Click here to switch to the map view.

The map label for this gene is lgrC [H]

Identifier: 76817356

GI number: 76817356

Start: 193375

End: 194892

Strand: Direct

Name: lgrC [H]

Synonym: BURPS1710b_A0146

Alternate gene names: 76817356

Gene position: 193375-194892 (Clockwise)

Preceding gene: 76819215

Following gene: 76818046

Centisome position: 6.08

GC content: 70.69

Gene sequence:

>1518_bases
GTGACCTCAACCAGCGTCAATCGTCTGTTCACTTCGCAAGCCCGCCTTGCGCCCGAAGCGCTCGCGCTCTCGAGCGGCGA
CACGCGCCTCACGTACGGCGAGCTCGAACGATGCGCGAACCACCTGGCCCGACGCCTCGTCGACAGCGGCGTGCGGCCGC
GCGACCGGGTCCTGCTCTGCCTGCCGCGCTCGGTCGACGCGGTGATCGCGATGCTCGCGATCATGAAGACCGGCGCGGCG
TTCGTGCCGGTCGATCCCGCGTATTCCGACGCGATCAAGCGCGGCTATGCGAGCGACAGCGGCGCGCGGCACGCGCTCGC
GCGCGCGGCCGACGCCGCGGCGTTTCGCGGCGGCGCGCTGGGCGTGATCGACGCCGACGATCTGTCGGCCGCACGCGATG
ACGAGGGGCCCGAAGTCGATGCGGGGCACGACGGGGAAACGCCGGTGTACGTGATGTTCACCTCCGGCAGCACCGGCCGG
CCCAAGGGCGTGATCGTCGCGCACCGCGGCGTCGCGCGGCTCGTCAGGGAAACGAACTATATCCGGATCACGCGCGAGGA
CACGCTGCTGCTGCTCTCGCCGATCACGTTCGACGCATCGACGTTCGAGATCTGGGGGGCGCTGCTCAACGGCGCGCGGC
TCGCGATCTACGAGGACGCCACGTTCGATCCGAACGCCGTCAGCCGGCTCATCGCGCGCGAGCAAGTAAGCGTGATGTGG
CTCACCGCGGGGCTGTTCCATCTGGTCGCGCGGCGCTTCGTCGGCATGCTGGCGGGGCTGCGCGTCGTGCTCGCTGGCGG
CGACGTGCTGAGCGCCGCCGCGATCGGCGCGGTGTTCGACGCGTTCCCGTCGATCACCGTCATCAACGGCTACGGCCCGA
CCGAGAACACGACGTTCACGTGCTGCCACGTGATGACGGCCGACCGGCGGCCGACCGGTACGGTGCCGATCGGCCGGCCG
ATCGCGGGCACCGACGTTCGCATTCTCGACGCCGCGCTGCGCGAGGTGCCTGACGGCGAGGAAGGCGAGCTGTGCGCAAG
CGGCCTCGGCGTCGCGCTCGGCTACCTGAACGCGCCCGACGCGACGCGCGCCGCGTTCGTCGACTGCCCGGCGACGGGCA
GCCGGCTCTATCGCACCGGCGACCGCGCGCGGCGCCGGGCGGACGGCGTGATCGAGTTCCTCGGCCGCAGCGACCGGCTC
GTGAAGATACGCGGCTACCGCGTGTCGCTCGACGCGCTGCAATCCGTCCTCGCCGGCATTCCCGGCGTCGAGGAGGCGCT
CGTCAAGGTATCCGAAGAAGCGACCGGCGAGAAGCGCCTCAGCGCGATCGTCCAATCCGGCCGCGCCGAACCGGACATGA
AGGCCTACGTGCGCCGCGAACTGGCCAAGCGCGTGCCGCCGTTCCAGATTCCCGACGACATCCGGATTTTCCCGCACATC
CCGCTCAACGCGAACGGCAAGCTCGACCGCCACCGGCTGCCGGTCAGCGAGACCTCGACCCTCGGAGAGAAGCCATGA

Upstream 100 bases:

>100_bases
ATTCGGCCGCCGGCGCGCTGCGCCATTCGTGAGCGGCCGACGCCGCGCGCCATCGCCCCCGGCCCGGCGGGCATCAGCTT
TCCTGAACGCGAGAGAATCC

Downstream 100 bases:

>100_bases
CCCACACGATAGACATCACCGAAACGATTCACAACACCTGCCGCAGCGTGCTCGGGATTCCCGATCTTCAGTCCGACGAA
GATTTCTTCGAGCGCGGCGT

Product: nonribosomal peptide synthetase

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: ATP-dependent valine adenylase; ValA; Valine activase; ATP-dependent D-valine adenylase; D-ValA; D-valine activase; Valine racemase [ATP-hydrolyzing]; ATP-dependent tryptophan adenylase; TrpA; Tryptophan activase; ATP-dependent D-leucine adenylase; D-LeuA; D-leucine activase; Leucine racemase [ATP-hydrolyzing]; ATP-dependent tryptophan/phenylalanine/tyrosine adenylase; Trp/Phe/TyrA; Tryptophan/phenylalanine/tyrosine activase; ATP-dependent D-leucine adenylase; D-LeuA; D-leucine activase; Leucine racemase [ATP-hydrolyzing] [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MTSTSVNRLFTSQARLAPEALALSSGDTRLTYGELERCANHLARRLVDSGVRPRDRVLLCLPRSVDAVIAMLAIMKTGAA
FVPVDPAYSDAIKRGYASDSGARHALARAADAAAFRGGALGVIDADDLSAARDDEGPEVDAGHDGETPVYVMFTSGSTGR
PKGVIVAHRGVARLVRETNYIRITREDTLLLLSPITFDASTFEIWGALLNGARLAIYEDATFDPNAVSRLIAREQVSVMW
LTAGLFHLVARRFVGMLAGLRVVLAGGDVLSAAAIGAVFDAFPSITVINGYGPTENTTFTCCHVMTADRRPTGTVPIGRP
IAGTDVRILDAALREVPDGEEGELCASGLGVALGYLNAPDATRAAFVDCPATGSRLYRTGDRARRRADGVIEFLGRSDRL
VKIRGYRVSLDALQSVLAGIPGVEEALVKVSEEATGEKRLSAIVQSGRAEPDMKAYVRRELAKRVPPFQIPDDIRIFPHI
PLNANGKLDRHRLPVSETSTLGEKP

Sequences:

>Translated_505_residues
MTSTSVNRLFTSQARLAPEALALSSGDTRLTYGELERCANHLARRLVDSGVRPRDRVLLCLPRSVDAVIAMLAIMKTGAA
FVPVDPAYSDAIKRGYASDSGARHALARAADAAAFRGGALGVIDADDLSAARDDEGPEVDAGHDGETPVYVMFTSGSTGR
PKGVIVAHRGVARLVRETNYIRITREDTLLLLSPITFDASTFEIWGALLNGARLAIYEDATFDPNAVSRLIAREQVSVMW
LTAGLFHLVARRFVGMLAGLRVVLAGGDVLSAAAIGAVFDAFPSITVINGYGPTENTTFTCCHVMTADRRPTGTVPIGRP
IAGTDVRILDAALREVPDGEEGELCASGLGVALGYLNAPDATRAAFVDCPATGSRLYRTGDRARRRADGVIEFLGRSDRL
VKIRGYRVSLDALQSVLAGIPGVEEALVKVSEEATGEKRLSAIVQSGRAEPDMKAYVRRELAKRVPPFQIPDDIRIFPHI
PLNANGKLDRHRLPVSETSTLGEKP
>Mature_504_residues
TSTSVNRLFTSQARLAPEALALSSGDTRLTYGELERCANHLARRLVDSGVRPRDRVLLCLPRSVDAVIAMLAIMKTGAAF
VPVDPAYSDAIKRGYASDSGARHALARAADAAAFRGGALGVIDADDLSAARDDEGPEVDAGHDGETPVYVMFTSGSTGRP
KGVIVAHRGVARLVRETNYIRITREDTLLLLSPITFDASTFEIWGALLNGARLAIYEDATFDPNAVSRLIAREQVSVMWL
TAGLFHLVARRFVGMLAGLRVVLAGGDVLSAAAIGAVFDAFPSITVINGYGPTENTTFTCCHVMTADRRPTGTVPIGRPI
AGTDVRILDAALREVPDGEEGELCASGLGVALGYLNAPDATRAAFVDCPATGSRLYRTGDRARRRADGVIEFLGRSDRLV
KIRGYRVSLDALQSVLAGIPGVEEALVKVSEEATGEKRLSAIVQSGRAEPDMKAYVRRELAKRVPPFQIPDDIRIFPHIP
LNANGKLDRHRLPVSETSTLGEKP

Specific function: Activates the 7th to 12th amino acids (Val, D-Val, Trp, D-Leu, Xaa and D-Leu) in linear gramicidin and catalyzes the formation of the peptide bond between them. This enzyme is also responsible for the epimerization of the 8th (D-Val), the 10th (D- Leu) an

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 6 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI45580730, Length=508, Percent_Identity=26.5748031496063, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI42544132, Length=511, Percent_Identity=22.8962818003914, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI38505220, Length=477, Percent_Identity=24.3186582809224, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI156151445, Length=553, Percent_Identity=23.5081374321881, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI115511026, Length=502, Percent_Identity=23.3067729083665, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI157311624, Length=527, Percent_Identity=21.8216318785579, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI157311622, Length=527, Percent_Identity=21.8216318785579, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI122937307, Length=518, Percent_Identity=22.2007722007722, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI58082049, Length=530, Percent_Identity=21.8867924528302, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1786801, Length=514, Percent_Identity=32.8793774319066, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI145693145, Length=532, Percent_Identity=24.2481203007519, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788107, Length=544, Percent_Identity=22.9779411764706, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1790505, Length=543, Percent_Identity=21.3627992633518, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786810, Length=519, Percent_Identity=24.8554913294798, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789201, Length=357, Percent_Identity=28.2913165266106, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17560140, Length=508, Percent_Identity=25.9842519685039, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17556356, Length=472, Percent_Identity=25, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=546, Percent_Identity=24.9084249084249, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17550940, Length=347, Percent_Identity=25.6484149855908, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17557194, Length=505, Percent_Identity=22.970297029703, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI32563687, Length=509, Percent_Identity=23.1827111984283, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI32564420, Length=445, Percent_Identity=24.7191011235955, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI32564422, Length=445, Percent_Identity=24.7191011235955, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=362, Percent_Identity=23.7569060773481, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71983001, Length=536, Percent_Identity=24.2537313432836, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71982997, Length=536, Percent_Identity=24.2537313432836, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71985884, Length=358, Percent_Identity=22.3463687150838, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319591, Length=583, Percent_Identity=23.4991423670669, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6319699, Length=527, Percent_Identity=21.8216318785579, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648676, Length=544, Percent_Identity=28.6764705882353, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI18859661, Length=517, Percent_Identity=26.3056092843327, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24582852, Length=453, Percent_Identity=24.9448123620309, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI62472339, Length=392, Percent_Identity=26.530612244898, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24667959, Length=392, Percent_Identity=26.530612244898, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24667955, Length=392, Percent_Identity=26.530612244898, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI19922652, Length=314, Percent_Identity=23.8853503184713, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24581924, Length=526, Percent_Identity=23.7642585551331, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648260, Length=518, Percent_Identity=23.5521235521236, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21356441, Length=518, Percent_Identity=21.8146718146718, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21358303, Length=351, Percent_Identity=21.6524216524217, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21355181, Length=499, Percent_Identity=23.8476953907816, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR023213
- InterPro:   IPR001242
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 54081; Mature: 53950

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTSVNRLFTSQARLAPEALALSSGDTRLTYGELERCANHLARRLVDSGVRPRDRVLLC
CCCCHHHHHHHHHHHCCCHHHEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
LPRSVDAVIAMLAIMKTGAAFVPVDPAYSDAIKRGYASDSGARHALARAADAAAFRGGAL
ECCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEE
GVIDADDLSAARDDEGPEVDAGHDGETPVYVMFTSGSTGRPKGVIVAHRGVARLVRETNY
EEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECHHHHHHHHCCCE
IRITREDTLLLLSPITFDASTFEIWGALLNGARLAIYEDATFDPNAVSRLIAREQVSVMW
EEEECCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
LTAGLFHLVARRFVGMLAGLRVVLAGGDVLSAAAIGAVFDAFPSITVINGYGPTENTTFT
HHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEE
CCHVMTADRRPTGTVPIGRPIAGTDVRILDAALREVPDGEEGELCASGLGVALGYLNAPD
EEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCC
ATRAAFVDCPATGSRLYRTGDRARRRADGVIEFLGRSDRLVKIRGYRVSLDALQSVLAGI
CCCEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECEEEEHHHHHHHHHCC
PGVEEALVKVSEEATGEKRLSAIVQSGRAEPDMKAYVRRELAKRVPPFQIPDDIRIFPHI
CCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECC
PLNANGKLDRHRLPVSETSTLGEKP
CCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSTSVNRLFTSQARLAPEALALSSGDTRLTYGELERCANHLARRLVDSGVRPRDRVLLC
CCCHHHHHHHHHHHCCCHHHEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
LPRSVDAVIAMLAIMKTGAAFVPVDPAYSDAIKRGYASDSGARHALARAADAAAFRGGAL
ECCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEE
GVIDADDLSAARDDEGPEVDAGHDGETPVYVMFTSGSTGRPKGVIVAHRGVARLVRETNY
EEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECHHHHHHHHCCCE
IRITREDTLLLLSPITFDASTFEIWGALLNGARLAIYEDATFDPNAVSRLIAREQVSVMW
EEEECCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
LTAGLFHLVARRFVGMLAGLRVVLAGGDVLSAAAIGAVFDAFPSITVINGYGPTENTTFT
HHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEE
CCHVMTADRRPTGTVPIGRPIAGTDVRILDAALREVPDGEEGELCASGLGVALGYLNAPD
EEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCC
ATRAAFVDCPATGSRLYRTGDRARRRADGVIEFLGRSDRLVKIRGYRVSLDALQSVLAGI
CCCEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECEEEEHHHHHHHHHCC
PGVEEALVKVSEEATGEKRLSAIVQSGRAEPDMKAYVRRELAKRVPPFQIPDDIRIFPHI
CCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECC
PLNANGKLDRHRLPVSETSTLGEKP
CCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA