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The map label for this gene is insK [C]

Identifier: 76818452

GI number: 76818452

Start: 234113

End: 234946

Strand: Direct

Name: insK [C]

Synonym: BURPS1710b_A0167

Alternate gene names: 76818452

Gene position: 234113-234946 (Clockwise)

Preceding gene: 76817879

Following gene: 76817549

Centisome position: 7.36

GC content: 61.63

Gene sequence:

>834_bases
GTGTTGGCGATTCGGGAGAAGGTCAACATCTCCGAGCTCCGCGCCTGCCGGCTTGTCGGGCTTTCTCGCAGCGTGCTGCA
TTACGACGCGAAGCCGGACCACGAGAATGAGGTGCTCGCGGCGCGTCTGGTGAAGTTGGCGCACGAACGTCGTCGATTCG
GCTACCGCCGACTGCACGCCCTGGTGGAACGCGAAGGCACGCAGGCCAATCACAAGCGCATCTATCGCCTGTACCGTGAG
GCAGGGCTGGCTGTGCGGCGCCGTCGCAAGCGCCACGGCGTCATGATTGAGCGCGAGCAACTGGCATTGCCGGGCGCACC
CAACGAGGTGTGGTCAATCGATTTCGTGATGGATGCGCTTTCCAACGGCCGGCGCGTGAAGTGCCTGACCGTCGTCGACG
ATTTCACGAAAGAGGCTGTCGACATCGTCGTCGACCATGGCATCTCAGGTTTGTATGTCGCTCGGGCATTGGACCGTGCA
GCTCGCTTCCGTGGCTATCCCAAGGCGGTGCGAACAGACCAGGGACCCGAATTTACGAGCCGCGCGCTTGACCAGTGGGC
GTATGCGAACGGCGTCACGCTGAAGTTGATTCAGGCGGGCAAGCCAACGCAGAATGCGTACATCGAATCGTTCAACGGCA
AGTTCCGCGACGAATGCCTTAACGAGCACTGGTTCACGACGCTCGCGCACGCTCGGGCAGTCATCGCGGCATGGCGTCAG
GACTACAACGAGCAAAGGCCGCACAGCGCACTGAACTACCTTGCGCCGTCAGAGTTTGCGGCGAAACATCGGGCAACCGC
GGACGCTCCTGCCGCTTTCCAGGAGTTGGTTTAA

Upstream 100 bases:

>100_bases
CCCGACTGAAGAAACTGCTGGCCGAAGCAATGCTCGATATGGAAGCGCTGAAGGTTGTCGTCAAGGGAAAGCCCTGAGCC
CGCAAGCCAAACGCGAAGCA

Downstream 100 bases:

>100_bases
AGGGATTTTGCTAGAAGCCCATTGGCCCTATCGAAGGGGGCAGGTCAGCCCTACGTAACGTGGATGCTGAGAATTGCCAC
CGTTGTTACAGGCCGATCCG

Product: IS407A, transposase OrfB

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MLAIREKVNISELRACRLVGLSRSVLHYDAKPDHENEVLAARLVKLAHERRRFGYRRLHALVEREGTQANHKRIYRLYRE
AGLAVRRRRKRHGVMIEREQLALPGAPNEVWSIDFVMDALSNGRRVKCLTVVDDFTKEAVDIVVDHGISGLYVARALDRA
ARFRGYPKAVRTDQGPEFTSRALDQWAYANGVTLKLIQAGKPTQNAYIESFNGKFRDECLNEHWFTTLAHARAVIAAWRQ
DYNEQRPHSALNYLAPSEFAAKHRATADAPAAFQELV

Sequences:

>Translated_277_residues
MLAIREKVNISELRACRLVGLSRSVLHYDAKPDHENEVLAARLVKLAHERRRFGYRRLHALVEREGTQANHKRIYRLYRE
AGLAVRRRRKRHGVMIEREQLALPGAPNEVWSIDFVMDALSNGRRVKCLTVVDDFTKEAVDIVVDHGISGLYVARALDRA
ARFRGYPKAVRTDQGPEFTSRALDQWAYANGVTLKLIQAGKPTQNAYIESFNGKFRDECLNEHWFTTLAHARAVIAAWRQ
DYNEQRPHSALNYLAPSEFAAKHRATADAPAAFQELV
>Mature_277_residues
MLAIREKVNISELRACRLVGLSRSVLHYDAKPDHENEVLAARLVKLAHERRRFGYRRLHALVEREGTQANHKRIYRLYRE
AGLAVRRRRKRHGVMIEREQLALPGAPNEVWSIDFVMDALSNGRRVKCLTVVDDFTKEAVDIVVDHGISGLYVARALDRA
ARFRGYPKAVRTDQGPEFTSRALDQWAYANGVTLKLIQAGKPTQNAYIESFNGKFRDECLNEHWFTTLAHARAVIAAWRQ
DYNEQRPHSALNYLAPSEFAAKHRATADAPAAFQELV

Specific function: Involved in the transposition of the insertion sequence is150 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=262, Percent_Identity=27.4809160305344, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1786462, Length=266, Percent_Identity=23.6842105263158, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31654; Mature: 31654

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAIREKVNISELRACRLVGLSRSVLHYDAKPDHENEVLAARLVKLAHERRRFGYRRLHA
CCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVEREGTQANHKRIYRLYREAGLAVRRRRKRHGVMIEREQLALPGAPNEVWSIDFVMDAL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHCCCCCCCCHHHHHHHHHHHH
SNGRRVKCLTVVDDFTKEAVDIVVDHGISGLYVARALDRAARFRGYPKAVRTDQGPEFTS
CCCCEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHH
RALDQWAYANGVTLKLIQAGKPTQNAYIESFNGKFRDECLNEHWFTTLAHARAVIAAWRQ
HHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYNEQRPHSALNYLAPSEFAAKHRATADAPAAFQELV
HHHHCCCHHHHHHCCCHHHHHHHCCCCCCHHHHHHCC
>Mature Secondary Structure
MLAIREKVNISELRACRLVGLSRSVLHYDAKPDHENEVLAARLVKLAHERRRFGYRRLHA
CCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVEREGTQANHKRIYRLYREAGLAVRRRRKRHGVMIEREQLALPGAPNEVWSIDFVMDAL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHCCCCCCCCHHHHHHHHHHHH
SNGRRVKCLTVVDDFTKEAVDIVVDHGISGLYVARALDRAARFRGYPKAVRTDQGPEFTS
CCCCEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHH
RALDQWAYANGVTLKLIQAGKPTQNAYIESFNGKFRDECLNEHWFTTLAHARAVIAAWRQ
HHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYNEQRPHSALNYLAPSEFAAKHRATADAPAAFQELV
HHHHCCCHHHHHHCCCHHHHHHHCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1718819 [H]