Click here to switch to the map view.

The map label for this gene is capI [H]

Identifier: 75675267

GI number: 75675267

Start: 1176200

End: 1177219

Strand: Direct

Name: capI [H]

Synonym: Nwi_1074

Alternate gene names: 75675267

Gene position: 1176200-1177219 (Clockwise)

Preceding gene: 75675265

Following gene: 75675268

Centisome position: 34.57

GC content: 59.61

Gene sequence:

>1020_bases
ATGCCGGATCATCCGATATTGGTTACCGGCGCGGCGGGCTTCATCGGGTTTCACGTCGCCGCCCGGCTCCTGAAACAGGG
GCATCGGGTTGTCGGCATTGACAGTCTCAACGATTATTATGATCCCGCGCTCAAGGAGTGCCGGCTTGAGATCCTGCGCG
GGGATTCGCGCTTCCGTTTCGTCAAGTCCGACCTGGCCGATCGCGAGGCGACGGCCGCCTTGTTCGCCGAACATCATCTT
TCGGTCGTGCTCCATCTCGCCGCGCAGGCGGGGGTGCGATATTCGTTGCGGAATCCCCACGCCTACGTCGATTCCAACCT
CACGGCGTTTGCAAACGTTCTCGAAGGCTGTCGGCACGCCTCCTGTCCGCACCTGCTCTTTGCGTCATCGTCCTCCGTCT
ACGGTGCGAACACAAAGCTTCCCTTTTCCGTTCATGACAATGTCGATCACCCGATCAGCCTCTATGCGGCGACGAAGAAG
TCGAACGAATTGATGGCGCACGCCTACAGTCACCTCTACCGCGTTCCGACGACGGGATTGCGTTTTTTCACGGTCTATGG
CCCCTGGTATCGTCCCGACATGGCGCTCTACAAGTTCGCCGATGCGATCGTGGGGGGGCAGCCGATCAAGCTGTTCAACC
ATGGCAACATGCAGCGGGATTTCACGTTCGTCGATGACGTGGTGGAAGCCGTTGTCCGCCTGATCGATCGCGCTCCACAG
CCTCATGCAAGCTGGTCGGGCGATGCCTCGGATGCGGGGACCAGTTCGGCGCCATGGCGAATCTACAATATCGGCAACAA
CAAGCCGGCGGAGTTGATGGGCGTCGTCGCTCTTCTGGAGAAGGCGCTCGGACGATCCGCGCAAAAGGAATTGCTGCCGA
TGCAACCCGGCGACGTACAGGCGACGTTTGCGGACATCGATGACCTCGCCCGTGACGTCGGTTTCCGTCCCTCGACCTCG
CTGGAAGATGGTATCCATCGTTTTGCCGACTGGTATTGTCGGTATCATCGGGTCAGTTGA

Upstream 100 bases:

>100_bases
CGGTACCCACTTCGGTCGAAAACGCTATAACCTCGCGGGCCGCGCCTCACCTTTCCCGAGCGGTCACATCTCTTGAGGAA
CGAAATTCTTGAGGAACGAA

Downstream 100 bases:

>100_bases
TGCGGCTGTCGGGGTTTTCATGATCCGATCGGGTGCTTTCGTCGGAAAGCTTGTGGTGTCCTTTGATCCAGGGTTCGCCT
TTGATCCTGGGTTCGCATGG

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MPDHPILVTGAAGFIGFHVAARLLKQGHRVVGIDSLNDYYDPALKECRLEILRGDSRFRFVKSDLADREATAALFAEHHL
SVVLHLAAQAGVRYSLRNPHAYVDSNLTAFANVLEGCRHASCPHLLFASSSSVYGANTKLPFSVHDNVDHPISLYAATKK
SNELMAHAYSHLYRVPTTGLRFFTVYGPWYRPDMALYKFADAIVGGQPIKLFNHGNMQRDFTFVDDVVEAVVRLIDRAPQ
PHASWSGDASDAGTSSAPWRIYNIGNNKPAELMGVVALLEKALGRSAQKELLPMQPGDVQATFADIDDLARDVGFRPSTS
LEDGIHRFADWYCRYHRVS

Sequences:

>Translated_339_residues
MPDHPILVTGAAGFIGFHVAARLLKQGHRVVGIDSLNDYYDPALKECRLEILRGDSRFRFVKSDLADREATAALFAEHHL
SVVLHLAAQAGVRYSLRNPHAYVDSNLTAFANVLEGCRHASCPHLLFASSSSVYGANTKLPFSVHDNVDHPISLYAATKK
SNELMAHAYSHLYRVPTTGLRFFTVYGPWYRPDMALYKFADAIVGGQPIKLFNHGNMQRDFTFVDDVVEAVVRLIDRAPQ
PHASWSGDASDAGTSSAPWRIYNIGNNKPAELMGVVALLEKALGRSAQKELLPMQPGDVQATFADIDDLARDVGFRPSTS
LEDGIHRFADWYCRYHRVS
>Mature_338_residues
PDHPILVTGAAGFIGFHVAARLLKQGHRVVGIDSLNDYYDPALKECRLEILRGDSRFRFVKSDLADREATAALFAEHHLS
VVLHLAAQAGVRYSLRNPHAYVDSNLTAFANVLEGCRHASCPHLLFASSSSVYGANTKLPFSVHDNVDHPISLYAATKKS
NELMAHAYSHLYRVPTTGLRFFTVYGPWYRPDMALYKFADAIVGGQPIKLFNHGNMQRDFTFVDDVVEAVVRLIDRAPQP
HASWSGDASDAGTSSAPWRIYNIGNNKPAELMGVVALLEKALGRSAQKELLPMQPGDVQATFADIDDLARDVGFRPSTSL
EDGIHRFADWYCRYHRVS

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=347, Percent_Identity=24.207492795389, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI42516563, Length=335, Percent_Identity=24.4776119402985, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI56237023, Length=342, Percent_Identity=25.1461988304094, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI56118217, Length=342, Percent_Identity=25.1461988304094, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI189083684, Length=342, Percent_Identity=25.1461988304094, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI1788353, Length=354, Percent_Identity=25.1412429378531, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI48994969, Length=355, Percent_Identity=25.6338028169014, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786974, Length=353, Percent_Identity=24.0793201133144, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1788589, Length=281, Percent_Identity=27.7580071174377, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71982035, Length=352, Percent_Identity=26.4204545454545, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71982038, Length=354, Percent_Identity=26.5536723163842, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17568069, Length=345, Percent_Identity=22.6086956521739, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=336, Percent_Identity=25.5952380952381, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI115532424, Length=338, Percent_Identity=23.3727810650888, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=343, Percent_Identity=24.4897959183673, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19923002, Length=353, Percent_Identity=24.929178470255, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356223, Length=336, Percent_Identity=26.4880952380952, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37594; Mature: 37463

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDHPILVTGAAGFIGFHVAARLLKQGHRVVGIDSLNDYYDPALKECRLEILRGDSRFRF
CCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEECCCHHHCCHHHHHHHHHHHCCCCHHHH
VKSDLADREATAALFAEHHLSVVLHLAAQAGVRYSLRNPHAYVDSNLTAFANVLEGCRHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEECCCCHHHHHHHHHHHHCC
SCPHLLFASSSSVYGANTKLPFSVHDNVDHPISLYAATKKSNELMAHAYSHLYRVPTTGL
CCCEEEEECCCCCCCCCCCCCEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCE
RFFTVYGPWYRPDMALYKFADAIVGGQPIKLFNHGNMQRDFTFVDDVVEAVVRLIDRAPQ
EEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
PHASWSGDASDAGTSSAPWRIYNIGNNKPAELMGVVALLEKALGRSAQKELLPMQPGDVQ
CCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCE
ATFADIDDLARDVGFRPSTSLEDGIHRFADWYCRYHRVS
EHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDHPILVTGAAGFIGFHVAARLLKQGHRVVGIDSLNDYYDPALKECRLEILRGDSRFRF
CCCCEEEECCHHHHHHHHHHHHHHCCCEEEEECCCHHHCCHHHHHHHHHHHCCCCHHHH
VKSDLADREATAALFAEHHLSVVLHLAAQAGVRYSLRNPHAYVDSNLTAFANVLEGCRHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEECCCCHHHHHHHHHHHHCC
SCPHLLFASSSSVYGANTKLPFSVHDNVDHPISLYAATKKSNELMAHAYSHLYRVPTTGL
CCCEEEEECCCCCCCCCCCCCEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCE
RFFTVYGPWYRPDMALYKFADAIVGGQPIKLFNHGNMQRDFTFVDDVVEAVVRLIDRAPQ
EEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
PHASWSGDASDAGTSSAPWRIYNIGNNKPAELMGVVALLEKALGRSAQKELLPMQPGDVQ
CCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCE
ATFADIDDLARDVGFRPSTSLEDGIHRFADWYCRYHRVS
EHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]