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The map label for this gene is cysU [H]

Identifier: 77456423

GI number: 77456423

Start: 235577

End: 236395

Strand: Direct

Name: cysU [H]

Synonym: Pfl01_0195

Alternate gene names: 77456423

Gene position: 235577-236395 (Clockwise)

Preceding gene: 77456422

Following gene: 77456424

Centisome position: 3.66

GC content: 63.74

Gene sequence:

>819_bases
ATGTCGCGTCGTATCTCCCCCGTCATACCCGGCTTCGGGCTGACGCTGGGCTACACCTTGGTGTACCTCAGCCTGATTGT
GCTTATCCCGCTGGCGGCGATGTTCGTCCACGCCGCCCAACTCACCTGGGATCAGTTCTGGACGATCATCTCCGCGCCCC
GCGTCCTCGCCGCACTGAAGCTGAGCTTCGGCACCGCGCTGTGCGCCGCGATCATCAACGGCATCATCGGCACGCTGCTG
GCGTGGGTGCTGGTGCGTTACACGTTCCCGGGGCGCAAGGTGATCGACGCGATGATCGATCTGCCGTTCGCCCTGCCGAC
CGCGGTGGCCGGTATTGCCCTGACGGCGCTGTACACGCCGACCGGGCTGGTCGGGCAGTTCGCCGCTGACCTCGGTTTCA
AGATCGCCTACACCCCGCTCGGGATCACGTTGGCGCTGACCTTCGTGACCCTGCCGTTCGTGGTGCGCACGGTACAGCCG
GTGCTGGCCGACATCCCGCGTGAAGTCGAAGAAGCGGCCGCGTGCCTGGGTGCCAAACCGTTGCAGGTGTTCCGCCACAT
CCTGCTGCCGGCGCTGTTGCCGGCCTGGTTGACCGGTTTTGCCCTGGCGTTTGCCCGGGGCGTCGGCGAGTACGGTTCGG
TGATTTTCATCGCCGGCAACATGCCGATGAAAACCGAGATCCTGCCGCTGCTGATCATGGTCAAGCTCGACCAGTACGAT
TACACCGGCGCTACCTCCATCGGCGTATTGATGCTGGTGGTTTCCTTCGTCCTGTTGCTGCTGATCAACTTGCTGCAGCG
GCGCATCGAAACCCCATAA

Upstream 100 bases:

>100_bases
GTGTTCGACCAGATCTATCAGGCGCAGTAAGTTTCAATAGCCTAAAACGAGCCCCGGATTTATGTTCGGGGCTTTGTGCG
TTCTCAAACCAAGGACTTTT

Downstream 100 bases:

>100_bases
GGAGGCGCGAACCATGTCCCAATCGTCTATTGCGGCCGCGTCCTCGGCCAACGCCGCCCGCCGTGGCAGCGCCACTTCGC
GGCGGATCCTGATCGGCCTC

Product: Sulfate ABC transporter, permease CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSRRISPVIPGFGLTLGYTLVYLSLIVLIPLAAMFVHAAQLTWDQFWTIISAPRVLAALKLSFGTALCAAIINGIIGTLL
AWVLVRYTFPGRKVIDAMIDLPFALPTAVAGIALTALYTPTGLVGQFAADLGFKIAYTPLGITLALTFVTLPFVVRTVQP
VLADIPREVEEAAACLGAKPLQVFRHILLPALLPAWLTGFALAFARGVGEYGSVIFIAGNMPMKTEILPLLIMVKLDQYD
YTGATSIGVLMLVVSFVLLLLINLLQRRIETP

Sequences:

>Translated_272_residues
MSRRISPVIPGFGLTLGYTLVYLSLIVLIPLAAMFVHAAQLTWDQFWTIISAPRVLAALKLSFGTALCAAIINGIIGTLL
AWVLVRYTFPGRKVIDAMIDLPFALPTAVAGIALTALYTPTGLVGQFAADLGFKIAYTPLGITLALTFVTLPFVVRTVQP
VLADIPREVEEAAACLGAKPLQVFRHILLPALLPAWLTGFALAFARGVGEYGSVIFIAGNMPMKTEILPLLIMVKLDQYD
YTGATSIGVLMLVVSFVLLLLINLLQRRIETP
>Mature_271_residues
SRRISPVIPGFGLTLGYTLVYLSLIVLIPLAAMFVHAAQLTWDQFWTIISAPRVLAALKLSFGTALCAAIINGIIGTLLA
WVLVRYTFPGRKVIDAMIDLPFALPTAVAGIALTALYTPTGLVGQFAADLGFKIAYTPLGITLALTFVTLPFVVRTVQPV
LADIPREVEEAAACLGAKPLQVFRHILLPALLPAWLTGFALAFARGVGEYGSVIFIAGNMPMKTEILPLLIMVKLDQYDY
TGATSIGVLMLVVSFVLLLLINLLQRRIETP

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=267, Percent_Identity=49.438202247191, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI87082099, Length=265, Percent_Identity=32.4528301886792, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1786980, Length=194, Percent_Identity=34.020618556701, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1786254, Length=272, Percent_Identity=26.4705882352941, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787368, Length=236, Percent_Identity=30.0847457627119, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1787081, Length=227, Percent_Identity=32.5991189427313, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29337; Mature: 29206

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRISPVIPGFGLTLGYTLVYLSLIVLIPLAAMFVHAAQLTWDQFWTIISAPRVLAALK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSFGTALCAAIINGIIGTLLAWVLVRYTFPGRKVIDAMIDLPFALPTAVAGIALTALYTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
TGLVGQFAADLGFKIAYTPLGITLALTFVTLPFVVRTVQPVLADIPREVEEAAACLGAKP
CHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LQVFRHILLPALLPAWLTGFALAFARGVGEYGSVIFIAGNMPMKTEILPLLIMVKLDQYD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCC
YTGATSIGVLMLVVSFVLLLLINLLQRRIETP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRRISPVIPGFGLTLGYTLVYLSLIVLIPLAAMFVHAAQLTWDQFWTIISAPRVLAALK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSFGTALCAAIINGIIGTLLAWVLVRYTFPGRKVIDAMIDLPFALPTAVAGIALTALYTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
TGLVGQFAADLGFKIAYTPLGITLALTFVTLPFVVRTVQPVLADIPREVEEAAACLGAKP
CHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LQVFRHILLPALLPAWLTGFALAFARGVGEYGSVIFIAGNMPMKTEILPLLIMVKLDQYD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCC
YTGATSIGVLMLVVSFVLLLLINLLQRRIETP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]