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The map label for this gene is nisX1 [H]

Identifier: 76818153

GI number: 76818153

Start: 2362202

End: 2363590

Strand: Direct

Name: nisX1 [H]

Synonym: BURPS1710b_A1945

Alternate gene names: 76818153

Gene position: 2362202-2363590 (Clockwise)

Preceding gene: 76817487

Following gene: 76818573

Centisome position: 74.24

GC content: 59.97

Gene sequence:

>1389_bases
ATGTGCGCCCCGCCATTTTTTACCGGAGATACCAATCAAAACCGCTTCGAAGCTCATCGTCGCGGCCGTGCTGTTTCTCG
TGCTGCTGTCGATCGTCACCCCGTGGCTGGTAAATCAGGACAGCAGCATCACCCTGCTCGCCGTGCCGTTCGTGTGGCTG
GCATATGCCGCCGCCTTTGTGAAATTCATCCCCCCTCATTTCAAGGAGACCAAGTGAAACGCCTGTTTCTGATTCTGATC
CTCGTGCCGACGATGTTCCTCGCGGCCGGCTGCGATAACGTCCCGGCCGGCTACGTCGGTGTGAAGGTGCAACGCTACGG
CGACGACCGCGGCGTCAACGTCGAAGTGAAGGGGCCCGGGCGCTACTTCAACGGGCCCAACGTCGACATGTTCATCTTCC
CGACGTTCACGCAGTCCTACGTGTGGGACAAAGCTGGCAAGTCCGACGAGTCGTTCACGTTCCAGACGGTGGAGGGCCTG
TCGGTCAACACCGACATCGGCGTCAGCTACGCGATCCCGCGTGAGAACGCGCCCAAGGTGTTCCAGAAGTATCGGCGCGG
AGTAGATGAGATCACGGGGGTCTACCTGCGCGCGATCGTGCGCGACGCCCTGAATCTCGCCGGCGCGTCGATGGCGGTCG
AGGACGTCTACGGCAGGGGCAAGGCGGCACTACAGCAGCGCGTCGAGGACGAGGTAAAGGCGAACGCCGCGAAGGTCGGA
ATCAGTGTCGAGAAGGTCTATTTCGTGAATCAGATGCGCCTCCCCGAGCAGGTCATGAACTCGATCAACGGGAAGATCGC
AGCGACGCAGATCGCGCAGCAGAAGGAGAACGAACTGCGTGCAGCCGAGGCAGACGCGGCAAAGCAAGTCGCGATCGCCA
AGGGCGAAGCCGAAGCGCTCGAGGTGAAAGCGAAAGCACTACGCGAGAACAGCCAGATCCTGCAACAGATGGCGATCGAG
AAATGGGACGGCAAGCTTCCCCAGTACGCGCGCCGCGCGGTGGGCTGGGCGTTTAGTCAGCACGCGGACACCGACTTGGC
ACTAAAGGCATTAAGACGGGCTTACGATCATCGTCGTCCGCCACCAGGCCTGATGTTCCATTCGGATCAGGGCTGCCAAT
ACACGAGCACGCGCTTTCTCGCCGAGCTGCGCGCCCGTGGCACGATCCAGAGCATGAGTCGGCGCGGCAACTGTTGGGAC
AACGCGGTGGTCGAGCGCTTCTTCCGAAGCCTCAAAAATGAATGGATCGGCGACCAGTTGTACGTCGATCACCGCCATGC
AGAGCAAGACATCACTGATTATCTAGTCGATTTTTACAACCATCGTCGCCTTCACTCGGCTGCGAAGGGACTTCGACAGA
CGTATCGATTTGGTGTTCAAGGCCAGTGA

Upstream 100 bases:

>100_bases
GCGGATTTGTGGCAAACGCAAGCCACACACAGCAGTTCCCCGCTTGCCGCGCGCGGGATTTCCTCTCGGATAAGCGCGGC
CTTTCGGCGGGGCGGCCCGT

Downstream 100 bases:

>100_bases
AACCTGTCAGCGCATCGCGCAGATTGAAGGGGTTGGCCCCAAGACGGCTACCGCCATCGTTGCTGCAGTTTCTGACCCAA
AAGACTTCCGGAACGGCCGA

Product: bacteriophage/transposase fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MCAPPFFTGDTNQNRFEAHRRGRAVSRAAVDRHPVAGKSGQQHHPARRAVRVAGICRRLCEIHPPSFQGDQVKRLFLILI
LVPTMFLAAGCDNVPAGYVGVKVQRYGDDRGVNVEVKGPGRYFNGPNVDMFIFPTFTQSYVWDKAGKSDESFTFQTVEGL
SVNTDIGVSYAIPRENAPKVFQKYRRGVDEITGVYLRAIVRDALNLAGASMAVEDVYGRGKAALQQRVEDEVKANAAKVG
ISVEKVYFVNQMRLPEQVMNSINGKIAATQIAQQKENELRAAEADAAKQVAIAKGEAEALEVKAKALRENSQILQQMAIE
KWDGKLPQYARRAVGWAFSQHADTDLALKALRRAYDHRRPPPGLMFHSDQGCQYTSTRFLAELRARGTIQSMSRRGNCWD
NAVVERFFRSLKNEWIGDQLYVDHRHAEQDITDYLVDFYNHRRLHSAAKGLRQTYRFGVQGQ

Sequences:

>Translated_462_residues
MCAPPFFTGDTNQNRFEAHRRGRAVSRAAVDRHPVAGKSGQQHHPARRAVRVAGICRRLCEIHPPSFQGDQVKRLFLILI
LVPTMFLAAGCDNVPAGYVGVKVQRYGDDRGVNVEVKGPGRYFNGPNVDMFIFPTFTQSYVWDKAGKSDESFTFQTVEGL
SVNTDIGVSYAIPRENAPKVFQKYRRGVDEITGVYLRAIVRDALNLAGASMAVEDVYGRGKAALQQRVEDEVKANAAKVG
ISVEKVYFVNQMRLPEQVMNSINGKIAATQIAQQKENELRAAEADAAKQVAIAKGEAEALEVKAKALRENSQILQQMAIE
KWDGKLPQYARRAVGWAFSQHADTDLALKALRRAYDHRRPPPGLMFHSDQGCQYTSTRFLAELRARGTIQSMSRRGNCWD
NAVVERFFRSLKNEWIGDQLYVDHRHAEQDITDYLVDFYNHRRLHSAAKGLRQTYRFGVQGQ
>Mature_462_residues
MCAPPFFTGDTNQNRFEAHRRGRAVSRAAVDRHPVAGKSGQQHHPARRAVRVAGICRRLCEIHPPSFQGDQVKRLFLILI
LVPTMFLAAGCDNVPAGYVGVKVQRYGDDRGVNVEVKGPGRYFNGPNVDMFIFPTFTQSYVWDKAGKSDESFTFQTVEGL
SVNTDIGVSYAIPRENAPKVFQKYRRGVDEITGVYLRAIVRDALNLAGASMAVEDVYGRGKAALQQRVEDEVKANAAKVG
ISVEKVYFVNQMRLPEQVMNSINGKIAATQIAQQKENELRAAEADAAKQVAIAKGEAEALEVKAKALRENSQILQQMAIE
KWDGKLPQYARRAVGWAFSQHADTDLALKALRRAYDHRRPPPGLMFHSDQGCQYTSTRFLAELRARGTIQSMSRRGNCWD
NAVVERFFRSLKNEWIGDQLYVDHRHAEQDITDYLVDFYNHRRLHSAAKGLRQTYRFGVQGQ

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: COG0330

COG function: function code O; Membrane protease subunits, stomatin/prohibitin homologs

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1788405, Length=123, Percent_Identity=35.7723577235772, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787263, Length=123, Percent_Identity=35.7723577235772, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786753, Length=123, Percent_Identity=35.7723577235772, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786570, Length=123, Percent_Identity=35.7723577235772, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786490, Length=123, Percent_Identity=35.7723577235772, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789981, Length=119, Percent_Identity=35.2941176470588, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 51932; Mature: 51932

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAPPFFTGDTNQNRFEAHRRGRAVSRAAVDRHPVAGKSGQQHHPARRAVRVAGICRRLC
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EIHPPSFQGDQVKRLFLILILVPTMFLAAGCDNVPAGYVGVKVQRYGDDRGVNVEVKGPG
HCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCEEEEECCCC
RYFNGPNVDMFIFPTFTQSYVWDKAGKSDESFTFQTVEGLSVNTDIGVSYAIPRENAPKV
CCCCCCCEEEEEECCCHHHHHHHCCCCCCCCEEEEEECCCEECCCCCEEEECCCCCCHHH
FQKYRRGVDEITGVYLRAIVRDALNLAGASMAVEDVYGRGKAALQQRVEDEVKANAAKVG
HHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
ISVEKVYFVNQMRLPEQVMNSINGKIAATQIAQQKENELRAAEADAAKQVAIAKGEAEAL
CCHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
EVKAKALRENSQILQQMAIEKWDGKLPQYARRAVGWAFSQHADTDLALKALRRAYDHRRP
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
PPGLMFHSDQGCQYTSTRFLAELRARGTIQSMSRRGNCWDNAVVERFFRSLKNEWIGDQL
CCCCEEECCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCE
YVDHRHAEQDITDYLVDFYNHRRLHSAAKGLRQTYRFGVQGQ
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MCAPPFFTGDTNQNRFEAHRRGRAVSRAAVDRHPVAGKSGQQHHPARRAVRVAGICRRLC
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EIHPPSFQGDQVKRLFLILILVPTMFLAAGCDNVPAGYVGVKVQRYGDDRGVNVEVKGPG
HCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCEEEEECCCC
RYFNGPNVDMFIFPTFTQSYVWDKAGKSDESFTFQTVEGLSVNTDIGVSYAIPRENAPKV
CCCCCCCEEEEEECCCHHHHHHHCCCCCCCCEEEEEECCCEECCCCCEEEECCCCCCHHH
FQKYRRGVDEITGVYLRAIVRDALNLAGASMAVEDVYGRGKAALQQRVEDEVKANAAKVG
HHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
ISVEKVYFVNQMRLPEQVMNSINGKIAATQIAQQKENELRAAEADAAKQVAIAKGEAEAL
CCHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
EVKAKALRENSQILQQMAIEKWDGKLPQYARRAVGWAFSQHADTDLALKALRRAYDHRRP
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
PPGLMFHSDQGCQYTSTRFLAELRARGTIQSMSRRGNCWDNAVVERFFRSLKNEWIGDQL
CCCCEEECCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCE
YVDHRHAEQDITDYLVDFYNHRRLHSAAKGLRQTYRFGVQGQ
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1482189; 11337471; 1482192 [H]