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The map label for this gene is mdtE [H]

Identifier: 77456458

GI number: 77456458

Start: 270195

End: 271298

Strand: Direct

Name: mdtE [H]

Synonym: Pfl01_0230

Alternate gene names: 77456458

Gene position: 270195-271298 (Clockwise)

Preceding gene: 77456456

Following gene: 77456459

Centisome position: 4.2

GC content: 62.95

Gene sequence:

>1104_bases
ATGGCGAGTCCCGGATTGAAAACAGCGGTCATGCTCAGTCTGTTCGCTTTATTGAGCGCCTGCGGCGAGAAAAAGGCCCC
TGCGGAATACCTGCCCCGGGTCTTCGTGCAGGAGGTGAACCCCGCCAATTACGCCGCCGCCGTGACCCTCACCGGCGATG
TTCAGGCACGGGTGCAGACCGAACTGTCGTTCCGGGTCGGCGGCAAGATCATCCAGCGCATGGTCGATGTCGGTGACCGT
GTCTCGGCCAAACAAGTGCTGGCCAAGCTCGATCCGAAGGATTTGCAGACCAATGTCGATTCCGCTCAGGCTCAGGTAGT
GGCCGAGCAGGCGCGGGTCAAACAGAGCGCCGCCGCGTTCGTCCGCCAGCAGAAACTGTTGCCCAAGGGCTACACCAGCC
AGAGCGAATACGATTCCGCGCAGGCTGCATTGCGCAGCAGCCAGAGTGCCTTGAGTGCAGCGCAGGCGCAGTTGGCCAAC
GCCAAGGATCAATTGAGCTATACCGCGCTGATCGCCGATGCGCCCGGCGTCATCACCGAGCGTCAGGCCGAAGTCGGCCA
GGTGGTTCAGGCCACGGCACCGATTTTCAGCCTGGCCCGGGACGGCGACCGCGACGCCGTGTTCAACGTCTACGAATCCC
TGCTGGCCGAACGGCCGGCGGAGCGTTCGATTGTCGTCAGCCTGCTCGACAACCCCGCCATCAAGACCACCGGCACCGTG
CGCGAAATCACCCCGGCGGTGTCCGCGCAGTCCGGCACGGTGCAGGTCAAAGTCAGCCTCGACAGCCTGCCGCAAGGGAT
GCAACTGGGCTCGGTGGTGAGCGCCACGGCCAAGGGTACCGGCAAATCGGCCGTTGAATTGCCGTGGTCGGCGCTGACCA
AGAACGTCAGCGATCCGGCGGTGTGGATGGTCGACGACAAAGGTGAAGCGCAGTTGCACAACGTCACCGTCAGCCGCTAC
CTGATCGGCAAGGTCATCATCAGCGAGGGCCTCAAGGGCGGGGAAAAAGTCATCGTCGCGGGTGGGCAGTTGCTGCATCC
GGGCATGAAGGTCGAAATTGCCGAAAACACCTACAAGGATCTGCAGCCGGGAGCACAGCCATGA

Upstream 100 bases:

>100_bases
CCGCACAGAAATGCATATATCGCCCGGACGAAGGTTGAACCCTGTTCTAAGCTCACAGTCGTATCGTCACTTGCACGTTC
GTACACAGGAAGGAGTCTGC

Downstream 100 bases:

>100_bases
AGCGTCTGGGGCTGTTATCCATTGGCGTGTTGCTGGCCGCCTGCTCGAAAAGCGAGCCACCGCCGGAGCCGGTACGGCCG
GTGTTGTCGGTCAAGGTCGA

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MASPGLKTAVMLSLFALLSACGEKKAPAEYLPRVFVQEVNPANYAAAVTLTGDVQARVQTELSFRVGGKIIQRMVDVGDR
VSAKQVLAKLDPKDLQTNVDSAQAQVVAEQARVKQSAAAFVRQQKLLPKGYTSQSEYDSAQAALRSSQSALSAAQAQLAN
AKDQLSYTALIADAPGVITERQAEVGQVVQATAPIFSLARDGDRDAVFNVYESLLAERPAERSIVVSLLDNPAIKTTGTV
REITPAVSAQSGTVQVKVSLDSLPQGMQLGSVVSATAKGTGKSAVELPWSALTKNVSDPAVWMVDDKGEAQLHNVTVSRY
LIGKVIISEGLKGGEKVIVAGGQLLHPGMKVEIAENTYKDLQPGAQP

Sequences:

>Translated_367_residues
MASPGLKTAVMLSLFALLSACGEKKAPAEYLPRVFVQEVNPANYAAAVTLTGDVQARVQTELSFRVGGKIIQRMVDVGDR
VSAKQVLAKLDPKDLQTNVDSAQAQVVAEQARVKQSAAAFVRQQKLLPKGYTSQSEYDSAQAALRSSQSALSAAQAQLAN
AKDQLSYTALIADAPGVITERQAEVGQVVQATAPIFSLARDGDRDAVFNVYESLLAERPAERSIVVSLLDNPAIKTTGTV
REITPAVSAQSGTVQVKVSLDSLPQGMQLGSVVSATAKGTGKSAVELPWSALTKNVSDPAVWMVDDKGEAQLHNVTVSRY
LIGKVIISEGLKGGEKVIVAGGQLLHPGMKVEIAENTYKDLQPGAQP
>Mature_366_residues
ASPGLKTAVMLSLFALLSACGEKKAPAEYLPRVFVQEVNPANYAAAVTLTGDVQARVQTELSFRVGGKIIQRMVDVGDRV
SAKQVLAKLDPKDLQTNVDSAQAQVVAEQARVKQSAAAFVRQQKLLPKGYTSQSEYDSAQAALRSSQSALSAAQAQLANA
KDQLSYTALIADAPGVITERQAEVGQVVQATAPIFSLARDGDRDAVFNVYESLLAERPAERSIVVSLLDNPAIKTTGTVR
EITPAVSAQSGTVQVKVSLDSLPQGMQLGSVVSATAKGTGKSAVELPWSALTKNVSDPAVWMVDDKGEAQLHNVTVSRYL
IGKVIISEGLKGGEKVIVAGGQLLHPGMKVEIAENTYKDLQPGAQP

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=362, Percent_Identity=25.6906077348066, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87082036, Length=300, Percent_Identity=26.6666666666667, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789665, Length=363, Percent_Identity=26.1707988980716, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786668, Length=387, Percent_Identity=27.6485788113695, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87081796, Length=327, Percent_Identity=24.7706422018349, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38763; Mature: 38631

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASPGLKTAVMLSLFALLSACGEKKAPAEYLPRVFVQEVNPANYAAAVTLTGDVQARVQT
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCEEEHHH
ELSFRVGGKIIQRMVDVGDRVSAKQVLAKLDPKDLQTNVDSAQAQVVAEQARVKQSAAAF
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VRQQKLLPKGYTSQSEYDSAQAALRSSQSALSAAQAQLANAKDQLSYTALIADAPGVITE
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHEEEEEEECCCCCCCH
RQAEVGQVVQATAPIFSLARDGDRDAVFNVYESLLAERPAERSIVVSLLDNPAIKTTGTV
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHEEEEECCCCCCCCCCCH
REITPAVSAQSGTVQVKVSLDSLPQGMQLGSVVSATAKGTGKSAVELPWSALTKNVSDPA
HHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCEECCHHHHHCCCCCCE
VWMVDDKGEAQLHNVTVSRYLIGKVIISEGLKGGEKVIVAGGQLLHPGMKVEIAENTYKD
EEEEECCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEECCCCEEEECCCHHHH
LQPGAQP
CCCCCCC
>Mature Secondary Structure 
ASPGLKTAVMLSLFALLSACGEKKAPAEYLPRVFVQEVNPANYAAAVTLTGDVQARVQT
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCEEEHHH
ELSFRVGGKIIQRMVDVGDRVSAKQVLAKLDPKDLQTNVDSAQAQVVAEQARVKQSAAAF
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VRQQKLLPKGYTSQSEYDSAQAALRSSQSALSAAQAQLANAKDQLSYTALIADAPGVITE
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHEEEEEEECCCCCCCH
RQAEVGQVVQATAPIFSLARDGDRDAVFNVYESLLAERPAERSIVVSLLDNPAIKTTGTV
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHEEEEECCCCCCCCCCCH
REITPAVSAQSGTVQVKVSLDSLPQGMQLGSVVSATAKGTGKSAVELPWSALTKNVSDPA
HHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCEECCHHHHHCCCCCCE
VWMVDDKGEAQLHNVTVSRYLIGKVIISEGLKGGEKVIVAGGQLLHPGMKVEIAENTYKD
EEEEECCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEECCCCEEEECCCHHHH
LQPGAQP
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]