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The map label for this gene is llpE [H]

Identifier: 76818851

GI number: 76818851

Start: 2228640

End: 2229590

Strand: Direct

Name: llpE [H]

Synonym: BURPS1710b_A1839

Alternate gene names: 76818851

Gene position: 2228640-2229590 (Clockwise)

Preceding gene: 76818290

Following gene: 76818881

Centisome position: 70.04

GC content: 69.93

Gene sequence:

>951_bases
TTGTGTTGCCGGATTATAGGCATTTATCCCGATGTCTGCCGGACCCAGAATGCGATCCATCTCGCAATGGACGCGCGACG
CCGCGCAACACACGTCATGGACGCATTCGATTTCCGCCGCCAAATTTCCAGCACGAGCGCCGAAAGCGCGCGCGTGCGGC
CGGCGCTCGCGGTGTCGGACGTCGTGATCGGCGGCTACGCGCAGGACATTGCGCTGCGTCTGTATCGGCGCCCCGATAAA
TCCTCGCTGCCGGTGGTGCTTTATTTCCACGGCGGCGGCTTCGTGCGCGGTTCGCTCGACGACGCCGAGCGCGCCGCGCG
CCATCTCGCCGAGCACGTGCCGGCGCTCGTCGTGTCGGTCGATTATTCGCTCGCGCCGCGGCACCCGTTTCCGGCCGCGC
CGGAAGATGCGTACTGCGCGGCGCGCTGGGCCGAAGCCCGCGCGCGCGCGTTCGGCGGCAATCCGCGCAAGATCGGCGTG
GCCGGCCACGACGCGGGCGGCCAGCTCGCGAACTGCCTGGCGTTCATCGCGCGCGATCGCGGCGAGATTCGCATCGCCGC
GCAGGCGCTGATCGGCCCGATGCTCGATCCGAGCCTCACGCGGCTCGCCGACGCGACGCGTCTCGCGTCGTCCGACACGA
GCGCGCAGGAGTGCGCCGCGTGCTACCGCGCATACCTGCCGCAGCCGTCGCAGCGGATGCATCCGTACGCGGCGCCGCTC
GAATCGGTGCGCCTCGCGGGGCTGCCGCCGACGCTCGTCGCGACCGCGCAGAACGACGTGCTGCACGTCGAGGCGGAAAA
GTACGCGAGCTGTCTGATCGACGCGGGCGTGCCGACGCAGGTGGTGCGCTATCGGGACGTGTCGCATGCGGCGCTCGCCG
ATCACGCGAGCGCGCTCGAAGAGGTGGCGCGTTTCTTCCAGTTCCGCTTCAGCGCGGGTGCACCGGGCTGA

Upstream 100 bases:

>100_bases
CGGTCCATTACTCTACCTCCGCGATATTGGCACCCCGAACGGATGCAGGCCGCGACACCGGTGAAGGCGACGCAGCCGAC
GATTGTTCAGGGGACATGAA

Downstream 100 bases:

>100_bases
CGCCGTTTTCATCCGAATTCAGAACAACCGACAACCTGGGGGTTTTTTCATGGCTATCTTGCGTACTTCCCGTTCGCGGA
TCGCGGCCGCGGCGTTCGCG

Product: lipase-like protein LlpE

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MCCRIIGIYPDVCRTQNAIHLAMDARRRATHVMDAFDFRRQISSTSAESARVRPALAVSDVVIGGYAQDIALRLYRRPDK
SSLPVVLYFHGGGFVRGSLDDAERAARHLAEHVPALVVSVDYSLAPRHPFPAAPEDAYCAARWAEARARAFGGNPRKIGV
AGHDAGGQLANCLAFIARDRGEIRIAAQALIGPMLDPSLTRLADATRLASSDTSAQECAACYRAYLPQPSQRMHPYAAPL
ESVRLAGLPPTLVATAQNDVLHVEAEKYASCLIDAGVPTQVVRYRDVSHAALADHASALEEVARFFQFRFSAGAPG

Sequences:

>Translated_316_residues
MCCRIIGIYPDVCRTQNAIHLAMDARRRATHVMDAFDFRRQISSTSAESARVRPALAVSDVVIGGYAQDIALRLYRRPDK
SSLPVVLYFHGGGFVRGSLDDAERAARHLAEHVPALVVSVDYSLAPRHPFPAAPEDAYCAARWAEARARAFGGNPRKIGV
AGHDAGGQLANCLAFIARDRGEIRIAAQALIGPMLDPSLTRLADATRLASSDTSAQECAACYRAYLPQPSQRMHPYAAPL
ESVRLAGLPPTLVATAQNDVLHVEAEKYASCLIDAGVPTQVVRYRDVSHAALADHASALEEVARFFQFRFSAGAPG
>Mature_316_residues
MCCRIIGIYPDVCRTQNAIHLAMDARRRATHVMDAFDFRRQISSTSAESARVRPALAVSDVVIGGYAQDIALRLYRRPDK
SSLPVVLYFHGGGFVRGSLDDAERAARHLAEHVPALVVSVDYSLAPRHPFPAAPEDAYCAARWAEARARAFGGNPRKIGV
AGHDAGGQLANCLAFIARDRGEIRIAAQALIGPMLDPSLTRLADATRLASSDTSAQECAACYRAYLPQPSQRMHPYAAPL
ESVRLAGLPPTLVATAQNDVLHVEAEKYASCLIDAGVPTQVVRYRDVSHAALADHASALEEVARFFQFRFSAGAPG

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=291, Percent_Identity=28.1786941580756, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI68299767, Length=331, Percent_Identity=25.6797583081571, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI21328446, Length=109, Percent_Identity=36.697247706422, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786682, Length=242, Percent_Identity=30.5785123966942, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI72001146, Length=147, Percent_Identity=31.9727891156463, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34130; Mature: 34130

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCCRIIGIYPDVCRTQNAIHLAMDARRRATHVMDAFDFRRQISSTSAESARVRPALAVSD
CCEEEEECCHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHH
VVIGGYAQDIALRLYRRPDKSSLPVVLYFHGGGFVRGSLDDAERAARHLAEHVPALVVSV
HHCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHHHCCEEEEEE
DYSLAPRHPFPAAPEDAYCAARWAEARARAFGGNPRKIGVAGHDAGGQLANCLAFIARDR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCC
GEIRIAAQALIGPMLDPSLTRLADATRLASSDTSAQECAACYRAYLPQPSQRMHPYAAPL
CCEEEEHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHCCCCCCCH
ESVRLAGLPPTLVATAQNDVLHVEAEKYASCLIDAGVPTQVVRYRDVSHAALADHASALE
HHHEECCCCCCEEEECCCCEEEEEHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHH
EVARFFQFRFSAGAPG
HHHHHHHHHHCCCCCC
>Mature Secondary Structure
MCCRIIGIYPDVCRTQNAIHLAMDARRRATHVMDAFDFRRQISSTSAESARVRPALAVSD
CCEEEEECCHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHH
VVIGGYAQDIALRLYRRPDKSSLPVVLYFHGGGFVRGSLDDAERAARHLAEHVPALVVSV
HHCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHHHCCEEEEEE
DYSLAPRHPFPAAPEDAYCAARWAEARARAFGGNPRKIGVAGHDAGGQLANCLAFIARDR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCC
GEIRIAAQALIGPMLDPSLTRLADATRLASSDTSAQECAACYRAYLPQPSQRMHPYAAPL
CCEEEEHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHCCCCCCCH
ESVRLAGLPPTLVATAQNDVLHVEAEKYASCLIDAGVPTQVVRYRDVSHAALADHASALE
HHHEECCCCCCEEEECCCCEEEEEHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHH
EVARFFQFRFSAGAPG
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA