Click here to switch to the map view.

The map label for this gene is gyrB [H]

Identifier: 75675845

GI number: 75675845

Start: 1797177

End: 1799234

Strand: Direct

Name: gyrB [H]

Synonym: Nwi_1653

Alternate gene names: 75675845

Gene position: 1797177-1799234 (Clockwise)

Preceding gene: 75675842

Following gene: 75675846

Centisome position: 52.83

GC content: 64.72

Gene sequence:

>2058_bases
ATGGTCAAATCACTGAAATCGAACATTAAAAAGAAACAAGCTGACGACCTTTTCGCGGCTTCGGAAACCAAGGGCCGGTC
CTCCGCGAGGGCCCCAGGCCGGGCATCGGGAGCCGAGGCGGACTATACCGCCGCCGACATCGAGGTGCTGGAGGGACTTG
AGCCGGTCCGCCGCCGCCCCGGAATGTATATCGGCGGCACCGACGAGAAGGCGCTGCATCATCTGTTCGCCGAGGTGATC
GACAACGCCATGGACGAGGCGCTGGCCGGCCACGCCACCTTCATCGTGGTGGAGCTTGCCGCTGACGGCTTCCTCACCGT
CACCGACAACGGGCGCGGCATCCCGGTCGATCCGCACCCGAAATTCCCGAAGAAATCGGCGCTTGAAGTCATCATGTGCA
CGCTGCATTCGGGCGGCAAGTTCGACTCCAAGGTGTATGAAACCTCCGGCGGCCTGCACGGCGTCGGCGTTTCCGTGGTC
AACGCCCTCTCCTCGCACCTCGAAGTGGAAGTGGCGCGCAGCCAGAACCTCTACAGGATGACCTTCGAGCGCGGCCACCC
CAAGGGCAAGCTCGAGGACCTCGGCAGGATAGCCAACCGCCGCGGCACCACCATTCGTTTCAAGCCGGACACTGAAATCT
TCGGGGCGAAGGCCGCGTTCAAGCCGCAGCGCCTGTTCAAGATGACGCGCTCGAAGGCCTACCTGTTCGGCGGCGTCGAA
ATCCGCTGGCGGTGCGCGCCGGAACTGCTCAAGGGCCTCGAGGATCTTCCTCCGGAAGCGACGTTCCACTTCCCCGGCGG
CCTGAAAGACTACCTTGCCGCGGCGATCCACAAGGACACGCTGGTCCATCCGGATATCTTCTCTGGCAGATCGGGCCGCA
ACGGCGCGCACGGCGCCTGCGAATGGGCGGTGGCATGGACCGCGGATGCCGACGGATTCCTGTCCTCCTACTGCAACACG
GTGCCGACGCCCGACGGCGGCACCCATGAGTCGGGCTTGCGCAGCGCGCTGCTGCGCGGGCTGAAGGATCATGCCGAGCG
CGTCGGCCAGGGCAAGCGCGCGGGCAGCATCACGTCGGAGGATGTGATGGTGGGCGCCGCCGTGATGCTCAGCGTGTTCG
TGCGCGAGCCTGAGTTTCAGGGCCAGACCAAGGACCGCCTCGCCACCGCCGAGGCGTCGCGGATCGTCGAGCAGGCGATC
AAGGATCCGTTCGATCATTGGCTGTCCGGCAATCCGGCGCAGGCCAACAGGCTGCTCGACTTCGTGATCGAGCGCGCGGA
CGAGCGACTGCGCCGCCGCGCCGAGAAGGAAACCTCGCGCAAGAGCGCCGTGAAGAAGCTTCGGCTGCCCGGCAAGCTTG
CCGACTGCACCAACACCGCGACGGAAGGCTCGGAGCTGTTCATCGTCGAGGGCGACTCGGCCGGCGGCAGCGCCAAGCAG
GCGCGCGATCGCAAGACCCAGGCCGTGCTGCCTTTGCGCGGCAAAATCCTCAACGTCGTATCCGCGACGCGGGACAAGGT
CACAGCCAACGCCCAGTTGGCGGATCTCATCCAGGCGCTGGGCTGCGGCACCGGGACCAACTACCGCGAGGAGGATCTGC
GCTATTCACGCGTCATCATCATGACCGACGCCGATGTCGATGGCGCCCATATCGCCTCGCTGCTGATTACGTTCTTCTAC
CGGCAGATGCCTCGCCTGATCGACGAGGGTCACCTCTACCTCGCGGTACCCCCGCTTTATCGCCTCACCCACGGCAGCAA
GACGGTCTACGCCCGCGACGAGGCCCACAAGGATGCGCTGCTCAAGAGCGAGTTCAACGCCAACGCCAAGGTCGATGTCG
GCCGGTTCAAAGGGCTGGGCGAGATGATGCCGGCGCAGCTCAAGGAAACCACGATGGATCCCGCCAAGCGCACGATGCTG
CGGGTCATGCTGCTTGCGGAAGATCGCGAGGACACGGCGGATTCAGTCGAGCGGTTGATGGGCACCAAGGCGGAGGCGCG
CTTCGCCTTCATTTCGGACAAGGCTGTATTCGCCAGCGAGGACATGCTGGACGTTTAA

Upstream 100 bases:

>100_bases
AGACTTCAAGTCACAAAGGCGCGGACGGCACGAAACCAGCGTGGCATAGTCGGGGCAGCCGATTCGTTCGCACGCAGCTT
TATGCAATATCTAAGTAATT

Downstream 100 bases:

>100_bases
GCCCTCTCCTGTCCCAAGCCCTCTCCTTCCCAAGGGTGCGCCTATCTGAAGCATGGGCCAAGTAGTTGACTGGATTCCGG
TGCCAAGTTGCGGTCATAAT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 685; Mature: 685

Protein sequence:

>685_residues
MVKSLKSNIKKKQADDLFAASETKGRSSARAPGRASGAEADYTAADIEVLEGLEPVRRRPGMYIGGTDEKALHHLFAEVI
DNAMDEALAGHATFIVVELAADGFLTVTDNGRGIPVDPHPKFPKKSALEVIMCTLHSGGKFDSKVYETSGGLHGVGVSVV
NALSSHLEVEVARSQNLYRMTFERGHPKGKLEDLGRIANRRGTTIRFKPDTEIFGAKAAFKPQRLFKMTRSKAYLFGGVE
IRWRCAPELLKGLEDLPPEATFHFPGGLKDYLAAAIHKDTLVHPDIFSGRSGRNGAHGACEWAVAWTADADGFLSSYCNT
VPTPDGGTHESGLRSALLRGLKDHAERVGQGKRAGSITSEDVMVGAAVMLSVFVREPEFQGQTKDRLATAEASRIVEQAI
KDPFDHWLSGNPAQANRLLDFVIERADERLRRRAEKETSRKSAVKKLRLPGKLADCTNTATEGSELFIVEGDSAGGSAKQ
ARDRKTQAVLPLRGKILNVVSATRDKVTANAQLADLIQALGCGTGTNYREEDLRYSRVIIMTDADVDGAHIASLLITFFY
RQMPRLIDEGHLYLAVPPLYRLTHGSKTVYARDEAHKDALLKSEFNANAKVDVGRFKGLGEMMPAQLKETTMDPAKRTML
RVMLLAEDREDTADSVERLMGTKAEARFAFISDKAVFASEDMLDV

Sequences:

>Translated_685_residues
MVKSLKSNIKKKQADDLFAASETKGRSSARAPGRASGAEADYTAADIEVLEGLEPVRRRPGMYIGGTDEKALHHLFAEVI
DNAMDEALAGHATFIVVELAADGFLTVTDNGRGIPVDPHPKFPKKSALEVIMCTLHSGGKFDSKVYETSGGLHGVGVSVV
NALSSHLEVEVARSQNLYRMTFERGHPKGKLEDLGRIANRRGTTIRFKPDTEIFGAKAAFKPQRLFKMTRSKAYLFGGVE
IRWRCAPELLKGLEDLPPEATFHFPGGLKDYLAAAIHKDTLVHPDIFSGRSGRNGAHGACEWAVAWTADADGFLSSYCNT
VPTPDGGTHESGLRSALLRGLKDHAERVGQGKRAGSITSEDVMVGAAVMLSVFVREPEFQGQTKDRLATAEASRIVEQAI
KDPFDHWLSGNPAQANRLLDFVIERADERLRRRAEKETSRKSAVKKLRLPGKLADCTNTATEGSELFIVEGDSAGGSAKQ
ARDRKTQAVLPLRGKILNVVSATRDKVTANAQLADLIQALGCGTGTNYREEDLRYSRVIIMTDADVDGAHIASLLITFFY
RQMPRLIDEGHLYLAVPPLYRLTHGSKTVYARDEAHKDALLKSEFNANAKVDVGRFKGLGEMMPAQLKETTMDPAKRTML
RVMLLAEDREDTADSVERLMGTKAEARFAFISDKAVFASEDMLDV
>Mature_685_residues
MVKSLKSNIKKKQADDLFAASETKGRSSARAPGRASGAEADYTAADIEVLEGLEPVRRRPGMYIGGTDEKALHHLFAEVI
DNAMDEALAGHATFIVVELAADGFLTVTDNGRGIPVDPHPKFPKKSALEVIMCTLHSGGKFDSKVYETSGGLHGVGVSVV
NALSSHLEVEVARSQNLYRMTFERGHPKGKLEDLGRIANRRGTTIRFKPDTEIFGAKAAFKPQRLFKMTRSKAYLFGGVE
IRWRCAPELLKGLEDLPPEATFHFPGGLKDYLAAAIHKDTLVHPDIFSGRSGRNGAHGACEWAVAWTADADGFLSSYCNT
VPTPDGGTHESGLRSALLRGLKDHAERVGQGKRAGSITSEDVMVGAAVMLSVFVREPEFQGQTKDRLATAEASRIVEQAI
KDPFDHWLSGNPAQANRLLDFVIERADERLRRRAEKETSRKSAVKKLRLPGKLADCTNTATEGSELFIVEGDSAGGSAKQ
ARDRKTQAVLPLRGKILNVVSATRDKVTANAQLADLIQALGCGTGTNYREEDLRYSRVIIMTDADVDGAHIASLLITFFY
RQMPRLIDEGHLYLAVPPLYRLTHGSKTVYARDEAHKDALLKSEFNANAKVDVGRFKGLGEMMPAQLKETTMDPAKRTML
RVMLLAEDREDTADSVERLMGTKAEARFAFISDKAVFASEDMLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=633, Percent_Identity=24.8025276461295, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI19913408, Length=636, Percent_Identity=26.1006289308176, Blast_Score=142, Evalue=1e-33,
Organism=Escherichia coli, GI1789408, Length=640, Percent_Identity=42.34375, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI48994957, Length=564, Percent_Identity=45.3900709219858, Blast_Score=451, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17535065, Length=608, Percent_Identity=23.5197368421053, Blast_Score=119, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI212645845, Length=559, Percent_Identity=23.2558139534884, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI212645657, Length=211, Percent_Identity=28.436018957346, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=654, Percent_Identity=23.5474006116208, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI17136538, Length=595, Percent_Identity=26.218487394958, Blast_Score=126, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 74923; Mature: 74923

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKSLKSNIKKKQADDLFAASETKGRSSARAPGRASGAEADYTAADIEVLEGLEPVRRRP
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHCC
GMYIGGTDEKALHHLFAEVIDNAMDEALAGHATFIVVELAADGFLTVTDNGRGIPVDPHP
CEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEECCCCCCCCCCCCC
KFPKKSALEVIMCTLHSGGKFDSKVYETSGGLHGVGVSVVNALSSHLEVEVARSQNLYRM
CCCCHHHHHHHHEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHEEEECCCCCEEEE
TFERGHPKGKLEDLGRIANRRGTTIRFKPDTEIFGAKAAFKPQRLFKMTRSKAYLFGGVE
HHHCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHCCCCCCCHHHHHHHHHCCEEEEECEE
IRWRCAPELLKGLEDLPPEATFHFPGGLKDYLAAAIHKDTLVHPDIFSGRSGRNGAHGAC
EEEEECHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCE
EWAVAWTADADGFLSSYCNTVPTPDGGTHESGLRSALLRGLKDHAERVGQGKRAGSITSE
EEEEEEECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
DVMVGAAVMLSVFVREPEFQGQTKDRLATAEASRIVEQAIKDPFDHWLSGNPAQANRLLD
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHH
FVIERADERLRRRAEKETSRKSAVKKLRLPGKLADCTNTATEGSELFIVEGDSAGGSAKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCEEEEEECCCCCCCHHH
ARDRKTQAVLPLRGKILNVVSATRDKVTANAQLADLIQALGCGTGTNYREEDLRYSRVII
HHHHHHHEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHCCCEEEEEE
MTDADVDGAHIASLLITFFYRQMPRLIDEGHLYLAVPPLYRLTHGSKTVYARDEAHKDAL
EECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCEEEECCHHHHHHH
LKSEFNANAKVDVGRFKGLGEMMPAQLKETTMDPAKRTMLRVMLLAEDREDTADSVERLM
HHHHCCCCCEEEHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
GTKAEARFAFISDKAVFASEDMLDV
CCCCCCEEEEEECCCEECCCCCCCC
>Mature Secondary Structure
MVKSLKSNIKKKQADDLFAASETKGRSSARAPGRASGAEADYTAADIEVLEGLEPVRRRP
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHCC
GMYIGGTDEKALHHLFAEVIDNAMDEALAGHATFIVVELAADGFLTVTDNGRGIPVDPHP
CEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEECCCCCCCCCCCCC
KFPKKSALEVIMCTLHSGGKFDSKVYETSGGLHGVGVSVVNALSSHLEVEVARSQNLYRM
CCCCHHHHHHHHEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHEEEECCCCCEEEE
TFERGHPKGKLEDLGRIANRRGTTIRFKPDTEIFGAKAAFKPQRLFKMTRSKAYLFGGVE
HHHCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHCCCCCCCHHHHHHHHHCCEEEEECEE
IRWRCAPELLKGLEDLPPEATFHFPGGLKDYLAAAIHKDTLVHPDIFSGRSGRNGAHGAC
EEEEECHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCE
EWAVAWTADADGFLSSYCNTVPTPDGGTHESGLRSALLRGLKDHAERVGQGKRAGSITSE
EEEEEEECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
DVMVGAAVMLSVFVREPEFQGQTKDRLATAEASRIVEQAIKDPFDHWLSGNPAQANRLLD
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHH
FVIERADERLRRRAEKETSRKSAVKKLRLPGKLADCTNTATEGSELFIVEGDSAGGSAKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCEEEEEECCCCCCCHHH
ARDRKTQAVLPLRGKILNVVSATRDKVTANAQLADLIQALGCGTGTNYREEDLRYSRVII
HHHHHHHEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHCCCEEEEEE
MTDADVDGAHIASLLITFFYRQMPRLIDEGHLYLAVPPLYRLTHGSKTVYARDEAHKDAL
EECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCEEEECCHHHHHHH
LKSEFNANAKVDVGRFKGLGEMMPAQLKETTMDPAKRTMLRVMLLAEDREDTADSVERLM
HHHHCCCCCEEEHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
GTKAEARFAFISDKAVFASEDMLDV
CCCCCCEEEEEECCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797224 [H]