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The map label for this gene is thrS [H]

Identifier: 75676225

GI number: 75676225

Start: 2224074

End: 2226134

Strand: Direct

Name: thrS [H]

Synonym: Nwi_2035

Alternate gene names: 75676225

Gene position: 2224074-2226134 (Clockwise)

Preceding gene: 75676224

Following gene: 75676226

Centisome position: 65.37

GC content: 63.27

Gene sequence:

>2061_bases
ATGTCCGAAAACCAAGACCCCGCCGGATTCCGGTACGGCCTCGCCAACCTGAAACCCGTCTCGCCCGCCGACAACGTCGT
CCTCACCTTCCCCGACGGCGCGACACGCGAATTCCCGAAGAATACCACCGGCCTGGAAATCGCCAAGGGGATCTCGCCGT
CGCTGGCCAAGCGCACGGTGGCCATGGCGCTCGACGGCATCGTCAACGATCTCGCCGACCCGATCGAACAGGACGCTACA
ATTGAATTCATCAGCCGCGACGACCCTCGCGCGCTGGAACTGATCCGCCATGACGCCGCGCATGTGCTGGCGGAAGCCGT
GCAGACATTGTGGCCGGGCACGCAGGTCACCATCGGCCCGGTGATCGAGAACGGCTTCTATTACGACTTCTTCCGCAACG
AGCCGTTCTCGGTTGAGGATTTCGCCGCGATCGAGAAGAAGATGCGCGAGATCATCGCGCGCGACCAGCCCTTCACCAAG
GATGTCTGGGACCGCGAGAAGACCAAGCAGGTGTTTCGCGACAAGGGCGAGGCCTTCAAGGTCGAACTCGTCGACGCCAT
CCCCGGCAACGAGCCGATCCGGATCTACTACCAGGGTGACTGGTTCGATCTCTGCCGCGGCCCGCACATGACCTCGACCG
GCAAGATCGGCAACGCGTTCAAGCTGATGAAGGTGGCCGGCGCTTACTGGCGCGGCGACAGCAACAATCCGATGCTGACG
CGCATCTATGGCACCGCCTTCGCGAACCAGGCCGATCTCGACGCATACCTCAAGCAGATCGAGGAAGCCGAGAAGCGCGA
CCACCGAAAGCTCGGCCGCGAACTCGACCTGTTCCACTTTCAGGAGGAAGGTCCGGGCGTGGTGTTCTGGCACGCCAAAG
GCTGGACCATCTTCCAGGCCGTGATCGCCTACATGCGCCGGCGGCTCGCGGAAGATTATGACGAGGTCAACGCGCCGCAG
ATGCTCGACAAGTCGCTGTGGGAGACGTCGGGACATTGGGAATGGTATCGCGAGAACATGTTCGCCGCGCAGTCCGCCGG
CGATGAAGCCGAGGACAAGCGCTGGTTCACAATCAAGCCGATGAACTGTCCAGGCCATGTGCAACTCTTCAAGCACGGAC
TCAAAAGCTACCGCGACCTGCCGCTGCGGCTCGCTGAATTCGGCGTCGTGCATCGCTACGAGCCGTCGGGCGCAATGCAC
GGCCTGATGCGGGTGCGCGGCTTCACCCAGGACGACGCGCATGTTTTCTGCACCGAGGAGCAGCTCGCCGACGAGTGCCT
CAAGATCAACGAGCTGATCTTGTCGACCTATGCCGATTTCGGCTTCGACGGTGAGCTGACGGTGAAGCTTTCGACGCGTC
CCGAGAAGCGCGTGGGCTCGGACGCGGCGTGGGATCACGCCGAAGCGGTGATGGCGGATGTCCTCAAGCACATCGCGGCA
TCGAACAACCGCATCAGGACCGAGACCAATCCCGGCGAGGGTGCGTTCTACGGGCCGAAGTTCGAGTACGTCCTGCGCGA
TGCCATCGGCCGCGACTGGCAGTGCGGCACGACCCAGGTGGACTTCAACCTGCCGGAGCGGTTCGGCGCCTTCTACATCG
ACGCGGACGGCGGCAAGAAGGCGCCGGTGATGGTGCATCGCGCGATCTGCGGATCGATCGAGCGCTTCATCGGCATCCTG
ATCGAGCACTTCGCCGGCCACTTTCCGCTCTGGCTCGCGCCGGTGCAGGCGGTCGTCACCACCATTACGTCGGACGGCGA
TGAATACGCGAAAGTGGTCGCGTCCGCCGCGCGGCGCGCGGGGCTTCGCGTCGAACTGGACCTGCGCAACGAAAAGATCA
ACTACAAGGTGCGCGAGCACTCGCTGGCGAAGATCCCCGCCTTGCTCGTTGTCGGCAAAAAGGAGGCCGAGACCCAATCG
GTGTCCATCCGCCGGCTCGGCAGCGACGGCCAGAAAGTGACGCCGACCGGCGAGGCCATTGCCGCGCTTGTAGCGGAAGC
CGTTCCGCCGGATATCCAACGCATCAAGACAGCGCCCCAGAGCCTTTCCGCTTCTGATTGA

Upstream 100 bases:

>100_bases
CCATGGGCCTTCACGGGTTGCACGCCGCAAATCGGCTGCTATACCGCAGCATCCCGCTTACCTGCGCTCGTTCGCAGGAG
TGAGCCACAGGAGAGAGTGA

Downstream 100 bases:

>100_bases
ATCAGAAGCGGGGCTCTATAATATTGTTTTGACGCGTTTTCTTCGCGCGAACCGGTGTCCGCTTCGCTCGAAAACGCTAT
AGAGCATGATCCCGACCCGA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 686; Mature: 685

Protein sequence:

>686_residues
MSENQDPAGFRYGLANLKPVSPADNVVLTFPDGATREFPKNTTGLEIAKGISPSLAKRTVAMALDGIVNDLADPIEQDAT
IEFISRDDPRALELIRHDAAHVLAEAVQTLWPGTQVTIGPVIENGFYYDFFRNEPFSVEDFAAIEKKMREIIARDQPFTK
DVWDREKTKQVFRDKGEAFKVELVDAIPGNEPIRIYYQGDWFDLCRGPHMTSTGKIGNAFKLMKVAGAYWRGDSNNPMLT
RIYGTAFANQADLDAYLKQIEEAEKRDHRKLGRELDLFHFQEEGPGVVFWHAKGWTIFQAVIAYMRRRLAEDYDEVNAPQ
MLDKSLWETSGHWEWYRENMFAAQSAGDEAEDKRWFTIKPMNCPGHVQLFKHGLKSYRDLPLRLAEFGVVHRYEPSGAMH
GLMRVRGFTQDDAHVFCTEEQLADECLKINELILSTYADFGFDGELTVKLSTRPEKRVGSDAAWDHAEAVMADVLKHIAA
SNNRIRTETNPGEGAFYGPKFEYVLRDAIGRDWQCGTTQVDFNLPERFGAFYIDADGGKKAPVMVHRAICGSIERFIGIL
IEHFAGHFPLWLAPVQAVVTTITSDGDEYAKVVASAARRAGLRVELDLRNEKINYKVREHSLAKIPALLVVGKKEAETQS
VSIRRLGSDGQKVTPTGEAIAALVAEAVPPDIQRIKTAPQSLSASD

Sequences:

>Translated_686_residues
MSENQDPAGFRYGLANLKPVSPADNVVLTFPDGATREFPKNTTGLEIAKGISPSLAKRTVAMALDGIVNDLADPIEQDAT
IEFISRDDPRALELIRHDAAHVLAEAVQTLWPGTQVTIGPVIENGFYYDFFRNEPFSVEDFAAIEKKMREIIARDQPFTK
DVWDREKTKQVFRDKGEAFKVELVDAIPGNEPIRIYYQGDWFDLCRGPHMTSTGKIGNAFKLMKVAGAYWRGDSNNPMLT
RIYGTAFANQADLDAYLKQIEEAEKRDHRKLGRELDLFHFQEEGPGVVFWHAKGWTIFQAVIAYMRRRLAEDYDEVNAPQ
MLDKSLWETSGHWEWYRENMFAAQSAGDEAEDKRWFTIKPMNCPGHVQLFKHGLKSYRDLPLRLAEFGVVHRYEPSGAMH
GLMRVRGFTQDDAHVFCTEEQLADECLKINELILSTYADFGFDGELTVKLSTRPEKRVGSDAAWDHAEAVMADVLKHIAA
SNNRIRTETNPGEGAFYGPKFEYVLRDAIGRDWQCGTTQVDFNLPERFGAFYIDADGGKKAPVMVHRAICGSIERFIGIL
IEHFAGHFPLWLAPVQAVVTTITSDGDEYAKVVASAARRAGLRVELDLRNEKINYKVREHSLAKIPALLVVGKKEAETQS
VSIRRLGSDGQKVTPTGEAIAALVAEAVPPDIQRIKTAPQSLSASD
>Mature_685_residues
SENQDPAGFRYGLANLKPVSPADNVVLTFPDGATREFPKNTTGLEIAKGISPSLAKRTVAMALDGIVNDLADPIEQDATI
EFISRDDPRALELIRHDAAHVLAEAVQTLWPGTQVTIGPVIENGFYYDFFRNEPFSVEDFAAIEKKMREIIARDQPFTKD
VWDREKTKQVFRDKGEAFKVELVDAIPGNEPIRIYYQGDWFDLCRGPHMTSTGKIGNAFKLMKVAGAYWRGDSNNPMLTR
IYGTAFANQADLDAYLKQIEEAEKRDHRKLGRELDLFHFQEEGPGVVFWHAKGWTIFQAVIAYMRRRLAEDYDEVNAPQM
LDKSLWETSGHWEWYRENMFAAQSAGDEAEDKRWFTIKPMNCPGHVQLFKHGLKSYRDLPLRLAEFGVVHRYEPSGAMHG
LMRVRGFTQDDAHVFCTEEQLADECLKINELILSTYADFGFDGELTVKLSTRPEKRVGSDAAWDHAEAVMADVLKHIAAS
NNRIRTETNPGEGAFYGPKFEYVLRDAIGRDWQCGTTQVDFNLPERFGAFYIDADGGKKAPVMVHRAICGSIERFIGILI
EHFAGHFPLWLAPVQAVVTTITSDGDEYAKVVASAARRAGLRVELDLRNEKINYKVREHSLAKIPALLVVGKKEAETQSV
SIRRLGSDGQKVTPTGEAIAALVAEAVPPDIQRIKTAPQSLSASD

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=624, Percent_Identity=41.1858974358974, Blast_Score=462, Evalue=1e-130,
Organism=Homo sapiens, GI38570111, Length=607, Percent_Identity=39.5387149917628, Blast_Score=444, Evalue=1e-124,
Organism=Homo sapiens, GI20070344, Length=619, Percent_Identity=37.9644588045234, Blast_Score=418, Evalue=1e-116,
Organism=Escherichia coli, GI1788013, Length=626, Percent_Identity=50.7987220447284, Blast_Score=666, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71984184, Length=627, Percent_Identity=38.9154704944179, Blast_Score=447, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI71984192, Length=627, Percent_Identity=38.9154704944179, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322112, Length=657, Percent_Identity=38.0517503805175, Blast_Score=437, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6322654, Length=396, Percent_Identity=35.1010101010101, Blast_Score=271, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24583839, Length=642, Percent_Identity=39.0965732087227, Blast_Score=460, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24583842, Length=642, Percent_Identity=39.0965732087227, Blast_Score=460, Evalue=1e-129,
Organism=Drosophila melanogaster, GI28574141, Length=642, Percent_Identity=39.0965732087227, Blast_Score=460, Evalue=1e-129,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 76989; Mature: 76858

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENQDPAGFRYGLANLKPVSPADNVVLTFPDGATREFPKNTTGLEIAKGISPSLAKRTV
CCCCCCCCCCEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHCCCCHHHHHHHH
AMALDGIVNDLADPIEQDATIEFISRDDPRALELIRHDAAHVLAEAVQTLWPGTQVTIGP
HHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
VIENGFYYDFFRNEPFSVEDFAAIEKKMREIIARDQPFTKDVWDREKTKQVFRDKGEAFK
EECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEE
VELVDAIPGNEPIRIYYQGDWFDLCRGPHMTSTGKIGNAFKLMKVAGAYWRGDSNNPMLT
EEEEECCCCCCCEEEEECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEE
RIYGTAFANQADLDAYLKQIEEAEKRDHRKLGRELDLFHFQEEGPGVVFWHAKGWTIFQA
EEECHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCHHHHHH
VIAYMRRRLAEDYDEVNAPQMLDKSLWETSGHWEWYRENMFAAQSAGDEAEDKRWFTIKP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEC
MNCPGHVQLFKHGLKSYRDLPLRLAEFGVVHRYEPSGAMHGLMRVRGFTQDDAHVFCTEE
CCCCCHHHHHHHHHHHHHCCCHHHHHHCCEEEECCCHHHHHHHHHCCCCCCCCEEEECHH
QLADECLKINELILSTYADFGFDGELTVKLSTRPEKRVGSDAAWDHAEAVMADVLKHIAA
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHHHHC
SNNRIRTETNPGEGAFYGPKFEYVLRDAIGRDWQCGTTQVDFNLPERFGAFYIDADGGKK
CCCEEEECCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEECCCHHHHCEEEEECCCCCC
APVMVHRAICGSIERFIGILIEHFAGHFPLWLAPVQAVVTTITSDGDEYAKVVASAARRA
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GLRVELDLRNEKINYKVREHSLAKIPALLVVGKKEAETQSVSIRRLGSDGQKVTPTGEAI
CCEEEEEECCCCCCEEEHHHHHHHCCEEEEECCCCCCCHHHHHEECCCCCCEECCCHHHH
AALVAEAVPPDIQRIKTAPQSLSASD
HHHHHHHCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SENQDPAGFRYGLANLKPVSPADNVVLTFPDGATREFPKNTTGLEIAKGISPSLAKRTV
CCCCCCCCCEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHCCCCHHHHHHHH
AMALDGIVNDLADPIEQDATIEFISRDDPRALELIRHDAAHVLAEAVQTLWPGTQVTIGP
HHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
VIENGFYYDFFRNEPFSVEDFAAIEKKMREIIARDQPFTKDVWDREKTKQVFRDKGEAFK
EECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEE
VELVDAIPGNEPIRIYYQGDWFDLCRGPHMTSTGKIGNAFKLMKVAGAYWRGDSNNPMLT
EEEEECCCCCCCEEEEECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEE
RIYGTAFANQADLDAYLKQIEEAEKRDHRKLGRELDLFHFQEEGPGVVFWHAKGWTIFQA
EEECHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCHHHHHH
VIAYMRRRLAEDYDEVNAPQMLDKSLWETSGHWEWYRENMFAAQSAGDEAEDKRWFTIKP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEC
MNCPGHVQLFKHGLKSYRDLPLRLAEFGVVHRYEPSGAMHGLMRVRGFTQDDAHVFCTEE
CCCCCHHHHHHHHHHHHHCCCHHHHHHCCEEEECCCHHHHHHHHHCCCCCCCCEEEECHH
QLADECLKINELILSTYADFGFDGELTVKLSTRPEKRVGSDAAWDHAEAVMADVLKHIAA
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHHHHC
SNNRIRTETNPGEGAFYGPKFEYVLRDAIGRDWQCGTTQVDFNLPERFGAFYIDADGGKK
CCCEEEECCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEECCCHHHHCEEEEECCCCCC
APVMVHRAICGSIERFIGILIEHFAGHFPLWLAPVQAVVTTITSDGDEYAKVVASAARRA
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GLRVELDLRNEKINYKVREHSLAKIPALLVVGKKEAETQSVSIRRLGSDGQKVTPTGEAI
CCEEEEEECCCCCCEEEHHHHHHHCCEEEEECCCCCCCHHHHHEECCCCCCEECCCHHHH
AALVAEAVPPDIQRIKTAPQSLSASD
HHHHHHHCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA