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The map label for this gene is pleD [H]

Identifier: 75908368

GI number: 75908368

Start: 2659158

End: 2660381

Strand: Direct

Name: pleD [H]

Synonym: Ava_2149

Alternate gene names: 75908368

Gene position: 2659158-2660381 (Clockwise)

Preceding gene: 75908367

Following gene: 75908369

Centisome position: 41.77

GC content: 36.36

Gene sequence:

>1224_bases
ATGAGCAGTCCCATAAATAACTCAGTCTTAATTGTAGATGATATCCCGACTAACATCAAGGTTTTGTTTGACATTCTCAA
TCAAGCTGGTTTTCGAGTTTCTGTTGCTAAAAATGGTCTAAGCGCGTTAGCAAAAGTACAAGAAACATTACCAAATTTAA
TTTTACTGGATGTCATGATGCCTGGTATGGATGGATTTGAAACTTGCCGCCACCTAAAAGCCAATCCCAAGACCAAGGAC
ATTCCCGTAATTTTTATGACAGCTTTATCTGATACGGTAAATAAAGTCAAAGGGTTGCAAATTGGTGCAGTTGACTATAT
TACTAAACCTATTGAATATGAAGAAGTGTTAGCGAGAATCAATGTCCATTTAGAATTGCGGCGGACTCAATTAAAGCTAG
CACAAGAGGAGAAAATGTCTTCTTTGGGACAGTTAGTGGCAGGAATTGCCCATGAAATTAATAATCCAGTTAATTTCATT
TATGGGAATTTAACCCACGCCCAAAATTATGTTGGAGACTTATTAAATTTATTAAAATTATACGAAAATCATACGGTTAA
TCCAAGTCCGGAAATTCAAGCATTCTCCAAAAGTATTGAATTAGAGTTTATTAAACAAGATTTACCACATCTATTATCTT
CGATGCAGATGGGGACTGAAAGGGTGGAGAAAATCGTGCGATCGCTGCGGTTATTCTCTCGATTAGAAGACCCAGAATTT
CAACTATTTAACTTACATGAGGGTATTGATAGCACACTCATTATTTTAAGTCACCGCCTCAAAATTTTGCCTACAGATTC
AACTATTCATATTATCAAGGAATATGGTGATATTCCTTTGGTTGAGTGCTATTCTGGAAAACTTAACCAAGTATTCATGA
ATCTGCTGGCTAATGCCATTGATGCCTTAGAAGAGTCAGTAGTTAATAGAGAAACAACTGATACATTGACAATTTGGATT
CGGACAACAGTCGTGGATGACCAAAAATCAGTATTAATTGAAATTACAGATAATGGTGTTGGTATTCCTCTAGAAGTGCA
ACAGCGTATATTTGAGCAATTTTTCACTACCAAGCCTTTAGGTAAAGGTACAGGTTTAGGATTGGCGATCGCCCATGAGA
TTATAGTCGAAAAACATGGGGGTACTCTACAGGTACAATCTACTCCGGGAAAAGGTACTCAATTTTTAATCACTATTCCC
ATTCAACAAGTGATTAGATATTAA

Upstream 100 bases:

>100_bases
TATATAGATTTATATTTTAAGATACTAAAAATGGATTTTTTTCAGGAAAGTTATTAAATAAAGATGAATCTAAAACTACG
ATGTTTCATAGGATAATTAG

Downstream 100 bases:

>100_bases
TGTTAGCTATTTTTATAGTCATGATACCCTTACTATCTGTTTAGAGACAATAATCAGGTAGTAGTAATTCCAGCTTCAGC
AGAAAAGTATTTAGGAGTCA

Product: response regulator receiver signal transduction histidine kinase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSSPINNSVLIVDDIPTNIKVLFDILNQAGFRVSVAKNGLSALAKVQETLPNLILLDVMMPGMDGFETCRHLKANPKTKD
IPVIFMTALSDTVNKVKGLQIGAVDYITKPIEYEEVLARINVHLELRRTQLKLAQEEKMSSLGQLVAGIAHEINNPVNFI
YGNLTHAQNYVGDLLNLLKLYENHTVNPSPEIQAFSKSIELEFIKQDLPHLLSSMQMGTERVEKIVRSLRLFSRLEDPEF
QLFNLHEGIDSTLIILSHRLKILPTDSTIHIIKEYGDIPLVECYSGKLNQVFMNLLANAIDALEESVVNRETTDTLTIWI
RTTVVDDQKSVLIEITDNGVGIPLEVQQRIFEQFFTTKPLGKGTGLGLAIAHEIIVEKHGGTLQVQSTPGKGTQFLITIP
IQQVIRY

Sequences:

>Translated_407_residues
MSSPINNSVLIVDDIPTNIKVLFDILNQAGFRVSVAKNGLSALAKVQETLPNLILLDVMMPGMDGFETCRHLKANPKTKD
IPVIFMTALSDTVNKVKGLQIGAVDYITKPIEYEEVLARINVHLELRRTQLKLAQEEKMSSLGQLVAGIAHEINNPVNFI
YGNLTHAQNYVGDLLNLLKLYENHTVNPSPEIQAFSKSIELEFIKQDLPHLLSSMQMGTERVEKIVRSLRLFSRLEDPEF
QLFNLHEGIDSTLIILSHRLKILPTDSTIHIIKEYGDIPLVECYSGKLNQVFMNLLANAIDALEESVVNRETTDTLTIWI
RTTVVDDQKSVLIEITDNGVGIPLEVQQRIFEQFFTTKPLGKGTGLGLAIAHEIIVEKHGGTLQVQSTPGKGTQFLITIP
IQQVIRY
>Mature_406_residues
SSPINNSVLIVDDIPTNIKVLFDILNQAGFRVSVAKNGLSALAKVQETLPNLILLDVMMPGMDGFETCRHLKANPKTKDI
PVIFMTALSDTVNKVKGLQIGAVDYITKPIEYEEVLARINVHLELRRTQLKLAQEEKMSSLGQLVAGIAHEINNPVNFIY
GNLTHAQNYVGDLLNLLKLYENHTVNPSPEIQAFSKSIELEFIKQDLPHLLSSMQMGTERVEKIVRSLRLFSRLEDPEFQ
LFNLHEGIDSTLIILSHRLKILPTDSTIHIIKEYGDIPLVECYSGKLNQVFMNLLANAIDALEESVVNRETTDTLTIWIR
TTVVDDQKSVLIEITDNGVGIPLEVQQRIFEQFFTTKPLGKGTGLGLAIAHEIIVEKHGGTLQVQSTPGKGTQFLITIPI
QQVIRY

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=276, Percent_Identity=30.0724637681159, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1790436, Length=371, Percent_Identity=26.9541778975741, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788394, Length=194, Percent_Identity=33.5051546391753, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1786599, Length=114, Percent_Identity=41.2280701754386, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790860, Length=121, Percent_Identity=39.6694214876033, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1790437, Length=114, Percent_Identity=38.5964912280702, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=36.7521367521368, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789809, Length=142, Percent_Identity=34.5070422535211, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082012, Length=100, Percent_Identity=36, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788550, Length=110, Percent_Identity=34.5454545454545, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790299, Length=110, Percent_Identity=36.3636363636364, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789149, Length=114, Percent_Identity=34.2105263157895, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787229, Length=113, Percent_Identity=32.7433628318584, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI2367329, Length=130, Percent_Identity=37.6923076923077, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI145693157, Length=303, Percent_Identity=25.4125412541254, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1786911, Length=118, Percent_Identity=34.7457627118644, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790863, Length=147, Percent_Identity=30.6122448979592, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788191, Length=113, Percent_Identity=30.9734513274336, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790300, Length=294, Percent_Identity=24.8299319727891, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI145693140, Length=103, Percent_Identity=32.0388349514563, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1788713, Length=104, Percent_Identity=38.4615384615385, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45492; Mature: 45361

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPINNSVLIVDDIPTNIKVLFDILNQAGFRVSVAKNGLSALAKVQETLPNLILLDVMM
CCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCHHEEEHHHC
PGMDGFETCRHLKANPKTKDIPVIFMTALSDTVNKVKGLQIGAVDYITKPIEYEEVLARI
CCCCHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHCCCEECCHHHHCCCCCHHHHHHHH
NVHLELRRTQLKLAQEEKMSSLGQLVAGIAHEINNPVNFIYGNLTHAQNYVGDLLNLLKL
HHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHH
YENHTVNPSPEIQAFSKSIELEFIKQDLPHLLSSMQMGTERVEKIVRSLRLFSRLEDPEF
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCE
QLFNLHEGIDSTLIILSHRLKILPTDSTIHIIKEYGDIPLVECYSGKLNQVFMNLLANAI
EEEEHHCCCCHHHHEEECCEEEEECCCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHH
DALEESVVNRETTDTLTIWIRTTVVDDQKSVLIEITDNGVGIPLEVQQRIFEQFFTTKPL
HHHHHHHHCCCCCCEEEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCC
GKGTGLGLAIAHEIIVEKHGGTLQVQSTPGKGTQFLITIPIQQVIRY
CCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECHHHHHCC
>Mature Secondary Structure 
SSPINNSVLIVDDIPTNIKVLFDILNQAGFRVSVAKNGLSALAKVQETLPNLILLDVMM
CCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCHHEEEHHHC
PGMDGFETCRHLKANPKTKDIPVIFMTALSDTVNKVKGLQIGAVDYITKPIEYEEVLARI
CCCCHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHCCCEECCHHHHCCCCCHHHHHHHH
NVHLELRRTQLKLAQEEKMSSLGQLVAGIAHEINNPVNFIYGNLTHAQNYVGDLLNLLKL
HHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHH
YENHTVNPSPEIQAFSKSIELEFIKQDLPHLLSSMQMGTERVEKIVRSLRLFSRLEDPEF
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCE
QLFNLHEGIDSTLIILSHRLKILPTDSTIHIIKEYGDIPLVECYSGKLNQVFMNLLANAI
EEEEHHCCCCHHHHEEECCEEEEECCCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHH
DALEESVVNRETTDTLTIWIRTTVVDDQKSVLIEITDNGVGIPLEVQQRIFEQFFTTKPL
HHHHHHHHCCCCCCEEEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCC
GKGTGLGLAIAHEIIVEKHGGTLQVQSTPGKGTQFLITIPIQQVIRY
CCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA