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The map label for this gene is cysS

Identifier: 75675421

GI number: 75675421

Start: 1351226

End: 1352659

Strand: Direct

Name: cysS

Synonym: Nwi_1228

Alternate gene names: 75675421

Gene position: 1351226-1352659 (Clockwise)

Preceding gene: 75675418

Following gene: 75675422

Centisome position: 39.72

GC content: 59.07

Gene sequence:

>1434_bases
ATGGACCTGCGTCTTTACGACACCCTGACGAGGCGGAAGCGTGCCTTCACGCCGATCGATCCATCGAACGTGCGGATGTA
TGTCTGCGGTCCGACGGTCTATGACTTTGCCCATATCGGCAATGCGCGGCCGGTCATCGTGTTCGACGTGCTGTTCCGGC
TGCTGCGGCATCTCTACGGCGATGATCATGTCACCTACGCCCGCAACATCACCGACGTCGATGATAAGATCAACGACCGC
GCGGCGCGCGATTTTCCTGATCTGCCGCTGAACGAGGCGATCCGCAAGGTCACCGAAAAGACCGCCGCGCAGTTTCAGGC
CGATGTCGCCGCGCTCGGCTGCCTGCCGCCGACGCACCAGCCGCGCGCCACCGAGTTCGTGCTGCCGCGCACCGACGGCA
AGACTGACATGGTGACGTTTATCAAGCAGCTTATCGCGCGCGGCCATGCTTATGAGGCGGGCGGCGAGGTGCTGTTCGAC
GTGCGGTCGATGCCGGACTACGGCGCGCTGTCCGGCCGCAGGCTGGATGAGCAAAAGGCCGGCGCGCGGGTCGCGGTGGA
TGCGCATAAAAGAAATCCGGCTGACTTCGTGCTGTGGAAGCTGTCATCCGAGAACGAACCGGGCTGGGACAGTCCGTGGG
GCAGGGGGCGGCCGGGCTGGCACATCGAATGTTCGGCGATGAGCACGGCCTATCTTGGCGATGTATTCGACATCCACGGC
GGTGGCCTCGACCTGATCTTCCCGCATCATGAGAACGAGATCGCGCAGTCGCGCTGCGCCCACGGCACGCACGCGATGGC
GAATTACTGGATGCACAACGGCTTCCTGCAGGTCGAAGGCGAGAAGATGTCGAAGTCACTCGGCAACTTCGTGACGATCA
ATGAACTGCTGACGACGGAGAAATTTGGCGGGCGGAAGTGGCCGGGTGAGGTGTTGCGGCTGAATATGTTGCGCACCCAT
TATCGCCAACCGATCGATTGGACGATCAAAGCTTTGGAAGAGAGTGAAACTATTCTCCGTCGCGTCTGCGGGCGAATAAA
ACAGTTTGAGTATGCCCGTCGCGGGATGCCGACGAAAGAGCGATCGGTTCAATTGAACCTTCCACCAGAAATATTGGAGG
CGCTCGTTGATGATCTTAACACTCCGCTGATGCTTTCGAGAATTGGAGAGCTGAGCTACGATGACGCCTTCGACCATGCG
CTATTATCTATTGGTATCGACGTGTTGTCATACGCCCGTTGGTTGCGTTCACAAGAACAGTACGCAAGTTCGAATGTCGA
TGAGTTAGTGGTCGCTCGTCTTGAGGCTAGAATGCGAAAAGATTTCAAGGAATCCGACCGCATCCGGGACGAACTCGCCG
CCATGGGCGTTGTGCTGAAGGACGGTAAGGACGCCGACGGCAAGCCGGTGACGACATGGGAGATCGCGCGATGA

Upstream 100 bases:

>100_bases
GGCCGTCTCTATCTCACGATGAGATCACCGGGCGTCCTGCCAGCGAGCGGAACGGATTTGCATCCCGCTGTCCAGCGGCA
GCGACCTCGGAACGGATTCC

Downstream 100 bases:

>100_bases
CAAAGTCAACGATCCCTCACCCGGCGTCTCGCTGGCGCTCGCCGCCACCCTCTCCCGCAAGGGGAGAGGGAAAGAAGGAG
TTCCCGCGATGACGTGGCGC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MDLRLYDTLTRRKRAFTPIDPSNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHLYGDDHVTYARNITDVDDKINDR
AARDFPDLPLNEAIRKVTEKTAAQFQADVAALGCLPPTHQPRATEFVLPRTDGKTDMVTFIKQLIARGHAYEAGGEVLFD
VRSMPDYGALSGRRLDEQKAGARVAVDAHKRNPADFVLWKLSSENEPGWDSPWGRGRPGWHIECSAMSTAYLGDVFDIHG
GGLDLIFPHHENEIAQSRCAHGTHAMANYWMHNGFLQVEGEKMSKSLGNFVTINELLTTEKFGGRKWPGEVLRLNMLRTH
YRQPIDWTIKALEESETILRRVCGRIKQFEYARRGMPTKERSVQLNLPPEILEALVDDLNTPLMLSRIGELSYDDAFDHA
LLSIGIDVLSYARWLRSQEQYASSNVDELVVARLEARMRKDFKESDRIRDELAAMGVVLKDGKDADGKPVTTWEIAR

Sequences:

>Translated_477_residues
MDLRLYDTLTRRKRAFTPIDPSNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHLYGDDHVTYARNITDVDDKINDR
AARDFPDLPLNEAIRKVTEKTAAQFQADVAALGCLPPTHQPRATEFVLPRTDGKTDMVTFIKQLIARGHAYEAGGEVLFD
VRSMPDYGALSGRRLDEQKAGARVAVDAHKRNPADFVLWKLSSENEPGWDSPWGRGRPGWHIECSAMSTAYLGDVFDIHG
GGLDLIFPHHENEIAQSRCAHGTHAMANYWMHNGFLQVEGEKMSKSLGNFVTINELLTTEKFGGRKWPGEVLRLNMLRTH
YRQPIDWTIKALEESETILRRVCGRIKQFEYARRGMPTKERSVQLNLPPEILEALVDDLNTPLMLSRIGELSYDDAFDHA
LLSIGIDVLSYARWLRSQEQYASSNVDELVVARLEARMRKDFKESDRIRDELAAMGVVLKDGKDADGKPVTTWEIAR
>Mature_477_residues
MDLRLYDTLTRRKRAFTPIDPSNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHLYGDDHVTYARNITDVDDKINDR
AARDFPDLPLNEAIRKVTEKTAAQFQADVAALGCLPPTHQPRATEFVLPRTDGKTDMVTFIKQLIARGHAYEAGGEVLFD
VRSMPDYGALSGRRLDEQKAGARVAVDAHKRNPADFVLWKLSSENEPGWDSPWGRGRPGWHIECSAMSTAYLGDVFDIHG
GGLDLIFPHHENEIAQSRCAHGTHAMANYWMHNGFLQVEGEKMSKSLGNFVTINELLTTEKFGGRKWPGEVLRLNMLRTH
YRQPIDWTIKALEESETILRRVCGRIKQFEYARRGMPTKERSVQLNLPPEILEALVDDLNTPLMLSRIGELSYDDAFDHA
LLSIGIDVLSYARWLRSQEQYASSNVDELVVARLEARMRKDFKESDRIRDELAAMGVVLKDGKDADGKPVTTWEIAR

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=411, Percent_Identity=37.2262773722628, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI10835051, Length=262, Percent_Identity=37.0229007633588, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI21269877, Length=262, Percent_Identity=37.0229007633588, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI303304987, Length=262, Percent_Identity=37.0229007633588, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI62240992, Length=262, Percent_Identity=37.0229007633588, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1786737, Length=491, Percent_Identity=40.7331975560082, Blast_Score=331, Evalue=8e-92,
Organism=Caenorhabditis elegans, GI17509797, Length=345, Percent_Identity=34.4927536231884, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17509795, Length=345, Percent_Identity=34.4927536231884, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6324082, Length=325, Percent_Identity=33.2307692307692, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI19922218, Length=344, Percent_Identity=39.2441860465116, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI19922364, Length=243, Percent_Identity=41.9753086419753, Blast_Score=183, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_NITWN (Q3STA1)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_317842.1
- ProteinModelPortal:   Q3STA1
- SMR:   Q3STA1
- STRING:   Q3STA1
- GeneID:   3676109
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_1228
- NMPDR:   fig|323098.3.peg.1990
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   INARAAC
- ProtClustDB:   PRK00260
- BioCyc:   NWIN323098:NWI_1228-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 54151; Mature: 54151

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 285-285

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRLYDTLTRRKRAFTPIDPSNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHLYG
CCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHHC
DDHVTYARNITDVDDKINDRAARDFPDLPLNEAIRKVTEKTAAQFQADVAALGCLPPTHQ
CCCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PRATEFVLPRTDGKTDMVTFIKQLIARGHAYEAGGEVLFDVRSMPDYGALSGRRLDEQKA
CCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHC
GARVAVDAHKRNPADFVLWKLSSENEPGWDSPWGRGRPGWHIECSAMSTAYLGDVFDIHG
CCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCEEECC
GGLDLIFPHHENEIAQSRCAHGTHAMANYWMHNGFLQVEGEKMSKSLGNFVTINELLTTE
CCEEEEECCCCCHHHHHHHCCCHHHHHHHHHHCCEEEECCHHHHHHHCCEEEHHHHHHHH
KFGGRKWPGEVLRLNMLRTHYRQPIDWTIKALEESETILRRVCGRIKQFEYARRGMPTKE
HCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RSVQLNLPPEILEALVDDLNTPLMLSRIGELSYDDAFDHALLSIGIDVLSYARWLRSQEQ
CEEEECCCHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YASSNVDELVVARLEARMRKDFKESDRIRDELAAMGVVLKDGKDADGKPVTTWEIAR
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCEEECCC
>Mature Secondary Structure
MDLRLYDTLTRRKRAFTPIDPSNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHLYG
CCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHHC
DDHVTYARNITDVDDKINDRAARDFPDLPLNEAIRKVTEKTAAQFQADVAALGCLPPTHQ
CCCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PRATEFVLPRTDGKTDMVTFIKQLIARGHAYEAGGEVLFDVRSMPDYGALSGRRLDEQKA
CCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHC
GARVAVDAHKRNPADFVLWKLSSENEPGWDSPWGRGRPGWHIECSAMSTAYLGDVFDIHG
CCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCEEECC
GGLDLIFPHHENEIAQSRCAHGTHAMANYWMHNGFLQVEGEKMSKSLGNFVTINELLTTE
CCEEEEECCCCCHHHHHHHCCCHHHHHHHHHHCCEEEECCHHHHHHHCCEEEHHHHHHHH
KFGGRKWPGEVLRLNMLRTHYRQPIDWTIKALEESETILRRVCGRIKQFEYARRGMPTKE
HCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RSVQLNLPPEILEALVDDLNTPLMLSRIGELSYDDAFDHALLSIGIDVLSYARWLRSQEQ
CEEEECCCHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YASSNVDELVVARLEARMRKDFKESDRIRDELAAMGVVLKDGKDADGKPVTTWEIAR
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA