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The map label for this gene is thiD [H]

Identifier: 75675275

GI number: 75675275

Start: 1186219

End: 1187034

Strand: Direct

Name: thiD [H]

Synonym: Nwi_1082

Alternate gene names: 75675275

Gene position: 1186219-1187034 (Clockwise)

Preceding gene: 75675272

Following gene: 75675276

Centisome position: 34.87

GC content: 64.22

Gene sequence:

>816_bases
ATGATGACAGTCCCTGTTGCCTTGACCATCGCCGGCTCCGATTCCAGCGGCGGCGCGGGAATCCAGGCCGACCTCAAGAC
ATTCGCCGCGCTTGGTGTTTATGGAGCGTCCGTCATTACGGCGCTGACCGCGCAGAACACGCAAGGCGTCGCCGCCATCC
ATCACGTTCCGGCGCATTTCGTAACCGCGCAGATCGACGCGGTCTTCGGCGATCTCGATGTCGGAGCGCTCAAGATCGGG
ATGGTCGCCCAGGTCGCCGTGATCGATGCCATCGCAGCCGGCCTGTCGCGCTGGGCACCCGCTCATGTCGTGCTTGATCC
GGTGATGGTCGCGACCTCGGGAGCGCATCTGCTGGAGTCCGATGCGATCGAGGCATTGCGCAGCATGCTTGTTCCGCGAG
CGCAACTGTTGACGCCAAATCTTCCGGAGGCCGCAGTTCTGCTCGATGAACCCGTTGCCGGGGGAGAGACTGACATCGTG
CGGCAGGGCCAGCGGCTGCTGGAACTCGGTTGTGCCGCGGTCTTGATCAAGGGCGGGCACATGCACGGCCCCGAGAGCAT
CGATTATCTTGTCACCCGCCAGGGAGCGATGACGTTCGCCGCCCCGCGTGCCGTCACCAGAAATACCCACGGCACCGGCT
GTTCGCTGTCGTCAGCCATCGCCGCAGGTCTGGCCAAAGGCGACGGCATGGAAACCGCCGTGCGCGGAGCGAAGGCTTAC
GTCACGGCGGCGATCGCGGCCGGGGACCGGCTGAACATCGGTCGCGGCCACGGACCGATCCATCATTTTCACCAGTTCGA
GCATTTTCGCTTCTGA

Upstream 100 bases:

>100_bases
TTTGACAGGCGGCCGCTCTAGGTCCTTCACCGTTTCCATGAAGCGGTGAAAGACTTTAGGATCATTATGCTGGCGTGATC
CGACGCCGTTACGCGAGACG

Downstream 100 bases:

>100_bases
TCGACCAGGAAGCGGAACTCCCGCGAAGAGGTCACTCATCTCCGGGTTAAGCCCGAGGACGTGCTTCGATCGAAACGCAG
TAGCCGACCGGCGCAGCCAT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MMTVPVALTIAGSDSSGGAGIQADLKTFAALGVYGASVITALTAQNTQGVAAIHHVPAHFVTAQIDAVFGDLDVGALKIG
MVAQVAVIDAIAAGLSRWAPAHVVLDPVMVATSGAHLLESDAIEALRSMLVPRAQLLTPNLPEAAVLLDEPVAGGETDIV
RQGQRLLELGCAAVLIKGGHMHGPESIDYLVTRQGAMTFAAPRAVTRNTHGTGCSLSSAIAAGLAKGDGMETAVRGAKAY
VTAAIAAGDRLNIGRGHGPIHHFHQFEHFRF

Sequences:

>Translated_271_residues
MMTVPVALTIAGSDSSGGAGIQADLKTFAALGVYGASVITALTAQNTQGVAAIHHVPAHFVTAQIDAVFGDLDVGALKIG
MVAQVAVIDAIAAGLSRWAPAHVVLDPVMVATSGAHLLESDAIEALRSMLVPRAQLLTPNLPEAAVLLDEPVAGGETDIV
RQGQRLLELGCAAVLIKGGHMHGPESIDYLVTRQGAMTFAAPRAVTRNTHGTGCSLSSAIAAGLAKGDGMETAVRGAKAY
VTAAIAAGDRLNIGRGHGPIHHFHQFEHFRF
>Mature_271_residues
MMTVPVALTIAGSDSSGGAGIQADLKTFAALGVYGASVITALTAQNTQGVAAIHHVPAHFVTAQIDAVFGDLDVGALKIG
MVAQVAVIDAIAAGLSRWAPAHVVLDPVMVATSGAHLLESDAIEALRSMLVPRAQLLTPNLPEAAVLLDEPVAGGETDIV
RQGQRLLELGCAAVLIKGGHMHGPESIDYLVTRQGAMTFAAPRAVTRNTHGTGCSLSSAIAAGLAKGDGMETAVRGAKAY
VTAAIAAGDRLNIGRGHGPIHHFHQFEHFRF

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=260, Percent_Identity=52.6923076923077, Blast_Score=281, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6325378, Length=282, Percent_Identity=35.1063829787234, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6324517, Length=277, Percent_Identity=35.3790613718412, Blast_Score=135, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6324997, Length=277, Percent_Identity=36.101083032491, Blast_Score=133, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 27823; Mature: 27823

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTVPVALTIAGSDSSGGAGIQADLKTFAALGVYGASVITALTAQNTQGVAAIHHVPAHF
CEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCHHH
VTAQIDAVFGDLDVGALKIGMVAQVAVIDAIAAGLSRWAPAHVVLDPVMVATSGAHLLES
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHEECCCCHHHHH
DAIEALRSMLVPRAQLLTPNLPEAAVLLDEPVAGGETDIVRQGQRLLELGCAAVLIKGGH
HHHHHHHHHHCCHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHEEEEECCC
MHGPESIDYLVTRQGAMTFAAPRAVTRNTHGTGCSLSSAIAAGLAKGDGMETAVRGAKAY
CCCCCCCEEEEECCCCEEEECCCHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
VTAAIAAGDRLNIGRGHGPIHHFHQFEHFRF
EEEEEECCCEECCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MMTVPVALTIAGSDSSGGAGIQADLKTFAALGVYGASVITALTAQNTQGVAAIHHVPAHF
CEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCHHH
VTAQIDAVFGDLDVGALKIGMVAQVAVIDAIAAGLSRWAPAHVVLDPVMVATSGAHLLES
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHEECCCCHHHHH
DAIEALRSMLVPRAQLLTPNLPEAAVLLDEPVAGGETDIVRQGQRLLELGCAAVLIKGGH
HHHHHHHHHHCCHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHEEEEECCC
MHGPESIDYLVTRQGAMTFAAPRAVTRNTHGTGCSLSSAIAAGLAKGDGMETAVRGAKAY
CCCCCCCEEEEECCCCEEEECCCHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
VTAAIAAGDRLNIGRGHGPIHHFHQFEHFRF
EEEEEECCCEECCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]