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The map label for this gene is phaA [H]

Identifier: 77456622

GI number: 77456622

Start: 446870

End: 448549

Strand: Reverse

Name: phaA [H]

Synonym: Pfl01_0394

Alternate gene names: 77456622

Gene position: 448549-446870 (Counterclockwise)

Preceding gene: 77456623

Following gene: 77456621

Centisome position: 6.97

GC content: 60.6

Gene sequence:

>1680_bases
ATGAGTAACAAGAATAACGATGATTTGAAGTACCAGGCCTCGGAGAACACCCTGGGGCTGAATCCTGTCGTTGGGCTACG
CGGAAAGGATCTGCTGGCCTCTGCTCGAATGGTGCTGACCCAGGCCATCAAACAACCGATCCATAGTGTCAAACACGTCA
CCCAGTTCGGCCTCGAACTGAAGAACGTGCTGTTCGGCAAATCCGACCTGCAACCGGCGGGCGATGACCGTCGCTTCGTC
GATCCGGCGTGGAGTCAGAACCCGCTGTACAAACGTTATCTGCAAACCTATCTGGCGTGGCGCAAGGAACTCCATGCCTG
GATCGACGACAGCAGTCTCTCGCCCAAGGACATCGCGCGCGGCCACTTCGTGATCAACCTGATGACCGAGGCCATGGCCC
CGACCAACACGGCGGCCAACCCTGCGGCGGTCAAACGTTTCTTCGAGACCGGCGGCAAGAGCCTGCTCGACGGCCTCTCG
CACCTGGCCAAGGATCTGGTGCACAACGGCGGCATGCCGAGCCAGGTCAACATGGGCGCGTTCGAGGTCGGCAAGAGCCT
GGGCGTGACCGAAGGCGCGGTGGTGTTTCGCAACGATGTGCTGGAGCTGATCCAGTACAAGCCGATCACCGAGCAAGTGC
ACGAGCGCCCGCTGCTCGTCGTGCCGCCGCAGATCAACAAGTTCTATGTATTTGACCTGAGCCCGGACAAGAGCCTGGCG
CGTTTCTGCCTGCGCAACAATGTGCAGACCTTCATCGTCAGCTGGCGCAACCCGACCAAGGAACAGCGCGAGTGGGGCCT
CTCGACCTACATCGAAGCCCTGAAGGAAGCGGTCGACGTGGTCACCGCGATCACCGGCAGCAAGGACGTCAACATGCTTG
GCGCCTGCTCCGGCGGCATCACCTGTACCGCCCTGCTCGGCCACTATGCCGCCACGGGCGAGAACAAGGTCAATGCCCTG
ACCCTGCTGGTCAGCGTGCTCGACACCACCCTCGACAGTGATGTCGCACTGTTCGTCGACGAGCAGACGCTGGAAATGGC
CAAGCGACACTCCTATCAGGCCGGCGTGCTCGAAGGCAAAGACATGGCCAAAGTCTTTGCCTGGATGCGTCCCAACGATC
TGATCTGGAACTACTGGGTCAACAACTACCTGCTCGGCAACGAGCCGCCGGTGTTCGACATCCTGTTCTGGAACAACGAT
ACGACTCGACTGCCGGCCGCGTTCCACGGCGACCTGATCGAGATGTTCAAAAACAACCCACTGATCCGCCCCAATGCACT
GGAAGTGTGCGGCACGCCGATCGACCTCAAGCAGGTGACCGCCGACATCTTCTCGCTGGCCGGCACCAACGACCACATCA
CGCCGTGGAAGTCCTGCTACAAGTCGGCGCAGCTGTTTGGTGGCAAGGTCGAATTCGTGCTGTCCAGCAGCGGACATATC
CAGAGCATCCTGAACCCGCCGGGCAACCCGAAATCGCGCTACATGACCAGCGATGAAATGACCGCCAATGCCGATGACTG
GCAGGAAAACTCGACCAAGCACGCCGACTCCTGGTGGCTGTACTGGCAGGCGTGGCAGGCCGCGCGCTCGGGCGAGCTGA
AAAAGGCGCCGACCAAGCTGGGCAACAAGGCGTATCCGGCAGGCGAAGCTTCACCGGGCACTTACGTTCACGAGCGGTAA

Upstream 100 bases:

>100_bases
TGCAGAATCAACGGTCACCCGAGCAGTAGTACCTGGCATTGGCTGTGGAACTGCACAGCAGAAAACCGGGTACTCGTCTC
AGGACAATGGAGCGTCGTAG

Downstream 100 bases:

>100_bases
CCGACACCCCTCACTCCCTGCGGGGAGTGAGGTGATCACTGAGATTGCGACCCACAGGGCCTTGAAGCATGCCGCACCCG
TTCATCTTTCGAACTGTCGA

Product: Poly(R)-hydroxyalkanoic acid synthase, class II

Products: NA

Alternate protein names: PHA polymerase 1; PHA synthase 1; Polyhydroxyalkanoic acid synthase 1 [H]

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MSNKNNDDLKYQASENTLGLNPVVGLRGKDLLASARMVLTQAIKQPIHSVKHVTQFGLELKNVLFGKSDLQPAGDDRRFV
DPAWSQNPLYKRYLQTYLAWRKELHAWIDDSSLSPKDIARGHFVINLMTEAMAPTNTAANPAAVKRFFETGGKSLLDGLS
HLAKDLVHNGGMPSQVNMGAFEVGKSLGVTEGAVVFRNDVLELIQYKPITEQVHERPLLVVPPQINKFYVFDLSPDKSLA
RFCLRNNVQTFIVSWRNPTKEQREWGLSTYIEALKEAVDVVTAITGSKDVNMLGACSGGITCTALLGHYAATGENKVNAL
TLLVSVLDTTLDSDVALFVDEQTLEMAKRHSYQAGVLEGKDMAKVFAWMRPNDLIWNYWVNNYLLGNEPPVFDILFWNND
TTRLPAAFHGDLIEMFKNNPLIRPNALEVCGTPIDLKQVTADIFSLAGTNDHITPWKSCYKSAQLFGGKVEFVLSSSGHI
QSILNPPGNPKSRYMTSDEMTANADDWQENSTKHADSWWLYWQAWQAARSGELKKAPTKLGNKAYPAGEASPGTYVHER

Sequences:

>Translated_559_residues
MSNKNNDDLKYQASENTLGLNPVVGLRGKDLLASARMVLTQAIKQPIHSVKHVTQFGLELKNVLFGKSDLQPAGDDRRFV
DPAWSQNPLYKRYLQTYLAWRKELHAWIDDSSLSPKDIARGHFVINLMTEAMAPTNTAANPAAVKRFFETGGKSLLDGLS
HLAKDLVHNGGMPSQVNMGAFEVGKSLGVTEGAVVFRNDVLELIQYKPITEQVHERPLLVVPPQINKFYVFDLSPDKSLA
RFCLRNNVQTFIVSWRNPTKEQREWGLSTYIEALKEAVDVVTAITGSKDVNMLGACSGGITCTALLGHYAATGENKVNAL
TLLVSVLDTTLDSDVALFVDEQTLEMAKRHSYQAGVLEGKDMAKVFAWMRPNDLIWNYWVNNYLLGNEPPVFDILFWNND
TTRLPAAFHGDLIEMFKNNPLIRPNALEVCGTPIDLKQVTADIFSLAGTNDHITPWKSCYKSAQLFGGKVEFVLSSSGHI
QSILNPPGNPKSRYMTSDEMTANADDWQENSTKHADSWWLYWQAWQAARSGELKKAPTKLGNKAYPAGEASPGTYVHER
>Mature_558_residues
SNKNNDDLKYQASENTLGLNPVVGLRGKDLLASARMVLTQAIKQPIHSVKHVTQFGLELKNVLFGKSDLQPAGDDRRFVD
PAWSQNPLYKRYLQTYLAWRKELHAWIDDSSLSPKDIARGHFVINLMTEAMAPTNTAANPAAVKRFFETGGKSLLDGLSH
LAKDLVHNGGMPSQVNMGAFEVGKSLGVTEGAVVFRNDVLELIQYKPITEQVHERPLLVVPPQINKFYVFDLSPDKSLAR
FCLRNNVQTFIVSWRNPTKEQREWGLSTYIEALKEAVDVVTAITGSKDVNMLGACSGGITCTALLGHYAATGENKVNALT
LLVSVLDTTLDSDVALFVDEQTLEMAKRHSYQAGVLEGKDMAKVFAWMRPNDLIWNYWVNNYLLGNEPPVFDILFWNNDT
TRLPAAFHGDLIEMFKNNPLIRPNALEVCGTPIDLKQVTADIFSLAGTNDHITPWKSCYKSAQLFGGKVEFVLSSSGHIQ
SILNPPGNPKSRYMTSDEMTANADDWQENSTKHADSWWLYWQAWQAARSGELKKAPTKLGNKAYPAGEASPGTYVHER

Specific function: P.oleovorans accumulates poly(3-hydroxyalkanoates) after growth on medium chain length hydrocarbons. Large amounts of this polyester are synthesized when cells are grown under nitrogen- limiting conditions. When nitrogen is resupplied in the medium, the a

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR011287
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 62244; Mature: 62113

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKNNDDLKYQASENTLGLNPVVGLRGKDLLASARMVLTQAIKQPIHSVKHVTQFGLEL
CCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNVLFGKSDLQPAGDDRRFVDPAWSQNPLYKRYLQTYLAWRKELHAWIDDSSLSPKDIAR
HHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHC
GHFVINLMTEAMAPTNTAANPAAVKRFFETGGKSLLDGLSHLAKDLVHNGGMPSQVNMGA
CHHEEEEHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCH
FEVGKSLGVTEGAVVFRNDVLELIQYKPITEQVHERPLLVVPPQINKFYVFDLSPDKSLA
HHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCEEEECCCCCEEEEEECCCCHHHH
RFCLRNNVQTFIVSWRNPTKEQREWGLSTYIEALKEAVDVVTAITGSKDVNMLGACSGGI
HHHHHCCCEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCC
TCTALLGHYAATGENKVNALTLLVSVLDTTLDSDVALFVDEQTLEMAKRHSYQAGVLEGK
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCCCCCH
DMAKVFAWMRPNDLIWNYWVNNYLLGNEPPVFDILFWNNDTTRLPAAFHGDLIEMFKNNP
HHHHHHHHCCCCCEEEEEECCCEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCC
LIRPNALEVCGTPIDLKQVTADIFSLAGTNDHITPWKSCYKSAQLFGGKVEFVLSSSGHI
CCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCH
QSILNPPGNPKSRYMTSDEMTANADDWQENSTKHADSWWLYWQAWQAARSGELKKAPTKL
HHHCCCCCCCHHCEECCCCCCCCCCCHHCCCCCCCCCEEEHHHHHHHHHCCCCHHHHHHC
GNKAYPAGEASPGTYVHER
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNKNNDDLKYQASENTLGLNPVVGLRGKDLLASARMVLTQAIKQPIHSVKHVTQFGLEL
CCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNVLFGKSDLQPAGDDRRFVDPAWSQNPLYKRYLQTYLAWRKELHAWIDDSSLSPKDIAR
HHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHC
GHFVINLMTEAMAPTNTAANPAAVKRFFETGGKSLLDGLSHLAKDLVHNGGMPSQVNMGA
CHHEEEEHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCH
FEVGKSLGVTEGAVVFRNDVLELIQYKPITEQVHERPLLVVPPQINKFYVFDLSPDKSLA
HHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCEEEECCCCCEEEEEECCCCHHHH
RFCLRNNVQTFIVSWRNPTKEQREWGLSTYIEALKEAVDVVTAITGSKDVNMLGACSGGI
HHHHHCCCEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCC
TCTALLGHYAATGENKVNALTLLVSVLDTTLDSDVALFVDEQTLEMAKRHSYQAGVLEGK
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCCCCCH
DMAKVFAWMRPNDLIWNYWVNNYLLGNEPPVFDILFWNNDTTRLPAAFHGDLIEMFKNNP
HHHHHHHHCCCCCEEEEEECCCEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCC
LIRPNALEVCGTPIDLKQVTADIFSLAGTNDHITPWKSCYKSAQLFGGKVEFVLSSSGHI
CCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCH
QSILNPPGNPKSRYMTSDEMTANADDWQENSTKHADSWWLYWQAWQAARSGELKKAPTKL
HHHCCCCCCCHHCEECCCCCCCCCCCHHCCCCCCCCCEEEHHHHHHHHHCCCCHHHHHHC
GNKAYPAGEASPGTYVHER
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1989978 [H]