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The map label for this gene is ndhK

Identifier: 75908080

GI number: 75908080

Start: 2312735

End: 2313472

Strand: Direct

Name: ndhK

Synonym: Ava_1859

Alternate gene names: 75908080

Gene position: 2312735-2313472 (Clockwise)

Preceding gene: 75908079

Following gene: 75908081

Centisome position: 36.33

GC content: 46.88

Gene sequence:

>738_bases
ATGGTCTTGAATTCTGATTTAACTACTCAGGACAAAGAGCGCATCATCAACCCCATTGAACGTCCTACAGTCACTCAAGA
CCTTTCGGAAAACGTTATTTTAACCACGGTTGATGACCTCTACAACTGGGCTAGGCTTTCGAGTCTGTGGCCGTTGTTAT
TTGGTACTGCTTGCTGCTTTATTGAGTTTGCAGCCCTAATTGGTTCCCGTTTTGACTTTGACCGCTTCGGGTTAATTCCC
CGTTCCAGTCCCCGTCAAGCCGATTTGATTATTACAGCTGGAACAATCACCATGAAGATGGCTCCTCAATTGGTGCGTCT
TTATGAACAAATGCCCGAACCTAAGTATGTAATTGCGATGGGCGCTTGTACAATTACGGGCGGGATGTTCAGTGTCGATT
CACCTACAGCTGTGCGTGGAGTTGACAAGTTAATTCCCGTGGATGTCTACTTACCTGGTTGTCCTCCCCGTCCTGAAGCA
ATTATCGACGCAATTATTAAGCTGCGGAAGAAGATTGCTAACGATTCCATGCAGGAACGGAGTCTGATTCGCCAAACCCA
CCGTTTCTACAGCACGACTCATAACTTGAAGCCAGTGGCAGAAATCTTAACTGGTAAGTATATGCAGTCTGAGACTCGCT
TCAACCCACCAAAAGAATTGACAGAAGCGATCGGTCTTCCTGTTCCCCCAGCGCTGCTGACATCACAAACACAGAAGGAG
GAACAAAAACGTGGCTGA

Upstream 100 bases:

>100_bases
CTTTTCACCGTCTGGGGCTATTGGCATTCATTGAAGCGCTGATTTTTATTGCAATTCTTGTAGTCGCCCTAGTTTACGCA
TGGCGTAAAGGAGCATTGGA

Downstream 100 bases:

>100_bases
TGAAGAATTAAAACCAGTACCCGCAGCAGCAGAGGCTATAGTTCCATCTGGGCCGACTTCTCAGTGGCTAACGGAAAATG
GCTTTGCTCATGAATCCTTG

Product: NADH dehydrogenase subunit B

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit K; NDH-1 subunit K; NDH-K

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MVLNSDLTTQDKERIINPIERPTVTQDLSENVILTTVDDLYNWARLSSLWPLLFGTACCFIEFAALIGSRFDFDRFGLIP
RSSPRQADLIITAGTITMKMAPQLVRLYEQMPEPKYVIAMGACTITGGMFSVDSPTAVRGVDKLIPVDVYLPGCPPRPEA
IIDAIIKLRKKIANDSMQERSLIRQTHRFYSTTHNLKPVAEILTGKYMQSETRFNPPKELTEAIGLPVPPALLTSQTQKE
EQKRG

Sequences:

>Translated_245_residues
MVLNSDLTTQDKERIINPIERPTVTQDLSENVILTTVDDLYNWARLSSLWPLLFGTACCFIEFAALIGSRFDFDRFGLIP
RSSPRQADLIITAGTITMKMAPQLVRLYEQMPEPKYVIAMGACTITGGMFSVDSPTAVRGVDKLIPVDVYLPGCPPRPEA
IIDAIIKLRKKIANDSMQERSLIRQTHRFYSTTHNLKPVAEILTGKYMQSETRFNPPKELTEAIGLPVPPALLTSQTQKE
EQKRG
>Mature_245_residues
MVLNSDLTTQDKERIINPIERPTVTQDLSENVILTTVDDLYNWARLSSLWPLLFGTACCFIEFAALIGSRFDFDRFGLIP
RSSPRQADLIITAGTITMKMAPQLVRLYEQMPEPKYVIAMGACTITGGMFSVDSPTAVRGVDKLIPVDVYLPGCPPRPEA
IIDAIIKLRKKIANDSMQERSLIRQTHRFYSTTHNLKPVAEILTGKYMQSETRFNPPKELTEAIGLPVPPALLTSQTQKE
EQKRG

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=143, Percent_Identity=51.0489510489511, Blast_Score=175, Evalue=3e-44,
Organism=Escherichia coli, GI1788624, Length=173, Percent_Identity=46.242774566474, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1788834, Length=111, Percent_Identity=40.5405405405405, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789074, Length=108, Percent_Identity=41.6666666666667, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=139, Percent_Identity=51.0791366906475, Blast_Score=163, Evalue=9e-41,
Organism=Drosophila melanogaster, GI18859983, Length=139, Percent_Identity=52.5179856115108, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24642371, Length=139, Percent_Identity=52.5179856115108, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24651058, Length=136, Percent_Identity=52.2058823529412, Blast_Score=168, Evalue=3e-42,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NDHK_ANAVT (Q9XBL7)

Other databases:

- EMBL:   AJ012181
- EMBL:   CP000117
- RefSeq:   YP_322376.1
- ProteinModelPortal:   Q9XBL7
- SMR:   Q9XBL7
- STRING:   Q9XBL7
- GeneID:   3681811
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_1859
- NMPDR:   fig|240292.3.peg.2939
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   ITMKMAP
- PhylomeDB:   Q9XBL7
- ProtClustDB:   PRK06411
- BioCyc:   AVAR240292:AVA_1859-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: 1.6.5.3

Molecular weight: Translated: 27467; Mature: 27467

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLNSDLTTQDKERIINPIERPTVTQDLSENVILTTVDDLYNWARLSSLWPLLFGTACCF
CCCCCCCCCCHHHHHHCCCCCCCCHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
IEFAALIGSRFDFDRFGLIPRSSPRQADLIITAGTITMKMAPQLVRLYEQMPEPKYVIAM
HHHHHHHCCCCCHHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEE
GACTITGGMFSVDSPTAVRGVDKLIPVDVYLPGCPPRPEAIIDAIIKLRKKIANDSMQER
CHHHHCCCEEECCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHH
SLIRQTHRFYSTTHNLKPVAEILTGKYMQSETRFNPPKELTEAIGLPVPPALLTSQTQKE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
EQKRG
HHHCC
>Mature Secondary Structure
MVLNSDLTTQDKERIINPIERPTVTQDLSENVILTTVDDLYNWARLSSLWPLLFGTACCF
CCCCCCCCCCHHHHHHCCCCCCCCHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
IEFAALIGSRFDFDRFGLIPRSSPRQADLIITAGTITMKMAPQLVRLYEQMPEPKYVIAM
HHHHHHHCCCCCHHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEE
GACTITGGMFSVDSPTAVRGVDKLIPVDVYLPGCPPRPEAIIDAIIKLRKKIANDSMQER
CHHHHCCCEEECCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHH
SLIRQTHRFYSTTHNLKPVAEILTGKYMQSETRFNPPKELTEAIGLPVPPALLTSQTQKE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
EQKRG
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA