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The map label for this gene is dacF [H]

Identifier: 75675891

GI number: 75675891

Start: 1852951

End: 1854714

Strand: Direct

Name: dacF [H]

Synonym: Nwi_1699

Alternate gene names: 75675891

Gene position: 1852951-1854714 (Clockwise)

Preceding gene: 75675889

Following gene: 75675894

Centisome position: 54.47

GC content: 65.7

Gene sequence:

>1764_bases
ATGCTTCGCACCACCTTGGCTTCCTCGCGCGCAACGCGTGTTGGCGCGCTTGGGCTTGCAGCGATCATCGCCGTCGTTTT
TACGATCGATCATGCAGATGCGCGGCGTGCCCGTCAGCACCATCGCCACCACCAGCAGCAGGTGCGGCATAGCTACAATC
CGCCCTTCGCGTCGATCGTGGTGGATGCTAATTCCGGGGCGACGCTGTCGTCCACCAGCCCCGACAGTCTTCGCCATCCG
GCGTCGCTCACCAAGATGATGACGCTCTACATGCTTTTCGAGCGTCTGGACGCCGGCAGGATCGGCCTCGACAGCGAGAT
GCGCGTTTCAGCGCGCGCGGCCGCGCAGAACCCGACCAAGCTCGGCCTGCGACCGGGTCAGACCATCCGCGTCGAAGATG
CGATCAAGGGGCTGGTGACGAAATCCGCCAACGACGCGGCGGTCGTCATCGCCGAGGCGATCGCAGGCGACGAAGACGAC
TTCGCCAGGATGATGACGCGGAAGGCCCGTGCGCTCGGCATGACACGGACGGTCTATCGCAACGCTTCAGGTCTTCCCGA
CGACGATCAAGTCACCACGGCGCGGGACCAGGCAACGCTCGGCCGTGCGTTGCAGGATCGTTTCCCGCGCTACTATCGCT
ATTTCGCGACCACCTCATTCACGTTCCGTGGTCGCACCATTCGGGGTCATAACCGTCTGGTCGGCAGCGTCGATGGCGTC
GACGGTATCAAAACAGGCTATATTCGAGCTTCCGGCTTCAATCTCGTCACCAACCTCAAGCGCGGCAATCGCCACCTTGT
CGGCGTGGTTCTTGGCGGTCGCAGCGGCGGCTCCCGCGACGCCACCATGCGCAAACTGCTGGCGGAAAATCTCGACAAGG
CCGCGACGCGGCGGACGGTCGCCGCGATTGCCGAAAGCAGCATGGCGGAGGCCAATGCGAGGGCGGCCGAGGATCAGGCG
CGCTCACGGCCGAGGGGTACGACGCAGGTGGACGGAGCCGTTCAAGTCGCGTCCACCGTGCCCGAGCTTGTCTCGCTGCC
GGCCGAGCGTCCCGCTCATCTGAGCCGATCGCTGACAGCGGCTCCGGCGGCGGCGTTACCTCCGAAGCGGCCTTTAGCCG
AACCGGCTCCCTTGACGAGCGGCGTTATCGAAACCCAGCCGATCGACGCCGTTCCCGGCTCGGCCGAACCGATGAAGCCG
GTGCGGGTCAAGACGGTACAGGTCAGGGCGAAAGCGGTCAGACTGGCATCGGCCGGGCCGGCGCCTGCTTTGTCTCCGGC
CGCCGGCGACGCCGCAGCGGATCGCGAGGCGGCGCCGGAAACGTCGGGCTCGGTCGTGGCGCGGGCGAACATGCCGCCGC
AGCCTGCCAACTTCGGCACCGGTCAGGGCATCCTGGGGGTGCTTCCGGCCTCCAGTCTTCCTGCTTCGACAAGGCAGGCG
ATGGTTCATGCTGATCCGGCGCCCGCGGTTCAGCCGCGAACAGTTCAGAAGAATGGCGCGCTCAAGCCGGTCGCGTCGCA
TTCGGGTTGGATCATTCAGGTCGGCGCGCTGGAAACCGAGAGCCAGGCGCGCGAGCGTCTCGATGCGGCCCGAGGCCGCG
CTCCGAGGATGCTGGGCAAGGCCGATCCTTTCACCGAGCCCGTCGCCAAGGGCAGCAAGAAGCTATATCGCGCCCGCTTC
GCCGGCCTCGAACGCGATGAGGCCGAAGCCGTGTGCCGCAAGCTGAAGCGTTCGGATATATCCTGCATCACGATCAAAAA
CTGA

Upstream 100 bases:

>100_bases
CGAGAAAGCCGGTTCGCGCCGAACGCTCAGATCAGATGGCCGGGCAAAATTTTGCCTCACGAACCAGTGACTAAAAGTCG
AAAATAGGACGGGGAAGTAT

Downstream 100 bases:

>100_bases
TCGCCCGCCCCGCACTCATACGCGACGCCAGAATCCAGAGCTTTATGCTTCTGATGCAATATCAGAAGCATGGCTCTCGA
ATTTGACGCGTTTTGCTTTG

Product: peptidase S11, D-alanyl-D-alanine carboxypeptidase 1

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MLRTTLASSRATRVGALGLAAIIAVVFTIDHADARRARQHHRHHQQQVRHSYNPPFASIVVDANSGATLSSTSPDSLRHP
ASLTKMMTLYMLFERLDAGRIGLDSEMRVSARAAAQNPTKLGLRPGQTIRVEDAIKGLVTKSANDAAVVIAEAIAGDEDD
FARMMTRKARALGMTRTVYRNASGLPDDDQVTTARDQATLGRALQDRFPRYYRYFATTSFTFRGRTIRGHNRLVGSVDGV
DGIKTGYIRASGFNLVTNLKRGNRHLVGVVLGGRSGGSRDATMRKLLAENLDKAATRRTVAAIAESSMAEANARAAEDQA
RSRPRGTTQVDGAVQVASTVPELVSLPAERPAHLSRSLTAAPAAALPPKRPLAEPAPLTSGVIETQPIDAVPGSAEPMKP
VRVKTVQVRAKAVRLASAGPAPALSPAAGDAAADREAAPETSGSVVARANMPPQPANFGTGQGILGVLPASSLPASTRQA
MVHADPAPAVQPRTVQKNGALKPVASHSGWIIQVGALETESQARERLDAARGRAPRMLGKADPFTEPVAKGSKKLYRARF
AGLERDEAEAVCRKLKRSDISCITIKN

Sequences:

>Translated_587_residues
MLRTTLASSRATRVGALGLAAIIAVVFTIDHADARRARQHHRHHQQQVRHSYNPPFASIVVDANSGATLSSTSPDSLRHP
ASLTKMMTLYMLFERLDAGRIGLDSEMRVSARAAAQNPTKLGLRPGQTIRVEDAIKGLVTKSANDAAVVIAEAIAGDEDD
FARMMTRKARALGMTRTVYRNASGLPDDDQVTTARDQATLGRALQDRFPRYYRYFATTSFTFRGRTIRGHNRLVGSVDGV
DGIKTGYIRASGFNLVTNLKRGNRHLVGVVLGGRSGGSRDATMRKLLAENLDKAATRRTVAAIAESSMAEANARAAEDQA
RSRPRGTTQVDGAVQVASTVPELVSLPAERPAHLSRSLTAAPAAALPPKRPLAEPAPLTSGVIETQPIDAVPGSAEPMKP
VRVKTVQVRAKAVRLASAGPAPALSPAAGDAAADREAAPETSGSVVARANMPPQPANFGTGQGILGVLPASSLPASTRQA
MVHADPAPAVQPRTVQKNGALKPVASHSGWIIQVGALETESQARERLDAARGRAPRMLGKADPFTEPVAKGSKKLYRARF
AGLERDEAEAVCRKLKRSDISCITIKN
>Mature_587_residues
MLRTTLASSRATRVGALGLAAIIAVVFTIDHADARRARQHHRHHQQQVRHSYNPPFASIVVDANSGATLSSTSPDSLRHP
ASLTKMMTLYMLFERLDAGRIGLDSEMRVSARAAAQNPTKLGLRPGQTIRVEDAIKGLVTKSANDAAVVIAEAIAGDEDD
FARMMTRKARALGMTRTVYRNASGLPDDDQVTTARDQATLGRALQDRFPRYYRYFATTSFTFRGRTIRGHNRLVGSVDGV
DGIKTGYIRASGFNLVTNLKRGNRHLVGVVLGGRSGGSRDATMRKLLAENLDKAATRRTVAAIAESSMAEANARAAEDQA
RSRPRGTTQVDGAVQVASTVPELVSLPAERPAHLSRSLTAAPAAALPPKRPLAEPAPLTSGVIETQPIDAVPGSAEPMKP
VRVKTVQVRAKAVRLASAGPAPALSPAAGDAAADREAAPETSGSVVARANMPPQPANFGTGQGILGVLPASSLPASTRQA
MVHADPAPAVQPRTVQKNGALKPVASHSGWIIQVGALETESQARERLDAARGRAPRMLGKADPFTEPVAKGSKKLYRARF
AGLERDEAEAVCRKLKRSDISCITIKN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=240, Percent_Identity=37.5, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1787062, Length=238, Percent_Identity=36.9747899159664, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI87082022, Length=238, Percent_Identity=34.8739495798319, Blast_Score=133, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 62379; Mature: 62379

Theoretical pI: Translated: 11.25; Mature: 11.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRTTLASSRATRVGALGLAAIIAVVFTIDHADARRARQHHRHHQQQVRHSYNPPFASIV
CCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
VDANSGATLSSTSPDSLRHPASLTKMMTLYMLFERLDAGRIGLDSEMRVSARAAAQNPTK
EECCCCCEECCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCC
LGLRPGQTIRVEDAIKGLVTKSANDAAVVIAEAIAGDEDDFARMMTRKARALGMTRTVYR
CCCCCCCEEEHHHHHHHHHHCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHCHHHHHHH
NASGLPDDDQVTTARDQATLGRALQDRFPRYYRYFATTSFTFRGRTIRGHNRLVGSVDGV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCEECCCCCEEECCCCC
DGIKTGYIRASGFNLVTNLKRGNRHLVGVVLGGRSGGSRDATMRKLLAENLDKAATRRTV
CCCCCCEEEECCCHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
AAIAESSMAEANARAAEDQARSRPRGTTQVDGAVQVASTVPELVSLPAERPAHLSRSLTA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHC
APAAALPPKRPLAEPAPLTSGVIETQPIDAVPGSAEPMKPVRVKTVQVRAKAVRLASAGP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHCCCC
APALSPAAGDAAADREAAPETSGSVVARANMPPQPANFGTGQGILGVLPASSLPASTRQA
CCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHHHHH
MVHADPAPAVQPRTVQKNGALKPVASHSGWIIQVGALETESQARERLDAARGRAPRMLGK
EEECCCCCCCCCCEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCC
ADPFTEPVAKGSKKLYRARFAGLERDEAEAVCRKLKRSDISCITIKN
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MLRTTLASSRATRVGALGLAAIIAVVFTIDHADARRARQHHRHHQQQVRHSYNPPFASIV
CCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
VDANSGATLSSTSPDSLRHPASLTKMMTLYMLFERLDAGRIGLDSEMRVSARAAAQNPTK
EECCCCCEECCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCC
LGLRPGQTIRVEDAIKGLVTKSANDAAVVIAEAIAGDEDDFARMMTRKARALGMTRTVYR
CCCCCCCEEEHHHHHHHHHHCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHCHHHHHHH
NASGLPDDDQVTTARDQATLGRALQDRFPRYYRYFATTSFTFRGRTIRGHNRLVGSVDGV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCEECCCCCEEECCCCC
DGIKTGYIRASGFNLVTNLKRGNRHLVGVVLGGRSGGSRDATMRKLLAENLDKAATRRTV
CCCCCCEEEECCCHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
AAIAESSMAEANARAAEDQARSRPRGTTQVDGAVQVASTVPELVSLPAERPAHLSRSLTA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHC
APAAALPPKRPLAEPAPLTSGVIETQPIDAVPGSAEPMKPVRVKTVQVRAKAVRLASAGP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHCCCC
APALSPAAGDAAADREAAPETSGSVVARANMPPQPANFGTGQGILGVLPASSLPASTRQA
CCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHHHHH
MVHADPAPAVQPRTVQKNGALKPVASHSGWIIQVGALETESQARERLDAARGRAPRMLGK
EEECCCCCCCCCCEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCC
ADPFTEPVAKGSKKLYRARFAGLERDEAEAVCRKLKRSDISCITIKN
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]