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The map label for this gene is cysW [H]

Identifier: 77456424

GI number: 77456424

Start: 236409

End: 237281

Strand: Direct

Name: cysW [H]

Synonym: Pfl01_0196

Alternate gene names: 77456424

Gene position: 236409-237281 (Clockwise)

Preceding gene: 77456423

Following gene: 77456425

Centisome position: 3.67

GC content: 62.2

Gene sequence:

>873_bases
ATGTCCCAATCGTCTATTGCGGCCGCGTCCTCGGCCAACGCCGCCCGCCGTGGCAGCGCCACTTCGCGGCGGATCCTGAT
CGGCCTCGGCTGGCTGATTTTCTTTCTGTTTCTGCTGCTGCCGCTGTTCATCGTGGTGTCGCAGGGATTGAAGAATGGTC
TCGGCGCGTTCTTCACCGCGATCTTTGAACCGGATGCCTTGTCGGCGCTGAAACTCACCGTGATTGCGGTGCTGGTGTCG
GTGCCGCTGAACCTGGTGTTCGGTGTCAGCGCCGCGTGGTGCGTGAGCAAATACTCGTTCCGCGGCAAGAGTATGCTGGT
GACGCTGATCGACCTGCCGTTCTCGGTGTCGCCGGTGATCGCCGGTCTGGTCTACGTGTTGATGTTCGGTGCCCAGGGCC
TGTTCGGGCCGTGGCTGCAGGATCATGACATCCAGATCGTCTTCGCCCTGCCGGGCATCGTGCTGGCGACGATCTTCGTC
ACCGTGCCATTCGTGGCCCGTGAGCTGATCCCGCTGATGCAGGAACAAGGCACTCAGGAAGAGGAAGCCGCGCGCCTGCT
CGGCGCCAATGGCTGGCAGATGTTCTGGCACGTGACCGTCCCCAACATCAAATGGGGTCTGATCTATGGCGTGGTGCTGT
GTACCGCGCGGGCCATGGGTGAGTTCGGTGCGGTGTCGGTGGTTTCCGGGCACATTCGCGGGGTGACCAACACCTTGCCG
CTGCACGTCGAGATCCTCTACAACGAATACAACCACGTGGCCGCGTTCGCCGTGGCGAGCCTGTTGCTGATCCTGGCGCT
CTTCATCCTGCTGCTCAAGCAGTGGAGCGAAAACCGTATCAACCGCCTGCGCGCCAGCGCCGCGGAGGAATAA

Upstream 100 bases:

>100_bases
ACCTCCATCGGCGTATTGATGCTGGTGGTTTCCTTCGTCCTGTTGCTGCTGATCAACTTGCTGCAGCGGCGCATCGAAAC
CCCATAAGGAGGCGCGAACC

Downstream 100 bases:

>100_bases
TTCATGTCGATCGAAGTGCGTAACGTCAGCAAGAATTTCAATGCGTTCAAGGCGCTGGACAACATCAGTCTGGACATCCA
CAGCGGTGAACTGGTGGCAT

Product: Sulfate ABC transporter, permease CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSQSSIAAASSANAARRGSATSRRILIGLGWLIFFLFLLLPLFIVVSQGLKNGLGAFFTAIFEPDALSALKLTVIAVLVS
VPLNLVFGVSAAWCVSKYSFRGKSMLVTLIDLPFSVSPVIAGLVYVLMFGAQGLFGPWLQDHDIQIVFALPGIVLATIFV
TVPFVARELIPLMQEQGTQEEEAARLLGANGWQMFWHVTVPNIKWGLIYGVVLCTARAMGEFGAVSVVSGHIRGVTNTLP
LHVEILYNEYNHVAAFAVASLLLILALFILLLKQWSENRINRLRASAAEE

Sequences:

>Translated_290_residues
MSQSSIAAASSANAARRGSATSRRILIGLGWLIFFLFLLLPLFIVVSQGLKNGLGAFFTAIFEPDALSALKLTVIAVLVS
VPLNLVFGVSAAWCVSKYSFRGKSMLVTLIDLPFSVSPVIAGLVYVLMFGAQGLFGPWLQDHDIQIVFALPGIVLATIFV
TVPFVARELIPLMQEQGTQEEEAARLLGANGWQMFWHVTVPNIKWGLIYGVVLCTARAMGEFGAVSVVSGHIRGVTNTLP
LHVEILYNEYNHVAAFAVASLLLILALFILLLKQWSENRINRLRASAAEE
>Mature_289_residues
SQSSIAAASSANAARRGSATSRRILIGLGWLIFFLFLLLPLFIVVSQGLKNGLGAFFTAIFEPDALSALKLTVIAVLVSV
PLNLVFGVSAAWCVSKYSFRGKSMLVTLIDLPFSVSPVIAGLVYVLMFGAQGLFGPWLQDHDIQIVFALPGIVLATIFVT
VPFVARELIPLMQEQGTQEEEAARLLGANGWQMFWHVTVPNIKWGLIYGVVLCTARAMGEFGAVSVVSGHIRGVTNTLPL
HVEILYNEYNHVAAFAVASLLLILALFILLLKQWSENRINRLRASAAEE

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=269, Percent_Identity=52.4163568773234, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1788764, Length=206, Percent_Identity=36.4077669902913, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1786980, Length=221, Percent_Identity=33.9366515837104, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787369, Length=271, Percent_Identity=26.9372693726937, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31515; Mature: 31383

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSSIAAASSANAARRGSATSRRILIGLGWLIFFLFLLLPLFIVVSQGLKNGLGAFFTA
CCCCHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFEPDALSALKLTVIAVLVSVPLNLVFGVSAAWCVSKYSFRGKSMLVTLIDLPFSVSPVI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
AGLVYVLMFGAQGLFGPWLQDHDIQIVFALPGIVLATIFVTVPFVARELIPLMQEQGTQE
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EEAARLLGANGWQMFWHVTVPNIKWGLIYGVVLCTARAMGEFGAVSVVSGHIRGVTNTLP
HHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
LHVEILYNEYNHVAAFAVASLLLILALFILLLKQWSENRINRLRASAAEE
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SQSSIAAASSANAARRGSATSRRILIGLGWLIFFLFLLLPLFIVVSQGLKNGLGAFFTA
CCCHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFEPDALSALKLTVIAVLVSVPLNLVFGVSAAWCVSKYSFRGKSMLVTLIDLPFSVSPVI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
AGLVYVLMFGAQGLFGPWLQDHDIQIVFALPGIVLATIFVTVPFVARELIPLMQEQGTQE
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EEAARLLGANGWQMFWHVTVPNIKWGLIYGVVLCTARAMGEFGAVSVVSGHIRGVTNTLP
HHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
LHVEILYNEYNHVAAFAVASLLLILALFILLLKQWSENRINRLRASAAEE
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]