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The map label for this gene is pleD [H]

Identifier: 75675624

GI number: 75675624

Start: 1557693

End: 1559066

Strand: Direct

Name: pleD [H]

Synonym: Nwi_1432

Alternate gene names: 75675624

Gene position: 1557693-1559066 (Clockwise)

Preceding gene: 75675623

Following gene: 75675626

Centisome position: 45.79

GC content: 62.95

Gene sequence:

>1374_bases
GTGTCCGCGCGTGTTCTGGTCGTCGACGATGTCCCCGCCAATGTCCGGCTCCTGGAAGCGCGCTTGTCGGCGGAATATTT
CGACGTTTTGACCGCGTCCAATGGCGCGCAGGCATTGGAGACCTGCGCCCGCGCCGAGTGCGACATCATCCTCCTCGACG
TGATGATGCCTGACATGGACGGTTTTGAGGTCTGCCGCCGGCTGAAGGCCAACCCGGCAACCCATTTTATTCCGGTGGTG
ATGATCACCGCGCTGGACAATCCGTCCGACCGTGTGCGCGGCCTTGAGGCCGGCGCCGATGATTTCCTGACCAAGCCGGT
ATCGGATGTCGTGTTGACCGCGCGGGTTCGTTCGTTGACGCGTCTGAAGATGATGACCGACGAACTCCGCCTGCGGGCCA
TGACTTCGCTCGAAATCGGCATGCAGGCGCCGGAGCGCGAGGCGGTCTGCGATAGCGGGAAGGGCGGGCGCATCCTGCTG
GTCGATGACCGTCCCTCGTCGTATGAGCGGCTGGCGTCGATGCTGAGCGCCGAACATGCCGTCGACGTCGAAGCCGACCC
CTCCGAGGCGCTGTTTCGTGCCGCCGAAAGCAATTACGATCTCCTTATCGTCTCGCTCGGGCTGGAAAATTTCGACGGCC
TGCGGCTGTGCAGCCAGGCGCGCTCGCTGGAGCGGACGCGACACGTTCCGATTCTCGCCGTTGCCGACGCCGACGGCAGC
CCGCGATTGCTGCGTGGTCTCGAGATCGGCATCAATGATTATCTGCTGCGGCCGATCGACAGGAACGAACTGCTCGCCCG
CGCGCGCACGCAAATCCGCCGCCGTCGCTATACCGTTCACCTGCGTGACAACGTGGCGAGTTCGATCGAAATGGCCGTCA
CGGACGGACTGACCGGGCTGCACAACCGGCGTTACATGGAAAGCCATCTGACGACGCTGGCCGAGCAGGCCTCGCTGCGC
GGCCAGTCATTGGCGCTCATGATGCTTGATATAGACTACTTCAAGGCGATCAATGACAATTACGGCCATGACGCCGGTGA
CGATGTTCTACGGGAGTTCGCGCTGCGTATCCGCAAAGCTATTCGAGGAATCGATCTGGCCTGCCGTTACGGCGGCGAGG
AGTTCGTGATCGTGATGCCGGAGACCGACCTGCATGTCGCCGGCATGGTGGCCGAGCGCCTGCGCCGCGCGATTGCTGGC
GAACCGTTCTCCATCCACAAGGGGACGAAGCTGATCAAGGTGACCATCTCGATCGGATTGTCGACCCTGGAGCGGAAGGG
GGAGCCTATCTCTGACGTCGTGAAGCGCGCCGATGTCGCGCTTTACCGCGCCAAGCATGACGGCCGAAACCGCGTCGTGG
CGGAGGCGGCTTGA

Upstream 100 bases:

>100_bases
CGCATCCGCGAGGGCGGCTGCGAGGCTTACCTGTCGAAGCCCATTTCGGTCGGCAAGTTCATCGAGACCGTTCGCCGGTT
CGTGGGTTGAGGAGATAAGA

Downstream 100 bases:

>100_bases
TCTCCGCCGCCTGACTCTCGCCGAGGAATGAGAGCCTTCTCGCGTCTGATGGAATCAGAAGCGAAGCCCCATGATCTGGT
GTGTTTTTCTCACGCGAACC

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSARVLVVDDVPANVRLLEARLSAEYFDVLTASNGAQALETCARAECDIILLDVMMPDMDGFEVCRRLKANPATHFIPVV
MITALDNPSDRVRGLEAGADDFLTKPVSDVVLTARVRSLTRLKMMTDELRLRAMTSLEIGMQAPEREAVCDSGKGGRILL
VDDRPSSYERLASMLSAEHAVDVEADPSEALFRAAESNYDLLIVSLGLENFDGLRLCSQARSLERTRHVPILAVADADGS
PRLLRGLEIGINDYLLRPIDRNELLARARTQIRRRRYTVHLRDNVASSIEMAVTDGLTGLHNRRYMESHLTTLAEQASLR
GQSLALMMLDIDYFKAINDNYGHDAGDDVLREFALRIRKAIRGIDLACRYGGEEFVIVMPETDLHVAGMVAERLRRAIAG
EPFSIHKGTKLIKVTISIGLSTLERKGEPISDVVKRADVALYRAKHDGRNRVVAEAA

Sequences:

>Translated_457_residues
MSARVLVVDDVPANVRLLEARLSAEYFDVLTASNGAQALETCARAECDIILLDVMMPDMDGFEVCRRLKANPATHFIPVV
MITALDNPSDRVRGLEAGADDFLTKPVSDVVLTARVRSLTRLKMMTDELRLRAMTSLEIGMQAPEREAVCDSGKGGRILL
VDDRPSSYERLASMLSAEHAVDVEADPSEALFRAAESNYDLLIVSLGLENFDGLRLCSQARSLERTRHVPILAVADADGS
PRLLRGLEIGINDYLLRPIDRNELLARARTQIRRRRYTVHLRDNVASSIEMAVTDGLTGLHNRRYMESHLTTLAEQASLR
GQSLALMMLDIDYFKAINDNYGHDAGDDVLREFALRIRKAIRGIDLACRYGGEEFVIVMPETDLHVAGMVAERLRRAIAG
EPFSIHKGTKLIKVTISIGLSTLERKGEPISDVVKRADVALYRAKHDGRNRVVAEAA
>Mature_456_residues
SARVLVVDDVPANVRLLEARLSAEYFDVLTASNGAQALETCARAECDIILLDVMMPDMDGFEVCRRLKANPATHFIPVVM
ITALDNPSDRVRGLEAGADDFLTKPVSDVVLTARVRSLTRLKMMTDELRLRAMTSLEIGMQAPEREAVCDSGKGGRILLV
DDRPSSYERLASMLSAEHAVDVEADPSEALFRAAESNYDLLIVSLGLENFDGLRLCSQARSLERTRHVPILAVADADGSP
RLLRGLEIGINDYLLRPIDRNELLARARTQIRRRRYTVHLRDNVASSIEMAVTDGLTGLHNRRYMESHLTTLAEQASLRG
QSLALMMLDIDYFKAINDNYGHDAGDDVLREFALRIRKAIRGIDLACRYGGEEFVIVMPETDLHVAGMVAERLRRAIAGE
PFSIHKGTKLIKVTISIGLSTLERKGEPISDVVKRADVALYRAKHDGRNRVVAEAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=161, Percent_Identity=43.4782608695652, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=38.8888888888889, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082007, Length=172, Percent_Identity=38.3720930232558, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=33.1360946745562, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1787262, Length=183, Percent_Identity=35.5191256830601, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=35.8490566037736, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788381, Length=161, Percent_Identity=35.4037267080745, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=35.8490566037736, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=37.2881355932203, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788394, Length=180, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=37.2881355932203, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=38.8429752066116, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87081977, Length=188, Percent_Identity=30.3191489361702, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788085, Length=168, Percent_Identity=33.9285714285714, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787541, Length=165, Percent_Identity=30.9090909090909, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=39.8305084745763, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI2367329, Length=125, Percent_Identity=40, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=34.7457627118644, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788956, Length=181, Percent_Identity=33.7016574585635, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081974, Length=159, Percent_Identity=27.6729559748428, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI145693157, Length=108, Percent_Identity=39.8148148148148, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789149, Length=109, Percent_Identity=34.8623853211009, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790863, Length=118, Percent_Identity=29.6610169491525, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786911, Length=113, Percent_Identity=37.1681415929204, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788713, Length=126, Percent_Identity=32.5396825396825, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1790437, Length=104, Percent_Identity=39.4230769230769, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787229, Length=119, Percent_Identity=31.0924369747899, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI48994928, Length=116, Percent_Identity=37.0689655172414, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788550, Length=127, Percent_Identity=30.7086614173228, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1789402, Length=118, Percent_Identity=33.0508474576271, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI145693140, Length=111, Percent_Identity=35.1351351351351, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790552, Length=93, Percent_Identity=37.6344086021505, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50646; Mature: 50515

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARVLVVDDVPANVRLLEARLSAEYFDVLTASNGAQALETCARAECDIILLDVMMPDMD
CCCEEEEEECCCCCCEEHHHHHHHHHHHHEECCCCHHHHHHHHCCCCCEEEEEECCCCCC
GFEVCRRLKANPATHFIPVVMITALDNPSDRVRGLEAGADDFLTKPVSDVVLTARVRSLT
HHHHHHHHCCCCCHHEEEEEEEEECCCCHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHH
RLKMMTDELRLRAMTSLEIGMQAPEREAVCDSGKGGRILLVDDRPSSYERLASMLSAEHA
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHC
VDVEADPSEALFRAAESNYDLLIVSLGLENFDGLRLCSQARSLERTRHVPILAVADADGS
CCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCC
PRLLRGLEIGINDYLLRPIDRNELLARARTQIRRRRYTVHLRDNVASSIEMAVTDGLTGL
CHHHHHEEECCCHHEECCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHCCCCHH
HNRRYMESHLTTLAEQASLRGQSLALMMLDIDYFKAINDNYGHDAGDDVLREFALRIRKA
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
IRGIDLACRYGGEEFVIVMPETDLHVAGMVAERLRRAIAGEPFSIHKGTKLIKVTISIGL
HCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEEECCH
STLERKGEPISDVVKRADVALYRAKHDGRNRVVAEAA
HHHHHCCCCHHHHHHHHHHHHEEECCCCCCCEEEECC
>Mature Secondary Structure 
SARVLVVDDVPANVRLLEARLSAEYFDVLTASNGAQALETCARAECDIILLDVMMPDMD
CCEEEEEECCCCCCEEHHHHHHHHHHHHEECCCCHHHHHHHHCCCCCEEEEEECCCCCC
GFEVCRRLKANPATHFIPVVMITALDNPSDRVRGLEAGADDFLTKPVSDVVLTARVRSLT
HHHHHHHHCCCCCHHEEEEEEEEECCCCHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHH
RLKMMTDELRLRAMTSLEIGMQAPEREAVCDSGKGGRILLVDDRPSSYERLASMLSAEHA
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHC
VDVEADPSEALFRAAESNYDLLIVSLGLENFDGLRLCSQARSLERTRHVPILAVADADGS
CCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCC
PRLLRGLEIGINDYLLRPIDRNELLARARTQIRRRRYTVHLRDNVASSIEMAVTDGLTGL
CHHHHHEEECCCHHEECCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHCCCCHH
HNRRYMESHLTTLAEQASLRGQSLALMMLDIDYFKAINDNYGHDAGDDVLREFALRIRKA
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
IRGIDLACRYGGEEFVIVMPETDLHVAGMVAERLRRAIAGEPFSIHKGTKLIKVTISIGL
HCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEEECCH
STLERKGEPISDVVKRADVALYRAKHDGRNRVVAEAA
HHHHHCCCCHHHHHHHHHHHHEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA