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The map label for this gene is hyfR [H]

Identifier: 76817549

GI number: 76817549

Start: 238891

End: 239532

Strand: Direct

Name: hyfR [H]

Synonym: BURPS1710b_A0169

Alternate gene names: 76817549

Gene position: 238891-239532 (Clockwise)

Preceding gene: 76818452

Following gene: 76817701

Centisome position: 7.51

GC content: 50.93

Gene sequence:

>642_bases
ATGGTCGTCTCGTCTAGTGCAATGCAAGCGCTATTCCGAACAATCAGCAAAGTTGCAAAGAGCGATGCATCTGTATTTAT
TTTCTGCGAATATGGGACGGGGAAAGAGTTGACTGCCACTGAGATCCACAAGCGTTCAGGACGCGCAGACTCTCCATTTG
TCGTGATAAATTGCGATGCGATTCCGCCTCAGTTGTTGCAATCAGAGCTTTTCGGATACGAGCGTGGGGCGTTCACTGGC
GCGACTCATCGAAAAATAGGTAGGCTTGAAGAGGCAGATGGCGGCACACTTTTTCTTGACGAGATTGGTGACATGCAACT
TGAAAGTCAAGTCAATCTTCTGCGCTTCCTTGAAGAGCGAAAACTCCAACGACTTGGCGGCCGCGACTCGATTATGATTG
GCGCTCGCATCATTTCCGCGACGCATTGCGATATAGAGCAGATGATACGAGAAGGGCGCTTTCGCTCCGACCTTTATCAC
CGCCTTTATGTCCTGAGCATCAAGGAGCCGCCGCTTCACGAACGCGATCAGGATATTGAGCTGCTCGCGTGGCACATGCT
GGATCGTTATAAGAATGATCCGGGACGGCGGCTACACGGTCTTTCGGCAGATGCAGTAAGGGCACTCCACAGTTATGATT
AG

Upstream 100 bases:

>100_bases
AGACAGCCATGTAATTTACTCAATCAATCGGGCATTCAGGATGTCGATGACAGGAGAGTTTGATGCAAAGAATGTTAATT
CCTTTGACGACGAATGCGAG

Downstream 100 bases:

>100_bases
CTCGGCAATGTACGCGAACTCATCAATCGCGTTCGACGAGCAATCATAATGTCGGAGGGATATTTAATCACCGCCGCAGA
TCTCGGGATCACTGATTTTG

Product: sigma-54 dependent DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MVVSSSAMQALFRTISKVAKSDASVFIFCEYGTGKELTATEIHKRSGRADSPFVVINCDAIPPQLLQSELFGYERGAFTG
ATHRKIGRLEEADGGTLFLDEIGDMQLESQVNLLRFLEERKLQRLGGRDSIMIGARIISATHCDIEQMIREGRFRSDLYH
RLYVLSIKEPPLHERDQDIELLAWHMLDRYKNDPGRRLHGLSADAVRALHSYD

Sequences:

>Translated_213_residues
MVVSSSAMQALFRTISKVAKSDASVFIFCEYGTGKELTATEIHKRSGRADSPFVVINCDAIPPQLLQSELFGYERGAFTG
ATHRKIGRLEEADGGTLFLDEIGDMQLESQVNLLRFLEERKLQRLGGRDSIMIGARIISATHCDIEQMIREGRFRSDLYH
RLYVLSIKEPPLHERDQDIELLAWHMLDRYKNDPGRRLHGLSADAVRALHSYD
>Mature_213_residues
MVVSSSAMQALFRTISKVAKSDASVFIFCEYGTGKELTATEIHKRSGRADSPFVVINCDAIPPQLLQSELFGYERGAFTG
ATHRKIGRLEEADGGTLFLDEIGDMQLESQVNLLRFLEERKLQRLGGRDSIMIGARIISATHCDIEQMIREGRFRSDLYH
RLYVLSIKEPPLHERDQDIELLAWHMLDRYKNDPGRRLHGLSADAVRALHSYD

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=213, Percent_Identity=49.2957746478873, Blast_Score=209, Evalue=9e-56,
Organism=Escherichia coli, GI1790437, Length=213, Percent_Identity=49.2957746478873, Blast_Score=202, Evalue=1e-53,
Organism=Escherichia coli, GI1790299, Length=187, Percent_Identity=51.3368983957219, Blast_Score=197, Evalue=4e-52,
Organism=Escherichia coli, GI1789233, Length=213, Percent_Identity=48.3568075117371, Blast_Score=195, Evalue=2e-51,
Organism=Escherichia coli, GI1788905, Length=211, Percent_Identity=49.2890995260664, Blast_Score=191, Evalue=3e-50,
Organism=Escherichia coli, GI1788550, Length=212, Percent_Identity=45.7547169811321, Blast_Score=191, Evalue=3e-50,
Organism=Escherichia coli, GI1789087, Length=209, Percent_Identity=46.88995215311, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI1786524, Length=198, Percent_Identity=48.4848484848485, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI87082152, Length=212, Percent_Identity=43.8679245283019, Blast_Score=170, Evalue=6e-44,
Organism=Escherichia coli, GI87081872, Length=179, Percent_Identity=44.6927374301676, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787583, Length=212, Percent_Identity=35.8490566037736, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1789828, Length=178, Percent_Identity=36.5168539325843, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI87081858, Length=166, Percent_Identity=33.7349397590361, Blast_Score=97, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 24151; Mature: 24151

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4 ; PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVSSSAMQALFRTISKVAKSDASVFIFCEYGTGKELTATEIHKRSGRADSPFVVINCDA
CCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEECCC
IPPQLLQSELFGYERGAFTGATHRKIGRLEEADGGTLFLDEIGDMQLESQVNLLRFLEER
CCHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
KLQRLGGRDSIMIGARIISATHCDIEQMIREGRFRSDLYHRLYVLSIKEPPLHERDQDIE
HHHHCCCCCCEEEHHHHHHHHCCCHHHHHHCCCHHHHHHHEEEEEEECCCCCCCCCCCHH
LLAWHMLDRYKNDPGRRLHGLSADAVRALHSYD
HHHHHHHHHHCCCCCCEECCCCHHHHHHHHCCC
>Mature Secondary Structure
MVVSSSAMQALFRTISKVAKSDASVFIFCEYGTGKELTATEIHKRSGRADSPFVVINCDA
CCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEECCC
IPPQLLQSELFGYERGAFTGATHRKIGRLEEADGGTLFLDEIGDMQLESQVNLLRFLEER
CCHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
KLQRLGGRDSIMIGARIISATHCDIEQMIREGRFRSDLYHRLYVLSIKEPPLHERDQDIE
HHHHCCCCCCEEEHHHHHHHHCCCHHHHHHCCCHHHHHHHEEEEEEECCCCCCCCCCCHH
LLAWHMLDRYKNDPGRRLHGLSADAVRALHSYD
HHHHHHHHHHCCCCCCEECCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]