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The map label for this gene is trmE

Identifier: 75908210

GI number: 75908210

Start: 2469195

End: 2470586

Strand: Direct

Name: trmE

Synonym: Ava_1989

Alternate gene names: 75908210

Gene position: 2469195-2470586 (Clockwise)

Preceding gene: 75908208

Following gene: 75908212

Centisome position: 38.79

GC content: 46.84

Gene sequence:

>1392_bases
ATGACTCAACTTTTGGCTATTACTGGAACTATTGCAGCGATCGCTACTGCTATTGTCCCTCAACAAGGTAGCGTCGGTAT
CGTGCGGGTGTCTGGTTCCCAGGCAATAGCGATCGCCCAAACTCTGTTTCATGCACCAGGTAAACAAGTTTGGGAAAGTC
ACCGGATTCTTTACGGTTACATCCGCCATCCCCAGACGCGACAAATTGTCGATGAAGCACTGTTACTGCTCATGAAAGCC
CCTCGTTCTTACACCCGCGAAGATGTGGTGGAGTTCCATTGCCACGGCGGAATTATGGCAGTGCAGCAGGTGTTGCAATT
GTGCCTGGAAGGCGGCGCTAGACTCGCTCAACCGGGGGAATTTACCCTCAGAGCCTTTTTGAATGGCAGACTAGATTTAA
CCCAAGCTGAGAGCATTGCTGATTTGGTAGGAGCGCGATCGCCCCAAGCTGCTCAAACGGCTTTAGCTGGTTTACAAGGC
AAACTCGCTCATCCCATCCGCCAGTTACGCGCTAACTGTTTGGATATTCTGGCAGAAATTGAAGCCCGGATTGATTTTGA
GGAAGATTTACCCCCGTTGGATGATGAAAAAATAATATCAGATATTGAAAACATTGCCGCAGAAATATCGCAACTACTGG
CAACTAAAGACAAAGGTGAACTGTTGCGGACAGGCTTAAAAGTGGCGATAGTCGGTCGTCCGAATGTAGGTAAATCCAGC
TTATTGAATGCTTGGAGCCAGAGCGATCGCGCCATTGTCACCGACTTACCCGGCACAACCCGCGACGTTGTGGAATCGCA
GTTAGTTGTGGGCGGTATTCCTGTACAGGTGCTAGATACAGCAGGCATTCGGGAGACTAGCGACCAAGTAGAAAAAATAG
GGGTAGAGCGATCGCGCCAAGCCGCCAACACCGCCGATTTAGTCTTGTTAACCATCGATGCAGCCACAGGTTGGACGACT
GGCGACCAAGAAATTTATGAACAAGTAAAACACCGTCCGTTAATTCTAGTAATGAATAAAATTGATTTAGTAGACAAAAA
ATTAATTACATCCTTAGAATATCCTAAAAATATTACTCAAATTGTCCACACAGCCGCAGCCCAAAAACAAGGAATTGATG
CGCTAGAAACTGCAATTTTAGAGATAGTTCAAACTGGAAAAGTGAAAGCGGCTGATATGGATTTAGCCATTAATCAAAGA
CAAGCCGCAGCCCTCACCCAGGCGAAAATTTCTCTAGAACAAGTGCAAGCCACCATCACCCAGCAGCTACCCCTAGATTT
TTGGACAATTGACTTACGCGGTGCAATTCAAGCACTAGGAGAAATCACCGGGGAAGAGGTAACGGAGTCAGTGCTTGATA
GGATTTTTAGTAGGTTTTGTATAGGAAAGTAG

Upstream 100 bases:

>100_bases
TCTGGGTACTAGGCATTGGGAAGAGGTGCAAGAGATGATGACTATTGACTGTTGACTGTTGACTATGGACTAATGACAAA
TGACCAATGACAACTGACAA

Downstream 100 bases:

>100_bases
ACGGTCAGAATTTATTGGTATGGTTTTAGGAATATTCTGCAAAAGCAACCTCTTGAGGTAATTGCTTTAAGTGAACTGGG
AATTAATTTAGATGCTGTAT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MTQLLAITGTIAAIATAIVPQQGSVGIVRVSGSQAIAIAQTLFHAPGKQVWESHRILYGYIRHPQTRQIVDEALLLLMKA
PRSYTREDVVEFHCHGGIMAVQQVLQLCLEGGARLAQPGEFTLRAFLNGRLDLTQAESIADLVGARSPQAAQTALAGLQG
KLAHPIRQLRANCLDILAEIEARIDFEEDLPPLDDEKIISDIENIAAEISQLLATKDKGELLRTGLKVAIVGRPNVGKSS
LLNAWSQSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETSDQVEKIGVERSRQAANTADLVLLTIDAATGWTT
GDQEIYEQVKHRPLILVMNKIDLVDKKLITSLEYPKNITQIVHTAAAQKQGIDALETAILEIVQTGKVKAADMDLAINQR
QAAALTQAKISLEQVQATITQQLPLDFWTIDLRGAIQALGEITGEEVTESVLDRIFSRFCIGK

Sequences:

>Translated_463_residues
MTQLLAITGTIAAIATAIVPQQGSVGIVRVSGSQAIAIAQTLFHAPGKQVWESHRILYGYIRHPQTRQIVDEALLLLMKA
PRSYTREDVVEFHCHGGIMAVQQVLQLCLEGGARLAQPGEFTLRAFLNGRLDLTQAESIADLVGARSPQAAQTALAGLQG
KLAHPIRQLRANCLDILAEIEARIDFEEDLPPLDDEKIISDIENIAAEISQLLATKDKGELLRTGLKVAIVGRPNVGKSS
LLNAWSQSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETSDQVEKIGVERSRQAANTADLVLLTIDAATGWTT
GDQEIYEQVKHRPLILVMNKIDLVDKKLITSLEYPKNITQIVHTAAAQKQGIDALETAILEIVQTGKVKAADMDLAINQR
QAAALTQAKISLEQVQATITQQLPLDFWTIDLRGAIQALGEITGEEVTESVLDRIFSRFCIGK
>Mature_462_residues
TQLLAITGTIAAIATAIVPQQGSVGIVRVSGSQAIAIAQTLFHAPGKQVWESHRILYGYIRHPQTRQIVDEALLLLMKAP
RSYTREDVVEFHCHGGIMAVQQVLQLCLEGGARLAQPGEFTLRAFLNGRLDLTQAESIADLVGARSPQAAQTALAGLQGK
LAHPIRQLRANCLDILAEIEARIDFEEDLPPLDDEKIISDIENIAAEISQLLATKDKGELLRTGLKVAIVGRPNVGKSSL
LNAWSQSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETSDQVEKIGVERSRQAANTADLVLLTIDAATGWTTG
DQEIYEQVKHRPLILVMNKIDLVDKKLITSLEYPKNITQIVHTAAAQKQGIDALETAILEIVQTGKVKAADMDLAINQRQ
AAALTQAKISLEQVQATITQQLPLDFWTIDLRGAIQALGEITGEEVTESVLDRIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=468, Percent_Identity=34.4017094017094, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI193082993, Length=457, Percent_Identity=34.3544857768052, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI193082988, Length=496, Percent_Identity=32.258064516129, Blast_Score=182, Evalue=4e-46,
Organism=Escherichia coli, GI2367268, Length=457, Percent_Identity=37.417943107221, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI87082120, Length=155, Percent_Identity=36.7741935483871, Blast_Score=88, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17507259, Length=471, Percent_Identity=31.2101910828025, Blast_Score=200, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6323665, Length=495, Percent_Identity=29.8989898989899, Blast_Score=211, Evalue=1e-55,
Organism=Drosophila melanogaster, GI45550813, Length=482, Percent_Identity=30.9128630705394, Blast_Score=195, Evalue=5e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_ANAVT (Q3MBM5)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_322506.1
- HSSP:   Q9WYA4
- ProteinModelPortal:   Q3MBM5
- SMR:   Q3MBM5
- STRING:   Q3MBM5
- GeneID:   3681415
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_1989
- NMPDR:   fig|240292.3.peg.3069
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q3MBM5
- ProtClustDB:   PRK05291
- BioCyc:   AVAR240292:AVA_1989-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00449
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 50257; Mature: 50126

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: BINDING 29-29 BINDING 91-91 BINDING 130-130 BINDING 463-463

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLLAITGTIAAIATAIVPQQGSVGIVRVSGSQAIAIAQTLFHAPGKQVWESHRILYGY
CCCEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHCCCCEEHH
IRHPQTRQIVDEALLLLMKAPRSYTREDVVEFHCHGGIMAVQQVLQLCLEGGARLAQPGE
HCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCH
FTLRAFLNGRLDLTQAESIADLVGARSPQAAQTALAGLQGKLAHPIRQLRANCLDILAEI
HHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
EARIDFEEDLPPLDDEKIISDIENIAAEISQLLATKDKGELLRTGLKVAIVGRPNVGKSS
HHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCHHH
LLNAWSQSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETSDQVEKIGVERSRQ
HHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHH
AANTADLVLLTIDAATGWTTGDQEIYEQVKHRPLILVMNKIDLVDKKLITSLEYPKNITQ
HCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCHHHHH
IVHTAAAQKQGIDALETAILEIVQTGKVKAADMDLAINQRQAAALTQAKISLEQVQATIT
HHHHHHHHHCCHHHHHHHHHHHHHCCCCEEECCCCEECCHHHHHHHHHHHHHHHHHHHHH
QQLPLDFWTIDLRGAIQALGEITGEEVTESVLDRIFSRFCIGK
HHCCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQLLAITGTIAAIATAIVPQQGSVGIVRVSGSQAIAIAQTLFHAPGKQVWESHRILYGY
CCEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHCCCCEEHH
IRHPQTRQIVDEALLLLMKAPRSYTREDVVEFHCHGGIMAVQQVLQLCLEGGARLAQPGE
HCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCH
FTLRAFLNGRLDLTQAESIADLVGARSPQAAQTALAGLQGKLAHPIRQLRANCLDILAEI
HHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
EARIDFEEDLPPLDDEKIISDIENIAAEISQLLATKDKGELLRTGLKVAIVGRPNVGKSS
HHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCHHH
LLNAWSQSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETSDQVEKIGVERSRQ
HHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHH
AANTADLVLLTIDAATGWTTGDQEIYEQVKHRPLILVMNKIDLVDKKLITSLEYPKNITQ
HCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCHHHHH
IVHTAAAQKQGIDALETAILEIVQTGKVKAADMDLAINQRQAAALTQAKISLEQVQATIT
HHHHHHHHHCCHHHHHHHHHHHHHCCCCEEECCCCEECCHHHHHHHHHHHHHHHHHHHHH
QQLPLDFWTIDLRGAIQALGEITGEEVTESVLDRIFSRFCIGK
HHCCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA