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The map label for this gene is gcvA [H]

Identifier: 76819263

GI number: 76819263

Start: 1528178

End: 1529092

Strand: Direct

Name: gcvA [H]

Synonym: BURPS1710b_A1219

Alternate gene names: 76819263

Gene position: 1528178-1529092 (Clockwise)

Preceding gene: 76817976

Following gene: 76818083

Centisome position: 48.03

GC content: 72.35

Gene sequence:

>915_bases
ATGCCCGCCCGAAAGCCCGGCCGCCTGCCGCCGCTGAATGCGCTCCGCGCGTTCGAGGTGTCCGCGCGGCACCTGAACTT
CCGCGCGGCGGCCGACGAGATCGGCGTCACCCAGGGCGCGGTCGCGCAACAGGTCCGCCATCTGGAGGACGCGCTCGGCG
TGGCGCTGTTCGAACGCCTGCCGCGCGGCCTCGCGCTGACGGCCGACGGGCTCGCCTATTTCGCCGACGTGCGTCGTGCG
CTGAACCTGATCGGCGACGCGACGGACCGGCTCGCGAAGCGCCGCGCAAACGTCACGATCAGCACGACGCCCTCGTTCGC
GTCGCGCTGGCTGATTCCGCGGCTCGCGAGCTTCGGCGACGAGCATCCGGGCGTCGACCTTCGCGTGATCGCCGATTCGC
AGTTCGCGACCTTCCAGGGCGACGGCGTCGATCTCGCGATTCGCTACGGCAAGCCGCCGTTCGGCAAGGGGCTCGTCACG
CGCTTGCTGTTCCCGGTCGACATCTACGCGGTGTGCAGCCCCGCGCTGCTCGCCACCCCCGGCGTGCTCGCCCGCGCGCG
CGATCTCGCCGGGCACGCGCTGCTGCACGACGCGCATGACCTGTGGCCCGAGTTTCTCGCTGCGCTGCCCGAGCGCGTCG
CGATCGATCCGGCCAAGGGGCCGCGCTTCAATCAATCGTCGCTCGCCATCGACGCGGCGATCGCCGGGCACGGCGTCGCG
CTCGCGAGCGATCAGCTCGTCGCGCGCGACATCGAGGCCGGCCGGCTGCGGCGGCTGTTCGATTTCGCGCTGCCGCTGTC
GGTCGGCTATTACATCGTCCGTCCGAGCGAGCCGCGGCGCCCCGGCGACATCGCGCAGACCGAGGCGTGGCTGATCCGCC
AGGCGCAGCTGGATGCGCGACGCGACGGCGCGTAG

Upstream 100 bases:

>100_bases
CGGCAACCCCCGCTGTCGAATCATCGTTTTTGATGTCAGGCGATAGTTTTTCTCCTGTTTATTCGATTTCCGCGATGTCC
ACGATTTGTCCCGCTTCGAC

Downstream 100 bases:

>100_bases
CGCGCGCGGGCCGCGTTCGTCGCGGGGCGTTCGGATCGGTCGAGGGCGCCGCGCCGCGCGGGCGGCGTCTCTGTCTCTGT
CTCTGTCTCCGTCTTCGCCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MPARKPGRLPPLNALRAFEVSARHLNFRAAADEIGVTQGAVAQQVRHLEDALGVALFERLPRGLALTADGLAYFADVRRA
LNLIGDATDRLAKRRANVTISTTPSFASRWLIPRLASFGDEHPGVDLRVIADSQFATFQGDGVDLAIRYGKPPFGKGLVT
RLLFPVDIYAVCSPALLATPGVLARARDLAGHALLHDAHDLWPEFLAALPERVAIDPAKGPRFNQSSLAIDAAIAGHGVA
LASDQLVARDIEAGRLRRLFDFALPLSVGYYIVRPSEPRRPGDIAQTEAWLIRQAQLDARRDGA

Sequences:

>Translated_304_residues
MPARKPGRLPPLNALRAFEVSARHLNFRAAADEIGVTQGAVAQQVRHLEDALGVALFERLPRGLALTADGLAYFADVRRA
LNLIGDATDRLAKRRANVTISTTPSFASRWLIPRLASFGDEHPGVDLRVIADSQFATFQGDGVDLAIRYGKPPFGKGLVT
RLLFPVDIYAVCSPALLATPGVLARARDLAGHALLHDAHDLWPEFLAALPERVAIDPAKGPRFNQSSLAIDAAIAGHGVA
LASDQLVARDIEAGRLRRLFDFALPLSVGYYIVRPSEPRRPGDIAQTEAWLIRQAQLDARRDGA
>Mature_303_residues
PARKPGRLPPLNALRAFEVSARHLNFRAAADEIGVTQGAVAQQVRHLEDALGVALFERLPRGLALTADGLAYFADVRRAL
NLIGDATDRLAKRRANVTISTTPSFASRWLIPRLASFGDEHPGVDLRVIADSQFATFQGDGVDLAIRYGKPPFGKGLVTR
LLFPVDIYAVCSPALLATPGVLARARDLAGHALLHDAHDLWPEFLAALPERVAIDPAKGPRFNQSSLAIDAAIAGHGVAL
ASDQLVARDIEAGRLRRLFDFALPLSVGYYIVRPSEPRRPGDIAQTEAWLIRQAQLDARRDGA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=41.2371134020619, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1786448, Length=306, Percent_Identity=32.0261437908497, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1788706, Length=273, Percent_Identity=28.9377289377289, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI145693193, Length=258, Percent_Identity=25.5813953488372, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786401, Length=259, Percent_Identity=25.8687258687259, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081978, Length=280, Percent_Identity=26.4285714285714, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787589, Length=274, Percent_Identity=25.1824817518248, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789639, Length=241, Percent_Identity=25.3112033195021, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787128, Length=264, Percent_Identity=21.5909090909091, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI157672245, Length=121, Percent_Identity=34.7107438016529, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32919; Mature: 32788

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARKPGRLPPLNALRAFEVSARHLNFRAAADEIGVTQGAVAQQVRHLEDALGVALFERL
CCCCCCCCCCCHHHHHHHHHHHHHCEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
PRGLALTADGLAYFADVRRALNLIGDATDRLAKRRANVTISTTPSFASRWLIPRLASFGD
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHCCC
EHPGVDLRVIADSQFATFQGDGVDLAIRYGKPPFGKGLVTRLLFPVDIYAVCSPALLATP
CCCCCEEEEEECCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHCC
GVLARARDLAGHALLHDAHDLWPEFLAALPERVAIDPAKGPRFNQSSLAIDAAIAGHGVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCCCHHEEEHHHCCCCEE
LASDQLVARDIEAGRLRRLFDFALPLSVGYYIVRPSEPRRPGDIAQTEAWLIRQAQLDAR
ECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHC
RDGA
CCCC
>Mature Secondary Structure 
PARKPGRLPPLNALRAFEVSARHLNFRAAADEIGVTQGAVAQQVRHLEDALGVALFERL
CCCCCCCCCCHHHHHHHHHHHHHCEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
PRGLALTADGLAYFADVRRALNLIGDATDRLAKRRANVTISTTPSFASRWLIPRLASFGD
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHCCC
EHPGVDLRVIADSQFATFQGDGVDLAIRYGKPPFGKGLVTRLLFPVDIYAVCSPALLATP
CCCCCEEEEEECCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHCC
GVLARARDLAGHALLHDAHDLWPEFLAALPERVAIDPAKGPRFNQSSLAIDAAIAGHGVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCCCHHEEEHHHCCCCEE
LASDQLVARDIEAGRLRRLFDFALPLSVGYYIVRPSEPRRPGDIAQTEAWLIRQAQLDAR
ECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHC
RDGA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]