Click here to switch to the map view.

The map label for this gene is tnsB [H]

Identifier: 75909693

GI number: 75909693

Start: 4325002

End: 4327230

Strand: Direct

Name: tnsB [H]

Synonym: Ava_3487

Alternate gene names: 75909693

Gene position: 4325002-4327230 (Clockwise)

Preceding gene: 75909692

Following gene: 75909694

Centisome position: 67.94

GC content: 34.28

Gene sequence:

>2229_bases
ATGGTTAAAGATTTATTTGTCAATGATTTAATAGAGTGGATTGTATACCAAAACACTCATATAGTCGAAAGAATTATTTG
GATAGATGAAGGTTACATTATTGCATTTGTTATAGATATTAATGCTAAGAAAGGTCTACCTGAACCAAGGAAAATATCAG
ATATTTTAGAAGCTATAACTGAAGGTATTGCTTTTAAGCAGCAACAAGATCCTTGGGCAAGAATTATTCGGGATGAAAAT
TTGACAGATAAGGAAAAGGAATACAGAGATAAAGCTTGGGAGATAATTTCATCATTAATTATAGAAGAACCATCAATATA
TTATAGAAATTTTAGAGGATCGATGGTTAAAGAAGCGGTTGAAAAGTACAACGAAGAAAGGGATAAAGATAAATTAGTAG
AAAAAACCGTCTATGGTTATTTGAGAAGATTTTGGCAGAGAGGAAAAACTATAAATGCTCTCATACCTGATTTTATCAAT
TCATCAGGTAAGGGCAAGATTCGAGGACAATCGGGCAAGAAGAGAGGAAGACCAAGAAAATATGCACATGACCCAGATAT
TGGTGAAGGTGTAAATGTTACAGAAGAAGATAGAAAGATTTTTAGAGTATCTATAAGCAAATTTTACAATAATCCAAAAG
AAAACTACTTAACAACAGCCTATGAATTAATGGTGAAAGAATATTACAAGGAAGGAATTAGATATGACGAGAATGGTGTT
CAAAAATCTATATTAACCCCAACTGATAAAATACCTACAATTACTCAATTCAAATACTGGTATCAACTTGAACAGGATGA
TGTTACCAAAACTTTAACATCTCGAAAAGGAGCCAAGCGAGTAGCTTTAGAGAATAGAGCAATTTTGGGAAACTCTAAGA
TGGAAACTATTGGCCCTGGTTCTAGATATCAAATTGATGCCACAGTTGCTGATGTATATTTAGTTTCAAAATATAACCGT
GACTGGATAATTGGTAGACCAGTTATCTATGTAATTATTGATGTTTTTAGTAGAATGATTACAGGTGTTTATGTCGGTTT
AGAAGGCCCATCTTGGATAGGAGCAATGATGGCACTAGCCAATGTAGCTACAGATAAAACCCTGTTTTGCCAGGAATATA
ACATTAATATTACTGAAAATGAATGGCCTTGCAATCAAATGCCAGATGCAATTTTAGGAGATAGGGGAGAATTAGCAGGT
ATGACAGTAGAAACATTAATCCCTAATCTTTATGTTCGTATTGAAAATGCAGCTTCCTATAGAGCAGATTGGAAAGGGTT
AGTAGAAAGACACTTTCGTATAATTCATGAATATGTAAAACCTTTTTTACCTGGTTATGTGGATACGGATTTTAGGCAAA
GAGGTGCAAGGGATTATAGGCTTGATGGCAAACTTGATATAGATCAATTTACTGAGATTATTATTCGTTTAATTGTTCAC
CATAACAATAAACCTCTTACTGAATATGAAAGAGATGAAGCAATGATTGCTGATGATACCCCTCCTATACCTAGAGAGTT
GTGGAAGTGGGGTATCGCCAATCGTTCTGGCAGACTCAGAACTTTTTCTGAAGATATTGTCAAATTGAACTTGATGCCTA
CTGAGAAAGCAACTATTACTGCTAAAGGCATCAAAATGAGAGGTAAGGAGATGTACTATACCTGTGATAGTGCTAAAAAT
GAGCAATGGTTTGAAAAAGCTAGAAGTAATCTCCTTTCTAAGTCAGAAAAATCATTGATTGTATCTTATGACATCAGAAA
GCCAAACTTCATTTATCTTCCATCTAGTGATGGTAGAGATTTTGAAAAATGTTTTCTTATTGACTTAGAAGGGCGGTATG
CTAATAAAAACTTTCATGATATTGAATATCTGCTTGCTTATGAAGAATTGCAAAGACAGAAAAGCCAAGGTAAACAGCTA
CAAGAAAAGGTAGATTTAATAGCTGATATTGAAAGCATAGTAGATAGAGCAGAAAAAATGACTGATGCTTCTCAATATCA
TAATTTAAGTAAGAGCAAGAAAGTTTCTGGAATAAGAGATAATAGGGCTTTTGAAAAGGCTAAACGACGAGAGAATGAAG
GATTTGAATTAGCAATTACAGATAATCAGAGCAGTTTTCAGGTTGCAGAGAGTACAGCAGAAACCTCCGAACAGCCAGAA
TCACTACAGCCAAACCATCTGAATCTTCTTAAGAAAAAGCGACAGGAGCGAAAACGTGGACAGAATTGA

Upstream 100 bases:

>100_bases
GAAATCCTTGTGGATCTAGTAGCAATAAAATTATCTGCTTGTCCGTCAACTAAAGTTATTGAAAAAATAATATTTTAGAC
CCACATGATAGGTAAATAAT

Downstream 100 bases:

>100_bases
GAGTGAACTGGTTAAAATTCCTAATGGAGAACTAGCAGTTATTGCCAGATATATAGATCAAAAACTGCCAGAATACAACA
CTAATCCATTAATTCAGGCA

Product: DNA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 742; Mature: 742

Protein sequence:

>742_residues
MVKDLFVNDLIEWIVYQNTHIVERIIWIDEGYIIAFVIDINAKKGLPEPRKISDILEAITEGIAFKQQQDPWARIIRDEN
LTDKEKEYRDKAWEIISSLIIEEPSIYYRNFRGSMVKEAVEKYNEERDKDKLVEKTVYGYLRRFWQRGKTINALIPDFIN
SSGKGKIRGQSGKKRGRPRKYAHDPDIGEGVNVTEEDRKIFRVSISKFYNNPKENYLTTAYELMVKEYYKEGIRYDENGV
QKSILTPTDKIPTITQFKYWYQLEQDDVTKTLTSRKGAKRVALENRAILGNSKMETIGPGSRYQIDATVADVYLVSKYNR
DWIIGRPVIYVIIDVFSRMITGVYVGLEGPSWIGAMMALANVATDKTLFCQEYNINITENEWPCNQMPDAILGDRGELAG
MTVETLIPNLYVRIENAASYRADWKGLVERHFRIIHEYVKPFLPGYVDTDFRQRGARDYRLDGKLDIDQFTEIIIRLIVH
HNNKPLTEYERDEAMIADDTPPIPRELWKWGIANRSGRLRTFSEDIVKLNLMPTEKATITAKGIKMRGKEMYYTCDSAKN
EQWFEKARSNLLSKSEKSLIVSYDIRKPNFIYLPSSDGRDFEKCFLIDLEGRYANKNFHDIEYLLAYEELQRQKSQGKQL
QEKVDLIADIESIVDRAEKMTDASQYHNLSKSKKVSGIRDNRAFEKAKRRENEGFELAITDNQSSFQVAESTAETSEQPE
SLQPNHLNLLKKKRQERKRGQN

Sequences:

>Translated_742_residues
MVKDLFVNDLIEWIVYQNTHIVERIIWIDEGYIIAFVIDINAKKGLPEPRKISDILEAITEGIAFKQQQDPWARIIRDEN
LTDKEKEYRDKAWEIISSLIIEEPSIYYRNFRGSMVKEAVEKYNEERDKDKLVEKTVYGYLRRFWQRGKTINALIPDFIN
SSGKGKIRGQSGKKRGRPRKYAHDPDIGEGVNVTEEDRKIFRVSISKFYNNPKENYLTTAYELMVKEYYKEGIRYDENGV
QKSILTPTDKIPTITQFKYWYQLEQDDVTKTLTSRKGAKRVALENRAILGNSKMETIGPGSRYQIDATVADVYLVSKYNR
DWIIGRPVIYVIIDVFSRMITGVYVGLEGPSWIGAMMALANVATDKTLFCQEYNINITENEWPCNQMPDAILGDRGELAG
MTVETLIPNLYVRIENAASYRADWKGLVERHFRIIHEYVKPFLPGYVDTDFRQRGARDYRLDGKLDIDQFTEIIIRLIVH
HNNKPLTEYERDEAMIADDTPPIPRELWKWGIANRSGRLRTFSEDIVKLNLMPTEKATITAKGIKMRGKEMYYTCDSAKN
EQWFEKARSNLLSKSEKSLIVSYDIRKPNFIYLPSSDGRDFEKCFLIDLEGRYANKNFHDIEYLLAYEELQRQKSQGKQL
QEKVDLIADIESIVDRAEKMTDASQYHNLSKSKKVSGIRDNRAFEKAKRRENEGFELAITDNQSSFQVAESTAETSEQPE
SLQPNHLNLLKKKRQERKRGQN
>Mature_742_residues
MVKDLFVNDLIEWIVYQNTHIVERIIWIDEGYIIAFVIDINAKKGLPEPRKISDILEAITEGIAFKQQQDPWARIIRDEN
LTDKEKEYRDKAWEIISSLIIEEPSIYYRNFRGSMVKEAVEKYNEERDKDKLVEKTVYGYLRRFWQRGKTINALIPDFIN
SSGKGKIRGQSGKKRGRPRKYAHDPDIGEGVNVTEEDRKIFRVSISKFYNNPKENYLTTAYELMVKEYYKEGIRYDENGV
QKSILTPTDKIPTITQFKYWYQLEQDDVTKTLTSRKGAKRVALENRAILGNSKMETIGPGSRYQIDATVADVYLVSKYNR
DWIIGRPVIYVIIDVFSRMITGVYVGLEGPSWIGAMMALANVATDKTLFCQEYNINITENEWPCNQMPDAILGDRGELAG
MTVETLIPNLYVRIENAASYRADWKGLVERHFRIIHEYVKPFLPGYVDTDFRQRGARDYRLDGKLDIDQFTEIIIRLIVH
HNNKPLTEYERDEAMIADDTPPIPRELWKWGIANRSGRLRTFSEDIVKLNLMPTEKATITAKGIKMRGKEMYYTCDSAKN
EQWFEKARSNLLSKSEKSLIVSYDIRKPNFIYLPSSDGRDFEKCFLIDLEGRYANKNFHDIEYLLAYEELQRQKSQGKQL
QEKVDLIADIESIVDRAEKMTDASQYHNLSKSKKVSGIRDNRAFEKAKRRENEGFELAITDNQSSFQVAESTAETSEQPE
SLQPNHLNLLKKKRQERKRGQN

Specific function: Sequence-specific, DNA-binding protein required for Tn7 transposition. Recognizes sequences necessary for recombination at both left and right ends of Tn7 and, together with tnsA, forms the transposase. TnsB executes the 5'-DNA strand breakage and joining

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 86414; Mature: 86414

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKDLFVNDLIEWIVYQNTHIVERIIWIDEGYIIAFVIDINAKKGLPEPRKISDILEAIT
CCHHHHHHHHHHHHHHCCCHHHHHEEEEECCEEEEEEEEECCCCCCCCCHHHHHHHHHHH
EGIAFKQQQDPWARIIRDENLTDKEKEYRDKAWEIISSLIIEEPSIYYRNFRGSMVKEAV
HHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHH
EKYNEERDKDKLVEKTVYGYLRRFWQRGKTINALIPDFINSSGKGKIRGQSGKKRGRPRK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHCCCCCCEEECCCCCCCCCCCC
YAHDPDIGEGVNVTEEDRKIFRVSISKFYNNPKENYLTTAYELMVKEYYKEGIRYDENGV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QKSILTPTDKIPTITQFKYWYQLEQDDVTKTLTSRKGAKRVALENRAILGNSKMETIGPG
HHHCCCCCCCCCCHHEEHEEEEECHHHHHHHHHHCCCCHHHHHCCCEEECCCCCCCCCCC
SRYQIDATVADVYLVSKYNRDWIIGRPVIYVIIDVFSRMITGVYVGLEGPSWIGAMMALA
CCEEEEHHHHHEEEEEECCCCEEECCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHH
NVATDKTLFCQEYNINITENEWPCNQMPDAILGDRGELAGMTVETLIPNLYVRIENAASY
HHCCCCEEEEEEECCEEECCCCCCCCCCHHHCCCCCCCCCCCHHHHCCCEEEEEECCCHH
RADWKGLVERHFRIIHEYVKPFLPGYVDTDFRQRGARDYRLDGKLDIDQFTEIIIRLIVH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEECCCCCCHHHHHHHHHHHHHH
HNNKPLTEYERDEAMIADDTPPIPRELWKWGIANRSGRLRTFSEDIVKLNLMPTEKATIT
CCCCCCCHHCCCCCEECCCCCCCCHHHHHHCCCCCCCCCEECCCCCEEEEEECCCCCEEE
AKGIKMRGKEMYYTCDSAKNEQWFEKARSNLLSKSEKSLIVSYDIRKPNFIYLPSSDGRD
ECCCEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCCCC
FEKCFLIDLEGRYANKNFHDIEYLLAYEELQRQKSQGKQLQEKVDLIADIESIVDRAEKM
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDASQYHNLSKSKKVSGIRDNRAFEKAKRRENEGFELAITDNQSSFQVAESTAETSEQPE
HHHHHHHCCCHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCC
SLQPNHLNLLKKKRQERKRGQN
CCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVKDLFVNDLIEWIVYQNTHIVERIIWIDEGYIIAFVIDINAKKGLPEPRKISDILEAIT
CCHHHHHHHHHHHHHHCCCHHHHHEEEEECCEEEEEEEEECCCCCCCCCHHHHHHHHHHH
EGIAFKQQQDPWARIIRDENLTDKEKEYRDKAWEIISSLIIEEPSIYYRNFRGSMVKEAV
HHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHH
EKYNEERDKDKLVEKTVYGYLRRFWQRGKTINALIPDFINSSGKGKIRGQSGKKRGRPRK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHCCCCCCEEECCCCCCCCCCCC
YAHDPDIGEGVNVTEEDRKIFRVSISKFYNNPKENYLTTAYELMVKEYYKEGIRYDENGV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QKSILTPTDKIPTITQFKYWYQLEQDDVTKTLTSRKGAKRVALENRAILGNSKMETIGPG
HHHCCCCCCCCCCHHEEHEEEEECHHHHHHHHHHCCCCHHHHHCCCEEECCCCCCCCCCC
SRYQIDATVADVYLVSKYNRDWIIGRPVIYVIIDVFSRMITGVYVGLEGPSWIGAMMALA
CCEEEEHHHHHEEEEEECCCCEEECCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHH
NVATDKTLFCQEYNINITENEWPCNQMPDAILGDRGELAGMTVETLIPNLYVRIENAASY
HHCCCCEEEEEEECCEEECCCCCCCCCCHHHCCCCCCCCCCCHHHHCCCEEEEEECCCHH
RADWKGLVERHFRIIHEYVKPFLPGYVDTDFRQRGARDYRLDGKLDIDQFTEIIIRLIVH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEECCCCCCHHHHHHHHHHHHHH
HNNKPLTEYERDEAMIADDTPPIPRELWKWGIANRSGRLRTFSEDIVKLNLMPTEKATIT
CCCCCCCHHCCCCCEECCCCCCCCHHHHHHCCCCCCCCCEECCCCCEEEEEECCCCCEEE
AKGIKMRGKEMYYTCDSAKNEQWFEKARSNLLSKSEKSLIVSYDIRKPNFIYLPSSDGRD
ECCCEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCCCC
FEKCFLIDLEGRYANKNFHDIEYLLAYEELQRQKSQGKQLQEKVDLIADIESIVDRAEKM
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDASQYHNLSKSKKVSGIRDNRAFEKAKRRENEGFELAITDNQSSFQVAESTAETSEQPE
HHHHHHHCCCHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCC
SLQPNHLNLLKKKRQERKRGQN
CCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2156235; 1655576; 1657979; 8947057; 10704304 [H]