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The map label for this gene is groEL [H]

Identifier: 75676377

GI number: 75676377

Start: 2385288

End: 2386925

Strand: Direct

Name: groEL [H]

Synonym: Nwi_2192

Alternate gene names: 75676377

Gene position: 2385288-2386925 (Clockwise)

Preceding gene: 75676376

Following gene: 75676381

Centisome position: 70.11

GC content: 64.59

Gene sequence:

>1638_bases
ATGTCAGCCAAAGATGTCAAATTCGGCGTCGACGCTCGCGACAAGATGCTGCGCGGCGTGGACATCCTCGCCAATGCCGT
GAAGGTGACGCTGGGTCCGAAGGGCCGCAACGTCGTGCTCGAAAAGTCGTTCGGCGCGCCCCGCATCACCAAGGACGGCG
TCACCGTCGCCAAGGAAATTGAGCTTGACGACAAGTTCGAGAACATGGGCGCGCAGATGGTGCGCGAAGTCGCCTCCAAG
TCAGCAGACGCGGCCGGCGACGGCACCACCACCGCGACGGTTCTGGCCCAGGCAATCGTGAAGGAAGGCGCCAAGTCGGT
CGCCGCCGGCATGAACCCGATGGACCTGAAGCGTGGTATTGATCTCGCCGTCGAAGCCGTGGTCGCAGACCTCAGCCGCA
ACTCCAAGAAAGTCACCTCGAACGAGGAAATCGCCCAGGTCGGCACCATCTCGGCCAACGGCGATTCCGAAATCGGCAAG
TTCCTCGCCGACGCCATGAAGAAGGTCGGCAACGAGGGTGTCATCACCGTCGAGGAAGCCAAGTCGCTGGAGACCGAACT
CGATGTCGTCGAGGGCATGCAGTTCGACCGCGGCTACATCTCGCCCTACTTCGTCACCAACGCCGAAAAGATGCGCGTCG
AGATGGACGACGCCTACATCCTGATCAACGAGAAGAAGCTGTCCTCGCTGAATGAACTGCTGCCGCTGCTCGAAGCCGTG
GTGCAGACCGGCAAACCGCTGGTTATCGTCGCGGAAGACGTCGAGGGCGAGGCTCTGGCGACGCTGGTGGTCAACCGCCT
GCGCGGCGGCCTCAAGGTCGCGGCCGTCAAGGCGCCAGGCTTCGGCGACCGCCGCAAGGCCATGCTGCAGGACATCGCGA
TCCTGACCGGCGGTCAGGCGATCAGCGAAGACCTCGGCATCAAGCTCGAGAACGTCACGCTGCAGATGCTCGGCCGCGCC
AAGAAGGTGATGATCGACAAGGAAAACACCACGATCGTCAACGGCGCCGGCAAGAAGGCCGACATCGAGGCGCGCGTGGC
GCAGATCAAGGCGCAGATCGAGGAGACCACCTCGGACTACGACCGCGAGAAGCTGCAGGAGCGTCTGGCCAAGCTCGCGG
GCGGCGTCGCGGTGATCCGCGTCGGCGGCGCGACCGAGGTCGAGGTGAAGGAGCGCAAGGATCGCGTGGACGACGCGATG
CACGCGACCCGCGCGGCGGTCGAGGAAGGCATCGTGCCGGGCGGCGGCGTCGCCCTGCTCCGTGCTTCCGAGCAGCTCAA
GCGCATCAAGACCCAGAACGACGACCAGAAGACCGGCGTCGAGATCGTGCGCAAGGCGCTGTCCGCGCCGGCCCGCCAGA
TCGCGATCAACGCGGGCGAGGACGGCAGCGTCATCGTCGGCAAGATCCTCGAGAAGGAGCAGTATTCCTACGGCTTCGAC
TCGCAGACCGGCGACTACGGCAACCTGATCTCCAAGGGCATCATCGACCCGACCAAGGTCGTGCGCACCGCGATCCAGAA
CGCCGCATCCGTCGCCTCGCTTCTGATCACCACCGAAGCGATGGTCGCCGAACTGCCGAAGAAGAACAGCCCTGCCCCGG
CGATGCCGGGCGGCGGCATGGGCGGCATGGATTTCTGA

Upstream 100 bases:

>100_bases
GAATCCGACATCATGGGCGTCCTCACCGACGTCGCGCCCGCCAAGAAGAAGGCCGCCTGAGCGCCATTCCCTGCACGATC
ATTCAAAGGAACAACTCAAC

Downstream 100 bases:

>100_bases
TCCACTCGTTCGAGCAATTCACGAAATGCAAACCCCGGCAGCGATGCCGGGGTTTTCATTTTTACAAGCTCGAGACTCGT
CACGCCGAACGCGATGCAGA

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein 2; Protein Cpn60 2 [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MSAKDVKFGVDARDKMLRGVDILANAVKVTLGPKGRNVVLEKSFGAPRITKDGVTVAKEIELDDKFENMGAQMVREVASK
SADAAGDGTTTATVLAQAIVKEGAKSVAAGMNPMDLKRGIDLAVEAVVADLSRNSKKVTSNEEIAQVGTISANGDSEIGK
FLADAMKKVGNEGVITVEEAKSLETELDVVEGMQFDRGYISPYFVTNAEKMRVEMDDAYILINEKKLSSLNELLPLLEAV
VQTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQAISEDLGIKLENVTLQMLGRA
KKVMIDKENTTIVNGAGKKADIEARVAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAM
HATRAAVEEGIVPGGGVALLRASEQLKRIKTQNDDQKTGVEIVRKALSAPARQIAINAGEDGSVIVGKILEKEQYSYGFD
SQTGDYGNLISKGIIDPTKVVRTAIQNAASVASLLITTEAMVAELPKKNSPAPAMPGGGMGGMDF

Sequences:

>Translated_545_residues
MSAKDVKFGVDARDKMLRGVDILANAVKVTLGPKGRNVVLEKSFGAPRITKDGVTVAKEIELDDKFENMGAQMVREVASK
SADAAGDGTTTATVLAQAIVKEGAKSVAAGMNPMDLKRGIDLAVEAVVADLSRNSKKVTSNEEIAQVGTISANGDSEIGK
FLADAMKKVGNEGVITVEEAKSLETELDVVEGMQFDRGYISPYFVTNAEKMRVEMDDAYILINEKKLSSLNELLPLLEAV
VQTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQAISEDLGIKLENVTLQMLGRA
KKVMIDKENTTIVNGAGKKADIEARVAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAM
HATRAAVEEGIVPGGGVALLRASEQLKRIKTQNDDQKTGVEIVRKALSAPARQIAINAGEDGSVIVGKILEKEQYSYGFD
SQTGDYGNLISKGIIDPTKVVRTAIQNAASVASLLITTEAMVAELPKKNSPAPAMPGGGMGGMDF
>Mature_544_residues
SAKDVKFGVDARDKMLRGVDILANAVKVTLGPKGRNVVLEKSFGAPRITKDGVTVAKEIELDDKFENMGAQMVREVASKS
ADAAGDGTTTATVLAQAIVKEGAKSVAAGMNPMDLKRGIDLAVEAVVADLSRNSKKVTSNEEIAQVGTISANGDSEIGKF
LADAMKKVGNEGVITVEEAKSLETELDVVEGMQFDRGYISPYFVTNAEKMRVEMDDAYILINEKKLSSLNELLPLLEAVV
QTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQAISEDLGIKLENVTLQMLGRAK
KVMIDKENTTIVNGAGKKADIEARVAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAMH
ATRAAVEEGIVPGGGVALLRASEQLKRIKTQNDDQKTGVEIVRKALSAPARQIAINAGEDGSVIVGKILEKEQYSYGFDS
QTGDYGNLISKGIIDPTKVVRTAIQNAASVASLLITTEAMVAELPKKNSPAPAMPGGGMGGMDF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=527, Percent_Identity=56.7362428842505, Blast_Score=597, Evalue=1e-170,
Organism=Homo sapiens, GI31542947, Length=527, Percent_Identity=56.7362428842505, Blast_Score=597, Evalue=1e-170,
Organism=Homo sapiens, GI5453607, Length=547, Percent_Identity=22.8519195612431, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1790586, Length=530, Percent_Identity=66.4150943396226, Blast_Score=701, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=529, Percent_Identity=55.5765595463138, Blast_Score=584, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI193210679, Length=240, Percent_Identity=50.8333333333333, Blast_Score=228, Evalue=7e-60,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=57.6045627376426, Blast_Score=612, Evalue=1e-176,
Organism=Saccharomyces cerevisiae, GI6322350, Length=545, Percent_Identity=24.0366972477064, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322524, Length=189, Percent_Identity=24.8677248677249, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24641193, Length=528, Percent_Identity=59.469696969697, Blast_Score=622, Evalue=1e-178,
Organism=Drosophila melanogaster, GI24641191, Length=528, Percent_Identity=59.469696969697, Blast_Score=622, Evalue=1e-178,
Organism=Drosophila melanogaster, GI45550936, Length=525, Percent_Identity=55.4285714285714, Blast_Score=584, Evalue=1e-167,
Organism=Drosophila melanogaster, GI45550132, Length=525, Percent_Identity=55.4285714285714, Blast_Score=584, Evalue=1e-167,
Organism=Drosophila melanogaster, GI45550935, Length=525, Percent_Identity=55.4285714285714, Blast_Score=584, Evalue=1e-167,
Organism=Drosophila melanogaster, GI17864606, Length=546, Percent_Identity=47.0695970695971, Blast_Score=497, Evalue=1e-141,
Organism=Drosophila melanogaster, GI24584129, Length=537, Percent_Identity=36.4990689013035, Blast_Score=349, Evalue=3e-96,
Organism=Drosophila melanogaster, GI19921262, Length=537, Percent_Identity=36.4990689013035, Blast_Score=349, Evalue=3e-96,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57873; Mature: 57742

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKDVKFGVDARDKMLRGVDILANAVKVTLGPKGRNVVLEKSFGAPRITKDGVTVAKEI
CCCCCCEECCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCEEEEC
ELDDKFENMGAQMVREVASKSADAAGDGTTTATVLAQAIVKEGAKSVAAGMNPMDLKRGI
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCH
DLAVEAVVADLSRNSKKVTSNEEIAQVGTISANGDSEIGKFLADAMKKVGNEGVITVEEA
HHHHHHHHHHHHCCCCCCCCCCHHHHEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEHHH
KSLETELDVVEGMQFDRGYISPYFVTNAEKMRVEMDDAYILINEKKLSSLNELLPLLEAV
HHHHHHHHHHHCCCCCCCCCCCEEEECHHHEEEEECCEEEEEECHHHHHHHHHHHHHHHH
VQTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQA
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHH
ISEDLGIKLENVTLQMLGRAKKVMIDKENTTIVNGAGKKADIEARVAQIKAQIEETTSDY
HHHHHCCEEEHHHHHHHCCCHHEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAMHATRAAVEEGIVPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RASEQLKRIKTQNDDQKTGVEIVRKALSAPARQIAINAGEDGSVIVGKILEKEQYSYGFD
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEHHHHHHHHHCCCCCC
SQTGDYGNLISKGIIDPTKVVRTAIQNAASVASLLITTEAMVAELPKKNSPAPAMPGGGM
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GGMDF
CCCCC
>Mature Secondary Structure 
SAKDVKFGVDARDKMLRGVDILANAVKVTLGPKGRNVVLEKSFGAPRITKDGVTVAKEI
CCCCCEECCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCEEEEC
ELDDKFENMGAQMVREVASKSADAAGDGTTTATVLAQAIVKEGAKSVAAGMNPMDLKRGI
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCH
DLAVEAVVADLSRNSKKVTSNEEIAQVGTISANGDSEIGKFLADAMKKVGNEGVITVEEA
HHHHHHHHHHHHCCCCCCCCCCHHHHEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEHHH
KSLETELDVVEGMQFDRGYISPYFVTNAEKMRVEMDDAYILINEKKLSSLNELLPLLEAV
HHHHHHHHHHHCCCCCCCCCCCEEEECHHHEEEEECCEEEEEECHHHHHHHHHHHHHHHH
VQTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQA
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHH
ISEDLGIKLENVTLQMLGRAKKVMIDKENTTIVNGAGKKADIEARVAQIKAQIEETTSDY
HHHHHCCEEEHHHHHHHCCCHHEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAMHATRAAVEEGIVPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RASEQLKRIKTQNDDQKTGVEIVRKALSAPARQIAINAGEDGSVIVGKILEKEQYSYGFD
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEHHHHHHHHHCCCCCC
SQTGDYGNLISKGIIDPTKVVRTAIQNAASVASLLITTEAMVAELPKKNSPAPAMPGGGM
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GGMDF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8101485; 12597275 [H]