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The map label for this gene is phbC [H]

Identifier: 75675842

GI number: 75675842

Start: 1793436

End: 1795265

Strand: Direct

Name: phbC [H]

Synonym: Nwi_1650

Alternate gene names: 75675842

Gene position: 1793436-1795265 (Clockwise)

Preceding gene: 75675836

Following gene: 75675845

Centisome position: 52.72

GC content: 62.24

Gene sequence:

>1830_bases
ATGAGCAAGCTCCAAGACGCGTCCATGAGCGAGGTATCCGCCGAGGCCAAGCCGACGAAGGGGTTCGACTCCGACGCATT
CGCGATGAATCTCGCGAGTGCTATGGAGAACGGCGGCAGGGCGCTGGCCGCGTATCTCAAGGCCCGGGAGCAGAAGCAGA
CCCCCGACAACCCGCCTACCGAGTTTGCCGAACTGATCAAGACCGTCAGCGCGGTCTCCGAATACTGGCTGTCGGACCAG
GCCAGGGCCGTCGATTTACAGGCCCGGCTTGGCAAGTCCTATCTGGAACTCTGGGGTTACGCTGCGCGCCGCCTTGCCGG
CGAGGAGGTCGAGCCAGCGATCGAACCCGATCCGCGCGACAAGCGCTTCGCCGACCCGGAATGGCGGTCGAACCTGCTGT
TCAACTTCCTGATGCAGGCGTATCTGCTGACGACCAAATGGACCAACGAACTGGTGCGCGACGCCCACGGCCTCGATCCC
CATACCCGCAAGAAGGCTGAGTTCTATATCACGCAGCTCACCAACGCGGCCGCGCCGTCCAATTTCGTGATGACCAACCC
CGAAGTGCTCCGCGAGACCTTCGCCTCCAACGCCGACAATCTGGCGCGCGGCATGAAGATGCTGGCGGAAGACATCGAGG
CTGGCCGGGGCGTGTTGCGCATCCGCCAATCCGACTCCGCTGGACTCGCCGTCGGCGTCAACCTGGCGACGACGCCCGGT
AAGGTGATCTACCAGAACGAACTGATGCAATTGATCCAGTATTCACCGAGCACCGAAAACGTGCTGCGGACGCCGCTTTT
GATCGTGCCGCCATGGATCAACAAATTCTACATCCTCGATCTCACGCCGGAGAAGTCCTACATCAGGTGGTGCGTGGATC
AGGGCATCACCGTGTTCGTGATCTCCTGGGTCAACCCGGACGGACATCTCGGGGAAAAGACCTTCGAGGACTACATGAAG
CAAGGTCCGCTGGCCGCGATGGACGCCATCGAAAAGGCCACCGGAGAAACGAAGGTCCATACCGCGGGTTACTGCGTCGG
CGGCACCCTGCTTGCCGCCACGCTGGCATGGCTTGCGAACAAGCGGCGGCAGCGCGTGCGGTCGGTGACCTTCTTCGCCG
CTCAGGTGGACTTCACCCATGCCGGCGATCTGCTGGTATTCGCCGATGAGGATCAGATCTCCGCGCTCGAACGCGACATG
AAGCGGTCCGGCGTGCTGGAAGGCAGCAAGATGGCGATGACCTTCAACATGCTGCGCTCCAACGACCTGATCTGGCCCTA
CGTCGTCAGCAATTATCTCAAGGGCAAGGCGCCATCCGCCTTCGATCTCCTGCACTGGAATTCAGACGCCACGCGGATGC
CGGCCGCCAACCACTCGTTTTATCTGCGCAACTGCTATCTCGAGAACCGCCTTTCAACCGGCTCGATGGTGCTCGACAAT
ACCTTGCTCGACCTTTCCAGGGTCAGGGTGCCGGTCTACAATCTCGCGACCCGCGACGATCACATCGCGCCGGCGGAATC
CGTGTTGTACGGCTCGCGTTTTTTTGGCGGGCCGGTGCGCTTCGTTCTGGCGGGATCGGGCCACATCGCAGGTGTGATCA
ATCCGCCCTCGCGCGGCAAATACCAGTATTGGACCAACGACGGGATCAGCGACAAAACCCTCGCCCAGTGGATGCAGGAC
GCGCAGGAGCACAAGGGCTCGTGGTGGCCGGACTGGCTCGCATGGCTGCGAAACATCGACGGCAAGGAGGTTCCGGCGCG
CGAGGTCGGAACCGGCGCGCTGTCGCCGATCGAGGACGCGCCGGGCAGCTATGTGCGCGTGCGTGCTTGA

Upstream 100 bases:

>100_bases
ATCAAGGAGTATTGTCGCACTGCACCACATCGAATCGGGCAATGATCCCCAGATTATGTCATGATCGATGCCCGCCAATG
GCAACCTACGTGACGGAACG

Downstream 100 bases:

>100_bases
GATATAGCGCTTTCGAACGAAGATGACCTGATCGCGTGAAGGCAACGTGTCATGGCCGGCACATAAGCCGGGCGTGATGA
TTCAATCAAACCTCATTCCA

Product: Poly(R)-hydroxyalkanoic acid synthase, class I

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 609; Mature: 608

Protein sequence:

>609_residues
MSKLQDASMSEVSAEAKPTKGFDSDAFAMNLASAMENGGRALAAYLKAREQKQTPDNPPTEFAELIKTVSAVSEYWLSDQ
ARAVDLQARLGKSYLELWGYAARRLAGEEVEPAIEPDPRDKRFADPEWRSNLLFNFLMQAYLLTTKWTNELVRDAHGLDP
HTRKKAEFYITQLTNAAAPSNFVMTNPEVLRETFASNADNLARGMKMLAEDIEAGRGVLRIRQSDSAGLAVGVNLATTPG
KVIYQNELMQLIQYSPSTENVLRTPLLIVPPWINKFYILDLTPEKSYIRWCVDQGITVFVISWVNPDGHLGEKTFEDYMK
QGPLAAMDAIEKATGETKVHTAGYCVGGTLLAATLAWLANKRRQRVRSVTFFAAQVDFTHAGDLLVFADEDQISALERDM
KRSGVLEGSKMAMTFNMLRSNDLIWPYVVSNYLKGKAPSAFDLLHWNSDATRMPAANHSFYLRNCYLENRLSTGSMVLDN
TLLDLSRVRVPVYNLATRDDHIAPAESVLYGSRFFGGPVRFVLAGSGHIAGVINPPSRGKYQYWTNDGISDKTLAQWMQD
AQEHKGSWWPDWLAWLRNIDGKEVPAREVGTGALSPIEDAPGSYVRVRA

Sequences:

>Translated_609_residues
MSKLQDASMSEVSAEAKPTKGFDSDAFAMNLASAMENGGRALAAYLKAREQKQTPDNPPTEFAELIKTVSAVSEYWLSDQ
ARAVDLQARLGKSYLELWGYAARRLAGEEVEPAIEPDPRDKRFADPEWRSNLLFNFLMQAYLLTTKWTNELVRDAHGLDP
HTRKKAEFYITQLTNAAAPSNFVMTNPEVLRETFASNADNLARGMKMLAEDIEAGRGVLRIRQSDSAGLAVGVNLATTPG
KVIYQNELMQLIQYSPSTENVLRTPLLIVPPWINKFYILDLTPEKSYIRWCVDQGITVFVISWVNPDGHLGEKTFEDYMK
QGPLAAMDAIEKATGETKVHTAGYCVGGTLLAATLAWLANKRRQRVRSVTFFAAQVDFTHAGDLLVFADEDQISALERDM
KRSGVLEGSKMAMTFNMLRSNDLIWPYVVSNYLKGKAPSAFDLLHWNSDATRMPAANHSFYLRNCYLENRLSTGSMVLDN
TLLDLSRVRVPVYNLATRDDHIAPAESVLYGSRFFGGPVRFVLAGSGHIAGVINPPSRGKYQYWTNDGISDKTLAQWMQD
AQEHKGSWWPDWLAWLRNIDGKEVPAREVGTGALSPIEDAPGSYVRVRA
>Mature_608_residues
SKLQDASMSEVSAEAKPTKGFDSDAFAMNLASAMENGGRALAAYLKAREQKQTPDNPPTEFAELIKTVSAVSEYWLSDQA
RAVDLQARLGKSYLELWGYAARRLAGEEVEPAIEPDPRDKRFADPEWRSNLLFNFLMQAYLLTTKWTNELVRDAHGLDPH
TRKKAEFYITQLTNAAAPSNFVMTNPEVLRETFASNADNLARGMKMLAEDIEAGRGVLRIRQSDSAGLAVGVNLATTPGK
VIYQNELMQLIQYSPSTENVLRTPLLIVPPWINKFYILDLTPEKSYIRWCVDQGITVFVISWVNPDGHLGEKTFEDYMKQ
GPLAAMDAIEKATGETKVHTAGYCVGGTLLAATLAWLANKRRQRVRSVTFFAAQVDFTHAGDLLVFADEDQISALERDMK
RSGVLEGSKMAMTFNMLRSNDLIWPYVVSNYLKGKAPSAFDLLHWNSDATRMPAANHSFYLRNCYLENRLSTGSMVLDNT
LLDLSRVRVPVYNLATRDDHIAPAESVLYGSRFFGGPVRFVLAGSGHIAGVINPPSRGKYQYWTNDGISDKTLAQWMQDA
QEHKGSWWPDWLAWLRNIDGKEVPAREVGTGALSPIEDAPGSYVRVRA

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 67968; Mature: 67837

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLQDASMSEVSAEAKPTKGFDSDAFAMNLASAMENGGRALAAYLKAREQKQTPDNPPT
CCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHH
EFAELIKTVSAVSEYWLSDQARAVDLQARLGKSYLELWGYAARRLAGEEVEPAIEPDPRD
HHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
KRFADPEWRSNLLFNFLMQAYLLTTKWTNELVRDAHGLDPHTRKKAEFYITQLTNAAAPS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEHHHCCCCCC
NFVMTNPEVLRETFASNADNLARGMKMLAEDIEAGRGVLRIRQSDSAGLAVGVNLATTPG
CEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEECCCC
KVIYQNELMQLIQYSPSTENVLRTPLLIVPPWINKFYILDLTPEKSYIRWCVDQGITVFV
CEEEHHHHHHHHHCCCCCHHHHHCCEEEECCCCCCEEEEEECCCHHHHHHHHHCCCEEEE
ISWVNPDGHLGEKTFEDYMKQGPLAAMDAIEKATGETKVHTAGYCVGGTLLAATLAWLAN
EEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCEEHHHHHHHHHHHHHHH
KRRQRVRSVTFFAAQVDFTHAGDLLVFADEDQISALERDMKRSGVLEGSKMAMTFNMLRS
HHHHHHHHHHEEEEEEEEECCCCEEEEECCHHHHHHHHHHHHHCCCCCCHHEEEEEHHHC
NDLIWPYVVSNYLKGKAPSAFDLLHWNSDATRMPAANHSFYLRNCYLENRLSTGSMVLDN
CCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEHHHCCCCCCCEEHHH
TLLDLSRVRVPVYNLATRDDHIAPAESVLYGSRFFGGPVRFVLAGSGHIAGVINPPSRGK
HHHHHHHHCCCCEEECCCCCCCCCHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCC
YQYWTNDGISDKTLAQWMQDAQEHKGSWWPDWLAWLRNIDGKEVPAREVGTGALSPIEDA
EEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCC
PGSYVRVRA
CCCEEEEEC
>Mature Secondary Structure 
SKLQDASMSEVSAEAKPTKGFDSDAFAMNLASAMENGGRALAAYLKAREQKQTPDNPPT
CCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHH
EFAELIKTVSAVSEYWLSDQARAVDLQARLGKSYLELWGYAARRLAGEEVEPAIEPDPRD
HHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
KRFADPEWRSNLLFNFLMQAYLLTTKWTNELVRDAHGLDPHTRKKAEFYITQLTNAAAPS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEHHHCCCCCC
NFVMTNPEVLRETFASNADNLARGMKMLAEDIEAGRGVLRIRQSDSAGLAVGVNLATTPG
CEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEECCCC
KVIYQNELMQLIQYSPSTENVLRTPLLIVPPWINKFYILDLTPEKSYIRWCVDQGITVFV
CEEEHHHHHHHHHCCCCCHHHHHCCEEEECCCCCCEEEEEECCCHHHHHHHHHCCCEEEE
ISWVNPDGHLGEKTFEDYMKQGPLAAMDAIEKATGETKVHTAGYCVGGTLLAATLAWLAN
EEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCEEHHHHHHHHHHHHHHH
KRRQRVRSVTFFAAQVDFTHAGDLLVFADEDQISALERDMKRSGVLEGSKMAMTFNMLRS
HHHHHHHHHHEEEEEEEEECCCCEEEEECCHHHHHHHHHHHHHCCCCCCHHEEEEEHHHC
NDLIWPYVVSNYLKGKAPSAFDLLHWNSDATRMPAANHSFYLRNCYLENRLSTGSMVLDN
CCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEHHHCCCCCCCEEHHH
TLLDLSRVRVPVYNLATRDDHIAPAESVLYGSRFFGGPVRFVLAGSGHIAGVINPPSRGK
HHHHHHHHCCCCEEECCCCCCCCCHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCC
YQYWTNDGISDKTLAQWMQDAQEHKGSWWPDWLAWLRNIDGKEVPAREVGTGALSPIEDA
EEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCC
PGSYVRVRA
CCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748438 [H]