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The map label for this gene is ntrX [H]

Identifier: 75675639

GI number: 75675639

Start: 1577353

End: 1578723

Strand: Direct

Name: ntrX [H]

Synonym: Nwi_1447

Alternate gene names: 75675639

Gene position: 1577353-1578723 (Clockwise)

Preceding gene: 75675638

Following gene: 75675640

Centisome position: 46.36

GC content: 61.78

Gene sequence:

>1371_bases
ATGGCACATGACATTCTGATTGTTGACGACGAGGCGGACATTCGCGACCTGGTTGCGGGCATCCTGGAAGATGAGGGGTT
CACGACCCGAACCGCGCGCAACAGCGATGAGGCGCTGGCGGAGGTTTCAAGCCGCCGTCCGCACATGGTGTTTCTCGACA
TCTGGCTGCAAGGCAGCAAGCTCGATGGGCTCCAGCTGCTGGAAATGATCAAGCAGGACAGCCCCGATATGCCGGTCGTG
ATGATCTCCGGTCATGGCAACATCGAAACGGCGGTCGCGGCCATCAAACGCGGCGCCTATGACTTCATCGAAAAGCCGTT
CAAGGCCGATCGGCTGATTCTGGTGGCGACGCGTGCGCTCGAAACGTCGCGCCTGAAGCGCGAGGTCCGTGAACTGAAGC
AGCTCGCGCCGGCGGCAAGCTCGCTGGTTGGGCGTTCGCCATGCATGAACCAGCTTCGGCAAACCATCGAGCGCGCGGCG
AAAGCCAACAGCCGCATCATGATCGTCGGCCCGTCCGGGTCCGGCAAGGAACTGGCGGCGCGAACGCTTCATAATCAGTC
GAGCCGTGCCGAGGGGCCGTTTGTTGTCATCAACGCCGCTGCGATCACGCCGGAGCGAATGGAGTCCGAACTGTTCGGCG
TGGAGCAGGTCGACGGTCAGCAGCCTCGCAAGGCCGGTGCGCTGGAAGAAGCGCACGGCGGGACGCTGTTCATCGACGAG
ATCGGCGATATGCCGCGCGAGACGCAGAACAAGATCATGCGGGTCCTGGTCGATCAGACCTTTCAACGCTCAGGCGGCGC
AACCAAGATCAAGGTGGATGTGCGCATCATTTCGTCGACAGCGCGCAATCTGGAGGAGGAAATTGCAGCGGGCAGGTTCC
GTGAAGATCTCTATCACCGGCTGTCGGTGGTGCCGATCCGCGTTCCGCCATTGTCCGAACGCCGCGAGGATATTCCCGAA
CTGATCGAATTTTTCATGGACCATATCTCCGCCACGAGCGGTTTGCCCAAGCGCCAGATCGGCGATGACGCGATGGCGGT
GCTGCAATCGCATGTCTGGCCGGGCAACGTCCGGCAACTCCGAAACAATGTCGAGCGCGTAATGATCCTGGCAGGCGGAG
GCCCGGAGGTCATCATCACCGCGGATATGTTGCCGCAGGATGTCGGCTCCATGGTCCCGGCGATGCCGTCGGGCGTCAAT
GGCGAGCACATCATGGGCTTGCCGCTTCGCGAGGCCCGCGAGGTTTTCGAGCGCGACTATCTGATCGCTCAGATCAGCCG
CTTCTCCAACAACATCTCCCGGACCGCGGAGTTCGTCGGCATGGAGCGCTCGGCGCTGCATCGCAAACTCAAGGCGCTCG
GCGTCAACTAG

Upstream 100 bases:

>100_bases
GAACCATTGGGGATGATGGAGCGACTAACGGGGATACCCTGACCGGAAAAGAAACAAAGACTGAGGTTGCGACCGGCGGC
TGACGAGGCGGATCCAATTC

Downstream 100 bases:

>100_bases
ATTGTTGCTCTGACGCATGGTCATCCCGCGAAATCGTATTCATCTCGATCGAGAACAGGTAACGCTGAGGTAAACCTGCT
GAATGTCGCGCATCGCTTAT

Product: nitrogen assimilation regulatory protein ntrX

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MAHDILIVDDEADIRDLVAGILEDEGFTTRTARNSDEALAEVSSRRPHMVFLDIWLQGSKLDGLQLLEMIKQDSPDMPVV
MISGHGNIETAVAAIKRGAYDFIEKPFKADRLILVATRALETSRLKREVRELKQLAPAASSLVGRSPCMNQLRQTIERAA
KANSRIMIVGPSGSGKELAARTLHNQSSRAEGPFVVINAAAITPERMESELFGVEQVDGQQPRKAGALEEAHGGTLFIDE
IGDMPRETQNKIMRVLVDQTFQRSGGATKIKVDVRIISSTARNLEEEIAAGRFREDLYHRLSVVPIRVPPLSERREDIPE
LIEFFMDHISATSGLPKRQIGDDAMAVLQSHVWPGNVRQLRNNVERVMILAGGGPEVIITADMLPQDVGSMVPAMPSGVN
GEHIMGLPLREAREVFERDYLIAQISRFSNNISRTAEFVGMERSALHRKLKALGVN

Sequences:

>Translated_456_residues
MAHDILIVDDEADIRDLVAGILEDEGFTTRTARNSDEALAEVSSRRPHMVFLDIWLQGSKLDGLQLLEMIKQDSPDMPVV
MISGHGNIETAVAAIKRGAYDFIEKPFKADRLILVATRALETSRLKREVRELKQLAPAASSLVGRSPCMNQLRQTIERAA
KANSRIMIVGPSGSGKELAARTLHNQSSRAEGPFVVINAAAITPERMESELFGVEQVDGQQPRKAGALEEAHGGTLFIDE
IGDMPRETQNKIMRVLVDQTFQRSGGATKIKVDVRIISSTARNLEEEIAAGRFREDLYHRLSVVPIRVPPLSERREDIPE
LIEFFMDHISATSGLPKRQIGDDAMAVLQSHVWPGNVRQLRNNVERVMILAGGGPEVIITADMLPQDVGSMVPAMPSGVN
GEHIMGLPLREAREVFERDYLIAQISRFSNNISRTAEFVGMERSALHRKLKALGVN
>Mature_455_residues
AHDILIVDDEADIRDLVAGILEDEGFTTRTARNSDEALAEVSSRRPHMVFLDIWLQGSKLDGLQLLEMIKQDSPDMPVVM
ISGHGNIETAVAAIKRGAYDFIEKPFKADRLILVATRALETSRLKREVRELKQLAPAASSLVGRSPCMNQLRQTIERAAK
ANSRIMIVGPSGSGKELAARTLHNQSSRAEGPFVVINAAAITPERMESELFGVEQVDGQQPRKAGALEEAHGGTLFIDEI
GDMPRETQNKIMRVLVDQTFQRSGGATKIKVDVRIISSTARNLEEEIAAGRFREDLYHRLSVVPIRVPPLSERREDIPEL
IEFFMDHISATSGLPKRQIGDDAMAVLQSHVWPGNVRQLRNNVERVMILAGGGPEVIITADMLPQDVGSMVPAMPSGVNG
EHIMGLPLREAREVFERDYLIAQISRFSNNISRTAEFVGMERSALHRKLKALGVN

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=473, Percent_Identity=36.5750528541226, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1788550, Length=463, Percent_Identity=35.85313174946, Blast_Score=288, Evalue=6e-79,
Organism=Escherichia coli, GI1790437, Length=454, Percent_Identity=35.9030837004405, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI1788905, Length=456, Percent_Identity=35.0877192982456, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI87082152, Length=329, Percent_Identity=36.7781155015198, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1789233, Length=322, Percent_Identity=37.5776397515528, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1786524, Length=328, Percent_Identity=35.9756097560976, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1787583, Length=339, Percent_Identity=34.5132743362832, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1789087, Length=323, Percent_Identity=35.9133126934984, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI87082117, Length=235, Percent_Identity=41.2765957446808, Blast_Score=182, Evalue=6e-47,
Organism=Escherichia coli, GI87081872, Length=331, Percent_Identity=32.02416918429, Blast_Score=158, Evalue=9e-40,
Organism=Escherichia coli, GI1789828, Length=263, Percent_Identity=30.4182509505703, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI87081858, Length=305, Percent_Identity=28.1967213114754, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=37.3913043478261, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789809, Length=139, Percent_Identity=30.9352517985612, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50413; Mature: 50281

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHDILIVDDEADIRDLVAGILEDEGFTTRTARNSDEALAEVSSRRPHMVFLDIWLQGSK
CCCCEEEECCCCCHHHHHHHHHCCCCCEECCCCCCHHHHHHHHCCCCCEEEEEEEEECCC
LDGLQLLEMIKQDSPDMPVVMISGHGNIETAVAAIKRGAYDFIEKPFKADRLILVATRAL
CCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHCCCCCCCEEEEEHHHH
ETSRLKREVRELKQLAPAASSLVGRSPCMNQLRQTIERAAKANSRIMIVGPSGSGKELAA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RTLHNQSSRAEGPFVVINAAAITPERMESELFGVEQVDGQQPRKAGALEEAHGGTLFIDE
HHHHCCCCCCCCCEEEEEEEECCHHHHHHHHCCCHHCCCCCCCCCCCCHHCCCCEEEEEC
IGDMPRETQNKIMRVLVDQTFQRSGGATKIKVDVRIISSTARNLEEEIAAGRFREDLYHR
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
LSVVPIRVPPLSERREDIPELIEFFMDHISATSGLPKRQIGDDAMAVLQSHVWPGNVRQL
HEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHCCCCCHHHHH
RNNVERVMILAGGGPEVIITADMLPQDVGSMVPAMPSGVNGEHIMGLPLREAREVFERDY
HCCHHEEEEEECCCCCEEEECCCCHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHH
LIAQISRFSNNISRTAEFVGMERSALHRKLKALGVN
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AHDILIVDDEADIRDLVAGILEDEGFTTRTARNSDEALAEVSSRRPHMVFLDIWLQGSK
CCCEEEECCCCCHHHHHHHHHCCCCCEECCCCCCHHHHHHHHCCCCCEEEEEEEEECCC
LDGLQLLEMIKQDSPDMPVVMISGHGNIETAVAAIKRGAYDFIEKPFKADRLILVATRAL
CCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHCCCCCCCEEEEEHHHH
ETSRLKREVRELKQLAPAASSLVGRSPCMNQLRQTIERAAKANSRIMIVGPSGSGKELAA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RTLHNQSSRAEGPFVVINAAAITPERMESELFGVEQVDGQQPRKAGALEEAHGGTLFIDE
HHHHCCCCCCCCCEEEEEEEECCHHHHHHHHCCCHHCCCCCCCCCCCCHHCCCCEEEEEC
IGDMPRETQNKIMRVLVDQTFQRSGGATKIKVDVRIISSTARNLEEEIAAGRFREDLYHR
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
LSVVPIRVPPLSERREDIPELIEFFMDHISATSGLPKRQIGDDAMAVLQSHVWPGNVRQL
HEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHCCCCCHHHHH
RNNVERVMILAGGGPEVIITADMLPQDVGSMVPAMPSGVNGEHIMGLPLREAREVFERDY
HCCHHEEEEEECCCCCEEEECCCCHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHH
LIAQISRFSNNISRTAEFVGMERSALHRKLKALGVN
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]