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The map label for this gene is argG

Identifier: 75908289

GI number: 75908289

Start: 2559984

End: 2561186

Strand: Direct

Name: argG

Synonym: Ava_2068

Alternate gene names: 75908289

Gene position: 2559984-2561186 (Clockwise)

Preceding gene: 75908286

Following gene: 75908291

Centisome position: 40.22

GC content: 46.55

Gene sequence:

>1203_bases
ATGGGTCGCGCCAAAAAGGTTGTTCTGGCATATTCTGGTGGAGTAGATACCTCCGTTTGCATTCCCTACCTAAAACAAGA
GTGGGGAGTAGAAGAGGTAATTACCCTAGCAGCAGATTTAGGCCAGGGAGATGAATTAGAACCAATCCGAGAAAAAGCAC
TAAAATCGGGTGCAAGTGAATCCCTCGTAGCGGATGTCAAAGAAAGTTTTATTAAAGATTATGCTTTTCCCGCGATACAG
GCCAACGCCCTCTATGAAAATCGCTATCCGCTAGGAACTGCCTTAGCCCGTCCGTTGATTGCGAAGATATTGGTCGAAGC
AGCAGAAAAATACGGTGCTGATGCGATCGCACATGGTTGTACAGGTAAAGGTAATGATCAGGTGCGGTTTGATGTCTCTT
GTACTGCTCTCAATCCTAAATTGAAGATCCTCGCCCCCGCACGGGAATGGGGTATGAGTCGGGAAGCAACCATCGCCTAC
GGTGAAAAGTTTGGCATTCCCTCACCTGTGAAAAAGTCTTCACCTTACAGCATTGATAAGAATTTACTTGGTCGCAGTAT
TGAAGCTGGTGCGCTGGAAGATCCCAAATTTGAGCCACCAGAAGAAATTTATGAGATGACAAAGGCGATCGCCGACACCC
CAAATGAGCCAGAATACATTGAAATAGGATTTACTCAAGGTCTTCCCACGACTATCAACGGTACACCCAAAGACCCTGTA
GCGCTCATCCAAGAACTAAATCAACTAGTTGGTAGTCACGGTGTCGGACGCATCGATATGATTGAAAACCGATTAGTGGG
AATCAAATCACGAGAGATTTACGAATCTCCGGCAATGTTGGTGCTAATTCAAGCCCACAGAGACCTAGAAAGCCTGACAT
TAACCGCCGATGTTAGCCACTACAAACGAGGTATTGAAGAGACCTACAGCCAAATAGTTTACAACGGCTTGTGGTACAGT
CCCCTGAAAGCCGCTTTAGATGCCTTTATTCAAAAGACTCAAGAACGGGTATCAGGAATTGTCAGAGTGAAACTATTCAA
AGGTAACGCCACCATAGTCGGACGCTGGAGCGATAGTTCACTCTACACCCCCGACTTAGCAACTTACGGCGCTGAAGACC
AATTTGACCACAAAGCCGCCGAAGGCTTCATTTACGTTTGGGGATTACCAACTCGTATCTGGGCGCAGCAGGATAGAGGT
TAA

Upstream 100 bases:

>100_bases
CCTGGTTAATTACCCTATCAGTTGTTGCACAAAGAAAATATACACGGTGTAGTTAGATATAATAATGACTCATTATTTGT
TTTCCTAACTGGAGAAAGCA

Downstream 100 bases:

>100_bases
AAACGAGGGATGAGGGAGTAGGGATAGGGAGTAAGATTCACATCTCTGAATCCCATTCCCCAATCACAAATACCCACTCC
CTATTCCCCAGCCGCGCGTT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MGRAKKVVLAYSGGVDTSVCIPYLKQEWGVEEVITLAADLGQGDELEPIREKALKSGASESLVADVKESFIKDYAFPAIQ
ANALYENRYPLGTALARPLIAKILVEAAEKYGADAIAHGCTGKGNDQVRFDVSCTALNPKLKILAPAREWGMSREATIAY
GEKFGIPSPVKKSSPYSIDKNLLGRSIEAGALEDPKFEPPEEIYEMTKAIADTPNEPEYIEIGFTQGLPTTINGTPKDPV
ALIQELNQLVGSHGVGRIDMIENRLVGIKSREIYESPAMLVLIQAHRDLESLTLTADVSHYKRGIEETYSQIVYNGLWYS
PLKAALDAFIQKTQERVSGIVRVKLFKGNATIVGRWSDSSLYTPDLATYGAEDQFDHKAAEGFIYVWGLPTRIWAQQDRG

Sequences:

>Translated_400_residues
MGRAKKVVLAYSGGVDTSVCIPYLKQEWGVEEVITLAADLGQGDELEPIREKALKSGASESLVADVKESFIKDYAFPAIQ
ANALYENRYPLGTALARPLIAKILVEAAEKYGADAIAHGCTGKGNDQVRFDVSCTALNPKLKILAPAREWGMSREATIAY
GEKFGIPSPVKKSSPYSIDKNLLGRSIEAGALEDPKFEPPEEIYEMTKAIADTPNEPEYIEIGFTQGLPTTINGTPKDPV
ALIQELNQLVGSHGVGRIDMIENRLVGIKSREIYESPAMLVLIQAHRDLESLTLTADVSHYKRGIEETYSQIVYNGLWYS
PLKAALDAFIQKTQERVSGIVRVKLFKGNATIVGRWSDSSLYTPDLATYGAEDQFDHKAAEGFIYVWGLPTRIWAQQDRG
>Mature_399_residues
GRAKKVVLAYSGGVDTSVCIPYLKQEWGVEEVITLAADLGQGDELEPIREKALKSGASESLVADVKESFIKDYAFPAIQA
NALYENRYPLGTALARPLIAKILVEAAEKYGADAIAHGCTGKGNDQVRFDVSCTALNPKLKILAPAREWGMSREATIAYG
EKFGIPSPVKKSSPYSIDKNLLGRSIEAGALEDPKFEPPEEIYEMTKAIADTPNEPEYIEIGFTQGLPTTINGTPKDPVA
LIQELNQLVGSHGVGRIDMIENRLVGIKSREIYESPAMLVLIQAHRDLESLTLTADVSHYKRGIEETYSQIVYNGLWYSP
LKAALDAFIQKTQERVSGIVRVKLFKGNATIVGRWSDSSLYTPDLATYGAEDQFDHKAAEGFIYVWGLPTRIWAQQDRG

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=402, Percent_Identity=42.2885572139304, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI16950633, Length=402, Percent_Identity=42.2885572139304, Blast_Score=313, Evalue=2e-85,
Organism=Escherichia coli, GI1789563, Length=387, Percent_Identity=29.7157622739018, Blast_Score=127, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324514, Length=410, Percent_Identity=38.5365853658537, Blast_Score=283, Evalue=3e-77,
Organism=Drosophila melanogaster, GI21358151, Length=400, Percent_Identity=41.25, Blast_Score=298, Evalue=6e-81,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_ANAVT (Q3MBE6)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_322585.1
- HSSP:   Q9X2A1
- ProteinModelPortal:   Q3MBE6
- SMR:   Q3MBE6
- STRING:   Q3MBE6
- GeneID:   3680541
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_2068
- NMPDR:   fig|240292.3.peg.1347
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- PhylomeDB:   Q3MBE6
- ProtClustDB:   PRK00509
- BioCyc:   AVAR240292:AVA_2068-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 43959; Mature: 43828

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 38-38 BINDING 89-89 BINDING 119-119 BINDING 121-121 BINDING 125-125 BINDING 125-125 BINDING 126-126 BINDING 129-129 BINDING 177-177 BINDING 186-186 BINDING 262-262 BINDING 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRAKKVVLAYSGGVDTSVCIPYLKQEWGVEEVITLAADLGQGDELEPIREKALKSGASE
CCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCH
SLVADVKESFIKDYAFPAIQANALYENRYPLGTALARPLIAKILVEAAEKYGADAIAHGC
HHHHHHHHHHHHHHCCCCHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
TGKGNDQVRFDVSCTALNPKLKILAPAREWGMSREATIAYGEKFGIPSPVKKSSPYSIDK
CCCCCCEEEEEEEEEEECCCEEEEECHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCH
NLLGRSIEAGALEDPKFEPPEEIYEMTKAIADTPNEPEYIEIGFTQGLPTTINGTPKDPV
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHH
ALIQELNQLVGSHGVGRIDMIENRLVGIKSREIYESPAMLVLIQAHRDLESLTLTADVSH
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHCCCCEEEEEECCCCHHHEEEEECHHH
YKRGIEETYSQIVYNGLWYSPLKAALDAFIQKTQERVSGIVRVKLFKGNATIVGRWSDSS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCEEEEEEECCCC
LYTPDLATYGAEDQFDHKAAEGFIYVWGLPTRIWAQQDRG
CCCCCHHHCCCCCCCCHHHHCCEEEEECCCHHHHCCCCCC
>Mature Secondary Structure 
GRAKKVVLAYSGGVDTSVCIPYLKQEWGVEEVITLAADLGQGDELEPIREKALKSGASE
CCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCH
SLVADVKESFIKDYAFPAIQANALYENRYPLGTALARPLIAKILVEAAEKYGADAIAHGC
HHHHHHHHHHHHHHCCCCHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
TGKGNDQVRFDVSCTALNPKLKILAPAREWGMSREATIAYGEKFGIPSPVKKSSPYSIDK
CCCCCCEEEEEEEEEEECCCEEEEECHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCH
NLLGRSIEAGALEDPKFEPPEEIYEMTKAIADTPNEPEYIEIGFTQGLPTTINGTPKDPV
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHH
ALIQELNQLVGSHGVGRIDMIENRLVGIKSREIYESPAMLVLIQAHRDLESLTLTADVSH
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHCCCCEEEEEECCCCHHHEEEEECHHH
YKRGIEETYSQIVYNGLWYSPLKAALDAFIQKTQERVSGIVRVKLFKGNATIVGRWSDSS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCEEEEEEECCCC
LYTPDLATYGAEDQFDHKAAEGFIYVWGLPTRIWAQQDRG
CCCCCHHHCCCCCCCCHHHHCCEEEEECCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA