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The map label for this gene is rfbE [H]

Identifier: 75909559

GI number: 75909559

Start: 4173332

End: 4174414

Strand: Direct

Name: rfbE [H]

Synonym: Ava_3353

Alternate gene names: 75909559

Gene position: 4173332-4174414 (Clockwise)

Preceding gene: 75909558

Following gene: 75909560

Centisome position: 65.56

GC content: 39.89

Gene sequence:

>1083_bases
ATGCCCACTGTTCTAATTACTGGTTCATCTGGTCTGATAGGTTCAGAATCCGTTCATTTTTTCGCCCAGAAAGGTTTTCG
GATTTTGGGCATTGATAATAATATGCGTGCGGTGTTTTTTGGTGAATCTGCTTCGACAGACTGGAATCGTCAAATTTTAG
AAGATAAATATGGTGAGAGGTATCGCCACTATGATATCGATATCCGCGATCGCACTGCTATAGAAAACCTCTTTCAAGAA
TATTCTACTGATATTTCTCTCATTATCCATACAGCAGCGCAACCATCCCATGATTGGGCTGCAAAAGACCCCCACACAGA
TTTTACAGTCAATGCCAACGGCACTTTAGTTCTATTAGAAGCAACTCGACAATACTGTCCGCAAGCAGTTTTTATATTTA
CTTCCACTAACAAAGTCTATGGCGATACCCCTAACTATCTACCTTTACAGGAGTTAGAGACTCGCTGGGAAATTGCGCCC
AATCATCAATACAAAGAAGGTATTGATGAGTCAATGACAATTGACAACTCTAAGCATTCCCTCTTTGGGGCTTCCAAAGT
TGCCGCAGATGTACTAGTTCAAGAATATGGCAGATATTTCGGCATGAAAACTGCCAGTTTTAGAGGTGGTTGTTTGACTG
GGCCTAATCATTCTGGCGCTCAATTGCATGGCTTTCTCGCTTACTTAATGAAATGTACTATTTCGGGTGATACGTACACT
ATCTTTGGCTACAAAGGCAAACAGGTTAGAGATAATATCCACAGTTACGACTTAGTGAACGCCTTTTATCACTTCTATCA
AAACCCCAGATGTGGAGAGATTTATAACATCGGTGGTTCTCGGCATAGTAATTGTTCTATGTTAGAAGCGATCGCAATTT
GCGAAAATATCGTCGGTAAAAAGCTGCAATATACCTATACAGATGATAATCGATCTGGCGATCACATCTGGTATATTTCA
GATGTGGGTAAGTTTCGCTCTCACTATCCCACATGGGAGTATAAATATAATCTTCAACAAATACTAGAGGAGATTTATTA
TGCTCAAACATCTAGACAAACTAATTACGCGAAAAGTCATTAA

Upstream 100 bases:

>100_bases
CTCTGCGAGATATTTATAGTTGGATTCTTGAATATGAGGAATTTCTTCAGCCCATTTTATCCTAACCTGTTTACTATACT
TTGATGTGAGAAATAATATC

Downstream 100 bases:

>100_bases
GTAATCCTGCCAAAATACCAGTTAAGTTAAAATCTGTGAAAGTTGTTACCACTGAAATCTCTGATATTTTACTCCTCGAA
CCGCAAGTATTTACTGACTC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: CDP-tyvelose 2-epimerase [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MPTVLITGSSGLIGSESVHFFAQKGFRILGIDNNMRAVFFGESASTDWNRQILEDKYGERYRHYDIDIRDRTAIENLFQE
YSTDISLIIHTAAQPSHDWAAKDPHTDFTVNANGTLVLLEATRQYCPQAVFIFTSTNKVYGDTPNYLPLQELETRWEIAP
NHQYKEGIDESMTIDNSKHSLFGASKVAADVLVQEYGRYFGMKTASFRGGCLTGPNHSGAQLHGFLAYLMKCTISGDTYT
IFGYKGKQVRDNIHSYDLVNAFYHFYQNPRCGEIYNIGGSRHSNCSMLEAIAICENIVGKKLQYTYTDDNRSGDHIWYIS
DVGKFRSHYPTWEYKYNLQQILEEIYYAQTSRQTNYAKSH

Sequences:

>Translated_360_residues
MPTVLITGSSGLIGSESVHFFAQKGFRILGIDNNMRAVFFGESASTDWNRQILEDKYGERYRHYDIDIRDRTAIENLFQE
YSTDISLIIHTAAQPSHDWAAKDPHTDFTVNANGTLVLLEATRQYCPQAVFIFTSTNKVYGDTPNYLPLQELETRWEIAP
NHQYKEGIDESMTIDNSKHSLFGASKVAADVLVQEYGRYFGMKTASFRGGCLTGPNHSGAQLHGFLAYLMKCTISGDTYT
IFGYKGKQVRDNIHSYDLVNAFYHFYQNPRCGEIYNIGGSRHSNCSMLEAIAICENIVGKKLQYTYTDDNRSGDHIWYIS
DVGKFRSHYPTWEYKYNLQQILEEIYYAQTSRQTNYAKSH
>Mature_359_residues
PTVLITGSSGLIGSESVHFFAQKGFRILGIDNNMRAVFFGESASTDWNRQILEDKYGERYRHYDIDIRDRTAIENLFQEY
STDISLIIHTAAQPSHDWAAKDPHTDFTVNANGTLVLLEATRQYCPQAVFIFTSTNKVYGDTPNYLPLQELETRWEIAPN
HQYKEGIDESMTIDNSKHSLFGASKVAADVLVQEYGRYFGMKTASFRGGCLTGPNHSGAQLHGFLAYLMKCTISGDTYTI
FGYKGKQVRDNIHSYDLVNAFYHFYQNPRCGEIYNIGGSRHSNCSMLEAIAICENIVGKKLQYTYTDDNRSGDHIWYISD
VGKFRSHYPTWEYKYNLQQILEEIYYAQTSRQTNYAKSH

Specific function: Catalyzes the isomeration of CDP-paratose to CDP- tyvelose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI56237023, Length=321, Percent_Identity=29.2834890965732, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI56118217, Length=321, Percent_Identity=29.2834890965732, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI189083684, Length=321, Percent_Identity=29.2834890965732, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1788353, Length=373, Percent_Identity=26.5415549597855, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI48994969, Length=305, Percent_Identity=27.5409836065574, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786974, Length=365, Percent_Identity=24.9315068493151, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71982038, Length=301, Percent_Identity=27.5747508305648, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=264, Percent_Identity=28.7878787878788, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19923002, Length=368, Percent_Identity=26.6304347826087, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.10 [H]

Molecular weight: Translated: 41177; Mature: 41046

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTVLITGSSGLIGSESVHFFAQKGFRILGIDNNMRAVFFGESASTDWNRQILEDKYGER
CCEEEEECCCCCCCCCCHHHHHHCCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHHCCC
YRHYDIDIRDRTAIENLFQEYSTDISLIIHTAAQPSHDWAAKDPHTDFTVNANGTLVLLE
CEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEEE
ATRQYCPQAVFIFTSTNKVYGDTPNYLPLQELETRWEIAPNHQYKEGIDESMTIDNSKHS
CHHHHCCCEEEEEEECCEEECCCCCCCCHHHHHHHCCCCCCCCHHHCCCCCEEECCCCHH
LFGASKVAADVLVQEYGRYFGMKTASFRGGCLTGPNHSGAQLHGFLAYLMKCTISGDTYT
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCEEE
IFGYKGKQVRDNIHSYDLVNAFYHFYQNPRCGEIYNIGGSRHSNCSMLEAIAICENIVGK
EEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCC
KLQYTYTDDNRSGDHIWYISDVGKFRSHYPTWEYKYNLQQILEEIYYAQTSRQTNYAKSH
CEEEEECCCCCCCCEEEEEECHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PTVLITGSSGLIGSESVHFFAQKGFRILGIDNNMRAVFFGESASTDWNRQILEDKYGER
CEEEEECCCCCCCCCCHHHHHHCCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHHCCC
YRHYDIDIRDRTAIENLFQEYSTDISLIIHTAAQPSHDWAAKDPHTDFTVNANGTLVLLE
CEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEEE
ATRQYCPQAVFIFTSTNKVYGDTPNYLPLQELETRWEIAPNHQYKEGIDESMTIDNSKHS
CHHHHCCCEEEEEEECCEEECCCCCCCCHHHHHHHCCCCCCCCHHHCCCCCEEECCCCHH
LFGASKVAADVLVQEYGRYFGMKTASFRGGCLTGPNHSGAQLHGFLAYLMKCTISGDTYT
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCEEE
IFGYKGKQVRDNIHSYDLVNAFYHFYQNPRCGEIYNIGGSRHSNCSMLEAIAICENIVGK
EEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCC
KLQYTYTDDNRSGDHIWYISDVGKFRSHYPTWEYKYNLQQILEEIYYAQTSRQTNYAKSH
CEEEEECCCCCCCCEEEEEECHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2793833; 11677608; 12644504; 12642575 [H]