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The map label for this gene is tnsA [H]

Identifier: 75909692

GI number: 75909692

Start: 4324161

End: 4324979

Strand: Direct

Name: tnsA [H]

Synonym: Ava_3486

Alternate gene names: 75909692

Gene position: 4324161-4324979 (Clockwise)

Preceding gene: 75909691

Following gene: 75909693

Centisome position: 67.93

GC content: 36.87

Gene sequence:

>819_bases
ATGGTTAGGGGTAGAAGGGAATGGACTCAAGCTAAATATGAGCGATATATAAAAGAAGGTCGTGGCCGAGGAAGTGGTAA
GCACTATAAACCTTGGCTTACAATCCAAGATGTTCCCTCTAAAGGACGTTCTTCTAGAACTCCTGGTTGGAAAACAAATC
GAGTACATCATTTCTTCTCAGACCATGAAAAACGGCTATTTTATTTGTTTGAGTGGTCAGATATAGTCACAGATATAAGA
GAGCAATTTCCTTTAGACAATCTCGATCTAGCCATGAGTATTGCTACAGACATAGGTATAAAGTACCCTGTAGATACACA
AAGTGGCACTCCTCACGTTTTAACAAGCGACTTTATGCTTACAGTCAACCGCGATGGGAAGCAGATGCAGATTGCCCGGA
CTGTTAAGCCGTCAACAGAACTGGAGAAAAAGCAAGTTAATGAGAAATTCGAGATAGAAAGACAATATTACTTAGCAAAG
GGTATTGACTGGGGAATTATCACAGAAAAAGAAATTCCCAGGCTTTTAGCTGAAAATGTTGAGTGGGTTCATTCTGCTTA
TAGGCTAGAAGCAAATTCTGAAATGGACACTGCACAACTGAGTGAATTGTCAACAATTTTAAAATCTAAGCTTCAGGAAG
AAGATACAACTATAAATAGAGTAACTACTGCTTTAGACAAAGAAATGAATATAAATTCCGGTACATCACTTTATGTATTT
AAACATTTAATTGCTAGGAAAGAAATCCTTGTGGATCTAGTAGCAATAAAATTATCTGCTTGTCCGTCAACTAAAGTTAT
TGAAAAAATAATATTTTAG

Upstream 100 bases:

>100_bases
TATAATTCGGTTCAACAATTAATAAAACGATTGAACAATAACCTCTAAAGGAGTACGATTGTACCAAGTAAAAGTGCGAT
CGCTTCCAGAGGTTATTTTT

Downstream 100 bases:

>100_bases
ACCCACATGATAGGTAAATAATATGGTTAAAGATTTATTTGTCAATGATTTAATAGAGTGGATTGTATACCAAAACACTC
ATATAGTCGAAAGAATTATT

Product: Tn7-like protein transposition protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MVRGRREWTQAKYERYIKEGRGRGSGKHYKPWLTIQDVPSKGRSSRTPGWKTNRVHHFFSDHEKRLFYLFEWSDIVTDIR
EQFPLDNLDLAMSIATDIGIKYPVDTQSGTPHVLTSDFMLTVNRDGKQMQIARTVKPSTELEKKQVNEKFEIERQYYLAK
GIDWGIITEKEIPRLLAENVEWVHSAYRLEANSEMDTAQLSELSTILKSKLQEEDTTINRVTTALDKEMNINSGTSLYVF
KHLIARKEILVDLVAIKLSACPSTKVIEKIIF

Sequences:

>Translated_272_residues
MVRGRREWTQAKYERYIKEGRGRGSGKHYKPWLTIQDVPSKGRSSRTPGWKTNRVHHFFSDHEKRLFYLFEWSDIVTDIR
EQFPLDNLDLAMSIATDIGIKYPVDTQSGTPHVLTSDFMLTVNRDGKQMQIARTVKPSTELEKKQVNEKFEIERQYYLAK
GIDWGIITEKEIPRLLAENVEWVHSAYRLEANSEMDTAQLSELSTILKSKLQEEDTTINRVTTALDKEMNINSGTSLYVF
KHLIARKEILVDLVAIKLSACPSTKVIEKIIF
>Mature_272_residues
MVRGRREWTQAKYERYIKEGRGRGSGKHYKPWLTIQDVPSKGRSSRTPGWKTNRVHHFFSDHEKRLFYLFEWSDIVTDIR
EQFPLDNLDLAMSIATDIGIKYPVDTQSGTPHVLTSDFMLTVNRDGKQMQIARTVKPSTELEKKQVNEKFEIERQYYLAK
GIDWGIITEKEIPRLLAENVEWVHSAYRLEANSEMDTAQLSELSTILKSKLQEEDTTINRVTTALDKEMNINSGTSLYVF
KHLIARKEILVDLVAIKLSACPSTKVIEKIIF

Specific function: Required for Tn7 transposition. Forms the transposase, together with TnsB. TnsA executes the 5'-DNA strand breakage reaction. TnsABC and tnsD promote high-frequency insertion of Tn7 into a specific target site known as ATT-Tn7 whereas tnsABC and tnsD prom

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011856
- InterPro:   IPR011335
- InterPro:   IPR014832
- InterPro:   IPR014833
- InterPro:   IPR011991 [H]

Pfam domain/function: PF08721 TnsA_C; PF08722 TnsA_N [H]

EC number: NA

Molecular weight: Translated: 31538; Mature: 31538

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRGRREWTQAKYERYIKEGRGRGSGKHYKPWLTIQDVPSKGRSSRTPGWKTNRVHHFFS
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHC
DHEKRLFYLFEWSDIVTDIREQFPLDNLDLAMSIATDIGIKYPVDTQSGTPHVLTSDFML
CCCCEEEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEECCEEE
TVNRDGKQMQIARTVKPSTELEKKQVNEKFEIERQYYLAKGIDWGIITEKEIPRLLAENV
EECCCCCEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHH
EWVHSAYRLEANSEMDTAQLSELSTILKSKLQEEDTTINRVTTALDKEMNINSGTSLYVF
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
KHLIARKEILVDLVAIKLSACPSTKVIEKIIF
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC
>Mature Secondary Structure
MVRGRREWTQAKYERYIKEGRGRGSGKHYKPWLTIQDVPSKGRSSRTPGWKTNRVHHFFS
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHC
DHEKRLFYLFEWSDIVTDIREQFPLDNLDLAMSIATDIGIKYPVDTQSGTPHVLTSDFML
CCCCEEEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEECCEEE
TVNRDGKQMQIARTVKPSTELEKKQVNEKFEIERQYYLAKGIDWGIITEKEIPRLLAENV
EECCCCCEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHH
EWVHSAYRLEANSEMDTAQLSELSTILKSKLQEEDTTINRVTTALDKEMNINSGTSLYVF
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
KHLIARKEILVDLVAIKLSACPSTKVIEKIIF
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2156235; 1655576; 3010949; 8947057; 10704304 [H]