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The map label for this gene is rfbE [H]

Identifier: 75909558

GI number: 75909558

Start: 4172229

End: 4173296

Strand: Direct

Name: rfbE [H]

Synonym: Ava_3352

Alternate gene names: 75909558

Gene position: 4172229-4173296 (Clockwise)

Preceding gene: 75909557

Following gene: 75909559

Centisome position: 65.54

GC content: 36.99

Gene sequence:

>1068_bases
ATGGCTGAACGTGTTTTAATTTTAGGAGGGGCTGGTTTTATTGGCAGTTCTTTAGCAATCAGTCTCAAACAACAGCATCC
TTCATGGAAAATCAGTTGTTTTGATAATTTACGGAGACGAGGTTCAGAATTAAATCTGACTAGATTCAAAAAATCAGGAA
TTGAGTTTATTCACGGTGATATTCGCTCTTCTAGCGATTTAGAACCCGGAATGTTCAATATCGATACTATTATTGACTGT
TCTGCTGAACCTTCTGTTCTTGCAGGATTTTCCTCTCCACAATACGTCCTGCAAACAAATTTATTAGGAACTATTAATGT
TCTGGAACTAGCTAGACAAATCGATGCTCGTTTGCTTTTTCTCTCTACTAGTCGTGTTTATCCGATTGAAACACTGAAGT
CCATTAATCTGATAGAATCACCAACCCGATTGACTATTGCTCCTGAGCAAACAATTTCCGGAGTATCTAATCTTGGTATT
GCCGAAGACTTTCCTCTTCAAAGCTACCGTTCTCTATATGGTACTACTAAACTTGCATCAGAGATGCTAATTGAAGAGTA
TCGACAAGCTTACGGTATTCAAGCAATAGTTAATCGTTGTGGCGTAATTACAGGGCCTTGGCAAATGGGTAAAGTTGACC
AAGGTGTTTTTGTTTTATGGTTAGCTGCACACTATTTTGAGAAATCTTTAAGTTATATCGGCTACGGTGGTACAGGTAAA
CAAGTGAGAGATTTATTACATATTGAAGATTTATTAAGACTAATTAGCTACCAATTAGAACATTTTTCGGAACTAGGTGG
GGATGTATTAAATGTTGGTGGAGGTGCTGATAATAGTCTATCTCTATTAGAAACTACTAAGCTCTGTGAAGCAATTACGG
GTAAGTCTATTCCTATTAAATCAGAAGTCACAGCTAGACAGGGTGATATACCCATTTACATTACAGATTCTTCCAAAATT
ATCTCTAAGACTGGATGGAAACCAACCATGAATCCAGAACAAACTCTGCGAGATATTTATAGTTGGATTCTTGAATATGA
GGAATTTCTTCAGCCCATTTTATCCTAA

Upstream 100 bases:

>100_bases
TTGTTTATAGTTCTCTACATTTGGTTAGAAAAACACCTGTCCCGTGGAGATTACCATCTCAGTAAAAAAAGAACTGTCGT
TAAATCAAAGGCTGAATCGA

Downstream 100 bases:

>100_bases
CCTGTTTACTATACTTTGATGTGAGAAATAATATCATGCCCACTGTTCTAATTACTGGTTCATCTGGTCTGATAGGTTCA
GAATCCGTTCATTTTTTCGC

Product: 3-beta hydroxysteroid dehydrogenase/isomerase

Products: NA

Alternate protein names: CDP-tyvelose 2-epimerase [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MAERVLILGGAGFIGSSLAISLKQQHPSWKISCFDNLRRRGSELNLTRFKKSGIEFIHGDIRSSSDLEPGMFNIDTIIDC
SAEPSVLAGFSSPQYVLQTNLLGTINVLELARQIDARLLFLSTSRVYPIETLKSINLIESPTRLTIAPEQTISGVSNLGI
AEDFPLQSYRSLYGTTKLASEMLIEEYRQAYGIQAIVNRCGVITGPWQMGKVDQGVFVLWLAAHYFEKSLSYIGYGGTGK
QVRDLLHIEDLLRLISYQLEHFSELGGDVLNVGGGADNSLSLLETTKLCEAITGKSIPIKSEVTARQGDIPIYITDSSKI
ISKTGWKPTMNPEQTLRDIYSWILEYEEFLQPILS

Sequences:

>Translated_355_residues
MAERVLILGGAGFIGSSLAISLKQQHPSWKISCFDNLRRRGSELNLTRFKKSGIEFIHGDIRSSSDLEPGMFNIDTIIDC
SAEPSVLAGFSSPQYVLQTNLLGTINVLELARQIDARLLFLSTSRVYPIETLKSINLIESPTRLTIAPEQTISGVSNLGI
AEDFPLQSYRSLYGTTKLASEMLIEEYRQAYGIQAIVNRCGVITGPWQMGKVDQGVFVLWLAAHYFEKSLSYIGYGGTGK
QVRDLLHIEDLLRLISYQLEHFSELGGDVLNVGGGADNSLSLLETTKLCEAITGKSIPIKSEVTARQGDIPIYITDSSKI
ISKTGWKPTMNPEQTLRDIYSWILEYEEFLQPILS
>Mature_354_residues
AERVLILGGAGFIGSSLAISLKQQHPSWKISCFDNLRRRGSELNLTRFKKSGIEFIHGDIRSSSDLEPGMFNIDTIIDCS
AEPSVLAGFSSPQYVLQTNLLGTINVLELARQIDARLLFLSTSRVYPIETLKSINLIESPTRLTIAPEQTISGVSNLGIA
EDFPLQSYRSLYGTTKLASEMLIEEYRQAYGIQAIVNRCGVITGPWQMGKVDQGVFVLWLAAHYFEKSLSYIGYGGTGKQ
VRDLLHIEDLLRLISYQLEHFSELGGDVLNVGGGADNSLSLLETTKLCEAITGKSIPIKSEVTARQGDIPIYITDSSKII
SKTGWKPTMNPEQTLRDIYSWILEYEEFLQPILS

Specific function: Catalyzes the isomeration of CDP-paratose to CDP- tyvelose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=355, Percent_Identity=23.943661971831, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1788353, Length=374, Percent_Identity=25.1336898395722, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1786974, Length=359, Percent_Identity=24.2339832869081, Blast_Score=76, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356223, Length=251, Percent_Identity=30.2788844621514, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.10 [H]

Molecular weight: Translated: 39377; Mature: 39245

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERVLILGGAGFIGSSLAISLKQQHPSWKISCFDNLRRRGSELNLTRFKKSGIEFIHGD
CCCEEEEEECCCHHCCHHEEEEECCCCCEEEEHHHHHHHCCCCCCHHHHHHHCCCEEECC
IRSSSDLEPGMFNIDTIIDCSAEPSVLAGFSSPQYVLQTNLLGTINVLELARQIDARLLF
CCCCCCCCCCCCCCCEEEECCCCCCEEECCCCCCEEEECCCCHHHHHHHHHHHHHHEEEE
LSTSRVYPIETLKSINLIESPTRLTIAPEQTISGVSNLGIAEDFPLQSYRSLYGTTKLAS
EECCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
EMLIEEYRQAYGIQAIVNRCGVITGPWQMGKVDQGVFVLWLAAHYFEKSLSYIGYGGTGK
HHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCHH
QVRDLLHIEDLLRLISYQLEHFSELGGDVLNVGGGADNSLSLLETTKLCEAITGKSIPIK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCC
SEVTARQGDIPIYITDSSKIISKTGWKPTMNPEQTLRDIYSWILEYEEFLQPILS
CCCCCCCCCEEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AERVLILGGAGFIGSSLAISLKQQHPSWKISCFDNLRRRGSELNLTRFKKSGIEFIHGD
CCEEEEEECCCHHCCHHEEEEECCCCCEEEEHHHHHHHCCCCCCHHHHHHHCCCEEECC
IRSSSDLEPGMFNIDTIIDCSAEPSVLAGFSSPQYVLQTNLLGTINVLELARQIDARLLF
CCCCCCCCCCCCCCCEEEECCCCCCEEECCCCCCEEEECCCCHHHHHHHHHHHHHHEEEE
LSTSRVYPIETLKSINLIESPTRLTIAPEQTISGVSNLGIAEDFPLQSYRSLYGTTKLAS
EECCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
EMLIEEYRQAYGIQAIVNRCGVITGPWQMGKVDQGVFVLWLAAHYFEKSLSYIGYGGTGK
HHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCHH
QVRDLLHIEDLLRLISYQLEHFSELGGDVLNVGGGADNSLSLLETTKLCEAITGKSIPIK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCC
SEVTARQGDIPIYITDSSKIISKTGWKPTMNPEQTLRDIYSWILEYEEFLQPILS
CCCCCCCCCEEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2793833; 11677608; 12644504; 12642575 [H]