKFLQEERQRAVMHPHPTPLGG
EEEEECCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1989978 [H]