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The map label for this gene is dapF [H]

Identifier: 75909296

GI number: 75909296

Start: 3838451

End: 3839299

Strand: Direct

Name: dapF [H]

Synonym: Ava_3088

Alternate gene names: 75909296

Gene position: 3838451-3839299 (Clockwise)

Preceding gene: 75909294

Following gene: 75909297

Centisome position: 60.3

GC content: 45.58

Gene sequence:

>849_bases
ATGGGAAAAATGGCAATCGAATTTACTAAGTATCACGGTCTAGGCAACGATTTTATTCTCATCGATAATCGCGCTTCTAA
AACGCCGGCAATCACTCCAGAGAAAGCGGTGGAGATGTGCGATCGCCACTTTGGTATCGGTGCTGATGGTGTAATTTTTG
CCTTACCTGGAGAAAACGGTACTGATTACACCATGCGGATTTTTAATTCCGATGGTTCAGAACCGGAAATGTGTGGTAAT
GGTATTCGCTGTCTCGCTGCATTTCTGGCTGATTTGGAAGGGCTTTCTCGTAATAAGGATACCTATCGTATTCATACTTT
AGCTGGTGTGATTACCCCCCAACTCACCCCTGATGGACAAATCAAGGTGGATATGGGTTTACCTCGGTTACTAGCTGGGG
AAATTCCGACAAATATTGCTGCTGCTGATCAGAAAGTAATTAACCAACCCCTAGAAGTAGAAGGGAAAACTTGGGAAGTT
ACTTGTGTCAGCATGGGGAATCCCCACTGTATTACTTTCGTGGAAGACGTGGCAGCGATTCCTTTAGAAACCATCGGCCC
TAAATTTGAACATCACCCAGCTTTTCCTCAACGGACAAACACCGAATTTATTCAAGTTGTCAGCCGTGATTACTTGAAGA
TGCGTGTCTGGGAACGCGGTGCAGGAATTACTTTAGCTTGTGGTACAGGTGCTTGTGCTTCCTTGGTAGCGGCGGTATTG
ACTGGAAGAAGCGATCGCCTCGCTACTGTAGAATTGCCTGGTGGCCCTTTGGAAATTGAATGGTCAGAAGTAGACCAAAG
AATTTATATGACCGGCCCAGCTGATAGGGTATTCACAGGTAAACTGTGA

Upstream 100 bases:

>100_bases
AGCCATTTTTGATAATGATGGAATAATTGAGGGATTAGGTAATGGGTGATGAGTAATGGGTAATTACCAATCCTCAATAC
CTAATCATTGATAATTCATC

Downstream 100 bases:

>100_bases
GCAAAAATCCTGGGTGCTGAGTTGAAAGACCACTCATAAATGTTTTACACCCCTACACCCGGTCTCAACAGAAAATCTGG
GTGCGTCAGTCCTAACTCAT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase 2 [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MGKMAIEFTKYHGLGNDFILIDNRASKTPAITPEKAVEMCDRHFGIGADGVIFALPGENGTDYTMRIFNSDGSEPEMCGN
GIRCLAAFLADLEGLSRNKDTYRIHTLAGVITPQLTPDGQIKVDMGLPRLLAGEIPTNIAAADQKVINQPLEVEGKTWEV
TCVSMGNPHCITFVEDVAAIPLETIGPKFEHHPAFPQRTNTEFIQVVSRDYLKMRVWERGAGITLACGTGACASLVAAVL
TGRSDRLATVELPGGPLEIEWSEVDQRIYMTGPADRVFTGKL

Sequences:

>Translated_282_residues
MGKMAIEFTKYHGLGNDFILIDNRASKTPAITPEKAVEMCDRHFGIGADGVIFALPGENGTDYTMRIFNSDGSEPEMCGN
GIRCLAAFLADLEGLSRNKDTYRIHTLAGVITPQLTPDGQIKVDMGLPRLLAGEIPTNIAAADQKVINQPLEVEGKTWEV
TCVSMGNPHCITFVEDVAAIPLETIGPKFEHHPAFPQRTNTEFIQVVSRDYLKMRVWERGAGITLACGTGACASLVAAVL
TGRSDRLATVELPGGPLEIEWSEVDQRIYMTGPADRVFTGKL
>Mature_281_residues
GKMAIEFTKYHGLGNDFILIDNRASKTPAITPEKAVEMCDRHFGIGADGVIFALPGENGTDYTMRIFNSDGSEPEMCGNG
IRCLAAFLADLEGLSRNKDTYRIHTLAGVITPQLTPDGQIKVDMGLPRLLAGEIPTNIAAADQKVINQPLEVEGKTWEVT
CVSMGNPHCITFVEDVAAIPLETIGPKFEHHPAFPQRTNTEFIQVVSRDYLKMRVWERGAGITLACGTGACASLVAAVLT
GRSDRLATVELPGGPLEIEWSEVDQRIYMTGPADRVFTGKL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=276, Percent_Identity=41.304347826087, Blast_Score=213, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30635; Mature: 30504

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKMAIEFTKYHGLGNDFILIDNRASKTPAITPEKAVEMCDRHFGIGADGVIFALPGENG
CCCEEEEEEEECCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCC
TDYTMRIFNSDGSEPEMCGNGIRCLAAFLADLEGLSRNKDTYRIHTLAGVITPQLTPDGQ
CCEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCC
IKVDMGLPRLLAGEIPTNIAAADQKVINQPLEVEGKTWEVTCVSMGNPHCITFVEDVAAI
EEECCCCCHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEEEHHHHHC
PLETIGPKFEHHPAFPQRTNTEFIQVVSRDYLKMRVWERGAGITLACGTGACASLVAAVL
CHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCEEEEECCCHHHHHHHHHH
TGRSDRLATVELPGGPLEIEWSEVDQRIYMTGPADRVFTGKL
CCCCCCEEEEECCCCCEEEEHHHCCCEEEEECCCCCEEECCC
>Mature Secondary Structure 
GKMAIEFTKYHGLGNDFILIDNRASKTPAITPEKAVEMCDRHFGIGADGVIFALPGENG
CCEEEEEEEECCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCC
TDYTMRIFNSDGSEPEMCGNGIRCLAAFLADLEGLSRNKDTYRIHTLAGVITPQLTPDGQ
CCEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCC
IKVDMGLPRLLAGEIPTNIAAADQKVINQPLEVEGKTWEVTCVSMGNPHCITFVEDVAAI
EEECCCCCHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEEEHHHHHC
PLETIGPKFEHHPAFPQRTNTEFIQVVSRDYLKMRVWERGAGITLACGTGACASLVAAVL
CHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCEEEEECCCHHHHHHHHHH
TGRSDRLATVELPGGPLEIEWSEVDQRIYMTGPADRVFTGKL
CCCCCCEEEEECCCCCEEEEHHHCCCEEEEECCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]