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The map label for this gene is pleD [H]

Identifier: 77456278

GI number: 77456278

Start: 55155

End: 57245

Strand: Reverse

Name: pleD [H]

Synonym: Pfl01_0050

Alternate gene names: 77456278

Gene position: 57245-55155 (Counterclockwise)

Preceding gene: 77456279

Following gene: 77456277

Centisome position: 0.89

GC content: 62.98

Gene sequence:

>2091_bases
ATGAGCGACGAAGCCCAGCGCTGGAAAGAGAAATACCTCAAAAGCATCGAACAACAGGAAAAGCTCGAACGTCGATGGGA
CGCCCGGCTCGACCTGCTGCGCCGCGGCCTGGTGCGCAGCACCCTGGCGGCGGAAGGCACCGACCGCGTGGTCGATCAGT
GCATGAAAGAGATGCGCGACGTGGTGCGCACCGATGACATGGACGCCGGCCTCGCCGCCCTGCTGCCACGCCTGGAAAAA
GCCGTGCTCGATTCCGAGCAGCGCCGGGAAACCCGGATCGAGCAGGTCAGCACCGCACTGACTGCACTGGTCACTCAATT
GCAATCCCTGCCGCTGCCCCGAGAAGTGGCCCAGCCGCTGAAGAAGTTCGCCAAGCAGCTGGACGGCCGGGTGAGTCAGG
CAAGGGAAATGCCGCTGCTGCTCAGCGAACTCAGCGGCCTGCAGGGCAAGGCCTTGAGTCAGCTGGCGACGCCGACGGAA
CCGGGGCGTCCCGGGTTGTTGCAGCGCCTGTTCGGCAGCCGCGACAGCGAGGAAACCCCTGCTCAAGTGGCCCAGGCTCC
TGCGGTGACTCCGTCAGTGCAGCCAGAGCAACCACCCGTCACACATGAAGCTGTGCCAGCGCCGCAAGCGCCTGTTACAA
GCGCTGAACAGGTCGCCACTGCGCCCGTCAGCCCAGCGCTGATCGCCGCCCCCGTGGCCGAACCATCCAAAGCTCCAGAG
CCAATCCCAGAAGACTTTACAGAGCCGGAGGTGGTCGCATTTGAGCCGGCCAGAATCCTCACCGAAGCGCCCAGCGCTCC
CAGCGAACCAGAACCCGTCGAGCCGGAATCAAAGCTGCAACCCTTCACGCCTCCTCCGGTTTTCGCGCCGGCAGTGCCCA
CGGCCATCAACCCCGACGAGTTGATCCTCCCCGGCGATACCCCTCCGCCCGTGATCCTCGACAGCCTGCCCCTGCCCGAA
CCCATCGCCCAGGCATTGGCCGCCATCGATCCGGAACAATCCGAGCATGACATTCTCTATGCCCTGCCGGACTCGCCAGA
GCCGTCCTACAGCTCGGTCGCCCGACACATCGAAGACACGCTGATTGGCTTGCTGGATGACCTGACACTGCCGGAGCGCC
ACCGCCCGCAAGCCGAAGCCATGCGCGACCGGCTGAAAAATGGCCTTAACTGGTACGAACTACTACCGATCCTCGACGAT
CTTGCGACATTGATGCTGGCCATCACAGACAGCGGCCAGCACGAATTCGAAGCTTATCTGCAGCAACTCAATGAACGCCT
CGAGGCATTCCAGAGCAACTTGCAGGCTGCCAGCGAAGGACACGCCGACAACAGCTCCGCCGCGAGAGCAATGGACACGC
AGATCCGCGAACAGGTCGACGGCTTGCAGACCAGCGTCCAGGAGGCGGCGGATCTGAATGATCTCAAGCAAGTGCTGGAA
AACCACCTCGAGGGCCTGCTCGGCACCATGGATCAGCACCAGAAGCAGCGCGACGCTCGGGAACAGGAAGTCGCCGGACG
CCTGAAAAGCCTGGCCGAGCGCGTCGCGCACATGGAGCAGGAAGCGCTGGGCTATCGCGAACACCTCGAAGAGCAGCGCC
AGAAAGCCCTGATCGATCCGCTCACCGGCCTGCCCAACCGCGCGGCCTGGACCGAACGGCTCGAACATGAAATCGCCCAG
TGGCAGCAGCACGGCAACACTCTGAGCCTGGCTATGCTCGACCTCGATCACTTCAAGCGGATCAACGACAACTACGGGCA
CCTGGCCGGTGACAAGGTGTTGAAGATCATCGCCACAGTCCTGCGCAAGCGTATGCGTGGCAACGATTTCATCGCTCGTT
TCGGCGGTGAGGAGTTTGTCCTGCTGCTTCCAGCCACCCCACCAGCGGTCGGCGCCAAACTCCTGGAAAACCTACGCGCC
TCCATCGAGGCCTGCCCGTTTCACTTCAAGGGCGAGCGCGTCACGATCACGATCTCAATGGGACTGGCGGCGTTTCGGCC
GGGAGAACACAGCGATCTGGTGCTCAAAAGAGCCGATCAGGCGCTTTATCGGGCGAAAAACGCGGGACGTAACCGGGTGG
AGCTGGGCTGA

Upstream 100 bases:

>100_bases
TTTCCGATCACCTGCCGGTCGCGGTAGAGATTCGTCTGCCGGGTTCGCTCACGGCCGATGCATTGCCCGCGTTGAGCACT
GCCCTTCGCGGAACCCCTGA

Downstream 100 bases:

>100_bases
GGCAATTGTTCCATATTGTTTAAATCAGCCCTTTAAGCGTCGAAGCGCAAGGCGATACGTTACACTGTTGCATTATCGTC
TTGCGTGTACTGCCCTCTGC

Product: putative diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 696; Mature: 695

Protein sequence:

>696_residues
MSDEAQRWKEKYLKSIEQQEKLERRWDARLDLLRRGLVRSTLAAEGTDRVVDQCMKEMRDVVRTDDMDAGLAALLPRLEK
AVLDSEQRRETRIEQVSTALTALVTQLQSLPLPREVAQPLKKFAKQLDGRVSQAREMPLLLSELSGLQGKALSQLATPTE
PGRPGLLQRLFGSRDSEETPAQVAQAPAVTPSVQPEQPPVTHEAVPAPQAPVTSAEQVATAPVSPALIAAPVAEPSKAPE
PIPEDFTEPEVVAFEPARILTEAPSAPSEPEPVEPESKLQPFTPPPVFAPAVPTAINPDELILPGDTPPPVILDSLPLPE
PIAQALAAIDPEQSEHDILYALPDSPEPSYSSVARHIEDTLIGLLDDLTLPERHRPQAEAMRDRLKNGLNWYELLPILDD
LATLMLAITDSGQHEFEAYLQQLNERLEAFQSNLQAASEGHADNSSAARAMDTQIREQVDGLQTSVQEAADLNDLKQVLE
NHLEGLLGTMDQHQKQRDAREQEVAGRLKSLAERVAHMEQEALGYREHLEEQRQKALIDPLTGLPNRAAWTERLEHEIAQ
WQQHGNTLSLAMLDLDHFKRINDNYGHLAGDKVLKIIATVLRKRMRGNDFIARFGGEEFVLLLPATPPAVGAKLLENLRA
SIEACPFHFKGERVTITISMGLAAFRPGEHSDLVLKRADQALYRAKNAGRNRVELG

Sequences:

>Translated_696_residues
MSDEAQRWKEKYLKSIEQQEKLERRWDARLDLLRRGLVRSTLAAEGTDRVVDQCMKEMRDVVRTDDMDAGLAALLPRLEK
AVLDSEQRRETRIEQVSTALTALVTQLQSLPLPREVAQPLKKFAKQLDGRVSQAREMPLLLSELSGLQGKALSQLATPTE
PGRPGLLQRLFGSRDSEETPAQVAQAPAVTPSVQPEQPPVTHEAVPAPQAPVTSAEQVATAPVSPALIAAPVAEPSKAPE
PIPEDFTEPEVVAFEPARILTEAPSAPSEPEPVEPESKLQPFTPPPVFAPAVPTAINPDELILPGDTPPPVILDSLPLPE
PIAQALAAIDPEQSEHDILYALPDSPEPSYSSVARHIEDTLIGLLDDLTLPERHRPQAEAMRDRLKNGLNWYELLPILDD
LATLMLAITDSGQHEFEAYLQQLNERLEAFQSNLQAASEGHADNSSAARAMDTQIREQVDGLQTSVQEAADLNDLKQVLE
NHLEGLLGTMDQHQKQRDAREQEVAGRLKSLAERVAHMEQEALGYREHLEEQRQKALIDPLTGLPNRAAWTERLEHEIAQ
WQQHGNTLSLAMLDLDHFKRINDNYGHLAGDKVLKIIATVLRKRMRGNDFIARFGGEEFVLLLPATPPAVGAKLLENLRA
SIEACPFHFKGERVTITISMGLAAFRPGEHSDLVLKRADQALYRAKNAGRNRVELG
>Mature_695_residues
SDEAQRWKEKYLKSIEQQEKLERRWDARLDLLRRGLVRSTLAAEGTDRVVDQCMKEMRDVVRTDDMDAGLAALLPRLEKA
VLDSEQRRETRIEQVSTALTALVTQLQSLPLPREVAQPLKKFAKQLDGRVSQAREMPLLLSELSGLQGKALSQLATPTEP
GRPGLLQRLFGSRDSEETPAQVAQAPAVTPSVQPEQPPVTHEAVPAPQAPVTSAEQVATAPVSPALIAAPVAEPSKAPEP
IPEDFTEPEVVAFEPARILTEAPSAPSEPEPVEPESKLQPFTPPPVFAPAVPTAINPDELILPGDTPPPVILDSLPLPEP
IAQALAAIDPEQSEHDILYALPDSPEPSYSSVARHIEDTLIGLLDDLTLPERHRPQAEAMRDRLKNGLNWYELLPILDDL
ATLMLAITDSGQHEFEAYLQQLNERLEAFQSNLQAASEGHADNSSAARAMDTQIREQVDGLQTSVQEAADLNDLKQVLEN
HLEGLLGTMDQHQKQRDAREQEVAGRLKSLAERVAHMEQEALGYREHLEEQRQKALIDPLTGLPNRAAWTERLEHEIAQW
QQHGNTLSLAMLDLDHFKRINDNYGHLAGDKVLKIIATVLRKRMRGNDFIARFGGEEFVLLLPATPPAVGAKLLENLRAS
IEACPFHFKGERVTITISMGLAAFRPGEHSDLVLKRADQALYRAKNAGRNRVELG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=42.4418604651163, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786584, Length=184, Percent_Identity=37.5, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI145693134, Length=219, Percent_Identity=31.9634703196347, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787802, Length=169, Percent_Identity=35.5029585798817, Blast_Score=107, Evalue=4e-24,
Organism=Escherichia coli, GI1787816, Length=163, Percent_Identity=36.8098159509202, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI1787262, Length=246, Percent_Identity=28.8617886178862, Blast_Score=93, Evalue=7e-20,
Organism=Escherichia coli, GI87082007, Length=163, Percent_Identity=37.4233128834356, Blast_Score=93, Evalue=7e-20,
Organism=Escherichia coli, GI1787541, Length=167, Percent_Identity=34.7305389221557, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788381, Length=169, Percent_Identity=31.9526627218935, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=29.2207792207792, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788085, Length=200, Percent_Identity=29.5, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1788956, Length=190, Percent_Identity=30.5263157894737, Blast_Score=70, Evalue=6e-13,
Organism=Escherichia coli, GI87081977, Length=209, Percent_Identity=29.1866028708134, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787056, Length=168, Percent_Identity=30.3571428571429, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 76952; Mature: 76821

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDEAQRWKEKYLKSIEQQEKLERRWDARLDLLRRGLVRSTLAAEGTDRVVDQCMKEMRD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VVRTDDMDAGLAALLPRLEKAVLDSEQRRETRIEQVSTALTALVTQLQSLPLPREVAQPL
HHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
KKFAKQLDGRVSQAREMPLLLSELSGLQGKALSQLATPTEPGRPGLLQRLFGSRDSEETP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCH
AQVAQAPAVTPSVQPEQPPVTHEAVPAPQAPVTSAEQVATAPVSPALIAAPVAEPSKAPE
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHCCCCCCCCCCCC
PIPEDFTEPEVVAFEPARILTEAPSAPSEPEPVEPESKLQPFTPPPVFAPAVPTAINPDE
CCCCCCCCCCEEEECHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCC
LILPGDTPPPVILDSLPLPEPIAQALAAIDPEQSEHDILYALPDSPEPSYSSVARHIEDT
EEECCCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
LIGLLDDLTLPERHRPQAEAMRDRLKNGLNWYELLPILDDLATLMLAITDSGQHEFEAYL
HHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QQLNERLEAFQSNLQAASEGHADNSSAARAMDTQIREQVDGLQTSVQEAADLNDLKQVLE
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
NHLEGLLGTMDQHQKQRDAREQEVAGRLKSLAERVAHMEQEALGYREHLEEQRQKALIDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTGLPNRAAWTERLEHEIAQWQQHGNTLSLAMLDLDHFKRINDNYGHLAGDKVLKIIATV
HCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LRKRMRGNDFIARFGGEEFVLLLPATPPAVGAKLLENLRASIEACPFHFKGERVTITISM
HHHHHCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEECCCEEEEEEEC
GLAAFRPGEHSDLVLKRADQALYRAKNAGRNRVELG
CHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SDEAQRWKEKYLKSIEQQEKLERRWDARLDLLRRGLVRSTLAAEGTDRVVDQCMKEMRD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VVRTDDMDAGLAALLPRLEKAVLDSEQRRETRIEQVSTALTALVTQLQSLPLPREVAQPL
HHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
KKFAKQLDGRVSQAREMPLLLSELSGLQGKALSQLATPTEPGRPGLLQRLFGSRDSEETP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCH
AQVAQAPAVTPSVQPEQPPVTHEAVPAPQAPVTSAEQVATAPVSPALIAAPVAEPSKAPE
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHCCCCCCCCCCCC
PIPEDFTEPEVVAFEPARILTEAPSAPSEPEPVEPESKLQPFTPPPVFAPAVPTAINPDE
CCCCCCCCCCEEEECHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCC
LILPGDTPPPVILDSLPLPEPIAQALAAIDPEQSEHDILYALPDSPEPSYSSVARHIEDT
EEECCCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
LIGLLDDLTLPERHRPQAEAMRDRLKNGLNWYELLPILDDLATLMLAITDSGQHEFEAYL
HHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QQLNERLEAFQSNLQAASEGHADNSSAARAMDTQIREQVDGLQTSVQEAADLNDLKQVLE
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
NHLEGLLGTMDQHQKQRDAREQEVAGRLKSLAERVAHMEQEALGYREHLEEQRQKALIDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTGLPNRAAWTERLEHEIAQWQQHGNTLSLAMLDLDHFKRINDNYGHLAGDKVLKIIATV
HCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LRKRMRGNDFIARFGGEEFVLLLPATPPAVGAKLLENLRASIEACPFHFKGERVTITISM
HHHHHCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEECCCEEEEEEEC
GLAAFRPGEHSDLVLKRADQALYRAKNAGRNRVELG
CHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA