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The map label for this gene is xthA [H]

Identifier: 75676002

GI number: 75676002

Start: 1983753

End: 1984553

Strand: Direct

Name: xthA [H]

Synonym: Nwi_1810

Alternate gene names: 75676002

Gene position: 1983753-1984553 (Clockwise)

Preceding gene: 75676001

Following gene: 75676015

Centisome position: 58.31

GC content: 61.8

Gene sequence:

>801_bases
ATGCGCATAGCCACCTGGAACGTCAATTCGGTCCGGCAGCGCCTGGATCACCTGATCACCTGGCTGAGAGAGACCGCGCC
GGACGTCGTCTGCCTTCAGGAGATCAAATGCGTCGATGAGCAGTTTCCCCGCGAAGCCATCGAGGCGCTCGGCTACAATG
TGGTGACGCACGGCCAGAAGACCTTCAACGGCGTGGCGCTGCTATCGAAATTCCCGTTCGACGAGACCCGGCCCCGGCTG
GCGGGCGATGACGGCGACGTGCAGGCGCGATTCCTCGAAGGCGTCGTCACCCTCGAGCATGGGGCGGTGCGCGTCGCCTG
CCTCTATCTGCCCAACGGCAACCCTCCGAACACAGAGAAATATCCCTACAAACTCAAGTGGATATCGCGCCTTATTGAGT
ATTCGAGGGAACGATTGAAAAGCGAGGAAGCCTTCATACTCGCCGGCGATTTCAACGTCATCCCCGCGCCGTGCGACGTC
TACAATTCCGCCGCATGGGTCGATGACGCCCTGTTCCGCCCGCGGACACGGCAAGCTTTCCAGACGCTGCTCGGGCTTGG
GCTGACCGACGCCCTACGCGCGGTGTCAGACGCCGCCGGAGAATATACGTTCTGGGACTATCAGGCGGGCGCCTGGCAGA
AGAACAACGGCTTGCGGATCGATCATCTGCTGCTGTCGCCGCAGGCCAGCGACCGGCTCAGCGGAGCGGGAATCGATCGT
CATGTGCGCGGCTGGGAAAAAGCGTCCGACCACGTCCCGGTCTGGATTGATCTCGATCTGGAAACGGCTGGAAGGAACTA
G

Upstream 100 bases:

>100_bases
TCGATATAGTTTTTCGGGGTCAGAGGCGGCCTTGACCCGCTGAAAGAGACCTCACGGCGGATTGCCCGTTGAGACCGGCA
ATCACATGCGGGACCGCCTG

Downstream 100 bases:

>100_bases
AGCTTTTCAGCGAAGGAGAAGCACCGGTTCGCTTGAAGAAAACGCGCCAAAACAACAATCTGGAGCCCTCGCTTCTGATT
ACGTCAGAAGTGAAAACCTC

Product: exodeoxyribonuclease III xth

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MRIATWNVNSVRQRLDHLITWLRETAPDVVCLQEIKCVDEQFPREAIEALGYNVVTHGQKTFNGVALLSKFPFDETRPRL
AGDDGDVQARFLEGVVTLEHGAVRVACLYLPNGNPPNTEKYPYKLKWISRLIEYSRERLKSEEAFILAGDFNVIPAPCDV
YNSAAWVDDALFRPRTRQAFQTLLGLGLTDALRAVSDAAGEYTFWDYQAGAWQKNNGLRIDHLLLSPQASDRLSGAGIDR
HVRGWEKASDHVPVWIDLDLETAGRN

Sequences:

>Translated_266_residues
MRIATWNVNSVRQRLDHLITWLRETAPDVVCLQEIKCVDEQFPREAIEALGYNVVTHGQKTFNGVALLSKFPFDETRPRL
AGDDGDVQARFLEGVVTLEHGAVRVACLYLPNGNPPNTEKYPYKLKWISRLIEYSRERLKSEEAFILAGDFNVIPAPCDV
YNSAAWVDDALFRPRTRQAFQTLLGLGLTDALRAVSDAAGEYTFWDYQAGAWQKNNGLRIDHLLLSPQASDRLSGAGIDR
HVRGWEKASDHVPVWIDLDLETAGRN
>Mature_266_residues
MRIATWNVNSVRQRLDHLITWLRETAPDVVCLQEIKCVDEQFPREAIEALGYNVVTHGQKTFNGVALLSKFPFDETRPRL
AGDDGDVQARFLEGVVTLEHGAVRVACLYLPNGNPPNTEKYPYKLKWISRLIEYSRERLKSEEAFILAGDFNVIPAPCDV
YNSAAWVDDALFRPRTRQAFQTLLGLGLTDALRAVSDAAGEYTFWDYQAGAWQKNNGLRIDHLLLSPQASDRLSGAGIDR
HVRGWEKASDHVPVWIDLDLETAGRN

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=267, Percent_Identity=29.5880149812734, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI18375503, Length=267, Percent_Identity=29.5880149812734, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI18375501, Length=267, Percent_Identity=29.5880149812734, Blast_Score=106, Evalue=2e-23,
Organism=Escherichia coli, GI1788046, Length=263, Percent_Identity=34.6007604562738, Blast_Score=146, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71989536, Length=270, Percent_Identity=28.1481481481481, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221330655, Length=268, Percent_Identity=28.3582089552239, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17136678, Length=268, Percent_Identity=28.3582089552239, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30022; Mature: 30022

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIATWNVNSVRQRLDHLITWLRETAPDVVCLQEIKCVDEQFPREAIEALGYNVVTHGQK
CEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHCCCCEEECCCH
TFNGVALLSKFPFDETRPRLAGDDGDVQARFLEGVVTLEHGAVRVACLYLPNGNPPNTEK
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCEEECCCEEEEEEEECCCCCCCCCC
YPYKLKWISRLIEYSRERLKSEEAFILAGDFNVIPAPCDVYNSAAWVDDALFRPRTRQAF
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCHHHCCCHHHHHHHHCCCHHHHHH
QTLLGLGLTDALRAVSDAAGEYTFWDYQAGAWQKNNGLRIDHLLLSPQASDRLSGAGIDR
HHHHHCCHHHHHHHHHHCCCCCEEEECCCCCEECCCCCEEEEEEECCCCCCCCCCCCCHH
HVRGWEKASDHVPVWIDLDLETAGRN
HHCCHHCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MRIATWNVNSVRQRLDHLITWLRETAPDVVCLQEIKCVDEQFPREAIEALGYNVVTHGQK
CEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHCCCCEEECCCH
TFNGVALLSKFPFDETRPRLAGDDGDVQARFLEGVVTLEHGAVRVACLYLPNGNPPNTEK
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCEEECCCEEEEEEEECCCCCCCCCC
YPYKLKWISRLIEYSRERLKSEEAFILAGDFNVIPAPCDVYNSAAWVDDALFRPRTRQAF
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCHHHCCCHHHHHHHHCCCHHHHHH
QTLLGLGLTDALRAVSDAAGEYTFWDYQAGAWQKNNGLRIDHLLLSPQASDRLSGAGIDR
HHHHHCCHHHHHHHHHHCCCCCEEEECCCCCEECCCCCEEEEEEECCCCCCCCCCCCCHH
HVRGWEKASDHVPVWIDLDLETAGRN
HHCCHHCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]