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The map label for this gene is pleD [H]

Identifier: 75908369

GI number: 75908369

Start: 2660863

End: 2662194

Strand: Direct

Name: pleD [H]

Synonym: Ava_2150

Alternate gene names: 75908369

Gene position: 2660863-2662194 (Clockwise)

Preceding gene: 75908368

Following gene: 75908370

Centisome position: 41.8

GC content: 35.29

Gene sequence:

>1332_bases
ATGCTTGATACATCAACCGAAACTAGCTTTATTTTAGTTGTAGATGATACCCTCACCAATCTCGAAATTATCTCTGAAGC
ATTGATTGGCGCAGGTTTTGAAGTAGCCACCGCAGTTGATGGAAAAAAAGCGCTCGAACAGATACAAAGTAGAGTGCCAG
ATTTAATTCTGTTGGATGTAATGATGCCGGTAATGGACGGATTTGAAACCTGTAAAAACTTGAAAATTCAGCCAGAAACT
CACGATATTCCCGTGATTTTCATGACAGCAATTACTGATACAAATAGTAAAGTAGAAGCCTTAAGTTTAGGCGCAGTTGA
TTACATTACTAAGCCATTCCATAAAGCAGAAGTTTTGGCTCGTATTTCTGCCCATCTGCAACTGAGAAGCTTGAATAAAA
ATTTAGAAAAACGAGTTATTGAACGCACGGCTGAATTGAATCAAGCATTGAAAGACCTACAAGAGTATCAACTCCAACTT
GTACAACAGGAAAAAATGTCTGTGCTTGGACAATTAGTAGCAGGATTAGCTCATGAAATTAATAACCCAGTCAGTTGTAT
TTATGGCAATTTAGGTCACGCTTTGACGTATTTTGAAAATATGGATAAGCTGATTAATTTATATCAAAGTTATTATCCCC
AGCCTGTACAAGAAATTCAAAACGAAATCAGTGAAATGGATTTAGCCTATGTGCGTTCTGACCTGCCCAATTTAATTTTC
TCTATGAAAGAAGGCATTCAGCGCATCCGAGATATTAGCAGGAGTCTACGTATTTTTTCCAGGTCAGACACAGAAAAAAA
AATTCAGTTTGATATTCATGAAGGGATTGATAGTACTATTTTAATACTCAAGCATCGATTGAAAGGATCTGAGAATCACC
CTGACATTGAAATTAAACGAGATTATGGTGTCATACCTCAAGTTAACTGCTTTCCTGGGCAATTGAATCAGGTGTTCATG
AATATTTTAGCTAATGCAATTGATGCCTTAGAAGAATCATTTAGTAATAATTATTATTCAGTAATAGATAGTGAAATTAC
TGTTAATCAGGAAATGATGGCTGAGACTCCGCAGATCATAATTCAGACAATACTGAGTGAAGATGAAAGTTATGCTTTAA
TTAAAATTAAAGATAACGGCATAGGAATGCCACAGCAAATTAAAGAGAGAATTTTTGATAATTTGTTTACTACTAAACCT
GTAGGTAAAGGTACAGGTTTAGGATTATCAATTGCTCGGCAAATTATCATTCAAAAACATGGTGGTAAACTTGAGGTAAA
TTCAGAACCAGGAAGAGGCTCAGAATTTATTATTAACATCCCCATTGAATAA

Upstream 100 bases:

>100_bases
AAGAATAATTTAAGCGATTAACACACCCAAGGTTTACTTTATAAATAGTCTCCTATCGCATCACCAGTGAATTAATATAA
AATCCGCGAGTTCAAATCAA

Downstream 100 bases:

>100_bases
CTATATCAATTGTCAAACTTAATTTCTCTAGCTAATTATGTATTTTGCTAGAACTTAATTAAAGCCAGGATGTTTTCTAT
GATTCTTCAATGATTGATAA

Product: response regulator receiver signal transduction histidine kinase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MLDTSTETSFILVVDDTLTNLEIISEALIGAGFEVATAVDGKKALEQIQSRVPDLILLDVMMPVMDGFETCKNLKIQPET
HDIPVIFMTAITDTNSKVEALSLGAVDYITKPFHKAEVLARISAHLQLRSLNKNLEKRVIERTAELNQALKDLQEYQLQL
VQQEKMSVLGQLVAGLAHEINNPVSCIYGNLGHALTYFENMDKLINLYQSYYPQPVQEIQNEISEMDLAYVRSDLPNLIF
SMKEGIQRIRDISRSLRIFSRSDTEKKIQFDIHEGIDSTILILKHRLKGSENHPDIEIKRDYGVIPQVNCFPGQLNQVFM
NILANAIDALEESFSNNYYSVIDSEITVNQEMMAETPQIIIQTILSEDESYALIKIKDNGIGMPQQIKERIFDNLFTTKP
VGKGTGLGLSIARQIIIQKHGGKLEVNSEPGRGSEFIINIPIE

Sequences:

>Translated_443_residues
MLDTSTETSFILVVDDTLTNLEIISEALIGAGFEVATAVDGKKALEQIQSRVPDLILLDVMMPVMDGFETCKNLKIQPET
HDIPVIFMTAITDTNSKVEALSLGAVDYITKPFHKAEVLARISAHLQLRSLNKNLEKRVIERTAELNQALKDLQEYQLQL
VQQEKMSVLGQLVAGLAHEINNPVSCIYGNLGHALTYFENMDKLINLYQSYYPQPVQEIQNEISEMDLAYVRSDLPNLIF
SMKEGIQRIRDISRSLRIFSRSDTEKKIQFDIHEGIDSTILILKHRLKGSENHPDIEIKRDYGVIPQVNCFPGQLNQVFM
NILANAIDALEESFSNNYYSVIDSEITVNQEMMAETPQIIIQTILSEDESYALIKIKDNGIGMPQQIKERIFDNLFTTKP
VGKGTGLGLSIARQIIIQKHGGKLEVNSEPGRGSEFIINIPIE
>Mature_443_residues
MLDTSTETSFILVVDDTLTNLEIISEALIGAGFEVATAVDGKKALEQIQSRVPDLILLDVMMPVMDGFETCKNLKIQPET
HDIPVIFMTAITDTNSKVEALSLGAVDYITKPFHKAEVLARISAHLQLRSLNKNLEKRVIERTAELNQALKDLQEYQLQL
VQQEKMSVLGQLVAGLAHEINNPVSCIYGNLGHALTYFENMDKLINLYQSYYPQPVQEIQNEISEMDLAYVRSDLPNLIF
SMKEGIQRIRDISRSLRIFSRSDTEKKIQFDIHEGIDSTILILKHRLKGSENHPDIEIKRDYGVIPQVNCFPGQLNQVFM
NILANAIDALEESFSNNYYSVIDSEITVNQEMMAETPQIIIQTILSEDESYALIKIKDNGIGMPQQIKERIFDNLFTTKP
VGKGTGLGLSIARQIIIQKHGGKLEVNSEPGRGSEFIINIPIE

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=289, Percent_Identity=28.719723183391, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790437, Length=104, Percent_Identity=46.1538461538462, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1786599, Length=114, Percent_Identity=42.9824561403509, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788550, Length=151, Percent_Identity=35.7615894039735, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790299, Length=134, Percent_Identity=35.8208955223881, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790436, Length=300, Percent_Identity=26, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082012, Length=127, Percent_Identity=36.2204724409449, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1789809, Length=117, Percent_Identity=34.1880341880342, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788394, Length=130, Percent_Identity=30.7692307692308, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786911, Length=120, Percent_Identity=34.1666666666667, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790860, Length=97, Percent_Identity=37.1134020618557, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787375, Length=132, Percent_Identity=34.0909090909091, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790300, Length=303, Percent_Identity=27.0627062706271, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI2367329, Length=117, Percent_Identity=43.5897435897436, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789402, Length=117, Percent_Identity=32.4786324786325, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 49834; Mature: 49834

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDTSTETSFILVVDDTLTNLEIISEALIGAGFEVATAVDGKKALEQIQSRVPDLILLDV
CCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHCCHHHHHHH
MMPVMDGFETCKNLKIQPETHDIPVIFMTAITDTNSKVEALSLGAVDYITKPFHKAEVLA
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCHHEECCHHHHHHCCHHHHHHHH
RISAHLQLRSLNKNLEKRVIERTAELNQALKDLQEYQLQLVQQEKMSVLGQLVAGLAHEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NNPVSCIYGNLGHALTYFENMDKLINLYQSYYPQPVQEIQNEISEMDLAYVRSDLPNLIF
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SMKEGIQRIRDISRSLRIFSRSDTEKKIQFDIHEGIDSTILILKHRLKGSENHPDIEIKR
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHCCCCHHHHHHHHHHCCCCCCCCEEEEC
DYGVIPQVNCFPGQLNQVFMNILANAIDALEESFSNNYYSVIDSEITVNQEMMAETPQII
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEECHHHHHHHHHHH
IQTILSEDESYALIKIKDNGIGMPQQIKERIFDNLFTTKPVGKGTGLGLSIARQIIIQKH
HHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCC
GGKLEVNSEPGRGSEFIINIPIE
CCEEEECCCCCCCCEEEEECCCC
>Mature Secondary Structure
MLDTSTETSFILVVDDTLTNLEIISEALIGAGFEVATAVDGKKALEQIQSRVPDLILLDV
CCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHCCHHHHHHH
MMPVMDGFETCKNLKIQPETHDIPVIFMTAITDTNSKVEALSLGAVDYITKPFHKAEVLA
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCHHEECCHHHHHHCCHHHHHHHH
RISAHLQLRSLNKNLEKRVIERTAELNQALKDLQEYQLQLVQQEKMSVLGQLVAGLAHEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NNPVSCIYGNLGHALTYFENMDKLINLYQSYYPQPVQEIQNEISEMDLAYVRSDLPNLIF
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SMKEGIQRIRDISRSLRIFSRSDTEKKIQFDIHEGIDSTILILKHRLKGSENHPDIEIKR
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHCCCCHHHHHHHHHHCCCCCCCCEEEEC
DYGVIPQVNCFPGQLNQVFMNILANAIDALEESFSNNYYSVIDSEITVNQEMMAETPQII
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEECHHHHHHHHHHH
IQTILSEDESYALIKIKDNGIGMPQQIKERIFDNLFTTKPVGKGTGLGLSIARQIIIQKH
HHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCC
GGKLEVNSEPGRGSEFIINIPIE
CCEEEECCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA