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The map label for this gene is tuf [H]

Identifier: 75675554

GI number: 75675554

Start: 1498402

End: 1499592

Strand: Direct

Name: tuf [H]

Synonym: Nwi_1362

Alternate gene names: 75675554

Gene position: 1498402-1499592 (Clockwise)

Preceding gene: 75675553

Following gene: 75675555

Centisome position: 44.04

GC content: 61.29

Gene sequence:

>1191_bases
ATGGCCAAAGCAAAGTTTGAACGTAACAAGCCGCATTGCAACATTGGAACGATCGGTCACGTCGACCATGGCAAGACCTC
ATTGACGGCGGCTATCACGAAGGTTCTTGCGGAAGCCGGCGGAGCGACGTTCACGGCATACGACCAGATTGACAAGGCTC
CTGAGGAAAAGGCGCGCGGCATCACGATCTCGACGTCGCATGTCGAGTATGAGACGCCGAACCGCCACTACGCGCATGTC
GACTGTCCGGGCCATGCCGACTACGTGAAGAACATGATCACGGGCGCGGCGCAGATGGACGGCGCGATTCTTGTCGTGTC
GGCTGCGGACGGCCCGATGCCTCAGACGCGCGAGCACATTCTTCTGGCGCGCCAGGTTGGCGTTCCGGCGATCGTGGTGT
TTCTGAACAAGTGCGACATGGTCGACGATCCGGAGCTTCTGGAACTGGTCGAGATGGAGGTCCGGGAGCTTTTGTCGAAG
TACGAGTTTCCCGGCGACGACATTCCGATCATCAAGGGCTCGGCGCTGGCGGCGCTTGAGGATTCCGACGCCAAGCTCGG
CAAGGAAGCGGTGCTTGAACTGATGAAGGCGGTTGACGCCTACATTCCGCAGCCGGAGCGGCCGGTTGACCAGCCGTTCC
TGATGCCGGTCGAGGACGTGTTCTCGATTTCCGGTCGCGGTACGGTTGTGACGGGACGAGTTGAGCGCGGTATTGTCAAG
GTCGGCGAGGAAATCGAGATCGTCGGCATCCGCGAGACGCAGAAGACGACGGTGACGGGCGTGGAGATGTTCCGCAAGCT
TCTCGATCAGGGGCAGGCGGGCGACAACATCGGAGCGCTGTTGCGCGGCACCAAGCGCGAGGACGTGGAGCGCGGTCAGG
TTTTGTGCAAGCCGGGCTCCGTGAAGCCGCACACCAAGTTCAAGGCGGAAGCCTACATCCTGACCAAGGAGGAAGGTGGC
CGTCACACGCCGTTTTTCACCAACTATCGGCCGCAGTTCTATTTCCGCACAACGGATGTGACCGGGGTGGTGCATCTTCC
GGCCGGCACCGAGATGGTGATGCCGGGCGACAATGTCGCGATGGAGGTGCATCTGATCGTGCCGATCGCCATGGAGGAGA
AGCTGCGCTTCGCCATCCGCGAGGGCGGTCGTACCGTCGGCGCAGGCGTCGTCGCCAGCATCATCGAGTAA

Upstream 100 bases:

>100_bases
CGATCATTACGCGGAAGCGCCGGCGAATGTGTCGGCGGAAGTCCAGAAAAAGTTTGCCTGATTGTCGCTGGCGACAGTCG
ACGACTGAACGGAGAGTCAA

Downstream 100 bases:

>100_bases
GGACAAGGGAATTGCGAATAGCGAGTGGCGAATAGAACACCTTCTATTTCGCTATTCGCTACTCACTACTCACTATTCGC
TTTTCAAAGAGAGACACGGC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKVLAEAGGATFTAYDQIDKAPEEKARGITISTSHVEYETPNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKCDMVDDPELLELVEMEVRELLSK
YEFPGDDIPIIKGSALAALEDSDAKLGKEAVLELMKAVDAYIPQPERPVDQPFLMPVEDVFSISGRGTVVTGRVERGIVK
VGEEIEIVGIRETQKTTVTGVEMFRKLLDQGQAGDNIGALLRGTKREDVERGQVLCKPGSVKPHTKFKAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGVVHLPAGTEMVMPGDNVAMEVHLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE

Sequences:

>Translated_396_residues
MAKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKVLAEAGGATFTAYDQIDKAPEEKARGITISTSHVEYETPNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKCDMVDDPELLELVEMEVRELLSK
YEFPGDDIPIIKGSALAALEDSDAKLGKEAVLELMKAVDAYIPQPERPVDQPFLMPVEDVFSISGRGTVVTGRVERGIVK
VGEEIEIVGIRETQKTTVTGVEMFRKLLDQGQAGDNIGALLRGTKREDVERGQVLCKPGSVKPHTKFKAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGVVHLPAGTEMVMPGDNVAMEVHLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE
>Mature_395_residues
AKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKVLAEAGGATFTAYDQIDKAPEEKARGITISTSHVEYETPNRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKCDMVDDPELLELVEMEVRELLSKY
EFPGDDIPIIKGSALAALEDSDAKLGKEAVLELMKAVDAYIPQPERPVDQPFLMPVEDVFSISGRGTVVTGRVERGIVKV
GEEIEIVGIRETQKTTVTGVEMFRKLLDQGQAGDNIGALLRGTKREDVERGQVLCKPGSVKPHTKFKAEAYILTKEEGGR
HTPFFTNYRPQFYFRTTDVTGVVHLPAGTEMVMPGDNVAMEVHLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=57.6142131979695, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI223555963, Length=445, Percent_Identity=30.3370786516854, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI5729864, Length=445, Percent_Identity=30.3370786516854, Blast_Score=172, Evalue=7e-43,
Organism=Homo sapiens, GI4503471, Length=442, Percent_Identity=30.0904977375566, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4503475, Length=443, Percent_Identity=29.5711060948081, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI194018522, Length=323, Percent_Identity=29.7213622291022, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI194018520, Length=323, Percent_Identity=29.7213622291022, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI194097354, Length=323, Percent_Identity=29.7213622291022, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI46094014, Length=323, Percent_Identity=29.4117647058824, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI54607086, Length=316, Percent_Identity=26.8987341772152, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI4503507, Length=269, Percent_Identity=28.996282527881, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI157426893, Length=307, Percent_Identity=27.3615635179153, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI53729339, Length=241, Percent_Identity=31.1203319502075, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI53729337, Length=241, Percent_Identity=31.1203319502075, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=77.2151898734177, Blast_Score=611, Evalue=1e-176,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=77.2151898734177, Blast_Score=610, Evalue=1e-176,
Organism=Escherichia coli, GI2367247, Length=379, Percent_Identity=31.1345646437995, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI48994988, Length=241, Percent_Identity=34.0248962655602, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789108, Length=330, Percent_Identity=28.1818181818182, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=53.75, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.1524547803618, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=448, Percent_Identity=30.3571428571429, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=448, Percent_Identity=30.3571428571429, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI32566303, Length=426, Percent_Identity=29.3427230046948, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=441, Percent_Identity=28.1179138321995, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=345, Percent_Identity=27.8260869565217, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=28.2110091743119, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=342, Percent_Identity=28.3625730994152, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=37.4149659863946, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=274, Percent_Identity=27.007299270073, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=234, Percent_Identity=27.3504273504274, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=68.0904522613065, Blast_Score=549, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=30.1587301587302, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=30.1587301587302, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=346, Percent_Identity=26.3005780346821, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=345, Percent_Identity=27.536231884058, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=268, Percent_Identity=27.6119402985075, Blast_Score=73, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=254, Percent_Identity=25.5905511811024, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=181, Percent_Identity=31.4917127071823, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=59.5419847328244, Blast_Score=498, Evalue=1e-141,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=59.5419847328244, Blast_Score=498, Evalue=1e-141,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=49.4791666666667, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI45553807, Length=448, Percent_Identity=28.5714285714286, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI45553816, Length=448, Percent_Identity=28.5714285714286, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24651721, Length=448, Percent_Identity=28.5714285714286, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17864154, Length=448, Percent_Identity=28.5714285714286, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=28.7305122494432, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=28.7305122494432, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI19922690, Length=302, Percent_Identity=32.1192052980132, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI17137380, Length=338, Percent_Identity=29.8816568047337, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI45550900, Length=339, Percent_Identity=28.023598820059, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24647046, Length=280, Percent_Identity=27.1428571428571, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24647048, Length=280, Percent_Identity=27.1428571428571, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28572034, Length=180, Percent_Identity=35, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43180; Mature: 43049

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKVLAEAGGATFTAYDQIDKAPEEKARG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHCC
ITISTSHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEECEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPAIVVFLNKCDMVDDPELLELVEMEVRELLSKYEFPGDDIPIIKGSALAALE
HHHHHCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEC
DSDAKLGKEAVLELMKAVDAYIPQPERPVDQPFLMPVEDVFSISGRGTVVTGRVERGIVK
CCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECHHHHCHHH
VGEEIEIVGIRETQKTTVTGVEMFRKLLDQGQAGDNIGALLRGTKREDVERGQVLCKPGS
CCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHCCCCEEECCCC
VKPHTKFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGVVHLPAGTEMVMPGDNVA
CCCCCCEEEEEEEEEECCCCCCCCEEECCCCEEEEEEECCEEEEECCCCCEEEECCCCEE
MEVHLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE
EEEEEEEEEEHHHHHHHHHHHCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKVLAEAGGATFTAYDQIDKAPEEKARG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHCC
ITISTSHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEECEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPAIVVFLNKCDMVDDPELLELVEMEVRELLSKYEFPGDDIPIIKGSALAALE
HHHHHCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEC
DSDAKLGKEAVLELMKAVDAYIPQPERPVDQPFLMPVEDVFSISGRGTVVTGRVERGIVK
CCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECHHHHCHHH
VGEEIEIVGIRETQKTTVTGVEMFRKLLDQGQAGDNIGALLRGTKREDVERGQVLCKPGS
CCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHCCCCEEECCCC
VKPHTKFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGVVHLPAGTEMVMPGDNVA
CCCCCCEEEEEEEEEECCCCCCCCEEECCCCEEEEEEECCEEEEECCCCCEEEECCCCEE
MEVHLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE
EEEEEEEEEEHHHHHHHHHHHCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA