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The map label for this gene is pntB [H]

Identifier: 77456340

GI number: 77456340

Start: 114025

End: 115458

Strand: Reverse

Name: pntB [H]

Synonym: Pfl01_0112

Alternate gene names: 77456340

Gene position: 115458-114025 (Counterclockwise)

Preceding gene: 77456341

Following gene: 77456339

Centisome position: 1.79

GC content: 60.74

Gene sequence:

>1434_bases
ATGAGCATGAACCTCGTCACGACGCTCTACCTGATCGCATCGATCTGCTTCATCCAGGCCCTCAAAGGCCTGTCGCACCC
GACCACGTCGCGCCGCGGCAACCTGTTCGGCATGCTCGGCATGGCGCTGGCAATCATCACCACGATCGGCCTCATCTATA
AGCTGGGCGCTGAGCTGGCGACCGCAGGCATCGGTTATGTAATCGTCGGCCTGCTGATCGGCGGCACCGCCGGTTCGATC
ATGGCCAAGCGCGTTGAAATGACCAAGATGCCGGAACTGGTTGCGTTCATGCACAGCATGATCGGCCTGGCAGCGGTGTT
CATCGCCATTGCGGCCGTGGTCGAGCCGCAGTCGCTGGGCATCGTCAAGCAACTGGGCGACTCGATCCCGGCGGGTAACC
GTCTGGAGCTGTTCCTCGGCGCAGCCATCGGTGCAATCACCTTCTCCGGTTCGGTGATCGCTTTCGGCAAGCTGTCGGGC
AAGTACAAGTTCCGTCTGTTCCAGGGTGCACCGGTACAGTTCAGCGGTCAGCACAAGCTGAACGCAGTACTGGGTCTGGC
GACGCTGATCCTCGGCGTCACTTTCATGCTGACCGGCAACCTCGCCGCGTTCGCACTGATGCTGGCCCTGGCGTTCGTCA
TGGGCGTGCTGATCATCATCCCGATCGGCGGCGCCGACATGCCAGTGGTGGTGTCGATGCTCAACAGCTATTCCGGCTGG
GCGGCGGCGGGTATCGGCTTCTCGCTGAACAACTCGATGCTGATCATTGCAGGTTCGCTGGTGGGTTCGAGCGGTGCGAT
CCTCTCGTACATCATGTGCAAGGCGATGAACCGTTCCTTCTTTAATGTACTGCTCGGCGGTTTCGGCAATACAGCAGACG
CCGGTCCTGCCGGCGAGAAAGAAGCCCGTCCGGTGAAATCCGGTTCGGCTGACGACGCGACTTTCCTGCTGACCAACGCT
GACACCGTGATCATCGTTCCGGGTTACGGCCTGGCGGTGGCTCGCGCGCAGCATGCCCTGAAAGAGCTGACCGAGAAGCT
GACCCACCACGGCGTGACCGTGAAGTACGCGATCCACCCGGTGGCCGGTCGGATGCCTGGCCACATGAACGTACTGTTGG
CCGAGGCCGAAGTGCCGTACGACCAGGTGTTCGAAATGGAAGACATCAACTCCGAGTTCGGCCAGGCCGACGTGGTGCTG
GTGCTCGGCGCCAACGACGTGGTCAACCCGGCCGCCAAGAACGATCCGAAATCGCCGATTGCCGGCATGCCGATCCTCGA
AGCGTTCAAGGCCAAGACCATCATCGTCAACAAGCGCTCGATGGCCAGCGGCTATGCCGGCCTGGACAACGAACTGTTCT
ATCTGGACAAGACCATGATGGTGTTCGGCGACGCGAAAAAAGTCATCGAAGACATGGTCAAAGCGGTCGAGTAA

Upstream 100 bases:

>100_bases
TGGCCGCGGTCAATGTGTTCGGTGGCTTCCTGGTCACCCGCCGCATGCTTGAGATGTTCAAGAAGAAAGCCCCGAAAGCA
AAAGAAGAGGCGCCTAAGTA

Downstream 100 bases:

>100_bases
CCCTCGCCGGCAACACCGAACGCCCCGACTTGTCGGGGCGTTTTTGTTTGCGCAAACCGTCGGACAATTTGCCGCGTTGT
TGCAGATCCAGTAACGGTGT

Product: NAD(P) transhydrogenase subunit beta

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSMNLVTTLYLIASICFIQALKGLSHPTTSRRGNLFGMLGMALAIITTIGLIYKLGAELATAGIGYVIVGLLIGGTAGSI
MAKRVEMTKMPELVAFMHSMIGLAAVFIAIAAVVEPQSLGIVKQLGDSIPAGNRLELFLGAAIGAITFSGSVIAFGKLSG
KYKFRLFQGAPVQFSGQHKLNAVLGLATLILGVTFMLTGNLAAFALMLALAFVMGVLIIIPIGGADMPVVVSMLNSYSGW
AAAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVLLGGFGNTADAGPAGEKEARPVKSGSADDATFLLTNA
DTVIIVPGYGLAVARAQHALKELTEKLTHHGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDINSEFGQADVVL
VLGANDVVNPAAKNDPKSPIAGMPILEAFKAKTIIVNKRSMASGYAGLDNELFYLDKTMMVFGDAKKVIEDMVKAVE

Sequences:

>Translated_477_residues
MSMNLVTTLYLIASICFIQALKGLSHPTTSRRGNLFGMLGMALAIITTIGLIYKLGAELATAGIGYVIVGLLIGGTAGSI
MAKRVEMTKMPELVAFMHSMIGLAAVFIAIAAVVEPQSLGIVKQLGDSIPAGNRLELFLGAAIGAITFSGSVIAFGKLSG
KYKFRLFQGAPVQFSGQHKLNAVLGLATLILGVTFMLTGNLAAFALMLALAFVMGVLIIIPIGGADMPVVVSMLNSYSGW
AAAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVLLGGFGNTADAGPAGEKEARPVKSGSADDATFLLTNA
DTVIIVPGYGLAVARAQHALKELTEKLTHHGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDINSEFGQADVVL
VLGANDVVNPAAKNDPKSPIAGMPILEAFKAKTIIVNKRSMASGYAGLDNELFYLDKTMMVFGDAKKVIEDMVKAVE
>Mature_476_residues
SMNLVTTLYLIASICFIQALKGLSHPTTSRRGNLFGMLGMALAIITTIGLIYKLGAELATAGIGYVIVGLLIGGTAGSIM
AKRVEMTKMPELVAFMHSMIGLAAVFIAIAAVVEPQSLGIVKQLGDSIPAGNRLELFLGAAIGAITFSGSVIAFGKLSGK
YKFRLFQGAPVQFSGQHKLNAVLGLATLILGVTFMLTGNLAAFALMLALAFVMGVLIIIPIGGADMPVVVSMLNSYSGWA
AAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVLLGGFGNTADAGPAGEKEARPVKSGSADDATFLLTNAD
TVIIVPGYGLAVARAQHALKELTEKLTHHGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDINSEFGQADVVLV
LGANDVVNPAAKNDPKSPIAGMPILEAFKAKTIIVNKRSMASGYAGLDNELFYLDKTMMVFGDAKKVIEDMVKAVE

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=485, Percent_Identity=46.1855670103093, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI122939153, Length=485, Percent_Identity=46.1855670103093, Blast_Score=393, Evalue=1e-109,
Organism=Escherichia coli, GI1787886, Length=480, Percent_Identity=51.0416666666667, Blast_Score=461, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17550456, Length=477, Percent_Identity=44.2348008385744, Blast_Score=358, Evalue=5e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 49970; Mature: 49839

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMNLVTTLYLIASICFIQALKGLSHPTTSRRGNLFGMLGMALAIITTIGLIYKLGAELA
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TAGIGYVIVGLLIGGTAGSIMAKRVEMTKMPELVAFMHSMIGLAAVFIAIAAVVEPQSLG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
IVKQLGDSIPAGNRLELFLGAAIGAITFSGSVIAFGKLSGKYKFRLFQGAPVQFSGQHKL
HHHHHCCCCCCCCEEEEEHHHHHHHHEECCCEEEEEECCCEEEEEEEECCCCEECCCHHH
NAVLGLATLILGVTFMLTGNLAAFALMLALAFVMGVLIIIPIGGADMPVVVSMLNSYSGW
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHCCCCC
AAAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVLLGGFGNTADAGPAGEK
EEECCCEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
EARPVKSGSADDATFLLTNADTVIIVPGYGLAVARAQHALKELTEKLTHHGVTVKYAIHP
CCCCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
VAGRMPGHMNVLLAEAEVPYDQVFEMEDINSEFGQADVVLVLGANDVVNPAAKNDPKSPI
CCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
AGMPILEAFKAKTIIVNKRSMASGYAGLDNELFYLDKTMMVFGDAKKVIEDMVKAVE
CCCHHHHHHCCCEEEEECHHHHCCCCCCCCCEEEECCHHHEECCHHHHHHHHHHHCC
>Mature Secondary Structure 
SMNLVTTLYLIASICFIQALKGLSHPTTSRRGNLFGMLGMALAIITTIGLIYKLGAELA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TAGIGYVIVGLLIGGTAGSIMAKRVEMTKMPELVAFMHSMIGLAAVFIAIAAVVEPQSLG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
IVKQLGDSIPAGNRLELFLGAAIGAITFSGSVIAFGKLSGKYKFRLFQGAPVQFSGQHKL
HHHHHCCCCCCCCEEEEEHHHHHHHHEECCCEEEEEECCCEEEEEEEECCCCEECCCHHH
NAVLGLATLILGVTFMLTGNLAAFALMLALAFVMGVLIIIPIGGADMPVVVSMLNSYSGW
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHCCCCC
AAAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVLLGGFGNTADAGPAGEK
EEECCCEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
EARPVKSGSADDATFLLTNADTVIIVPGYGLAVARAQHALKELTEKLTHHGVTVKYAIHP
CCCCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
VAGRMPGHMNVLLAEAEVPYDQVFEMEDINSEFGQADVVLVLGANDVVNPAAKNDPKSPI
CCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
AGMPILEAFKAKTIIVNKRSMASGYAGLDNELFYLDKTMMVFGDAKKVIEDMVKAVE
CCCHHHHHHCCCEEEEECHHHHCCCCCCCCCEEEECCHHHEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]