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The map label for this gene is gcvA [H]

Identifier: 76818083

GI number: 76818083

Start: 1530680

End: 1531621

Strand: Direct

Name: gcvA [H]

Synonym: BURPS1710b_A1221

Alternate gene names: 76818083

Gene position: 1530680-1531621 (Clockwise)

Preceding gene: 76819263

Following gene: 76819508

Centisome position: 48.11

GC content: 70.17

Gene sequence:

>942_bases
ATGGATATCAAGCTCGATACGCCGTTGCTCGGCGCGCTGCGCTGCTTCGAAGCGGCGGGCCGGCTCGGCAGCTTCACGAA
GGCGGCCGACGCGCTGCACCTGACGCAAAGCGCGATCAGCCAGCAGATCCGCCAGCTCGAGGCGCGCCTCGGCTATCTGC
TGTTCGTGCGCCACGCGCGCGGGCTCGCGCTGACGCCGAAGGGCGGCGCGCTGCACGAAGCCACCGTGGCGGCATTCGCA
TCGCTCGGCCGCACGCTGCGGCAACTGAGCGCGCCCGACGCGCCGCTGCAGGTGAGCTGCCTGCCGTCGTTCGCGCTGCA
ATGGCTGATGCCGAGGCTGACCGAATTTCATCGGCTGCATCCGCAGACGCCGGTGCGGCTGAGGGCCGAGTTTCATGCGA
TCGATCGCGAGACGATGAGCGCGGACGACATCGATATCGCGATCCGCTGGGACCCGTTCAGCTATGGCACGCTCCACGTC
GAACCGCTGTTCGACGAATCGCTCGTCGCGGCCGCCGCGCCCGCGTATCTCGACCGGCATCCGCCGCGCGCGACGGCGAA
GTGGGTGTCGAGCGTCGTGCCGCTGCACGATGCGTCGGCGTGGCCGGAGGCGCCGAGGTATGTCGAATGGCGGACGTGGC
TCGATGCGGTCGTGCCCGGCTATGCGGGCGCGCCGCGGGGGCCGGAGTTCAATCTGGCGAGCCTGGCGATCGCGGCAGCG
GTTGCGGGGCAAGGGGTGGTGGTGTGCCGGCAGGCGCTCGTCGACGACGAAATCGCGCGTGGCCGGCTCGTCAATGTGTT
CGCGCGCCGCGTGCCCGCGCCGGCGCAATACAAGGCGATTTGCGCGCGGCCGGAAGACGCGCGGGTGAAAGCGTTTGTCG
GCTGGTTGCGGGCGGAATCGGACAGTTACGCGGCGCGGCGGCAAACGCAGGCGCGGCCGTAA

Upstream 100 bases:

>100_bases
GCATGATCTGAAATAAAATGTGATGGACAAGCCCTCATCGTACGGCTCCACGGGCCGTGCGAAGAAGCGATCAATTCTTG
CCCATGCATAAGCCACGCTA

Downstream 100 bases:

>100_bases
GCGCAGGCGCGGCGCGCGCCCCACGCGCGCGCGACTTGGGCCGCCGCCGTTCGATAGACAAGCATTGGATGGCGGATCGA
ACGCCGCGAAAAATCAGGTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MDIKLDTPLLGALRCFEAAGRLGSFTKAADALHLTQSAISQQIRQLEARLGYLLFVRHARGLALTPKGGALHEATVAAFA
SLGRTLRQLSAPDAPLQVSCLPSFALQWLMPRLTEFHRLHPQTPVRLRAEFHAIDRETMSADDIDIAIRWDPFSYGTLHV
EPLFDESLVAAAAPAYLDRHPPRATAKWVSSVVPLHDASAWPEAPRYVEWRTWLDAVVPGYAGAPRGPEFNLASLAIAAA
VAGQGVVVCRQALVDDEIARGRLVNVFARRVPAPAQYKAICARPEDARVKAFVGWLRAESDSYAARRQTQARP

Sequences:

>Translated_313_residues
MDIKLDTPLLGALRCFEAAGRLGSFTKAADALHLTQSAISQQIRQLEARLGYLLFVRHARGLALTPKGGALHEATVAAFA
SLGRTLRQLSAPDAPLQVSCLPSFALQWLMPRLTEFHRLHPQTPVRLRAEFHAIDRETMSADDIDIAIRWDPFSYGTLHV
EPLFDESLVAAAAPAYLDRHPPRATAKWVSSVVPLHDASAWPEAPRYVEWRTWLDAVVPGYAGAPRGPEFNLASLAIAAA
VAGQGVVVCRQALVDDEIARGRLVNVFARRVPAPAQYKAICARPEDARVKAFVGWLRAESDSYAARRQTQARP
>Mature_313_residues
MDIKLDTPLLGALRCFEAAGRLGSFTKAADALHLTQSAISQQIRQLEARLGYLLFVRHARGLALTPKGGALHEATVAAFA
SLGRTLRQLSAPDAPLQVSCLPSFALQWLMPRLTEFHRLHPQTPVRLRAEFHAIDRETMSADDIDIAIRWDPFSYGTLHV
EPLFDESLVAAAAPAYLDRHPPRATAKWVSSVVPLHDASAWPEAPRYVEWRTWLDAVVPGYAGAPRGPEFNLASLAIAAA
VAGQGVVVCRQALVDDEIARGRLVNVFARRVPAPAQYKAICARPEDARVKAFVGWLRAESDSYAARRQTQARP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=298, Percent_Identity=35.2348993288591, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=29.9319727891156, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786448, Length=262, Percent_Identity=35.4961832061069, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=24.6575342465753, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790262, Length=165, Percent_Identity=29.6969696969697, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI157672245, Length=189, Percent_Identity=31.2169312169312, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693193, Length=306, Percent_Identity=24.5098039215686, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789440, Length=181, Percent_Identity=28.1767955801105, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788887, Length=184, Percent_Identity=28.2608695652174, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34349; Mature: 34349

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKLDTPLLGALRCFEAAGRLGSFTKAADALHLTQSAISQQIRQLEARLGYLLFVRHAR
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
GLALTPKGGALHEATVAAFASLGRTLRQLSAPDAPLQVSCLPSFALQWLMPRLTEFHRLH
CEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCC
PQTPVRLRAEFHAIDRETMSADDIDIAIRWDPFSYGTLHVEPLFDESLVAAAAPAYLDRH
CCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHCC
PPRATAKWVSSVVPLHDASAWPEAPRYVEWRTWLDAVVPGYAGAPRGPEFNLASLAIAAA
CCHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
VAGQGVVVCRQALVDDEIARGRLVNVFARRVPAPAQYKAICARPEDARVKAFVGWLRAES
HCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHEECCCCCHHHHHHHHHHHCCC
DSYAARRQTQARP
CHHHHHHHHCCCC
>Mature Secondary Structure
MDIKLDTPLLGALRCFEAAGRLGSFTKAADALHLTQSAISQQIRQLEARLGYLLFVRHAR
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
GLALTPKGGALHEATVAAFASLGRTLRQLSAPDAPLQVSCLPSFALQWLMPRLTEFHRLH
CEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCC
PQTPVRLRAEFHAIDRETMSADDIDIAIRWDPFSYGTLHVEPLFDESLVAAAAPAYLDRH
CCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHCC
PPRATAKWVSSVVPLHDASAWPEAPRYVEWRTWLDAVVPGYAGAPRGPEFNLASLAIAAA
CCHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
VAGQGVVVCRQALVDDEIARGRLVNVFARRVPAPAQYKAICARPEDARVKAFVGWLRAES
HCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHEECCCCCHHHHHHHHHHHCCC
DSYAARRQTQARP
CHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]