Click here to switch to the map view.

The map label for this gene is cobS

Identifier: 75909036

GI number: 75909036

Start: 3495846

End: 3496604

Strand: Direct

Name: cobS

Synonym: Ava_2824

Alternate gene names: 75909036

Gene position: 3495846-3496604 (Clockwise)

Preceding gene: 75909034

Following gene: 75909039

Centisome position: 54.92

GC content: 43.61

Gene sequence:

>759_bases
ATGGTGAAGCTGTTGTTATTGAATTTATTGGCAAGTATTATTTTTTATACAAGTATTCCTCTGCCTTATATCAAAGGTTT
AGATTTTCAAAAGGTGGCGCGGCTTGTTCCTATGGTGGGGTTAATCATTGGGGTAATTTTAGGGTTACTTGATGGAGGTA
TGAATTATTTGGGAATGCCCGTATTAACTCGTAGTGCCTTGGTAGTGGCATTGTGGATTTTTATCACTGGGGGGTTACAT
TTAGATGGGGCGATGGATACGGCTGATGGTTTGGCTGTGGGTGACCCAGAACGGCGTTTGCAAGTGATGGCGGATAGTGC
TACAGGTGCATTTGGAGCTATGAGTGCGATCGCTATATTATTACTAAAAACCTCTGCCCTCACAGAGATTGGGGAATACC
GTTGGCTAGTATTAATGGCTGCTTGTGGCTGGGGACGTTGGGGACAACAAGTAGCGATCGCCTGTTATCCTTATTTAAAA
GCCACTGGTAAAGGAGCATTCCATAAACAAGCTATTCGCTCATACAAAGACTTATTACCCGGATTATGCTTGATGGTTGC
AGTCAGTAGCTTATTTTGGCTAGTAAATAACCATCATCTATTAATAACAGTAGTCGGATTAATTACTGGGAGTGCGATCG
CTAGTTTAACAGCTGCATGGTTTAATCACAAATTAGGCGGACACACCGGCGACACCTACGGCGCAGTCGTCGAGTGGACA
GAGGCTCTATTTCTCTGCGTACTGACCATACTCACATAA

Upstream 100 bases:

>100_bases
TTGAGAATGTTGGGTTCCGTTCCTCCACCCAACCTACACCAGTCTAAGTTGTTGTCTTAACTGTACTGTATTGGGTTATG
GGTGATGAAGTGAGATTGAA

Downstream 100 bases:

>100_bases
TGTACCATGTAGGCGATCGTTCTATCTGTGTTTATCTGCGTGCATCTGCGTTTAATTTTATTCCAGTTTATCCCATCATT
TTAGCCTTGCCACGCCACTA

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MVKLLLLNLLASIIFYTSIPLPYIKGLDFQKVARLVPMVGLIIGVILGLLDGGMNYLGMPVLTRSALVVALWIFITGGLH
LDGAMDTADGLAVGDPERRLQVMADSATGAFGAMSAIAILLLKTSALTEIGEYRWLVLMAACGWGRWGQQVAIACYPYLK
ATGKGAFHKQAIRSYKDLLPGLCLMVAVSSLFWLVNNHHLLITVVGLITGSAIASLTAAWFNHKLGGHTGDTYGAVVEWT
EALFLCVLTILT

Sequences:

>Translated_252_residues
MVKLLLLNLLASIIFYTSIPLPYIKGLDFQKVARLVPMVGLIIGVILGLLDGGMNYLGMPVLTRSALVVALWIFITGGLH
LDGAMDTADGLAVGDPERRLQVMADSATGAFGAMSAIAILLLKTSALTEIGEYRWLVLMAACGWGRWGQQVAIACYPYLK
ATGKGAFHKQAIRSYKDLLPGLCLMVAVSSLFWLVNNHHLLITVVGLITGSAIASLTAAWFNHKLGGHTGDTYGAVVEWT
EALFLCVLTILT
>Mature_252_residues
MVKLLLLNLLASIIFYTSIPLPYIKGLDFQKVARLVPMVGLIIGVILGLLDGGMNYLGMPVLTRSALVVALWIFITGGLH
LDGAMDTADGLAVGDPERRLQVMADSATGAFGAMSAIAILLLKTSALTEIGEYRWLVLMAACGWGRWGQQVAIACYPYLK
ATGKGAFHKQAIRSYKDLLPGLCLMVAVSSLFWLVNNHHLLITVVGLITGSAIASLTAAWFNHKLGGHTGDTYGAVVEWT
EALFLCVLTILT

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=251, Percent_Identity=29.8804780876494, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_ANAVT (Q3M999)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_323332.1
- STRING:   Q3M999
- GeneID:   3681524
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_2824
- NMPDR:   fig|240292.3.peg.1534
- eggNOG:   COG0368
- HOGENOM:   HBG729392
- OMA:   GWGRWGQ
- PhylomeDB:   Q3M999
- ProtClustDB:   PRK00235
- BioCyc:   AVAR240292:AVA_2824-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 27032; Mature: 27032

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd232d94)-; HASH(0x1194fd4c)-; HASH(0x11973fac)-; HASH(0x113a1ffc)-; HASH(0xfc2fd44)-; HASH(0xe2daa74)-; HASH(0x112cf4d8)-;

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLLLLNLLASIIFYTSIPLPYIKGLDFQKVARLVPMVGLIIGVILGLLDGGMNYLGMP
CHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHH
VLTRSALVVALWIFITGGLHLDGAMDTADGLAVGDPERRLQVMADSATGAFGAMSAIAIL
HHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
LLKTSALTEIGEYRWLVLMAACGWGRWGQQVAIACYPYLKATGKGAFHKQAIRSYKDLLP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GLCLMVAVSSLFWLVNNHHLLITVVGLITGSAIASLTAAWFNHKLGGHTGDTYGAVVEWT
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
EALFLCVLTILT
HHHHHHHHHHHH
>Mature Secondary Structure
MVKLLLLNLLASIIFYTSIPLPYIKGLDFQKVARLVPMVGLIIGVILGLLDGGMNYLGMP
CHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHH
VLTRSALVVALWIFITGGLHLDGAMDTADGLAVGDPERRLQVMADSATGAFGAMSAIAIL
HHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
LLKTSALTEIGEYRWLVLMAACGWGRWGQQVAIACYPYLKATGKGAFHKQAIRSYKDLLP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GLCLMVAVSSLFWLVNNHHLLITVVGLITGSAIASLTAAWFNHKLGGHTGDTYGAVVEWT
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
EALFLCVLTILT
HHHHHHHHHHHH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA